感染后与非感染后IBS患者结肠黏膜SP与IL-2、IFN-γ表达的相关性

目的:探讨肠道感染对IBS患者结肠黏膜SP,IL-2,IFN-γ表达的影响.方法:77例IBS患者(PI-IBS组26例,非PI-IBS组51例)及30例对照者,结肠镜下活检降结肠和直肠黏膜标本,采用免疫组化检测其肠黏膜SP与IL-2,IFN-γ的表达情况.结果:PI-IBS患者结肠黏膜SP的表达高于非PI-IBS患者(t=2.321,2.452,2.414,2.520,P<0.05)和对照组(t=3.623,3.722,3.454,3.561,P<0.01);PI-IBS患者结肠黏膜IFN-γ和IL-2阳性率表达高于非PI-IBS患者(χ2=10.010,9.892,9.984,10.152,P<0.05)和对照组(χ2=13.781,13.890,14.012,13.931,P<0.01);IFN-γ和IL-2阳性表达的PI-IBS患者结肠黏膜SP表达高于非PI-IBS患者(t=2.182,2.230,2.194,2.174,P<0.05)和对照组(t=2.202,2.220,2.301,2.252,P<0.05).非PI-IBS患者结肠黏膜IFN-γ和IL-2阳性SP的表达者与对照组比较无明显差异.结论:感染可能通过肠黏膜神经-免疫/炎症系统的改变参与了IBS的发病.     

感染后肠易激综合征与溃疡性结肠炎缓解期患者肠黏膜细胞因子表达的相关性

目的:探讨感染后肠易激综合征(PI-IBS)与溃疡性结肠炎(UC)缓解期患者结肠黏膜细胞因子表达的相关性.方法:PI-IBS组26例,UC组45例及对照组30例,结肠镜下活检降结肠和直肠黏膜标本,采用免疫组化SABC法检测其肠黏膜P物质(SP)与IL-2,IFN-γ的表达情况.结果:PI-IBS组降结肠和直肠黏膜IFN-γ和IL-2阳性率表达、SP强度均值和面积高于对照组(IFN-γ:χ2=13.781,14.012,P＜0.01;IL-2:χ2=13.890,13.931,P＜0.01;SP强度:t=3.623,3.722,P＜0.01;SP面积:t=3.454,3.561,P＜0.01),但与UC组患者无显著差异.降结肠、直肠黏膜IFN-γ,IL-2阳性表达的PI-IBS患者,SP强度均值(t=2.202,2.220,P＜0.05)、面积高于对照组(t=2.301,2.252,P＜0.05),与UC组患者也无显著差异.结论:PI-IBS和UC缓解期患者细胞因子表达无显著差异.从神经-免疫机制上分析认为IBS与炎症性肠病(IBD)之间存在某种相关性,IBS可能是轻微的IBD.     

肠易激综合征患者肠黏膜SP、SPR和D5-HT的变化

目的:观察肠易激综合征(IBS)患者回盲部肠黏膜P物质(SP)、P物质受体(SPR)及5-羟色胺(5-HT)的改变及其相互关系,探讨其在IBS病理、生理机制中可能的作用与临床意义.方法:采用EnVision免疫组织化学染色法对IBD患者回盲部黏膜组织切片的SP与SPR进行染色,5-HT用SP免疫组织化学染色法,用彩色病理图像分析软件及免疫组织化学分析软件进行分析.结果:与对照组比较IBS组SP的阳性强度均值、阳性面积均显著增大,差别有统计学意义(F=6.463,P<0.05),其中IBS-D和IBS-C与对照组比较差别有高度统计学意义(Q=4.231、3.463,均P<0.01);IBS组和对照组相比,回盲部黏膜SPR、5-HT阳性细胞明显增多(均P<0.05),且SPR和5-HT密切相关(r=0.1579,P<0.05).结论:SP、SPR及5-HT相互作用在IBS内脏高敏感的病理生理过程中可能具有重要的作用.     

