老年性白内障血清、晶体及房水微量元素的测定

检测了部分老年性白内障房水,晶体及不同时期血清的13～33种元素的含量,结果表明:1.白内障晶体中Zn,K,As,P,Rb,Sb,Bi含量较透明晶体明显降低。Ca,Fe,Na,Li,Cr,Ba含量较透明晶体明显升高。2.随着白内障的发展,血清中Zn,Mg,Ti,Li,Si,Sn含量逐渐降低,白内障成熟时,血清中除Mg以外余者含量均降到最低,而Al的含量却逐渐升至最高,白内障成熟时Al的含量达到最高。3.白内障房水中Ca、K含量较透明晶体明显升高。上述结果表明,微量元素与白内障密切相关,可能是白内障的致病因素之一。     

不同剂量地塞米松对中枢、神经-肌接头和肌疲劳的影响

目的 :研究不同剂量地塞米松对中枢、神经 -肌接头和肌疲劳的影响。方法 :用计算机MS30 2生物信号分析系统记录蟾蜍坐骨神经干动作电位和腓肠肌收缩张力 ,检测中枢、神经 -肌接头和肌肉出现疲劳的时间。结果 :① 1mg kg及以下较小剂量的地塞米松能延缓中枢、神经 -肌接头和肌的疲劳 ,与对照组比较 ,差异具有显著性 (P <0 .0 5 )。② 2mg kg及以上较大剂量的地塞米松则有加速上述部位疲劳 ,降低肌耐力的作用 ,与对照组比较 ,差异亦具有显著性 (P <0 .0 5 )。结论 :不同剂量的地塞米松对中枢、神经 -肌接头和肌的影响不一致。     

论脏腑之气皆有升降出入

任何脏腑之气的运动形式,皆非囿于升或者降一方面,而是包括升降出入四个方面。脏腑之气的这一运动规律,既可体现于整体,也可体现于局部;既可体现于脏腑之间,也可体现于一个脏或一个腑。     

沙苑子总黄酮对运动训练大鼠血液中某些生化指标影响的实验研究

目的测定大鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、肌酸激酶(CK)的活性和血液中血尿素氮(BUN)、血糖(GLUC)、血红蛋白(Hb)的含量,考察沙苑子对运动训练大鼠重要脏器的保护作用。方法本实验以成年雄性Sprague-Daw-ley(SD)大鼠为研究对象,建立力竭跑台运动训练模型。结果力竭运动导致大鼠血清ALT、AST、LDH、CK活性和血BUN值较安静组大鼠显著性升高(P<0.01),G luc、Hb含量较安静组大鼠显著性降低(P<0.01)。服用沙苑子组的大鼠血清ALT、AST、LDH、CK活性和血BUN值极显著低于运动力竭组,G luc、Hb含量显著高于运动力竭组。结论沙苑子总黄酮可保护大鼠重要器官免受运动损伤,有利于维持运动过程中血红蛋白和血糖水平的稳定,明显延缓大鼠运动性疲劳的发生,使大鼠运动能力明显提高。     

内蒙古锡盟地区蒙古族人群VWA、FGA、PENTA E基因座的群体遗传学研究

目的：了解内蒙古锡盟地区蒙古族人群VWA、FGA、PENTAE三个STR位点的遗传多态性分布，获得相应多态位点的群体遗传学数据。方法：采用多重PCR扩增技术和聚丙烯酞胺凝胶电泳方法对263名蒙古族个体进行了研究。结果：VWA、FGA、PENTAE三个位点分别检出9；10、22个等位基因，多态性分布均符合Hardy-Weinberg平衡定律。杂合度(H)分别为0．8l15、0．8487、0．9146，多态信息量(PIC)分别为0．7825、0．8298、0．9064。结合：上述三个位点均具有较高的杂合度和多态信息量，是较理想的遗传标记系统。同时揭示了蒙古族人群三个STR位点的遗传多态性分布，为人类群体遗传研究提供了数据。     

REASSESSMENT OF DEFIBRASE IN TREATMENT OF ACUTE CEREBRAL INFARCTION： A MULTICENTER, RANDOMIZED, DOUBLE-BLIND,PLACEBO-CONTROLLED TRIAL

