Inhibition of airway inflammation by the roots of Angelica decursiva and its constituent,columbianadin

Ethnopharmacological relevance

The roots of Angelica decursiva Fr. Et Sav (Umbelliferae) have been frequently used in traditional medicine as anti-inflammatory, antitussive, analgesic agents and expectorant, especially for treating cough, asthma, bronchitis and upper respiratory tract infections. To establish the scientific rationale for the clinical use of Angelica decursiva and to identify new agents for treating inflammatory lung disorders, pharmacological evaluation of the roots of Angelica decursiva and the isolated constituents was performed.

Methods

In vitro study was carried out using two lung cells, lung epithelial cells (A549) and alveolar macrophages (MH-S). The inflammatory markers such as IL-6 and nitric oxide (NO) for each cell line were examined. For in vivo study, a mouse model of lipopolysaccharide (LPS)-induced acute lung injury was used and the effects on lung inflammation were established by measuring the cell numbers in bronchoalveolar lavage fluid (BALF) and by histological observation.

Results

Water and 70% ethanol extracts of the roots of Angelica decursiva showed considerable inhibitory activity against LPS-induced lung inflammation in mice following oral administration at a dose of 400 mg/kg. Five coumarin derivatives including columbianadin, umbelliferone, umbelliferone 6-carboxylic acid, nodakenin and nodakenetin were isolated. Among the isolated compounds, columbianadin was found to possess strong inhibitory activity against the inflammatory response of IL-1β-treated A549 cells and LPS-treated MH-S cells. Columbianadin was found to inhibit NO production by down-regulation of inducible NO synthase. Moreover, columbianadin was also proved to possess significant inhibitory activity against LPS-induced lung inflammation following oral administration at a dose of 20–60 mg/kg.

Conclusions

The roots of Angelica decursiva were proved to be effective in the treatment of lung inflammation. Columbianadin can be a potential new agent for treating inflammatory lung disorders.     

Extract isolated from Angelica hirsutiflora with insulin secretagogue activity

Ethnopharmacological relevance

Angelica genus (Umbelliferae) has traditionally been used as the medicine and health food considered alleviating several disorders including diabetes mellitus. Angelica hirsutiflora Liu Chao & Chuang is an endemic species and a folk medicine in Taiwan.

Aim of the study

The scientific evidence of anti-diabetic effect for Angelica hirsutiflora remains unknown. The methanolic extracts isolated from Angelica hirsutiflora were studied for its insulin secretagogue and hypoglycemic activities.

Materials and methods

The in vitro effects and possible mechanisms of Angelica hirsutiflora extract on the insulin secretion in isolated mouse and human islets and pancreatic β-cell line HIT-T15 were determined; and tested the regulation of blood glucose in fasted mice and high-fat diet-induced diabetic mice.

Results

Angelica hirsutiflora extract potently stimulated the release of insulin from cultured HIT-T15 cells and isolated mouse and human islets. The intracellular calcium levels were also increased in HIT-T15 cells and isolated human islets treated with Angelica hirsutiflora extract. Angelica hirsutiflora extract was capable of enhancing the phosphorylation of extracellular signal-regulated protein kinases (ERK)1/2 protein in HIT-T15 cells. Specific ERK inhibitor PD98059 inhibited the increase of insulin secretion by Angelica hirsutiflora extract in HIT-T15 cells and isolated mouse islets. When Angelica hirsutiflora extract was administered to the fasted mice, it decreased the rise in blood glucose level after starch loading. The plasma insulin level was also increased by Angelica hirsutiflora extract treatment. In high-fat diet-induced diabetic mice, Angelica hirsutiflora extract markedly improved the oral glucose intolerance as compared with the vehicle control.

Conclusions

These findings support that Angelica hirsutiflora extract may be useful in the control of hyperglycemia in non-insulin-dependent diabetes mellitus by acting as an insulin secretagogue.     