青岛地区感染后肠易激综合征的临床特点

目的 调查分析青岛地区感染后肠易激综合征(PI-IBS)患者的临床特点.方法 收集门诊1BS患者,采用问卷调查方式,内容包括一般资料、症状和微生态制剂治疗前后的生活质量表.结果 (1)PI-IBS患者中女性患病率是男性的2.2倍,与非感染后肠易激综合征(non-PI-IBS)相近;(2)PI-IBS与non-PI-IBS患者都以脑力劳动者居多;(3)PI-IBS与non-PI-IBS患者的消化系统以外的伴随症状中,以躯体性不适为主的表现(疲劳、头痛、头晕、背痛等)的差异无统计学意义(X<'2>=10.5,P>0.05),而以精神性不适为主的表现(紧张、多疑、抑郁、焦虑等)PI-IBS要明显多于non-PI-IBS,差异有统计学意义(X<'2>=28.7,P<0.05);(4)PI-IBS的肠道菌群失调率明显高于non-PI-IBS患者;(5)PI-IBS患者在双歧三联活菌治疗1个月后,生活质最评分的差异有统计学意义(t=3.8,P<0.01),而non-PI-IBS患者治疗前后的差异无统计学意义(t=1.5,P>0.05).结论 青岛地区PI-IBS与non-PI-IBS的某些临床特点存在差异.     

肠易激综合征精神心理因素、肠黏膜肥大细胞及5-羟色胺的变化

目的:探讨精神心理因素、肠黏膜肥大细胞(MC)及5-羟色胺(5-HT)变化在肠易激综合征(IBS)发病机制中的作用.方法:应用汉密尔顿焦虑量表(HAMA)和汉密尔顿抑郁量表(HAMD)分别对27例IBS患者和27例对照者进行精神心理因素评分.应用免疫组化法检测回盲部肠黏膜MC含量.应用高效液相电化学检测法测定回盲部肠黏膜5-HT含量.应用直线相关分析焦虑抑郁评分、回盲部肠黏膜MC数目和脱颗粒比率、5-HT浓度之间的关系.结果:IBS组焦虑抑郁评分HAMA(18.26±6.23)和HAMD(20.93±6.96)总分均明显高于对照组(9.15±4.91,9.89±5.31),差异有显著性(P＜0.05).IBS组回盲部肠黏膜MC数目、MC脱颗粒比率便秘型(22.1±6.5/HP,35.4%±7.1%)和腹泻型(28.4±7.3/HP和42.3%±10.1%)明显高于对照组(15.6±6.9/HP和24.8%±7.2%),差异有显著性(P＜0.05),其中腹泻型较便秘型明显升高(P＜0.05).IBS组回盲部肠黏膜5-HT浓度便秘型和腹泻型明显高于对照组(2669±920,2628±906 ng/g vs 1893±984 ng/g,P＜0.05),其中便秘型和腹泻型相比无显著性差异(P＞0.05).IBS患者焦虑抑郁评分与回盲部肠黏膜MC数目、MC脱颗粒比率呈正相关性(rHAMA=0.784,0.842,rHAMD=0.711,0.860,P＜0.01),与回盲部肠黏膜5-HT浓度无明显相关性(P＞0.05).IBS患者回盲部肠黏膜5-HT浓度与MC数目、MC脱颗粒比率之间无明显相关性(P＞0.05).结论:IBS精神心理因素异常,回盲部肠黏膜MC含量及活化比例增高,5-HT浓度增高.精神心理因素异常与肠黏膜MC含量增加及肠黏膜MC的脱颗粒有关.     

肠易激综合征患者肠黏膜肥大细胞的光镜和电镜观察

目的 光镜、电镜下观察肠易激综合征(IBS)患者肠黏膜肥大细胞(MC)的改变及其与P物质(SP)免疫反应阳性纤维、P物质受体(SPR)阳性细胞的关系,探讨其在IBS病理生理机制中的可能作用和临床意义。方法 黏膜标本取自19名正常人和22例腹泻型IBS(D-IBS)患者、20例便秘型IBS(C-IBS)患者的回肠末端、回盲部、升结肠、乙状结肠,应用特殊组织化学染色法(甲苯胺蓝改良染色法)和免疫组织化学染色法分别对MC和SP、SPR进行染色,并应用彩色病理图像分析软件及免疫组织化学分析软件进行分析;采用石蜡连续切片及原位包埋法透射电镜观察MC与SP免疫反应阳性神经纤维的关系。结果 IBS患者回肠末端、回盲部、升结肠MC明显增多(P<0.01),乙状结肠MC无明显变化,IBS患者MC存在显著变异;IBS患者肠黏膜SP免疫反应阳性纤维表达增强(P<0.01);SP免疫反应阳性纤维与MC靠近或毗邻,有些存在“膜膜”接触,增强的阳性纤维强度、面积与MC的面积、密度密切相关;部分肥大细胞呈SPR反应阳性。结论 MC与SP免疫反应阳性神经纤维及其相互作用在IBS内脏高敏感的病理生理过程中可能具有重要作用。     

