BSP siRNA 的构建及其对成骨样细胞株MC3T3-E1相关基因表达的影响

目的 构建BSP干扰表达载体,有效沉默小鼠成骨样细胞MC3T3-E1 Subclone 14细胞的BSP基因表达,研究其对MC3T3-E1 Subclone 14增殖分化过程中OCN等成骨相关基因表达的影响.方法 ①利用互联网资源针对BSP基因mRNA序列设计四条可能的小干扰RNA(siRNA),将这四条小干扰RNA插...     

Conjugation of bromosulphthalein (BSP) with reduced glutathione in the gastrointestinal tract in vertebrate species

Present study has revealed the existence of bromosulphthalein-glutathione conjugating enzyme activity in the gastrointestinal tract of all the vertebrate species tested. The enzyme activity demonstrated in the intestine is comparable to that in kidneys.It is inferred that like the liver and kidney, the gastrointestinal tract may have detoxicating/conjugating function.     

ECT联合NSE、NTx、BSP检测对肺癌骨转移的诊断价值

目的探讨放射性核素骨显像(ECT)联合神经元特异性烯醇化酶(NSE)、Ι型胶原交联氨基末端肽(NTx)、骨涎蛋白(BSP)诊断肺癌骨转移的价值。方法随机选取行ECT的肺癌患者82例为实验组,根据是否发生骨转移分为骨转移组(≤2个骨转移灶,>2个骨转移灶)和无骨转移组;另选30例健康体检者为对照组,应用罗氏Cobas601全自动化学发光仪、ELISA法分别检测外周血清中NSE、BSP、NTx的含量。结果实验组血清中NSE、BSP、NTx含量明显高于对照组(t=6.47、4.92、6.88;P<0.01)。实验组中ECT显示发生骨转移45例(骨转移组),显像正常无骨转移37例(无骨转移组),骨转移组与无骨转移组的基本情况比较差异无统计学意义(P>0.05)。无骨转移组NSE、NTx、BSP的含量均高于对照组,差异无统计学意义(t=0.8、1.28、1.34,P=0.43、0.20、0.18);骨转移组血清相关指标含量明显高于对照组(t=9.13、9.47、9.99,P<0.01)。小于或等于2个骨转移灶患者的NSE、BSP、NTx水平明显低于大于2个骨转移灶患者的血清相关指标水平(t=2.28、2.61、2.65,P=0.028、0.012、0.014)。ECT联合外周血清相关指标含量检测的敏感性和特异性可以达到98.3%和95.5%。结论 ECT联合血清中NSE、BSP、NTx水平检测可以提高肺癌骨转移的检出率。     

The effect of sodium taurocholate on the hepatic metabolism of sulfobromophthalein sodium (BSP). The role of bile flow

The influence of bile salts on the hepatic metabolism of sulfobromophthalein sodium (BSP) was studied in the perfused rat liver. During sodium taurocholate infusions, hepatic uptake of BSP from plasma was increased and appeared to be related to an enhanced transit of BSP from liver into bile. BSP-glutathione conjugation was not affected by the bile salt infusions, although bile salts inhibited the enzyme system in vitro.The major effect of bile salts was to increase the BSP transport maximum (Tm). When sodium taurocholate was infused in saline at a rate of 30 mumoles/hr, both bile flow and the BSP Tm increased, and remained at peak levels of 1.5 +/-0.12 mul/min per g liver and 21 +/-3.0 mug/min per g liver, respectively. In contrast, during saline infusion alone both levels were significantly lower (1.06 +/-0.17 mul/min per g liver and 15.8 +/-4.16 mug/min per g liver, respectively), and both fell progressively after the 2nd hr of perfusion. This decline in bile flow and BSP Tm was associated with a decrease in biliary bile salt excretion and was reversed by adding bile salts to the perfusate. Since the biliary concentration of BSP remained within a narrow range in all experiments, the BSP Tm was primarily determined by the rate of bile flow.Dependence of BSP Tm on the rate of bile production was further confirmed by changing the temperature of the perfusate during a constant infusion of taurocholate. BSP Tm paralleled temperature-induced changes in bile flow irrespective of changes in the level of bile salt excretion.Since the biliary concentration of BSP remained within a narrow range in all experiments, the concentrating capacity for BSP in bile may be the major limiting factor in BSP transport. Thus two independent factors appear to determine the BSP Tm: the bile BSP concentration, and the rate of bile production.Because taurocholate enhanced BSP transport only when it increased bile production, its effect on the BSP Tm appears to be attributable to its choleretic properties.     

Solution properties of sulfobromophthalein sodium (BSP) compounds alone and in association with sodium taurocholate (TC).

A series of in vitro studies have been performed utilizing the techniques of ultracentrifugation, freezing point depression, vapor pressure osmometry, and spectrophotometry, to study the colloid-chemical characteristics of various sulfobromophthalein sodium (BSP) compounds in aqueous solution and to evaluate the possibility of a direct physicochemical interaction between BSP and taurocholate (TC). The results of these studies indicate that: (1) BSP compounds self-associate in aqueous solution to form polymolecular aggregates. These aggregates are larger with conjugated BSP, where the aggregation number appears to increase with the concentration of BSP, compared with the more polar glutathione conjugate of BSP; (2) There is a marked physicochemical interaction between unconjugated BSP and TC and a much smaller effect between the bile salt and conjugated BSP. This interaction was minor between BSP and glycodeoxycholate or taurodehydrocholate but was reproduced fully by glycocholate. Such an interaction between BSP and TC may have physiologic importance and may help to explain the previously noted facilitated excretion of BSP observed after infusion of TC in experimental animals.     

Comparison of sulfobromophthalein (BSP) and sulfobromophthalein glutathione (BSP-GSH) disposition under conditions of altered liver function and in the isolated perfused rat liver.

Disposition of sulfobromophthalein (BSP) and sulfobromophthalein glutathione (BSP-GSH) was compared in control, phenobarbital, or alpha-naphthylisothiocyanate-(ANIT)-treated rats, and in the isolated perfused rat liver preparation. After dye administration, BSP-GSH was found to have a more rapid early plasma disappearance rate, a more rapid appearance in the liver, and a greater rate of biliary excretion both in vivo and in the isolated perfused liver, than that of BSP. In considering these observations, it is concluded that hepatic uptake as well as biliary excretion of BSP-GSH is faster than that of BSP. Comparing BSP and phenobarbital, augmentation or reduction in plasma dye concentration, mean plasma half-life (2 to 30 minutes), hepatic dye content, and bile dye concentration, were of the same order of magnitude for both dyes. However, with dye infusion of 3.6 mumoles per kilogram per minute, phenobarbital significantly enhanced the rate of biliary excretion of BSP but not BSP-GSH and ANIT treatment had a greater inhibitory effect on biliary excretion of BSP-GSH than BSP.