沙眼衣原体、解脲支原体、淋病奈瑟氏菌感染状况分析

目的　探讨本地STD主要病原体的感染状况。方法　采用PCR方法检测我院门诊病人尿道和生殖道标本中沙眼衣原体 (CT)、解脲支原体 (UU)、淋病奈瑟氏菌 (Ng)。结果　男性患者CT、UU和Ng分别为 8 4 % ,9 0 %和2 2 0 % ,女性患者CT、UU和Ng感染率感染率分别为 8 2 % ,17 0 %和 8 3%。结论　本地区男女的STD主要病原体的感染谱不同 ,男性以Ng感染为主 ,女性以UU感染为主 ,UU已取代Ng成为女性STD首要病原体。     

1198株淋病奈瑟氏菌的药物敏感性分析

淋病奈瑟氏菌 (ＮＧ)引起的淋病是目前世界上一种常见的性传播疾病 ,是我国目前流行的性传播疾病中发病率最高的一种 ,急性淋病如未采取有效的药物治疗 ,将导致严重后果。本文就性传播尿道炎及阴道炎患者的淋病奈瑟氏菌及其药物敏感性 (简称药敏 )进行探讨 ,现报告如下。1　材料和方法1 1　材料1 1 1　标本来源　 1 995年 8月～ 1 999年 1 0月我院门诊住院的尿道炎及阴道炎患者 ,年龄 6～ 75岁 ,其中女性436 6例 ,男性 82 9例。1 1 2　培养基　采用淋球菌选择性培养基Ｔ -Ｍ (英国ＯＸＯＬＤ公司提供 )。1 1 3　药敏纸片　青…     

339例患者淋病奈瑟菌检查结果分析

淋病是由淋病条瑟菌引起的泌尿生殖器化脓性疾病，主要经过性接触传染。近年来，由于改革开放和人民生活水平的不断提高，性病的发病率明显呈上升趋势，其中尤以淋病发病率最高。笔者对我院2001年1月-2005年1月间来院就诊的339例患者涂片检查情况进行了统计分析，现报告如下。     

上饶地区淋病奈瑟菌临床耐药性分析

目的了解上饶地区近年淋病奈瑟菌（NG）的耐药特点及产β-内酰胺酶淋病奈瑟菌（PPNG）的流行状况,为本地区淋病治疗合理选用抗生素提供指导。方法采用K-B琼脂扩散法,测定临床分离鉴定的NG对8种常用抗生素的敏感性;应用硝基噻吩显色法检测PPNG。结果 116株NG对8种抗生素的耐药率从高到低依次为：氧氟沙星（91.4%）、四环素（83.6%）、氨苄青霉素（55.2%）、头孢噻吩（18.9%）、头孢呋辛（12.0%）、头孢噻肟（7.8%）、头孢曲松（6.9%）和大观霉素（4.3%）;检测出PPNG54株（46.6%）。结论氧氟沙星、四环素和氨苄青霉素不宜作为本地区淋病治疗药物,大观霉素和第三代头孢菌素可作为首选药物,但要加强耐药性检测。     

生化酶法检测淋病球菌感染的评价

近年来,性病发病逐渐升高。1996年院皮肤科性病专科门诊中,发现淋病奈瑟氏菌(NG)感染高危者183例进行NG培养、涂片和生化酶法检查,以NG菌培养阳性者89例作为确诊组,以49例女职工妇科健康普查作对照组,高危病人183例的生殖道淋病奈瑟氏菌(NG)培养、涂片和生化酶法检查的阳性率分别为48.6％、57.9％和96.2％。研究结果表明NG细菌培养虽是NG感染的“金标准”,但菌株营养要求高,对理化因素抵抗力低,影响阳性率;涂片易漏诊,尤其对女性患者,不能确切反应淋病的发病情况。人们一直在努力寻找更敏感、特异、简便的方法。本文选用了美国SYNTRON公司研制的生化酶法试剂盒,敏感度达到94.4％,远远高出培养和涂片法,并特异性强,达到98.0％,操作简便、快速,不需要特殊设备,能适用于各级医疗机构,便于推广应用,作为NG感染的初筛及NG培养、涂片的补充检测,能减少漏诊,防止性病的传播,具有很大的实用价值。     

淋球菌单克隆抗体Ng4和Ng18于淋病实验病诊断的可行性研究

通过ＥＬＩＳＡ抑制试验和流式细胞仪测定，对可能用于淋病实验室诊断的单克隆抗体进行了评价，实验结果显示出良好的亲和性，在各血清型淋球菌标准株和临床株的检测中与国外同类产品进行了比较，证明了其作为诊断试剂的可行性。     

快速染色法检查淋病奈瑟氏菌

革兰氏染色法是涂片检查淋病奈瑟氏菌的传统方法,但阳性检出率低。笔者采用天青美蓝快速染色检查,取得满意效果,现介绍如下。     

免疫荧光检测阴道加德纳氏菌

加德纳氏菌（GV）是细茵性阴道病（BV）的主要病原体，可经性接触传播，由于该病菌生长条件苛刻，影响了该菌培养的开展。为此，我们应用免疫荧光和培养方法进行比较，现将结果报告如下。      

