高血压家族史对人群血浆甘油三酯水平的影响

目的探讨高血压家族史对人群血浆甘油三酯水平的影响。方法在NGT375例、IGT328例、NIDDM315例人群中询问高血压家族史,测定血浆甘油三酯、血糖、胰岛素水平。测量身高、体重,分析阳性高血压家族史与血浆甘油三酯水平的关系。结果阳性高血压家族史组甘油三酯水平显著高于无高血压家族史组（1．85±0．01mmol／L,1．46±0．01mmol／L,P＜0．0001）,但年龄（55．1±0．7岁,473±0．4岁）、体重指数（27．9±0．29,25．4±0．13）、血糖水平（8．39±0．27mmol／L,7．19±0．08mmol／L）前组也显著高于后组（P＜0．001）。Pearson相关分析显示血浆甘油三酯水平与高血压家族史、年龄、性别、BMI、血糖水平、胰岛素敏感性显著相关,多因素逐步回归分析显示调整性别、胰岛素敏感性及糖耐量状态影响后,高血压家族史与血浆甘油三酯水平独立相关（P＜0．05）。结论阳性高血压家族史是高甘油三酯血症发病的危险因素,该人群是筛查高甘油三酯血症的重点。     

The -1535 promoter variant of the visfatin gene is associated with serum triglyceride and HDL-cholesterol levels in Japanese subjects

Visfatin is a novel adipocytokine that is expressed by the visceral fat cells. We investigated the role of genetic variation in the visfatin gene in the pathophysiology of type 2 diabetes and clinical variables in Japanese subjects. The 11 exons, and the promoter region of the visfatin gene were screened for single nucleotide polymorphisms (SNPs) by PCR-direct sequencing. We found SNPs in the promoter region (SNP - 1535T>C), exon 2 (SNP + 131C>G, Thr44Arg), and exon 7 (SNP + 903G>A). The allele and genotype frequencies of these SNPs showed no significant differences between 200-448 diabetic and 200-333 control subjects. However, the -1535T/T genotype was associated with lower serum triglyceride levels (T/T vs. T/C + C/C (p = 0.015) and T/T vs. C/C (p = 0.043)) and higher HDL-cholesterol levels (T/T vs. C/C, p = 0.0496) in the nondiabetic subjects. Reporter gene assay of 3T3-L1 adipocytes revealed that the promoter activity of -1535T and -1535C was similar, suggesting that the observed association may reflect linkage disequilibrium between -1535T>C and causative variations of the visfatin gene.     

Influence of serum homocysteine level on coronary atherosclerosis in Japanese

OBJECTIVES: This study investigated the relationship between serum homocysteine level and coronary artery disease in Japanese. METHODS: Serum homocysteine level was measured in 200 consecutive patients who underwent coronary angiography for the assessment of ischemic heart disease. Patients with acute myocardial infarction were excluded, so 197 patients were included in this study. The patients were classified into four groups based on number of diseased vessels identified by coronary angiography: no significant stenosis group (non-vessel group), one-vessel group, two-vessel group, and three-vessel group. More than 50% stenosis was defined as diseased vessels. RESULTS: Serum homocysteine level in the three-vessel group (13.5 +/- 8.0 microM) was significantly higher than that in the non-vessel group (9.9 +/- 2.7 microM), one-vessel group (9.1 +/- 2.3 microM), and two-vessel group (10.4 +/- 3.3 microM). Patients were classified into quartile groups according to the serum homocysteine level. The number of diseased vessels and frequency of three-vessel disease tended to be higher with increasing serum homocysteine level. There was no significant relationship between serum homocysteine level and coronary risk factors (diabetes mellitus, hyperlipidemia, smoking habit) except hypertension. Multivariate analysis for the predictor of number of diseased vessels showed diabetes mellitus, hypertension, and serum homocysteine level were independent predictors. CONCLUSIONS: Elevation of plasma homocysteine level is related to the severity of coronary artery disease in Japanese.     

Plasma apoprotein changes after selective inhibition of hepatic triglyceride lipase in rat

Selective inhibition of hepatic triglyceride lipase (HTGL) by specific antibodies in rats led to an altered VLDL apoprotein composition. Apoprotein analysis by isoelectric focusing revealed a new protein band in VLDL and an increase in apoprotein E (apo E) content. Apoproteins in LDL and HDL remained unchanged. Electronmicroscopy showed a significant increase in particle size of VLDL from 452 to 497 A with no significant changes in LDL and HDL diameters.     

