构树叶的化学成分

为研究构树叶(Broussonetia papyrifera)的化学成分，用Diaion HP-20，Toyopearl HW-40C，Sephadex LH-20，silica gel等柱色谱方法进行分离，根据其理化性质和波谱数据鉴定化合物结构。分离得到了19个化合物，分别鉴定为芹菜素(1)，芹菜素-7-O-β-D-吡喃葡糖苷(2)，柯伊利素-7-O-β-D-吡喃葡糖苷(3)，芹菜素-7-O-β-D-吡喃葡糖醛酸苷(4)，牡荆素-7-O-β-D-吡喃葡糖苷(5)，木犀草素(6)，5,7,4′-三羟基-6-C-［a-L-鼠李糖(1→2)］-β-D-葡糖黄酮碳苷(7)，5,7,4′-三羟基-8-C-［α-L-鼠李糖(1→2)］-β-D-葡糖黄酮碳苷(8)，异牡荆素(9)，牡荆素(10)，苯甲酸苯甲酯-2,6-二-O-β-D-吡喃葡糖苷(11)，(2R,3R,5R,6S,9R)-3-羟基-5,6-环氧-β-紫罗兰醇-2-O-β-D-葡糖苷(12)，(2R,3R,5R,6S,9R)-3-羟基-5,6-环氧-乙酰-β-紫罗兰醇-2-O-β-D-葡糖苷(13)，ficustriol (14)，(6S,9S)-玫瑰花苷(15)，3β-羟基-5α,6α-环氧-β-紫罗兰酮-2α-O-β-D-葡糖苷(16)，icariside B₁ (17)，sammangaoside A (18)，3-羟基-5α,6α-环氧-β-紫罗兰酮(19)。化合物11、12、13为新化合物，其余化合物为首次从该属植物中分离得到。     

杜仲叶中倍半萜及降碳倍半萜的分离与鉴定

目的 研究植物杜仲(Eucommia ulmoides Oliver)叶中的化学成分.方法 综合运用硅胶柱色谱、反相硅胶柱色谱和Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱等方法进行系统分离,根据化合物的理化性质及其波谱数据确定化合物的结构.结果 从杜仲叶80％(体积分数)乙醇提取物中分离得到了12个倍...     

Sesquiterpenes from the aerial parts of Artemisia vestita Wall.

A chemical investigation of the aerial parts of Artemisia vestita Wall. led to the isolation of 12 known sesquiterpenes, including 2 furan-containing sesquiterpenoids and 10 eudesmane sesquiterpene lactones. Their structures were identified as negunfurol (1), schensianol A (2), artemine (3), erivanin (4), 1,5-diepi-artemin (5), acetylartemin (6), naphtho[1,2-b]furan-2(3H)-one, 6-(acetyloxy)decahydro-9a-hydroxy-3,5a-dimethyl-9-methylene-(3S,3aS,5aS,6S,9aS,9bS) (7), naphtho[1,2-b]furan-2(3H)-one, 6-(acetyloxy)-3a,4,5,5a,6,7,8,9b-octahydro-8-hydroxy-3,5a,9-trimethyl- (3S,3aS,5aR,6S,8S,9bS) (8), isoerivanin (9), barrelierin (10), (11S)-1-oxoeudesm-4(14)-eno-13,6α-lactone (11), 1-epi-dehydroisoeranin (12), respectively. All of these compounds were isolated from Artemisia vestita for the first time, and compounds 1 and 2 were isolated from the genus Artemisia for the first time.     

Sesquiterpene lactones from Inula hookeri

Four new sesquiterpene lactones, (1 S,5 R,6 S,7 S,8 R,9 R,10 S,11 S)-6-acetoxy-9-hydroxy-4-oxo-pseudoguai-2(3)-en-12,8-olide, (1 S,2 R,5 R,6 S,7 R,8 S,10 R)-6-acetoxy-2-ethoxy-4-oxo-pseudoguai-11(13)-en-12,8-olide, (1 S,2 R,5 R,6 S,7 R,8 S,10 R)-6-acetoxy-2-hydroxy-4-oxo-pseudoguai-11(13)-en-12,8-olide, and 14-acetoxy-1 β,5 α,7 αH-4 β-hydroxy-guai-9(10),11(13)-dien-12,8 α-olide, along with 26 known sesquiterpene lactones, were isolated from the whole plants of Inula hookeri C. B. Clarke. Their structures were established based on spectroscopic methods including HRESIMS, 1D and 2D NMR, and CD techniques. All compounds were evaluated for their cytotoxic activities against HepG2, HeLa, PC-3, and MGC-803 cell lines by CCK-8 assay. Some of the isolates, especiallly pseudoguaianolides and guaianolides, exhibited significant cytotoxicities against these four examined cell lines.     