Objoctive To evaluate the efficacy and safety of defibrase in patients with acute cerebral infarction by a large sample, multicenter, randomized, double-blind, placebo-controlled clinical trial. Mothods Patients with acute cerebral infarction within 12 hours of stroke onset were randomly assigned to receive either an initial intravenous infusion of defibrase 15 U plus normal saline 250 mL or 250 mL of normal saline only. Subsequent infusions of defibrase 5 U or placebo （normal saline） were given on the 3rd, 5th, 7th, and 9th day, respectively. Both groups received standard care of acute cerebral infarction. The primary efficacy outcome was functional status （Barthel Index） at 3 months after treatment. Safety outcome were bleeding events and mortality rate. Secondary outcome included Chinese Stroke Scale （CSS） score at 14 days and recurrence rate of stroke at 1 year. A total of 1053 patients were enrolled at 46 centers from September 2001 to July 2003, and 527 patients were randomly assigned to receive defibrase and 526 to receive placebo. A similar proportion of patients in both groups completed a full course of treatment. There was a significantly greater proportion of favorable functional status （Barthel Index 1≥95） in defibrase group than in placebo group at 3 months （52.2% vs. 42.8%, P 〈 0.01）, and the proportion of dependent functional status （Barthel Index ≤60） was a little lower in defibrase group compared with placebo group （27.7% vs. 32.4%）. These differences were more obvious among patients who were treated within 6 hours of stroke onset. Patients in defibrase group had better improvement with respect to CSS score than those in placebo group at 14 days （P 〈 0.05）. Recurrence rate of stroke at 1 year was lower in the defibrase group compared with placebo group （6.2% vs. 10.1%, P = 0.053）. Patients in defibrase group had higher risk of extracranial bleeding events （4.7% vs. 1.5%, P 〈 0.01 ） and a tendency of higher risk of symptomatic intracranial hemorrhage. The hemorrhage incidence was higher in patients with fibrinogen level 〈 130 mg/dL than ≥ 130 mg/dL （10.6% vs. 3.8%, P 〈 0.05）. Mortality rate at 3 months were slightly higher in defibrase group than placebo group （5.9% vs. 4.2%）. Conclusions The defibrase is effective to improve neurological function and function of daily living for patients with acute cerebral infarction within 12 hours of symptom onset. The efficacy was even better for acute cerebral infarction within 6 hours of onset. The increased risks of intra- and extracranial hemorrhage during defibrase administration were related to the plasma fibrinogen level.     

第一鳃裂瘘(附4例报告)

本文报告4例第一鳃裂瘘讨论了胚胎发生学、分型、诊断及治疗。认为两型分法对手术治疗有指导意义。凡耳后或下颌角附近的持续性瘘管都应考虑本病的可能，尤其是伴有耳道流脓而听力及鼓膜正常者，应考虑本病。手术彻底切除是唯一有效的治疗方法。     

内毒素、TNF-α、IL-6在慢性肝炎中的作用研究

目的研究内毒素血症与慢性肝炎的关系,探讨内毒素在慢性肝炎中的作用机理.方法收集如下血标本正常健康献血员30例,慢性乙型肝炎患者30例,慢性深度黄疸乙型肝炎患者60例.采血当日送检功能.分别用鲎实验改良基质法进行内毒素定量测定,放射免疫方法(RIA)测定肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6).结果内素素、TNF-α、IL-6和总胆红素(TBIL)水平在三组患者中依次升高,各组两两比较,其差异有显著性意义(P＜0.01);四项指标进行直线相关性分析,各指标之间呈正相关关系(P＜0.01).结论内素素血症与肝脏损害的严重程度有关;内毒素可能主要是通过引起以下TNF-α为主的细胞因子水平的升高发挥肝脏损害作用的.     

