蕈样肉芽肿AgNORs分析

探讨ＡｇＮＯＲｓ方法在茜肉芽肿研究中的意义。方法：应用银染一步法对皮肤２０例ＭＦ及１０例偏平苔藓的ＡｇＮＯＲｓ进行定量分析。结果：ＭＦＡｇＮＯＲｓ的平均值为４．０１±０．９０,ＬＰ为１．８２±０．３０,两者之间差别非常显著。而在ＡｇＮＯＲｓ形态上,ＭＦ为卫星型,ＬＰ为核仁型,结论：Ａｇ－ＮＯＲｓ形态上,ＭＦ为卫星型,ＬＰ核为仁型,结论：ＡｇＮＯＲｓ计数及形态学观察可以做为ＭＦ诊断的一个有效指标用于     

宫颈良恶性变AgNORs变化的研究

本文应用ＡｇＮＯＲｓ染色技术对３５例宫颈良恶性病变中的细胞核内ＡｇＮＯＲｓ的数量、形态、大小及分布进行光镜观察与对比分析。结果表明：炎性增生组细胞核内Ａｇ－ＮＯＲｓ数量少于不典型增生组，ＡｇＮＯＲｓ颗粒均分布于中位，颗粒呈圆形、卵圆形。在宫颈鳞癌中Ⅱ级、Ⅲ级ＡｇＮＯＲｓ明显多于Ⅰ级，也多于良性病变（Ｐ＜０．０１），并随细胞恶性程度的增高分布于核周的ＡｇＮＯＲｓ增多（Ｐ＜０．０１），并出现不规则形。因此，ＡｇＮＯＲｓ的检测在宫颈良恶性病变及肿瘤分级中有一定的参考价值。     

非霍奇金淋巴瘤细胞增殖活性和组织学恶性程度及预后的关系

１３１例非霍奇金淋巴瘤（ＮＨＬ）按国际工作分类分低、中、高度恶性（ＬＧ、ＭＧ、ＨＧ）３组。对ＮＨＬ和１４例反应性增生淋巴组织（ＲＬＨ）作核分裂计数（ＭＦ），银染核仁组成区（ＡｇＮＯＲｓ）计数，并用ＭＢＣ法检测部分病例的增殖细胞核抗原（ＰＣＮＡ）和Ｋｉ＿６７抗原。ＭＦ、ＡｇＮＯＲｓ、ＰＣＮＡ及Ｋｉ＿６７检测结果有较好相关性（ｒ值０．６１２，Ｐ＜０．０１），均和ＮＨＬ恶性度呈正相关，ＬＧ、ＭＧ、ＨＧ及ＲＬＨ组间有显著性差异（Ｐ＜０．０１，但ＬＧ和ＲＬＨ组Ｐ＞０．０５），表明细胞增殖活性的检测有助于了解ＮＨＬ恶性度，但不能区分ＬＧ和ＲＬＨ。ＭＧ中弥漫小裂细胞型（ＤＳＣ）细胞增殖活性低于同组其它类型（Ｐ＜０．０５），但与ＬＧ无显著性差异（Ｐ＞０．０５），预后相对较好，拟属ＬＧ。ＭＦ、ＡｇＮＯＲｓ和ＰＣＮＡ值越高，生存期越短，表明细胞增殖活性有助于预测ＮＨＬ预后。ＭＦ和ＡｇＮＯＲｓ经济易行；Ｋｉ６７全面反映增殖细胞的数量，仅用于冰冻切片；ＰＣＮＡ可用于石蜡切片，便于回顾性研究。     

核仁组成体区相关蛋白与胃癌淋巴结转移关系的研究

本文用特殊染色方法研究了核仁组成体区相关嗜银蛋白（ＡｇＮｏＲｓ）和间质反应与胃癌淋巴结转移的关系。结果表明，胃癌细胞中的ＡｇＮＯＲｓ数量（２．３５±０．８５）明显多于正常幽门腺细胞（１．２１±０．４２），直径明显小于正常幽门腺细胞（Ｐ＜０．０１）；淋巴结转移组癌细胞ＡｇＮＯＲｓ数量（２．２９±０．８０）明显多于无转移组者（１．７６±０．３３）（Ｐ＜０．０１）；淋巴结转移组癌间质淋巴细胞浸润程度和胶原纤维反应程度明显低于无转移组（Ｐ＜０．０１）。提示ＡｇＮＯＲｓ数目与癌细胞增殖活性及转移能力有关。     

核仁形成区蛋白变化与白血病疗效及预后判断的临床观察

对７８例急性白血病和慢性粒细胞白血病患者于化疗前后取其骨髓细胞，应用Ｈｏｗｅｌ一步法［１］嗜银染技术观察其核仁形成区蛋白颗粒（ＡｇＮＯＲｓ）数目及形态变化，并与２２例非血液肿瘤疾病进行对照．结果：急性白血病组及慢性粒细胞白血病组在未缓解期其ＡｇＮＯＲｓ数目明显高于缓解期组（Ｐ＜００１），且颗粒粗大，呈弥散型及巨块型；而已缓解期患者ＡｇＮＯＲｓ数较对照组仍明显增多（Ｐ＜００５）．提示：ＡｇＮＯＲｓ可作为白血病疗效判断、估计预后的一项指标     