穿心莲根的化学成分研究

为研究常用中药爵床科植物穿心莲(Andrographis paniculata)根的化学成分,运用各种色谱方法从安徽临泉产穿心莲根的80%乙醇提取物中分离并鉴定了28个化合物,其中20个黄酮:5,5'-二羟基-7,8,2'-三甲氧基黄酮(1)、5-羟基-7,8,2',6'-四甲氧基黄酮(2)、5,3'-dihydroxy-7,8,4',-trimethoxyflavone(3)、2'-羟基-5,7,8-三甲氧基黄酮(4)、5-羟基-7,8,2',3',4'-五甲氧基黄酮(6)、wightin(7)、5,2',6'-trihydroxy-7-methoxyflavone 2'-O-β-D-glucopyranoside(8)、5,7,8,2'-四甲氧基黄酮(10)、5-羟基-7,8-二甲氧基二氢黄酮(11)、5-羟基-7,8-二甲氧基黄酮(12)、5,2'-二羟基-7,8-二甲氧基黄酮(13)、5-羟基-7,8,2',5'-四甲氧基黄酮(14)、5-羟基-7,8,2',3'-四甲氧基黄酮(15)、5-羟基-7,8,2'-三甲氧基黄酮(16)、5,4'-二羟基-7,8,2',3'-四甲氧基黄酮(17)、二氢黄芩新...     

蒙古黄芪化学成分研究

目的研究蒙古黄芪的化学成分。方法采用硅胶柱色谱、凝胶柱色谱方法进行分离纯化,利用波谱分析法结合理化性质确定化合物结构。结果从蒙古黄芪的乙酸乙酯和正丁醇萃取部分中共分离得到14个化合物,分别鉴定为(6aR,11R)-9,10-二甲氧基紫檀烷-3-O-β-D-葡萄糖苷(1),(3R)-2′-羟基-3′,4′-二甲氧基异黄烷-7-O-β-D-葡萄糖苷(2),5′-羟基-3′-甲氧基异黄酮-7-O-β-D-葡萄糖苷(3),芒柄花苷(4),(6aR,11R)-3-羟基-9,10-二甲氧基紫檀烷(5),(3R)-7,2′-二羟基-3′,4′-二甲氧基异黄烷(6),5′,7-二羟基-3′-甲氧基异黄酮(7),芒柄花素(8),黄芪甲苷(9),黄芪皂苷II(10),蔗糖(11),腺嘌呤核苷(12),十六烷酸单甘油酯(13),十六烷酸(14)。结论化合物5,7,11～14为首次从黄芪属植物中分离得到,化合物6为首次从蒙古黄芪中分离得到。     

Norsesquiterpenoid glucosides and a rhamnoside of pyrrolizidine alkaloid from Tephroseris kirilowii

Four new glycosylated compounds have been isolated from the whole plant of Tephroseris kirilowii, including (-)-(1R,5R,6S,7R,8S)-8-O-beta-D-glucopyranosyloxy-7-hydroxy-6-(2-hydroxypropan-2-yl)-9-methylenebicyclo[4.3.0]non-3-one (tephroside A, 1), (-)-(1R,5R,6R,8R)-6-(2-O-beta-D-glucopyranosyloxypropan-2-yl)-8-hydroxy-9-methylenebicyclo[4.3.0]non-3-one (tephroside B, 2), thesinine-4'-O-alpha-L-rhamnoside (3), and p-coumaric acid 4-O-alpha-L-rhamnoside (4), together with the known roseoside. The structures of the new compounds were established by means of spectroscopic analysis.     