肠易激综合征患者结肠黏膜T细胞、B细胞、神经肽、细胞因子变化及其相关性

目的 探讨肠易激综合征(IBS)患者结肠黏膜T、B淋巴细胞、P物质(SP)、血管活性肠肽(VIP)及IL-1β的表达和相关性.方法 黏膜标本取自21例IBS患者和17例正常人回盲部、乙状结肠.应用流式细胞仪检测结肠黏膜CD3+、CD28+、CDl9+、CD38+表达,放射免疫测定法检测结肠黏膜SP、VIP和IL-1β含量.结果 IBS患者回盲部黏膜CD3+T细胞、CD3+CD28+T细胞均较对照组明显增加(P均《0.01),乙状结肠黏膜两组之间无明显差异(P》0.05);回盲部和乙状结肠CD19+B细胞和CDl9+CD38+B细胞与对照组的差异无统计学意义(P》0.05).IBS患者回盲部黏膜IL-1β、SP和VIP含量均较对照组升高(P《0.01),乙状结肠黏膜SP和VIP含量也均较对照组升高(P《0.01),而乙状结肠黏膜IL-1β变化无统计学意义(P》0.05);IBS患者回盲部IL-1β、VIP含量较乙状结肠明显升高(均P《0.01).回盲部黏膜CD3+28+T细胞分别与IL-1β和VIP存在直线正相关(P《0.58,P《0.01和P《0.81,P《0.01),IL+1β和VIP也存在直线正相关(P《0.75,P《0.01).结论 IBS患者回盲部黏膜表达T细胞增殖和活化,IL-1β、VIP和SP参与IBS的发病,它们之间存在相关性.     

肠易激综合征患者肠黏膜肥大细胞与P物质的相关性

目的　研究肠易激综合征 (IBS)患者肠黏膜肥大细胞 (MC)、P物质 (SP)的变化及其相互关系 ,探讨其在IBS内脏高敏感性中的可能作用和临床意义。方法　黏膜标本取自 2 2例腹泻型IBS患者、2 0例便秘型IBS患者和 19例正常人的回肠末端、回盲部、升结肠、乙状结肠 ,应用甲苯胺蓝改良染色法和免疫组织化学染色法分别对MC和SP、SP受体 (SPR)进行染色 ,并应用彩色病理图像分析软件及免疫组化分析软件进行分析 ;应用放射免疫测定法检测黏膜SP含量 ;采用石蜡连续切片观察MC与SP免疫反应阳性神经纤维、SPR免疫反应阳性细胞的关系 ;采用原位包埋法透射电镜观察MC与SP免疫反应阳性神经纤维的关系。结果　IBS患者回肠末端、回盲部、升结肠MC明显增多 (P <0 0 1) ,乙状结肠MC无明显变化 ;IBS患者MC存在显著变异 ;IBS患者肠黏膜SP免疫反应阳性纤维表达增强 (P <0 0 1) ,其含量较正常对照组显著升高 (P <0 0 1) ;SP免疫反应阳性纤维与MC靠近或毗邻 ,有些存在“膜膜”接触 ,增强的阳性纤维强度、面积与MC的面积、密度密切相关 ;部分MC呈SPR免疫反应阳性。结论　MC、SP及MC与SP免疫反应阳性神经纤维的相互作用在IBS内脏高敏感的病理生理过程中可能具有重要作用     