红景天对大鼠脑缺血再灌注时自由基损伤保护作用的实验研究

目的：探讨红景天对脑缺血再灌注时自由基损伤的保护作用,为脑缺血再灌注损伤的防治提供新的药物。方法：用４ＶＯ法复制大鼠实验模型,分为假手术组（ＳＡＭ）、单纯缺血组（ＩＳ）、缺血再灌注组（ＩＲ）、药物预防后缺血再灌注组（Ｒ＋ＩＲ）、缺血再灌注后药物治疗组（ＩＲ＋Ｒ）,分别观察了大鼠脑组织中ＬＰＯ、ＳＯＤ、Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ的变化。结果：①ＩＳ组及ＩＲ组大鼠脑组织中ＬＰＯ含量均显著高于ＳＡＭ组（Ｐ＜０．０５,Ｐ＜０．０１）。ＳＯＤ含量均低于ＳＡＭ组（Ｐ＜０．０５,Ｐ＜０．０１）,Ｒ＋ＩＲ组及ＩＲ＋Ｒ组大鼠脑组织中ＬＰＯ含量显著降低（Ｐ＜０．０５）,而ＳＯＤ含量却显著升高（Ｐ＜０．０１）。②ＩＲ组大鼠脑组织中Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ活性明显低于ＳＡＭ组（Ｐ＜０．０１）,Ｒ＋ＩＲ组及ＩＲ＋Ｒ组较ＩＲ组升高（Ｐ＜０．０５,Ｐ＜０．０１）。结论：红景天对大鼠脑缺血再灌注时自由基损伤具有一定的保护作用。     

乳腺癌雌激素受体表达与增殖细胞核抗原表达和AgNOR计数关系的研究

目的研究乳腺癌雌激素受体（ＥＲ）表达与增殖细胞核抗原（ＰＣＮＡ）表达和ＡｇＮＯＲ计数关系。方法采用ＡｇＮＯＲ染色技术和免疫组化Ｓ－Ｐ法，用抗ＥＲ单克隆抗体及抗ＰＣＮＡ单克隆抗体（ＰＣ１０），研究５０例乳腺癌ＥＲ与ＰＣＮＡ表达及ＡｇＮＯＲ计数。结果３３例乳腺癌ＥＲ阳性者与１７例ＥＲ阴性者相比，ＰＣＮＡ表达低（Ｐ＜０．０５），ＡｇＮＯＲ平均颗粒少（分别为４．９１±１．６９，６．５２±２．１３，Ｐ＜０．０１）。结论乳腺癌ＥＲ表达与ＰＣＮＡ表达及ＡｇＮＯＲ计数有相关关系，ＰＣＮＡ表达率及ＡｇＮＯＲ计数对评估乳腺癌预后有一定意义     

睑板腺癌和霰粒肿核仁组成区相关嗜银蛋白的检测

采用银染技术分别对２０例睑板腺癌和霰粒肿病人进行核仁组成区相关嗜银蛋白（ＡｇＮＯＲｓ）定量检测。结果表明，睑板腺癌的ＡｇＮＯＲｓ计数明显高于霰粒肿组，差异有极显著性（Ｐ＜０．０１）；睑板腺癌组的ＡｇＮＯＲｓ总面积明显高于霰粒肿组，差异有极显著性（Ｐ＜０．０１）。提示ＡｇＮＯＲｓ技术对睑板腺癌的诊断和鉴别诊断有参考价值。     

细胞增殖指标对判断脑膜瘤生物学行为的意义

运用ＤＮＡ图像分析（ＩＡＴ），核仁组成区嗜银染色技术（ＡｇＮＯＲ），增殖细胞核抗原（ＰＣＮＡ）及Ｋｉ－Ｓ单克隆抗体免疫组化染色对９３例脑膜瘤外科切除标本进行观察。结果显示恶性腋膜瘤（ＭＭ）、有复发的良性脑膜瘤（ＲＢＭ）、有局部浸润的良性脑膜瘤（ＩＢＭ）增殖能力高于无复发、无浸润的单纯良性脑膜瘤（ＳＢＭ）（Ｐ＜０．０１），良性脑膜瘤的复发或浸润行为与细胞增殖潜能有关。上述四种方法中Ｋｉ－Ｓ是一种敏感性较高的简便方法：ＡｇＮＯＲ不仅敏感性高，且重复性和稳定性好。结果进一步表明，当ＡｇＮＯＲ颗粒数大于或等于２．８时，良性脑膜瘤复发的可能性较大（Ｐ＜０．０５）。     

皮肤恶性黑色素瘤AgNOR的图象分析

目的：建立ＡｇＮＯＲ方法对皮肤良性痣细胞痣与恶性黑色素瘤进行鉴别。方法：用胶银染色一步法,对皮肤１６例恶性黑色素瘤及４５例良性痣细胞痣的ＡｇＮＯＲ进行定量观察。结果：恶性黑色素瘤每个细胞核的ＡｇＮＯＲ数值为９１６±２８２,痣细胞痣每个细胞核的ＡｇＮＯＲ数值为３５０±０７７,两者之间差别有非常显著性意义（（Ｐ＜００１）。结论：ＡｇＮＯＲ计数可作为鉴别诊断皮肤恶性黑色素瘤与良性痣细胞痣的一个有效的指标。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.