当归化学成分的分离与鉴定

目的研究当归Angelica sinensis(Oliv.)Diels的化学成分,为其药理作用的研究奠定物质基础。方法采用硅胶柱色谱及制备薄层色谱等方法进行分离纯化,根据理化性质和波谱数据(1H-NMR、13C-NMR等)鉴定化合物的结构。结果分离得到13个化合物,分别鉴定为:正丁基苯酞(3-butylphthalide,1)、4羟基3丁基苯酞(4-hydroxy-3-butylphthalide,2)、2-methoxy-2-(4'-hydroxyphenyl)ethanol(3)、反式阿魏酸(ferulic acid,4)、厚朴酚(magnolol,5)、黄芩苷(baicalin,6)、2″O(2甲基丁酰基)异当药黄素[2″O(2-methylbutyryl)-isoswertisin,7]、harman(8)、1-ace-tyl-β-carboline(9)、酒渣碱(flazine,10)、菠菜甾醇(α-spinasterol,11)、异欧前胡素(isoimperatorin,12)、发卡二醇[3(R),8(S)-falcarindiol,13]。结论化合物3、7～9、11为首次从该属植物中分离得到,化合物5为首次从该植物中分离得到。     

Chemical constituents of the aerial parts of Glycyrrhiza uralensis and their inhibitory activities against PTP1B and α-glucosidase

In the present study, a total of 11 compounds were isolated from the aerial parts of Glycyrrhiza uralensis, including two new compounds, glycyuralin Q (1) and glycyuralin R (2), and nine known compounds, including licoriphenone (3), orobol (4), trifoliol (5), 7,2′,4′-trihydroxy-5-methoxy-3-arylcoumarin (6), 11-hydroxy-9(Z),12(Z)-octadecadienoic acid (7), 11-hydroxy-9(E),12(E)-octadecadienoic acid (8), licoricone (9), glycyrin (10), and 2′-hydroxyformononetin (11). Structures of the new compounds were identified by 1D, 2D NMR and HR-MS data analyses. Compounds 1, 2 and 10 showed potent inhibitory activities against PTP1B, with IC₅₀ values of 1.43, 4.71 and 3.79 μM, respectively. Compounds 2, 4 and 10 inhibited α-glucosidase with IC₅₀ values of 13.61, 11.13 and 17.48 μM, respectively.     

Antioxidant constituents and a new triterpenoid glycoside from<Emphasis Type="Italic">Flos Lonicerae</Emphasis>

As a component of our continuing investigations into herb-derived antioxidant agents, we have evaluated the antioxidant effects of Flos Lonicerae (Lonicera japonica flowers), via 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, total reactive oxygen species (ROS), hydroxyl radical (*OH), and peroxynitrite (ONOO-) assays. Among the methanolic extract and the dichloromethane, ethyl acetate, n-butanol, and water fractions, the EtOAc fraction of Flos Lonicerae exhibited marked scavenging/inhibitory activities, as follows: IC50 values of 4.37, 27.58 +/- 0.71, 0.47 +/- 0.05, and 12.13 +/- 0.79 microg/mL in the DPPH, total ROS, ONOO-, and *OH assays, respectively. Via a bioactivity-guided fractionation approach, a new triterpenoid glycoside, oleanolic acid 28-O-alpha-L-rhamnopyranosyl-(1-->2)-[beta-D-xylopyranosyl(1-->6)]-beta-D-glucopyranosyl ester (12), along with eleven known compounds, including chrysoeriol (1), luteolin (2), 5-hydroxymethyl-2-furfural (3), caffeic acid (4), protocatechuic acid (5), chrysoeriol 7-O-beta-D-glucopyranoside (6), isorhamnetin 3-O-beta-D-glucopyranoside (7), kaempferol 3-O-beta-D-glucopyranoside (8), quercetin 3-O-beta-D-glucopyranoside (9), hederagenin 3-O-alpha-L-arabinopyranoside (10), and luteolin 7-O-beta-D-glucopyranoside (11), were isolated from the EtOAc fraction. The structures of isolated compounds 1-12 were elucidated via spectroscopic analyses. Compound 12 was isolated from a natural source for the first time. Compounds 2, 4, 5, 7, 9, and 11 evidenced marked scavenging activities, with IC50 values of 2.08-11.76 microM for DPPH radicals, and 1.47-6.98 microM for ONOO-.     