Effects of febuxostat on serum urate level in Japanese hyperuricemia patients

Abstract

Objective. We assessed the efficacy and adverse effects of febuxostat in male hyperuricemia patients. Subjects and methods. This was a 12-week, multicenter, open-label, uncontrolled study. The enrolled subjects were 89 hyperuricemic male patients (12 overexcretors, 56 normal excretors, and 21 underexcretors). The endpoint was percent change in serum urate level. Results. The concentration of urate in serum before and 12 weeks after beginning administration of febuxostat in the overexcretors was 9.34 ± 1.48 and 5.59 ± 1.17 mg/dl, respectively, while those were 8.59 ± 1.24 and 5.41 ± 1.35 mg/dl, respectively, in the normal excretors, and 8.29 ± 1.01and 5.11 ± 1.71 mg/dl, respectively, in the underexcretors. After 12 weeks, the rate of change in serum urate after beginning administration of febuxostat was ? 0.384 ± 0.186 in the overexcretors, ? 0.368 ± 0.128 in the normal excretors, and ? 0.365 ± 0.217 in the underexcretors, with no significant differences among them. A common adverse event related to febuxostat was gout flare. Conclusion. Febuxostat effectively reduced the concentration of urate in serum in hyperuricemic patients regardless of the level of uric acid excreted in urine without severe adverse effects.     

缬沙坦对高血压病患者血浆胰岛素水平的影响

目的 观察缬沙坦(商品名代文,瑞士诺华制药有限公司提供)对高血压病(EH)患者胰岛素敏感性的影响。方法 38例高血压病患者根据血压高低分别给予40～80mg,每天一次口服。治疗前及治疗8周后,分别测空腹血糖(FBS)及空腹血浆胰岛素(FSI),计算胰岛素敏感指数(ISI),并与35例性别、年龄、体重指数相同的健康者比较。结果 两组FPG均正常,无显著性差异,EH组治疗前(FSI)显著升高(P<0.01),ISI显著下降(P<0.01)。缬沙坦治疗8周后,FSI显著下降(P<0.05),ISI显著增高(P<0.05)。结论 高血压病患者存在高胰岛素血症,缬沙坦在稳定降压的同时不同程度的改善高胰岛素血症。     

Effects of serum triglyceride level within 48 hours after hospitalization on the complications of acute pancreatitis

<正>Objective To explore the effects of serum triglyceride( STAG) level within 48 hours after hospitalization on the complications of acute pancreatitis( AP).Methods From January 2012 to June 2016,1 006 hospitalized patients diagnosed with AP at the Third People’ s Hospital of Chengdu were collected.According to the STAG level within 48 hours after hospitalization,     

遗传与环境因素对人体血浆纤维蛋白原水平的影响及临床意义

为探讨遗传与环境因素对血浆纤维蛋白原 ( Fg)的影响及临床意义 ,选择 10 5例血浆 Fg升高者 (高 Fg组 )按 1∶ 1配比设置对照 ,随访比较高 Fg(高 Fg组 )和低 Fg(低 Fg组 )患者及其一级亲属冠心病 ( CHD)发生情况。结果体重指数、高脂肪饮食、吸烟、体力活动少、高血压、甘油三酯、高密度脂蛋白、低密度脂蛋白及一级亲属血浆 Fg水平等因素进入回归方程 ;高 Fg组一级亲属 Fg水平 [( 6 .1± 0 .8g/ L) ]高于低 Fb组一级亲属 [( 4 .4±0 .5 g/ L) ];血浆 Fg浓度高者 CHD发病率 ( 2 4 % )明显高于低 Fg者 ( 10 % )。提示体重指数、高脂肪饮食、吸烟、体力活动少、高血压、甘油三酯、低密度脂蛋白与 Fg水平呈正相关 ;而高密度脂蛋白与 Fg呈负相关 ;遗传因素对 Fg有一定影响 ;Fg浓度升高是 CHD发病的独立危险因素     