紫花前胡中化学成分的研究

采用HP20大孔吸附树脂、ODS、硅胶柱色谱、Sephadex LH-20柱色谱和半制备HPLC等色谱分离手段对紫花前胡的化学成分进行研究。结合理化性质及MS,NMR等谱学数据鉴定化合物的结构,从紫花前胡80%乙醇提取物中分离并鉴定了12个化合物,分别是(9R,10R)-9-acetoxy-8,8-dimethyl-9,10-dihydro-2H,8H-benzo[1,2-b:3,4-b']dipyran-2-one-10-yl ester(1)、补骨脂呋喃香豆精(2)、顺式-3',4'-二千里光酰基-3',4'-二氢邪蒿内酯(3)、(3'R,4'R)-3'-angeloyloxy-4'-senecioyloxy-3',4'-dihydroseselicalipteryxin(4)、(+)-8,9-dihydro-8-(2-hydro-xypropan-2-yl)-2-oxo-2H-furo[2,3h]chromen-9-yl-3-methylbut-2-enoate(5)、libanoridin(6)、丝立尼亭(7)、花椒素(8)、crocatone(9)、peujaponisinol B(10)、peujaponisinol A(11)、ostenol(12)。其中,化合物1~5为首次从当归属植物中分离得到,化合物7~12为首次从紫花前胡中分离得到。     

The production of nitric oxide and prostaglandin E2 in peritoneal macrophages is inhibited by Andrographis paniculata, Angelica sinensis and Morus alba ethyl acetate fractions

Aim of the study

Traditional Chinese medicine herbs (TCMHs) are used in medicines as well as in daily dietary supplements in Asia. In this study, we employed pNF-κB-Luc or pIFN-γ-Luc and BALB/c mice peritoneal macrophages or splenocytes to investigate both the immune and inflammatory effects of six selected plant species.

Materials and Methods

Specifically, we used ethyl acetate fractions of Astragalus membranaceus (Fisch.) Bunge var. mongholicus (Bunge) Hsiao (Fabaceae) (AM), Andrographis paniculata (Burm. f.) Nees (Acanthaceae) (AP), Angelica sinensis (Oliv.) Diels (Apiaceae) (AS), Eucommia ulmodes Oliv. (Eucommiaceae) leaves (EU leaves), Isatis indigotica Fort. (Brassicaceae) (II) and Morus alba L. (Moraceae) (MA).

Results

We found that ethyl acetate fractions of AP, AS and MA significantly decreased NF-κB luciferase activity and also the secretion of NO and PGE₂ in LPS/IFN-γ stimulated mouse peritoneal macrophages (p < 0.05). In contrast, they did not affect IFN-γ luciferase activity or IFN-γ production in concanavalin A (Con A)-activated mouse splenocytes. Our results indicated that the anti-inflammatory properties of these plant extracts might be resulted from the inhibition of pro-inflammatory mediators (e.g., NO and PGE₂), at least in part via suppression of a signaling pathway such as NF-κB.

Conclusions

Collectively, we have found that three potent bioactive TCMH species exerted significant NF-κB inhibitory activity and acted in a cell type dependent fashion.     

紫金砂药材的指纹图谱研究

目的:建立紫金砂药材的高效液相指纹图谱方法。方法:采用Kromasil 100A C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水为流动相梯度洗脱,流速为1 mL.min-1,检测波长为254 nm。以异欧前胡素为参照物,对10批紫金砂药材进行指纹图谱分析。结果:建立了紫金砂的HPLC指纹图谱共有模式,标定了8个共有峰,10批药材的相似度均>0.9。结论:该方法简便、准确、重复性好,为紫金砂药材的品质评价和质量控制标准的制定提供科学依据。     

The anti-ulcer activities of bisabolangelone from Angelica polymorpha

Aim of the study

Evaluate the anti-ulcer effects of bisabolangelone from Angelica polymorpha Maxim and provide the basic data to further study for the Angelica polymorpha and bisabolangelone.

Materials and methods

Bisabolangelone was isolated from Angelica polymorpha Maxim collected from Shennongjia Forest District of China. The structure of bisabolangelone was elucidated by NMR and MS spectrums. The anti-ulcer effects were evaluated with length of lesion (mm) and activity of H⁺/K⁺-ATPase in two models induced by ethanol and Pylorus ligation. Experimental groups were administered with different doses of bisabolangelone (3.8, 7.6 and 15.3 mg/kg). The positive control group was administered omeprazole with a dose of 3.3 mg/kg.