Flavonoid constituents of Melicope triphylla MERR.II

From the leaves of Melicope triphylla MERR., three new flavonoids (1-3) were isolated, together with nine known flavonoids, 4',5-dihydroxy-3,3',7- trimethoxyflavone (4), 5-hydroxy-3,3',4',7-tetramethoxyflavone (5), 3,3',4',5,7-pentamethoxyflavone (6), 7-hydroxy-3,3',4',5,8-pentamethoxyflavone (7), 3,3',4',5,7,8-hexamethoxyflavone (8), 5-hydroxy-3,7,8-trimethoxy-3',4'-methylenedioxyflavone (9), 7-hydroxy-3,5,8- trimethoxy-3',4'-methylenedioxyflavone (10), 3,5,7,8-tetramethoxy-3',4'-methylenedioxyflavone (11), and 5-hydroxy- 3,6,7,8-tetramethoxy-3',4'-methylenedioxyflavone (12). The structures of 1, 2, and 3 were established as 5-hydroxy-3,7-dimethoxy-3',4'-methylenedioxyflavone, 5-hydroxy-7-isopentenyloxy-3,8-dimethoxy-3',4'-methylenedioxyfl avone, and 4'-hydroxy-7-isopentenyloxy-3,3',5,8-tetramethoxyflavone by their respective chemical and spectral data. The 20 flavonoids isolated from this plant were examined for the piscicidal activities.     

刺山柑果实的化学成分研究

目的研究刺山柑果实的化学成分。方法通过硅胶、sephadex LH-20及反相硅胶(Rp-18)柱层析等色谱方法进行分离,根据理化性质和光谱数据进行结构鉴定。结果从刺山柑果实85%的乙醇提取物中分离鉴定了13个化合物,分别为:二氢-4羟基-5-羟甲基-2(3H)呋喃酮(1)、7-hydroxy-2-oxoindolin-3-ylaceticacid(2)、吲哚-3-甲醛(3)、尿嘧啶(4)、山柰酚芸香苷(5)、芦丁(6)、(6S)-hydroxy-3-oxo-a-ionol(7)、spionosideB(8)、β-谷甾醇(9)、β-胡萝卜苷(10)、β-胡萝卜苷-6′-硬脂酸酯(11)、肌醇(12)和尿苷(13)。结论化合物1～3,12和13为首次从该属植物中分离得到。     

Flavonoids from<Emphasis Type="Italic">Iris spuria</Emphasis> (Zeal) cultivated in Egypt

A new 12a-dehydrorotenoid 1, 11-dihydroxy-9, 10-methylenedioxy-12a-dehydrorotenoid (1), together with a new isoflavonoid glycoside tectorigenin-7-O-beta-glucosyl-4'-O-beta-glucoside (3), were isolated and identified from the rhizomes of I. spuria (Zeal). In addition, 4 known compounds, tectorigenin (2) tectorigenin-7-O-beta-glucosyl (1 --> 6) glucoside (4), tectoridin (a tectorigenin-7-O-beta-glucoside) (5) and tectorigenin-4'-O-beta-glucoside (6) were isolated and identified for the first time from this plant. The structures of the isolated compounds were determined by spectroscopic methods (UV, IR, 1H, 13C-NMR, DEPT, HMQC, NOESY, and HMBC experiments and MS spectrometry) and by comparison with literature data of known compounds. Compounds 2, 4, 5, and 6 are reported for the first time from this plant through the present study.     

Phytochemical constituens of<Emphasis Type="Italic">Cirsium setidens</Emphasis> Nakai and their cytotoxicity against human cancer cell lines

Five terpenes (1-5), three fatty acids (6-8), two sterols (9 and 11), and a monogalactosyldiacyl glycerol (10) were isolated from the methylene chloride extract of the aerial part of Cirsium setidens. Their chemical structures were determined to be alpha-tocopherol (1), 25-hydroperoxycycloart-23-en-3beta-ol (2), 24-hydroperoxycycloart-25-en-3beta-ol (3), mokko lactone (4), transphytol (5), 9,12,15-octadecatrienoic acid (6), 9,12-octadecadienoic acid (7), hexadecanoic acid (8), acylglycosyl beta-sitosterol (9), (2R)-1,2-O-(9z,12z,15z-dioctadecatrienoyl)-3-O-beta-D-galactopyranosyl glycerol (10) and beta-sitosterol glucoside (11) by spectral evidences. Compound 3 exhibited significant cytotoxic activity against five human cancer cell lines with its ED50 values ranging from 2.66 to 11.25 microM.     

