臭灵丹对幽门螺杆菌的体外抑菌实验研究

目的 探讨臭灵丹对幽门螺杆菌（H．pylon）甲硝唑耐药株及敏感株的体外抑制作用。方法 琼脂稀释法检测H．pylon甲硝唑耐药株和敏感株对臭灵丹水煎剂的最低抑菌浓度（MIC），并计算MIC50、MIC75、MIC90。结果 臭灵丹对H．pylon的MIC值范围为3．125～200mg／ml，H．pylon甲硝唑耐药株和敏感株对臭灵丹水煎剂的敏感性无统计学差异（相对中位数潜力1．153，95％的可信区间为0．84～1．602），臭灵丹对H．pylon甲硝唑耐药株和敏感株的MIC50分别为21．2mg／ml和18．8mg／ml，MIC75，分别为42．4mg／ml和36．9mg／ml，MIC90分别为779mg／ml和67．8mg／ml。结论 臭灵丹有良好的抑菌效果，H．pylon甲硝唑耐药株及敏感株都对其敏感。     

石榴皮对幽门螺杆菌的体外抑菌实验研究

目的探讨石榴皮对幽门螺杆菌(H.pylori)甲硝唑耐药株及敏感株的体外抑制作用.方法琼脂稀释法检测H.pylori甲硝唑耐药株和敏感株对石榴皮水煎剂的最低抑菌浓度(M IC),并计算M IC50、M IC75、M IC90.结果石榴皮对H.pylori的M IC值范围为7.8 125~500 mg/mL;石榴皮对H.pylori甲硝唑耐药株和敏感株的M IC50分别为29.9 mg/mL和28.0 mg/mL,M IC75分别为65.1 mg/mL和59.1 mg/mL,M IC90分别为131.1 mg/mL和115.9 mg/mL;H.pylori甲硝唑耐药株和敏感株对石榴皮水煎剂的敏感性无统计学差异(相对中位数潜力1.072,95%的可信区间为0.763~1.513).结论石榴皮有良好的抑菌效果,H.pylori甲硝唑耐药株及敏感株都对其敏感.     

生姜、苦瓜、木瓜对幽门螺杆菌甲硝唑耐药株及敏感株的体外实验研究

目的:探讨生姜、苦瓜、木瓜对幽门螺杆菌(H.pylori)甲硝唑耐药株及敏感株的体外抑制作用.方法:(1)制备苦瓜、生姜、木瓜水提液;(2)琼脂稀释法检测苦瓜水提液、生姜水提液、木瓜水提液对H.pylori甲硝唑耐药株和敏感株的最低抑菌浓度(MIC),并计算MIC50、MIC75、MIC90.结果:苦瓜对H.pylori的MIC值范围为0.0048828～0.625g/ml,H.pylorj甲硝唑耐药株和敏感株对苦瓜水提液的敏感性无统计学差异;生姜对H.pylori的MIC值范围为0.01953125～1.25g/ml,H.pylori甲硝唑耐药株和敏感株对生姜水提液的敏感性无统计学差异;木瓜对H.pylori的MIC值范围为0.0136719～1.75g/ml,H.pylori甲硝唑耐药株和敏感株对木瓜水提液的敏感性无统计学差异.结论:苦瓜、生姜、木瓜水提液在体外各自对H.pylori甲硝唑耐药株及敏感株均有良好的抑菌效果.     

中药苦豆子体外抗幽门螺杆菌的实验研究

目的观察苦豆子对幽门螺杆菌(Helicobacter pylori,H.pylori)的体外抑菌作用。方法采用煎煮法与超声波浸提法获取苦豆子不同提取物;纸片扩散法粗测抑菌浓度,琼脂稀释法测定两种提取物的最小抑菌浓度(MIC)。结果苦豆子水煎剂对H.pylori的MIC为100mg/ml,苦豆子乙醇提取物对H.pylori的MIC为25mg/ml。结论两种苦豆子提取物均有较好的体外抗H.pylori作用,苦豆子乙醇提取物对H.pylori的抑菌效果优于水煎剂。     

幽门螺杆菌vaeA cagA基因亚型与抗生素耐药的关系

[仇军文,胡家露,吴开春,彭道荣,施炳龙,季万胜,樊代明.世界华人消化杂志,2001;9(7):739-742] 目的研究幽门螺杆菌(H.pylori)cagA vacA基因亚型及其与抗生素耐药的关系.方法用琼脂稀释法测定50例H.pylori 临床分离株对克拉霉素,阿莫西林,替硝唑,甲硝唑的最低抑菌浓度(MIC),并计算MIC50和MIC90值.用多重PCR鉴定H.pylori菌株的vacA(sla,slb,s2,ml,m2)及cagA的型别.结果克拉霉素,阿莫西林,替硝唑,甲硝唑对H.pylori的MIC52和MIC90分别为:＜0.01 6 mg@L-1,0.016 mg@L1;0.016 mg@L-1,0.047 mg@L-1;16.000 mg@L-1,52.000 mg@L :25.140 mg@L -1 ,76.800 mg@L-1.H.pylori菌株对克拉霉素,阿莫西林,替硝唑,甲硝唑的耐药率分别为0%,0%,20%和38%.在对替硝唑敏感的H.pylori菌株中,vacA sla,slb和s2型分别占50.0%,42.5%和7.5%;vacA ml和m2型占60.0%和40.0%;vacA sla/ml,slb/ml,sla/m2,slb/m2和s2/m2型分别占32.5%,27.5%,17.5%,15.0%和12.5%;cagA-和cagA型为87.5%和12.5%.在对替硝唑敏感的H.pylori菌株中,vacA sla,slb和s2型分别占50.0%,40.0%和10.0:vaccAml和m2型占40.0%和60.0%;vacA sla/ml,slb/ml,sla/m2,slb/m2和s2/m2型分别占20.0%.,20.0%,30.0%.20.0%和10.0%;cagA+和cagA-型为80.0%和20.0%.结论 H.pylori菌株对甲硝唑的耐药率明显高于H.pylori 对克拉霉素,阿莫西林,替硝唑的耐药率,不同cagA vacA基因亚型的H.pylori菌株对替硝唑的耐药率无显著差异. (潘伯荣)     