薯蓣皂甙对血脂紊乱治疗的临床研究

目的观察薯蓣皂甙对血脂紊乱患者的临床疗效。方法将106例血脂紊乱患者随机分为两组,对照组仅予饮食控制,治疗组在饮食控制基础上加用薯蓣皂甙片80mg,每日3次,疗程均为12周。于给药前后抽血送检,观察血脂水平变化。结果TC、TG、LDL-C均下降(P<0.05),HDL-C上升(P<0.05),对照组差异无统计学意义(P>0.05)。结论薯蓣皂甙能有效调节脂质代谢紊乱。     

A single nucleotide polymorphism of the adenosine deaminase, RNA-specific gene is associated with the serum triglyceride level, abdominal circumference, and serum adiponectin concentration

Background

Single nucleotide polymorphisms (SNPs) of the adenosine deaminase, RNA-specific (ADAR) gene were reported to be associated with human longevity. There are possibilities that ADAR is associated with major risk factors of atherosclerotic cardiovascular diseases (CVD), such as hypertension, diabetes, dyslipidemia, and obesity.

Objective

To investigate the association between SNPs of the ADAR gene and clinical data associated with major risk factors of atherosclerotic CVD.

Subjects

A total of 1504 general population residents (586 males and 918 females) of two towns, Tanno-cho and Sobestu-cho, in Hokkaido, Japan.

Methods

Clinical data associated with risk factors of atherosclerotic CVD were collected from these study subjects. DNA from peripheral blood and written informed consent were obtained. Three single nucleotide polymorphisms of ADARB1 and ADARB2, which were previously reported to be associated with longevity, were genotyped employing the TaqMan PCR method. The associations between SNPs in ADARB1 and ADARB2 and clinical parameters related to risk factors of atherosclerosis were analyzed.

Results

On uni- and multivariate analyses, rs2805533 in ADARB2 was significantly associated with the abdominal circumference, body mass index, serum triglyceride level, and serum adiponectin level. The subjects with the AA genotype of rs2805533 had a greater abdominal circumference, higher body mass index, higher triglyceride level, and lower adiponectin level than those with AG and GG genotypes.

Conclusion

The SNP in ADARB2 related to longevity is associated with metabolic disorders. This finding suggests that genetic factors modulate human longevity via the regulation of metabolic factors such as abdominal obesity and lipid profiles.     

The effect of thiazolidinediones on adiponectin serum level: a meta-analysis

Background and aims:  Adiponectin is a hormone mainly produced by white adipose tissue. Decreased levels of adiponectin are linked with visceral obesity, insulin resistance states, and cardiovascular diseases. Recently, several studies have pointed out an increase in adiponectin serum levels in subjects undergoing treatment with thiazolidinediones (TZD). The aim of this study is to systematically review the current state of evidence of the effect of TZD on adiponectin serum level with special attention to avoid publication bias.
Materials and methods:  An extensive literature search was performed. M eta A nalysis Version 2.0 computer program was used to calculate statistical differences in means and 95% confidence interval (CI). Publication bias was assessed using different statistical approaches.
Results:  In the meta-analysis including 19 studies the overall standardized mean difference was 0.94 (95% CI, 0.81–1.06) which means that subjects treated with TZDs on average had means of adiponectin concentration that were about 1 standard deviation higher than the comparison groups even after controlling for possible biases.
Conclusions:  The results obtained agree with a moderate increase of serum adiponectin. The results clearly reveal an increase of endogenous serum adiponectin levels by intake of TZDs and may point to a potential new option to manage obesity-related diseases.     

Assessment of genetic effects of polymorphisms in the MCP-1 gene on serum MCP-1 levels and myocardial infarction in Japanese.