巴戟天根皮中的醌类成分的分离与鉴定

目的研究巴戟天(Morinda officinalisHow.)化学成分。方法运用多种色谱学方法对巴戟天根皮体积分数70%乙醇提取物的化学成分进行分离,并根据光谱数据鉴定化合物的结构。结果从该植物中分离得到17个化合物,分别鉴定为rubiasin A(1)、rubiasin B(2)、2-羟基-1-甲氧基蒽醌(2-hydroxy-1-methoxy-anthraquinone,3)、3-羟基-1-甲氧基-2-甲基蒽醌(3-hydroxy-1-methoxy-2-methyl-anthraquinone,4)、1,3-二羟基-2-甲氧基蒽醌(1,3-dihydroxy-2-methoxy-anthraquinone,5)、2-甲基蒽醌(2-methyl-anthraquinone,6)、1,3-二羟基-2-甲基蒽醌(1,3-dihydroxy-2-methyl-anthraqui-none,7)、2-羟甲基蒽醌(2-hydroxymethyl-anthraquinone,8)、3-羟基-1,2-二甲氧基蒽醌(3-hydroxy-1,2-dimethoxy-anthraquinone,9)、1,8-二羟基-3-甲氧基-6-甲基蒽醌(1,8-dihydroxy-3-methoxy-6-methyl-anthraquinone,10)、苯乙醇-O-β-D-吡喃葡萄糖苷(2-phenylethyl-O-β-D-glucopyranoside,11)、2-丁醇-O-β-D-吡喃葡萄糖苷(sec-butyl-O-β-D-glucopyranoside,12)、3,4-二羟基苯乙醇(3,4-di-hydroxyphenylethanol,13)、3-(4-羟基-苯基)-1,2-丙二醇(3-(4-hydroxyphenyl)-1,2-propandiol,14)、阿魏酸(ferulic acid,15)、熊果酸(ursolic acid,16)、β-谷甾醇(β-sitosterol,17)。结论化合物1,2,11~14为首次从巴戟天属植物中分离得到。     

金疮小草化学成分的分离与鉴定

目的对金疮小草的化学成分进行研究。方法采用硅胶吸附柱色谱、ODS柱色谱、制备薄层色谱和半制备型高效液相色谱分离纯化,根据1H-NMR、13C-NMR等谱学数据进行结构鉴定。结果从金疮小草干燥全草的甲醇提取物的乙酸乙酯萃取物中分离得到15个单体化合物,分别鉴定为ajugacumbin A(1)、ajugacumbin B(2)、ajuganipponin B(3)、ajugamarin F4(4)、ajugarin I(5)、ajugamarin A1(6)、ajugamarin A1 chlorhydrin(7)、芹菜素(apigenin,8)、木犀草素(luteolin,9)、金合欢素(acacetin,10)、咖啡酸甲酯(methyl caffeate,11)、4-hydroxy-4-(3-oxo-1-butenyl)-3,5,5-trimethylcyclohex-2-en-1-one(12)、香草酸(vanillic acid,13)、黑麦草内酯(loliolide,14)和(10E,15Z)-9,12,13-trihydroxyoctadeca-10,15-dienoic acid(15)。结论其中化合物11和12为首次从筋骨草属中分离得到,化合物7和15为首次从金疮小草中分离得到。     

Synthesis of DL-hydroxybenzenamides as anticonvulsants

The anticonvulsant activity of some DL-hydroxybenzenamides is described. The following compounds from this series were prepared and tested: DL-2-hydroxy-2-(3'-bromophenyl)butyramide (4, CAS 620950-12-1), DL-2-hydroxy-2-(4'-bromophenyl)butyramide (5, CAS 620950-13-2), DL-2-hydroxy-2-(3'-nitrophenyl)butyramide (6, CAS 620950-14-3), DL-2-hydroxy-2-phenyl hexanamide (7, CAS 63002-05-1), DL-2-hydroxy-2-(3',4'-dichlorophenyl)hexanamide (8, CAS 863976-06-1), DL-3-hydroxy-3-(4'-bromophenyl)pentanamide (9, CAS 620950-16-5), DL-3-hydroxy-3-phenyl-heptanamide (10, CAS 863976-08-3) and DL-4-hydroxy-4-(4'-bromophenyl)hexanamide (11,CAS 620950-18-7). Compounds 4, 5, 9 and 11 exhibited significant activity in seizures induced by pentylenetetrazol. Incorporation of bromine in the phenyl ring increased their potency. Compounds 4, 5, 9 and 11 exhibited a similar anticonvulsant activity as the reference drug phenobarbital (CAS 50-06-6).     