不同中药提取物对幽门螺杆菌耐药菌株体外抗菌活性研究

目的探索不同中药提取物对幽门螺杆菌(helicobter pylori,H.pylori)耐药菌株是否具有体外抗菌活性,为中药治疗H.pylori耐药菌株提供依据。方法采用琼脂稀释法分别检测不同中药乙醇提取物对H.pylori标准菌株和临床耐药菌株的最小抑菌浓度(MIC),分别计算其MIC50和MIC90。结果中药提取物大黄、黄连、黄芩、败酱草、延胡索的MIC50和MIC90分别为:32,64,32,64,128,256,>512,>512,>512,>512 mg/L。结论大黄及黄连提取物对H.pylori临床耐药菌株具有较明显的体外抑菌作用;黄芩提取物对H.pylori临床耐药菌株具较弱的体外抑菌作用;败酱草、延胡索提取物对于H.pylori临床耐药菌株无明显体外抑菌作用。     

乌药成分异乌药内酯抗H.pylori及其抗菌谱的筛选

目的 筛选异乌药内酯的抗菌谱.方法 微量稀释法检测异乌药内酯对不同H.pylori菌株及非H.pylori菌株的最小抑菌浓度(MIC).结果 异乌药内酯对敏感和耐药H.pylori菌株的MIC均为8～16μg/ml;异乌药内酯对金黄色葡萄球菌等非H.pylori菌株的MIC均>128μg/ml.结论 异乌药内酯对H.p...     

南宁地区解脲支原体对4种抗菌药物敏感性分析

目的:为了了解南宁地区Uu的耐药情况,对本地区支原体耐药情况进行监测,指导临床用药.方法:对南宁地区NGU患者分离出的35株Uu,采用肉汤倍比稀释法测定4种抗生素的MIC值.结果:克拉霉素的MIC≤1μg/ml有17株Uu(71%),MIC50、MIC90分别为1μg/ml、16μg/ml;阿奇霉素的MIC≤4μg/ml有17株Uu(71%),MIC50、MIC90分别为4μg/ml、16μg/ml.美满霉素的MIC≤4μg/m1有11株Uu(占45%),MIC50、MIC90分别为8μg/ml、32μg/ml.氟罗沙星的MIC≤2μg/ml仅有6株Uu(25%)MIC≥8μg/ml的有23株Uu,MIC50、MIC90分别为8μg/ml、16μg/ml.结论:南宁地区的Uu对大环内酯类药物较为敏感,其中克拉霉素优于阿奇霉素.四环素类药物的敏感性较低,喹诺酮类药的敏感性最差.对四环素类药物高度耐药者,71%菌株对其它药物的MIC值较低(17/24).提示临床应警惕三类药物均耐药的情况.     

比较Epsilometer试验法和琼脂稀释法检测幽门螺杆菌对甲硝唑的敏感性

目的：以琼脂稀释法为金标准，评价Epsilometer试验(Epsilometer test, E-test)法检测幽门螺杆菌(Helicobacter pylori,H.pylori)对甲硝唑敏感性的一致性。方法：纳入2018年8月至2020年7月因消化不良症状就诊于北京大学第三医院行胃镜检查的H.pylori感染初治患者，取胃黏膜组织活检行H.pylori培养，分别采用E-test法和琼脂稀释法检测H.pylori对甲硝唑的敏感性，比较两种方法检测结果的一致性和相关性。结果：成功培养105株H.pylori,将最小抑菌浓度≥8 mg/L定义为耐药。琼脂稀释法检测甲硝唑耐药菌株68株，耐药率64.8%,E-test法检测耐药菌株66株，耐药率62.9%,其中，琼脂稀释法和E-test法检测均为耐药的菌株66株，均为敏感的菌株37株，两种方法的一致率为98.1%。2例菌株被琼脂稀释法评价为耐药，而E-test法评价为敏感，非常严重错误率为1.9%。没有菌株被琼脂稀释法评价为敏感，而E-test法评价为耐药(严重错误率为0%)。以琼脂稀释法为金标准，E-test法检测甲硝唑耐药的灵敏度为...     

苦豆子生物碱对幽门螺杆菌耐药株的体外抑菌研究

目的探讨中药苦豆子总碱、苦参碱、苦参素、槐定碱对幽门螺杆菌(Helicobacter pylori,Hp)临床耐药株的体外抑菌作用。方法收集上消化道疾病患者胃黏膜标本,分离、鉴定出42株Hp临床菌株,采用E-test方法检测其对甲硝唑、克拉霉素、阿莫西林的耐药状况,共筛选出27株临床耐药株;采用液体倍比稀释法测定4种苦豆子生物碱对27株临床耐药株的体外抑菌效果。结果 42株临床分离的Hp菌株对甲硝唑、克拉霉素、阿莫西林的耐药率分别为64.3%、14.3%和0。苦豆子总碱、苦参碱、苦参素及槐定碱的MIC50分别为16、64、64、128 mg/ml。结论 Hp菌株对甲硝唑耐药率高,故甲硝唑不应作为根除Hp感染的一线药物。苦豆子总碱在体外抗Hp效果较好,有可能成为治疗Hp感染的一种可选药物。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.