BACKGROUND: Recently, the Framingham Heart Study reported that genetic variations in CCL2 influence serum levels of monocyte chemoattractant protein-1 (MCP-1) and the incidence of myocardial infarction (MI). The purpose of the present study was to investigate the possible involvement of CCL2 in the pathogenesis of atherosclerosis and MI in Japanese. METHODS AND RESULTS: Multiple regression analysis indicated that the MCP-1 levels were significantly influenced by various factors including age, body mass index, smoking, alcohol intake, high density lipoprotein-cholesterol, and systolic blood pressure. Moreover, the serum MCP-1 level was significantly correlated with intima - media thickness (p < 0.0001). However, this association disappeared when other clinical confounding factors were included in the analyses. Comprehensive analysis of common polymorphisms of CCL2 in a large community-based population and in subjects with MI found that the A(-2138)T polymorphism affected the serum MCP-1 level in a subgroup of subjects 65 years and older. However, no significant differences in the frequencies of any of the polymorphisms or haplotypes were found between subjects with and without MI. None of the polymorphisms in CCL2 affected carotid atherosclerosis. CONCLUSIONS: The serum MCP-1 level was a good surrogate marker of atherosclerosis in the present study population. Although genetic variations in CCL2 may have some influence on MCP-1 production, their influence does not seem to contribute appreciably to atherosclerosis in Japanese. The present results did not support the recently published findings from the Framingham Heart Study. The discrepancy between the 2 studies may be related to differences in confounding factors that contribute to MCP-1 levels and in the haplotype structure of the 2 populations.     

载脂蛋白A-V对血脂的调节作用

载脂蛋白A-V对血浆脂质特别是甘油三酯的代谢有明显的调节作用,载脂蛋白A-V基因的过度表达可引起血浆甘油三酯水平显著下降,同时可能与高密度脂蛋白的增高及极低密度脂蛋白和胆固醇的下降有关.血脂代谢紊乱与冠心病的发生发展密切相关,故对载脂蛋白A-V调脂机制及转录表达的研究有重要的临床意义.     

Pitavastatin prevents postprandial endothelial dysfunction via reduction of the serum triglyceride level in obese male subjects

Obesity is a well-established risk factor for the development and progression of coronary heart disease. Moreover, endothelial dysfunction is an early event in atherosclerosis and is known to be associated with postprandial hypertriglyceridemia. The purpose of this study was to determine whether a statin might have an effect on postprandial hypertriglyceridemia, and thereby on endothelial function in obese subjects. Twenty-four obese male subjects were recruited for this study. They were randomly assigned to receive pitavastatin (2 mg/day) or placebo for 2 weeks. The oral fat loading test using OFTT cream was performed pre- and post-treatment, in which the lipid profile and flow-mediated dilation (FMD) were assessed before and 4 h after an oral fat load. In the oral fat loading test conducted pretreatment, the oral fat load induced a marked increase of the serum triglyceride (TG) level and decrease in FMD in the pitavastatin and placebo group. In the test conducted post-treatment, the increase in postprandial TG was attenuated (+183 vs. +81 mg/dL, P < 0.001) and decrease in postprandial FMD was completely abolished (−1.1 vs. +0.1%, P < 0.01) by pitavastatin treatment. Moreover, there was a good correlation between the change in postprandial TG and the change in postprandial FMD after the 2 weeks of treatment (r = −0.737, P < 0.001). Pitavastatin might prevent endothelial dysfunction caused by postprandial hypertriglyceridemia within 2 weeks of therapy in obese subjects.     

高三酰甘油血症对大鼠胰腺腺泡细胞的影响

目的 研究高三酰甘油血症对大鼠胰腺腺泡细胞形态和功能的影响.方法 断奶1周雄性SD大鼠(约70 g)随机分为2组,每组10只,分别给予高脂饲料和普通饲料喂养4周.检测血清三酰甘油(TG)、胆固醇(TC)及游离脂肪酸(FFA)水平,HE染色和透射电镜观察胰腺腺泡细胞形态.胶原酶消化法分离大鼠胰腺腺泡细胞,台盼蓝染色测定细胞活力.给予不同浓度胆囊刺激素(CCK-8)刺激胰腺腺泡细胞分泌淀粉酶及乳酸脱氢酶(LDH),测定酶释放率. 结果 高脂饮食饲喂大鼠4周后,血清TG、FFA较正常饮食组明显升高(P ＜ 0.05).高脂饮食组大鼠胰腺腺泡细胞出现空泡样改变及内质网扩张.体外培养6 h后,高脂饮食组胰腺腺泡细胞活力低于对照组(P ＜ 0.05),且各浓度CCK-8引起的淀粉酶和LDH释放均高于正常饮食组(P ＜ 0.05).结论 饮食诱导的高三酰甘油血症可以引起大鼠胰腺腺泡细胞损伤.