拉米夫定治疗对慢性乙型肝炎患者干扰素-γ和白细胞介素-4的影响

目的 观察拉米夫定治疗慢性乙型肝炎患者血清IFN-γ和IL-4水平的动态变化.方法 ELISA法分别检测66例慢性乙型肝炎患者在拉米夫定治疗前,治疗后第3、6、9、12个月时的血清IFN-γ和IL-4水平.选取20名健康献血员作为健康对照.治疗前后比较用t检验,计数资料采用非参数秩和检验.结果 HBeAg阳性患者拉米夫定治疗前.完全应答组IFN-γ水平为(21.03±4.44)ng/L,明显高于部分应答组的(13.85±3.92)ng/L及无应答组的(10.63±3.11)ng/L(t=7.56,t=11.87,均P<0.01);以IFN-γ为15.66 ng/L为界,治疗前IFN-γ高水平患者完全应答率明显高于低水平患者(31.0%比8.7%,x2=8.391,P<0.01),无应答率明显低于低水平患者(13.8%比52.2%,x2=4.256,P<0.01).治疗后完全应答组患者IFN-γ/IL-4接近或高于对照组,部分应答组和无应答组低于对照组;HBeAg阴性患者拉米夫定治疗后IFN-γ/IL-4缓慢上升,但未达对照组水平.结论 拉米夫定治疗慢性乙型肝炎可增加IFN-γ释放,抑制IL-4释放,治疗应答程度与治疗后辅助性T淋巴细胞(Th)1/Th2平衡的恢复及治疗前IFN-γ水平有关.     

慢性心力衰竭患者炎性细胞因子的变化及作用

目的探讨致炎细胞因子白细胞介素1(IL-1)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)、γ干扰素(IFN-γ)和抗炎细胞因子白细胞介素10(IL-10)在慢性心力衰竭发生发展中的变化及作用。方法入选心力衰竭患者150例和健康对照50名,采用酶联免疫吸附法检测血清IL-1、IL-6、IL-10、TNF-α和IFN-γ的水平。结果心力衰竭组患者血清IL-1、IL-6、IL-10、TNF-α和IFN-γ的水平显著高于对照组(P<0.05或P<0.01),且随着纽约心功能分级(NYHA)的增加而升高,并与左心室射血分数(LVEF)呈负相关(r=-0.586、-0.454、-0.521、-0.514、-0.502,均为P<0.01),与左心室舒张末容积(LVEDV)呈正相关(r=0.603、0.45、0.542、0.519、0.438,均为P<0.01);IL-1、IL-6、TNF-α、IFN-γ总和与IL-10的比值:NYHAⅢ级和Ⅳ级患者高于对照组(P<0.05或P<0.01),NYHAⅣ级患者高于Ⅲ级和Ⅱ级患者(P<0.05),NYHAⅢ级患者高于Ⅱ级患者(P<0.05),但NYHAⅡ级患者与对照组间差异无统计学意义(P>0.05);冠心病心力衰竭和扩张型心肌病心力衰竭患者的IL-1、IL-6、IL-10、TNF-α和IFN-γ水平比较,差异无统计学意义(P>0.05)。结论心力衰竭患者致炎细胞因子和抗炎细胞因子均增高,但抗炎因子增加相对不足,炎症反应随着心力衰竭程度加重而加重,与心功能状态有相关性,与病因无显著相关性,细胞因子在心力衰竭的发生发展中起着重要的作用。     

肠易激综合征肥大细胞与P物质免疫反应阳性神经纤维的实验研究

目的　探讨肠易激综合征 (IBS)患者回盲部 (ICJ)肥大细胞 (MC)、P物质 (SP)免疫反应阳性神经纤维有无变化及其在IBS中的可能作用和临床意义。方法　黏膜标本取自 2 0名正常人和 31例IBS患者回盲部 ,应用特殊组化染色法 (甲苯胺蓝改良染色法 )和免疫组化染色法分别对MC和SP免疫反应性神经纤维进行染色 ,并应用彩色病理图像分析软件及免疫组化分析软件进行分析 ;电镜观察MC与神经纤维的关系。结果　IBS患者回盲部MC明显增多 (P <0 .0 1 ) ,SP免疫反应阳性纤维表达增强(P <0 .0 1 ) ,两者密切相关 (r =0 .646 7,P <0 .0 1 ) ;SP免疫反应阳性纤维与MC靠近或毗邻 ,神经纤维属无髓纤维。结论　MC与SP免疫反应阳性神经纤维关系密切 ,MC、SP免疫反应阳性神经纤维及其相互作用在IBS的病理生理中具有重要意义 ,并为研究神经内分泌与免疫调节的双向交流提供了形态学基础。     

Distinctive clinical,psychological, and histological features of postinfective irritable bowel syndrome