淋球菌单克隆抗体Ng4和Ng18用于淋病实验室诊断的可行性研究

通过ＥＬＩＳＡ抑制试验和流式细胞仪测定，对可能用于淋病实验室诊断的单克隆抗体进行了评价，实验结果显示出良好的亲和性，在各血清型淋球菌标准株和临床株的检测中与国外同类产品进行了比较，证明了其作为诊断试剂的可行性。     

SPA协同凝集诊断淋病奈瑟氏菌

淋球菌为淋病的病原菌,临床常依靠细菌培养来帮助诊断。传统的检查方法是先从初代培养物中挑选出可疑菌落,通过传代增殖,再依靠多种生化反应鉴定。这种方法操作繁琐,需时间较长。应用免疫学的方法检查淋球菌,国外已有报道。我们参照其方法,应用标准淋球菌免疫家兔,制备抗血清,用此血清致敏含A蛋白的金黄色葡萄球菌(SPA)制成抗淋球菌—SPA诊断试     

亚甲蓝/光化学灭活病毒法对血浆中抗体活性的影响

目的：研究亚甲蓝／光化学灭活病毒技术对人血浆中抗体活性的影响。方法：将不同浓度的亚甲蓝分别加入含有特种抗体的血浆中，经JY—Ⅰ型血浆病毒灭活仪处理后取样，用ELISA等方法检测血浆中的抗体活性。结果：在光照度40000lx条件下，经1μmol／L亚甲蓝作用30min，人血浆中的抗体活性未见明显改变，但随着亚甲蓝浓度的升高和光照时间的延长，血浆中的抗体活性略有下降。结论：本研究的亚甲蓝／光化学灭活病毒技术对血浆中的抗体活性影响较小，可用该技术对康复期的SARS患者血浆进行病毒灭活处理，保证该血浆临床应用的安全性。     

Study on a method to improve T1 image contrast by the subtraction technique for 3.0 T brain examination

The purpose of this study is to describe the enhanced gray/white brain matter delineation achieved by a novel subtraction technique. We subtracted T2-weighted from T1-weighted images. The resulting images were analyzed for contrast-to-noise ratio and reader preference. Our results show superior outcomes for the subtracted images. This improvement could be used to better identify tumor enhancement or cortical dysplasia.     

Left atrial dimension on the frontal thoracic radiograph: a method for assessing left atrial enlargement.

Left atrium size on the frontal radiograph was assessed by measuring the distance from the middle of the "double density" to the left main bronchus in 148 normal patients and 48 consecutive patients with echocardiographic evidence of left atrial enlargement. The measurement was less than 7.0 cm in 96% of normal patients and greater than 7.0 cm in 90% of patients with left atrial enlargement. This measurement is more easily obtainable than measurements and signs requiring a cardiac series, and is usually readily available for sequential evaluations of left atrial size. It is a less reliable gauge of left atrial size in children.     

1H decoupling for in vivo (19)F MRS studies using the time-share modulation method on a clinical 1.5 T NMR system.

Time-share modulation has been implemented successfully on a clinical 1.5 T nuclear magnetic resonance (NMR) system to perform in vivo (19)F[-(1)H] decoupling. It uses commercially available radiofrequency (RF) filters, a transistor-transistor logic (TTL)-controlled attenuator, and a double-resonant RF surface coil suitable for (19)F magnetic resonance spectroscopy (MRS) studies in vivo. This decoupling scheme gives a threefold improvement in signal-to-noise ratio performance compared with the conventional WALTZ-4 decoupling scheme, when significant interaction exists between the decoupler and the receiver. It can also eliminate receiver saturation (i.e., saturation due to coupling of the strong proton decoupling pulse to the (19)F coil) without the need for a high-isolation coil system, thus allowing flexibility in coil geometry. This method has been demonstrated in vivo in patients receiving 5-fluorouracil chemotherapy. Magn Reson Med 44:5-9, 2000.     

A practical approach to the hepatobiliary kinetics of 99mTc-HIDA. Clinical validation of the method and a preliminary report on its use for parametric imaging

^99mTc-diethyl-HIDA cholescintigraphy was performed on 24 patients with histologically proven liver disease and on 10 normal adult subjects. Liver mass transport of HIDA was interpreted with the aid of a probabilistic model, assuming that the tracer particles undergo mixed random walks with drifts. The radiohepatograms were thus fitted with a gamma-variate function and the fitting parameters were evaluated as estimates of the severity of the disease. These parameters, together with the transit times, were also used to generate parametric images of liver function. The advantages of this approach are discussed and the following conclusions are drawn: (1) The gamma-fit is highly satisfactory with any type of experimental curve (0.98<r<1). (2) The parameters derived from the gamma-fit make it possible to objectively assess the extent of liver functional impairment. (3) Parametric imaging of the liver mass transport of HIDA is easily implemented, but still more experience is needed to assess its impact on patient care.