银屑病患者的ANA,CIC,Ig及C3检测

作者采用间接免疫荧光技术、PEG沉淀法及单向琼脂扩散试验对50例银屑病患者血循环免疫复合物(CIC)、抗核抗体(ANA)、免疫球蛋白(Ig)及补体(C_3)含量进行观测。结果表明患者CIC、ANA、Ig含量增多,而C_3含量降低,与对照组相比(P<0.01),差异有高度显著性。故认为银屑病的发生与免疫病理损伤有关。     

二亚硝基哌嗪对大鼠鼻咽鳞状上皮更新的影响

本文用放射自显影术对正常大鼠以及二亚硝基哌嗪(简称二亚)处理鼠诱癌过程中鼻咽鳞状上皮细胞的更新进行了观察。实验表明,正常情况下,大鼠鼻咽底壁鳞状上皮细胞移动的变化是“S”形曲线,其方程式为 y=ap_0/bp_0 (a-bp_0)e~(-a(x/72));二亚处理后,大鼠鼻咽底壁鳞状上皮细胞移动表现为抛物线,其方程式为 y=a bx cx~2。同时对食道、颊部粘膜的细胞移动进行了观察。     

汞、镉、铜及有机氯对甲螨存活影响的初步研究

于实验室观察汞、镉、铜及有机氯杀虫剂对7种甲螨存活的影响,结果表明,上述有害物质对甲螨的存活并非全为负影响,有的还促进其存活而起正影响,正负影响的因素主要取决于甲螨的种类。作者这一结果能为利用甲螨作为监测土壤污染的生物指标提供依据。     

A SURVEY OF THE PARASITES OF DOGS, CATS AND RATS MADE AT CHENGTU, SZECHWAN, CHINA* PART II. PARASITES OF CATS

In this investigation 100 cats were examined. Of this number 72 were males and 18 females and 10 not recorded as to sex. The pre- ponderance of males is because male cats are in great demand and females are not for sale but kept for breeding purposes. Most of the cats examined were adults. Only a few cats were''free of parasites while mostof them harbored more than one species. The number of dif ferent species of parasites found in individual animals is given below in Table 2 and the results of Andrews'' survey in Shanghai is appended for comparison. From this table it is clear that the Chengtu cats tend to harbor more species of parasites than the cats of Shanghai.     

2,6-二卤苯胺合成方法的改进

目的 尝试用过硼酸钠替代过氧化氢合成2,6-二卤苯胺.方法 对氨基苯磺酰胺与卤化氢在过硼酸钠存在下进行反应,制备3,5-二卤-4-氨基苯磺酰胺,再经水解得2,6-二卤苯胺.结果 成功地合成了目标化合物.结论 此改进可以准确控制投料量,提高最终产物的纯度,收率亦佳.     

INCIDENCE, ETIOLOGY AND PROGNOSIS OF ARRHYTHMIAS ANALYSIS OF 2,530 HOSPITALIZED CASES

This is a report of 2,5301 cases of hospitalized pediatric arrhythmia patients. There were 793 ectopic arrhythmias with conductrive dist.urb- ances accounting for 23.Wo of all 3,351 electro- cardiograms made during the same period, indicating that arrhythmia is common in hos- pitalized children. The common types of pediatric arrhythmia are A-V block, premature beats of ventricular and supraventricular origin, incomplete right bundle branch block and paroxysmal supraven- tricular tachycardia The common causes of pediatric arrhythmia are infectious toxic myocarditis, theumatic fever, theumatic heart disease and digitalis intoxicatio.n, indicating a close correlation between infection and the developmeint of pediatric arrhythmia. So management of infectious diseases plays an important role in the prevention and treat- ment of the disease. The prognosis is generally good with a death rate of only about lTo. Arrhythmias disappear following control of the primary disease.     