OBJECTIVE: Irritable bowel syndrome after gastroenteritis is well recognized. Our aim was to determine whether postinfective IBS (PI-IBS) has histological or clinical features that are distinct from those of IBS patients with no history of preceding infection. METHODS: A total of 75 consecutive IBS outpatients and 36 healthy control subjects completed a questionnaire detailing symptoms, mode of onset, and previous psychiatric history. All underwent a full diagnostic workup including rectal biopsy, which included immunostaining and quantification for lamina propria or intraepithelial T lymphocytes, serotonin-containing enterochromaffin (EC), and mast cells. Patients were divided according to onset of symptoms into PI-IBS (n = 23) or non-PI-IBS (n = 52) patients. RESULTS: Diarrhea predominance occurred more frequently in PI-IBS (70%) than in non-PI-IBS (42%) patients (p = 0.03). A history of previous treatment for anxiety or depression was present in 26% of PI-IBS patients compared to 54% of non-PI-IBS (p = 0.02). Biopsy results for all patients were normal using conventional criteria; however, quantification revealed that PI-IBS showed increased EC cells compared to those of non-PI-IBS patients (p = 0.017) and controls (p = 0.02). Lamina propria T lymphocytes were increased in PI-IBS (p = 0.026) and non-PI-IBS (p = 0.011) patients compared to controls. Mast cells were increased in non-PI-IBS patients (p = 0.054) compared to controls. CONCLUSIONS: Individuals with PI-IBS are a clinically distinct subgroup characterized by diarrheal symptoms, less psychiatric illness, and increased serotonin-containing EC cells compared to those with non-PI-IBS.     

肠易激综合征患者结肠黏膜P物质、血管活性肠肽和肥大细胞的变化

背景：肠易激综合征（IBS）的发病机制尚不明确。目的：探讨IBS患者结肠黏膜P物质（SP）、血管活性肠肽（VIP）、肥大细胞（MC）的变化及其在IBS中的可能作用。方法：20例腹泻型IBS患者、22例便秘型IBS患者和18名正常对照者纳入本研究．取回盲部、乙状结肠黏膜行SP、VIP免疫组化染色和MC计数。结果：IBS患者回盲部、乙状结肠黏膜SP、VIP免疫阳性神经纤维较正常对照组增多、增粗，阳性增强（P〈0．05）。IBS患者乙状结肠黏膜SP、VIP免疫阳性神经纤维与回盲部相比无显著差异。IBS患者回盲部黏膜MC计数较正常对照组显著增高（P〈0．01），乙状结肠黏膜MC计数与正常对照组相比无显著差异。IBS患者乙状结肠黏膜MC计数与正常对照组相比，显著低于回盲部（P〈0．01）。结论：SP、VIP和MC在IBS的发病中起有一定作用。     

Abnormalities of 5-hydroxytryptamine metabolism in irritable bowel syndrome.

BACKGROUND & AIMS: 5-hydroxytryptamine-3 (5-HT 3 ) receptor antagonists improve symptoms in patients with diarrhea-predominant irritable bowel syndrome (D-IBS), 5-HT 4 agonists help those with constipation-predominant IBS (C-IBS). These data suggest excess or deficiency in 5-HT in D-IBS or C-IBS, respectively. Mucosal 5-HT-containing enterochromaffin cells (EC) are increased in postinfectious IBS (PI-IBS). Our aim was to define the postprandial release of 5-HT in PI-IBS and C-IBS patients and to relate this to mucosal 5-HT turnover. METHODS: Fifteen PI-IBS patients with diarrhea-predominant symptoms, 15 C-IBS patients, and 15 healthy controls underwent serial (platelet-poor) plasma 5-HT measurement for 3 hours after a standard 520-kcal meal. Rectal biopsy specimens were assayed for 5-HT and its metabolite 5-hydroxindoleacetic acid (5-HIAA). Colonic transit was measured using radio-opaque markers. RESULTS: Colonic transit was prolonged in C-IBS patients (mean +/- SEM) (49.4 +/- 3.8 h) compared with PI-IBS (26.7 +/- 4.5) and control patients (34.1 +/- 4.5) ( P < .02). Release of 5-HT assessed by area under the curve (AUC) of platelet-poor plasma 5-HT from 0 to 180 minutes postprandially was significantly lower in C-IBS patients (2593 +/- 309 mmol/L . min) compared with P-IBS (5623 +/- 721) and control patients (4822 +/- 598) ( P < .001). PI-IBS patients showed significantly higher peak postprandial plasma 5-HT values (median, range) (71.7, 43.4-125.3) ng/L compared with C-IBS patients (31.2, 15.2-40.5) and control patients (43.6, 26.7-50.1) ( P < .01). Mucosal 5-HT turnover as assessed by mucosal 5-HIAA/5-HT ratio was decreased in both C-IBS and PI-IBS patients, .14 (.01-.6) and .21 (.02-2.5), respectively, compared with control patients 1.12 (.17-3.1) ( P < .002). CONCLUSIONS: C-IBS patients show impaired postprandial 5-HT release whereas PI-IBS patients have higher peak levels, abnormalities that may be related to their different symptoms.     