Lignan and terpene constituents from the aerial parts of saussurea pulchella

Chromatographic separation of the MeOH extract from the aerial parts of Saussurea pulchella led to the isolation of seven terpenes (1-4, 11-13), and eight phenolics (5-10, 14-15). Their structures were determined by spectroscopic means to be (3S)-3-O-(3',4'-diangeloyl-beta-D-glucopyranosyloxy)-3,7-trimethylocta-1,6-diene (1), 7delta-methoxy- 4(14)- oppositen-1beta-ol (2) 4(15)- eudesmene-1beta, 6alpha-diol (3), 3alpha-hydroxy-5, 6-epoxy-7-megastigmen-9-one (4), (+)-syringaresinol (5), (7S, 8R, 8'R)-5,5'-dimethoxylariciresinol (6), 8alpha-hydroxypinoresinol (7), (7'R, 8'R)-2,2'- dimethoxy-4- (3-hydroxyl-propenyl)-4'-(1,2,3-trihydroxypropyl)-biphenyl ether (8), 4-allyl-2,6- dimethoxyphenyl glucoside (9), 2-methoxy-4-(2-propenyl)phenyl beta-D-glucoside (10), (-)-oplopan-4-one- 10alpha-O-beta-D-glucoside (11), linalyl-O-beta-D-glucoside (12), amarantholidoside IV (13), (+)-1-hydroxypinoresinol 1-O-beta-D-glucoside (14), and syringin (15). Compounds 1-3 and 8-13 were first isolated from the genus Saussurea. The isolated compounds were examined for cytotoxic activity against four human cancer cell lines in vitro using the sulforhodamin B bio assay method.     

Chemical constituents of the root ofDystaenia takeshimana and their anti-inflammatory activity

In our ongoing search for bioactive compounds originating from the endemic species in Korea, we found that the hexane and EtOAc fractions of the MeOH extract from the root of Dystaenia takeshimana (Nakai) Kitagawa (Umbelliferae) showed cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) dual inhibitory activity by assessing their effects on the production of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) in mouse bone marrow-derived mast cells. By activity-guided fractionation, five coumarins, viz. psoralen (2), xanthotoxin (3), scopoletin (4), umbelliferone (5), and (+)-marmesin (6), together with beta-sitosterol (1), were isolated from the hexane fraction, and two phenethyl alcohol derivatives, viz. 2-methoxy-2-(4'-hydroxyphenyl)ethanol (7) and 2-hydroxy-2-(4'-hydroxyphenyl)ethanol (8), three flavonoids, viz. apigenin (9), luteolin (10), and cynaroside (11), as well as daucosterol (12) were isolated from the EtOAc fraction using silica gel column chromatography. In addition, D-mannitol (13) was isolated from the BuOH fraction by recrystallization. Two of the coumarins, scopoletin (4) and (+)-marmesin (6), the two phenethyl alcohol derivatives (7, 8) and the three flavonoids (9-11) were isolated for the first time from this plant. Among the compounds isolated from this plant, the five coumarins as well as the three flavonoids showed COX-2/5-LOX dual inhibitory activity. These results suggest that the anti-inflammatory activity of D. takeshimana might in part occur via the inhibition of the generation of eicosanoids.     

Cerebrosides and terpene glycosides from the root of<Emphasis Type="Italic">aster scaber</Emphasis>

Three cerebrosides 2, 3, and 5 and two terpene glycosides 1 and 4 have been isolated from the methanol extract of the root of Aster scaber. Their structures were determined as 3-O-beta-D-glucuronopyranosyl-oleanolic acid methyl ester (1), (2S, 3S, 4R, 2'R, 8Z, 15'Z)-N-2'-hydroxy-15'-tetracosenoyl-1-O-beta-D-glucopyranosyl-4-hydroxy-8-sphingenine (2), (2S, 3S, 4R, 8Z)-N-octadecanoyl-1-O-beta-D-glucopyranosyl-4-hydroxy-8-sphingenine (3), 1alpha-hydroxy-6beta-O-beta-D-glucosyl-eudesm-3-ene (4), and (2S, 3S, 4R, 2'R, 8Z)-N-2'-hydroxy-hexadecanoyl-1-O-beta-D-glucopyranosyl-4-hydroxy-8-sphingenine (5) on the basis of spectroscopic methods.