精液与血液PGM_1亚型相关性和男性不育症与PGM_1亚型的相关性

用等电聚焦电泳技术测定98例精液/血液标本对葡萄糖磷酸变位酶(phosphoglucomutase,简称PGM_1)亚型的结果,证明同一男性个体血液和精液的PGM_1表型完全一致。同时,测定了171例男性不育症患者的PGM_1亚型。其结果与广州地区正常人群组的比较后,可见男性不育症组的PGM_11~-,1~-2~-,2~-三型的频率较正常人群高,具有显著性意义。在精子数低于0.4亿/ml的患者中,这3型的频率更高。14例无精子精液同样可以测出其PGM_1亚型,说明精液中的PGM_1酶活性,除精子外,还有其他来源。     

OVEREXPRESSION OF p53, EGFR,C-ERBB2 AND C-ERBB3 IN ENDOMETRIOID CARCINOMA OF THE OVARY

Objectives. To determine overexpression of p53, EGFR, e-erbD-2 and e-erbB-3 in endometrioid carcinoma of the ovary and to evaluate the prognostic signiFtcance of these resdts, especially, eoexlsting overexpression of p53 and one of the member of type I growth factor receptor family. Methods. Overexpressions of the p53, EGFR, e-erbB-2 and e-erbB-3 protein were studied by immunohlstochemistry in paraffin-embedded tumor tissue from 28 patients with endometrioid carcinoma of the ovary. Results. 11 (39.3%), 13 (46.4%), and 14 (50. 0%) were stained positively with p53, e-erbB-2 and c-erbB-3 monoclonal ant/bodies. 13 (46. 4%) was stained positively with EGFR polyclonal antibody.There were no relationship between p53, EGFR, C-erbB-2, c-erbB-3 and histologic grade, lymph node metastasis. The percentage of tumors with over expression of p53, EGFR, C-erbB-2 and c-erbB-3 was higher in those with stage Ⅰ～Ⅱ tumors compared with those with stage Ⅰ, in patients with residual tumor after initial surgery compared with those without. A high survival rate was observed in patients without p53, EGFR, c-erbB-2 and c-erbB-3 overexpression respectively than those with. A highest survival rate was observed in patients with both p53 and one of EGFR, c-erbB-2 and c-erbB-3 negative compared with those both positive or either of both positive. Concltrsion. Overexpression of p53, EGFR, c-erbB-2 and C-erbB-3 resulted in a poorer prognosis respectively. Overexpression of both p53 and one of the EGFR, c-erbB-2 and e-erbB-3 is a worse prognostic indicator in patients with endometrioid carcinoma of the ovary.     

LONG-TERM EFFECTS OF HOME-MADE, YJF,VODOPA IN 43 CASES OF PARALYSIS AGITANS

Levodopa (L-dopa) is eifcctive in 30 (69.8%} of 43 cases of paralysis agitans (PA) or Park- inson's disease, giving excelknt results in 8, good results in 15 and fair results in 7. In 12 of the 30 medieatiou effect tapered during 2 year long term therapy. Lohg-term L-dopa medication does not arrest progression of the disease; Long-term mcdieation side effects include dyskincsia which is tolerable in most patiwts, "on-off" alternating dyskinesia and akinesia, kinesia paradoxica and mental symptoms in our series. L-dopa is required in large tlosagcs. it is expensive. most of the dose ingested does not; reach fi\e brain where It is needed but enters extracerebral tissue where doparrnne and some of its metabolites can induw many side effects as gasfrointestinal and cardiovascular reactions although the latter arc not serious enough to cause ECG changes in our series. Cumpouiids of dopa dccarhoxylase inhibitor and L-dopa available in 2 preparations give better results than L-tlopa alone Imt do not re- duce intracerebral sIde eiTects as dyskinesia and mental syruptoms. Dopaminergic receptor agonists also give encouragiug result,s. We plari to do further research on these drugs.     

CLINICAL EVALUATION OF PRAZIQUANTEL IN TREATMENT OF SCHISTOSOMIASIS JAPONICA A RF,PORT OF 181 CASFJS

Praziquantel is an isoquinolin-pyrazine de- rivative of a new heterocyclic system. This paper deals with the clinical observations on 181 cases of schistosomiasis japonica treated with praziquanteL The cure rate was as high as 99.5To. The main side effects included dizziness, headache, lassitude and pain of limbs. The results of various related tests and ECG findings are des- crioed in some detaiL The drug can well be tolerated by the patient without any toxic reac- tion. Therefore, it meets all the requirements of a new potent antischistosomal drug for mass treatment.