肠易激综合征患者结肠黏膜辣椒素受体、P物质和肥大细胞变化的研究

背景:腹痛是一般人群中最常见的肠道症状,肠易激综合征(IBS)患者的腹痛症状更为严重、频繁,然而其发生机制尚不明确。目的:分析IBS患者结肠黏膜中与痛觉和内脏高敏感相关的辣椒素受体VR1、P物质(SP)和肥大细胞(MC)的变化,探讨IBS患者内脏高敏感和腹痛的发生机制。方法:39例IBS患者[腹泻型IBS(IBS-D)21例,便秘型IBS(IBS-C)18例]和18名健康人纳入研究。受检者于结肠镜检查时在回盲部和乙状结肠取活检,行VR1、SP免疫组化染色和MC改良甲苯胺蓝染色。结肠镜检查前采用视觉模拟评分法行疼痛评分。结果:IBS患者乙状结肠VR1、回盲部和乙状结肠SP免疫反应阳性细胞以及回盲部MC数量显著多于正常对照组(P0.01),IBS-D与IBS-C组间则无明显差异。IBS-D和IBS-C患者的腹痛评分均与VR1呈正相关(r=0.553,P=0.009;r=0.592,P=0.010)。结论:IBS患者结肠黏膜中VR1、SP免疫反应阳性细胞和MC数量显著增多,VR1与腹痛评分呈正相关,三者可能参与了IBS患者内脏高敏感和腹痛的发生机制。     

P物质和降钙素基因相关肽及炎症因子在非糜烂性食管炎发生中的作用研究

ObjectiveTo explore the mechanism of substance P (SP), calcitonin gene-related peptide (CGRP) and inflammatory cytokines in the development of non erosive reflux disease. MethodsA total of 25 NERD patients and 10 normal controls were enrolled. All the subjects underwent GERDQ score, 24 h pH monitoring, high resolution esophageal manometry monitoring and endoscopic removal of the local mucosa at 3 cm of the esophageal dentate line as specimens. The expression of SP, CGRP and inflammatory cytokines (IL-1β, IL-6, IFN-γ, TNF-α) was detected by HE staining and RT-PCR. Results24 h esophageal pH monitoring showed that the number of weak acid reflux (4＜pH＜7), acid reflux (pH≤4), acid reflux (%) in proximal esophagus and DeMeester score in NERD group were significantly higher than in control group the t values were -2.365, -2.145, -2.782 and -2.776, and the P values were 0.025, 0.021, 0.021 and 0.017, respectively (P＜0.05). H&E staining showed that there were obvious neutrophil infiltration and pre-inflammatory reaction in esophageal mucosa of NERD group, and the score of inflammation injury was significantly higher than that of control group,the P value were 0.003 (P＜0.01). The relative expression of SP and CGRP in NERD group was significantly higher than that in control group, the P values were 0.0046,0.002, respectively (P＜0.01). In NERD group, the relative expressions of IL-1β, IL-6, IFN-γ and TNF-α in esophageal mucosa were significantly increased,P values were 0.0034, 0.0043, 0.004 and 0.0028, respectively (P＜0.01). Pearson correlation analysis showed that the acid reflux score (DeMeester) in NERD group was positively correlated with the expression of SP, CGRP and inflammatory factors (IL-1β, IL-6, IFN-γ, TNF-α) respectively. The P values 0.003, 0.000, 0.002, 0.005, 0.004 and 0.000, respectively (P＜0.01), the correlation coefficients were r=0.678, 0.686, 0.90, 0.482, 0.374 and 0.415, respectively. ConclusionThe expression of SP, CGRP and inflammatory cytokines in NERD patients increased significantly, which may be closely related to the increase of esophageal acid sensitivity caused by acid reflux and the occurrence of inflammation.