Results

Bisabolangelone significantly reduced the length of lesion (3.8, 7.6 and 15.3 mg/kg, P < 0.01), inhibited the activity of H⁺/K⁺-ATPase (3.8, 7.6 and 15.3 mg/kg, P < 0.01), decreased the volume of gastric juice (7.6 and 15.3 mg/kg, P < 0.05), and increased the pH value of gastric juice (7.6 and 15.3 mg/kg, P < 0.01, 3.8 mg/kg, P < 0.05).

Conclusions

Bisabolangelone is the main anti-ulcer active compound of Angelica polymorpha, and remarkably preventive and therapeutic action on gastric ulcer. It is possible that bisabolangelone inhibited the activity of the H⁺/K⁺-ATPase, then reducing the secretion of H⁺, and the anti-ulcer mechanism of bisabolangelone was deserved to be further studied.     

Synergistic vasorelaxant and antihypertensive effects of Ligusticum wallichii and Angelica gigas

Aim of the study

The synergistic vasorelaxant and antihypertensive effects of Ligusticum wallichii and Angelica gigas were examined in isolated rat aorta rings and spontaneously hypertensive rats (SHRs).

Materials and methods

The ethanol extract of Ligusticum wallichii (LwEx) or Angelica gigas (AgEx) or their combinations at ratios Ligusticum wallichii:Angelica gigas = 1:1 (MxEx11), 1:3 (MxEx13), and 3:1 (and MxEx31), and their successive water soluble (LwDw, AgDw, MxDw11, MxDw13 and MxDw31) or n-butanol soluble fractions (LwBt, AgBt, MxBt11, MxBt13, and MxBt31) were examined for their vasorelaxant effects. In an antihypertensive study, LwEx, AgEx, or MxEx11 (100 mg/kg) was orally administered to SHRs, and the systolic, diastolic, and mean blood pressure were measured using the tail-cuff method before and 1, 3, 5, 7, and 24 h after oral administration.

Results

Each of the ethanol extracts caused long-term relaxation in endothelium-intact or endothelium-denuded rat aorta preconstricted with norepinephrine (NE, 300 nM). All of the water phases of the ethanol extracts elicited an endothelium-dependent acute relaxation, and the water phase of MxDw11 (EC₅₀ values: 1.08 mg/mL, P < 0.05) had the highest activity. MxDw11-induced acute relaxation was abolished by pretreatment with N^G-nitro-l-arginine (10 μM), methylene blue (1.0 μM), or atropine (0.1 μM), indicating that the response to MxDw involves the enhancement of the nitric oxide-cGMP system. On the other hand, all of the butanol phases showed an endothelium-independent long-term relaxation, and MxBt11 (85 ± 7% relaxation of NE-preconstricted active tone at 20 min after the addition, P < 0.05) displayed the highest activity. MxBt11-induced gradual relaxation was significantly attenuated by an inward rectifier potassium-channel inhibitor, but not by an ATP-sensitive or a large conductance Ca²⁺-activated potassium-channel blocker. Calcium concentration-dependent contraction curves in high-potassium, depolarizing medium were shifted significantly to the right and downward after incubation with MxBt11 (0.03, 0.1, and 0.3 mg/mL), implying that MxBt11 is also involved in the inhibition of extracellular calcium influx to vascular smooth muscle. MxEx11 (100 mg/kg) significantly reduced systolic blood pressure of SHRs at 3, 5, and 7 h after oral administration, but this effect was not induced by Ligusticum wallichii or Angelica gigas alone.

Conclusions

The combination of Ligusticum wallichii and Angelica gigas elicits a synergistic effect on vasorelaxation in isolated rat aortas and antihypertension in SHRs. The ratio of Ligusticum wallichii:Angelica gigas = 1:1 was the most effective of all combinations tested.     

明日叶的化学成分

目的: 对明日叶中的化学成分进行研究。方法: 利用硅胶柱色谱和葡聚糖凝胶Sephadex LH-20柱色谱进行分离纯化;利用IR,NMR和MS等波谱方法对各化合物进行结构鉴定。结果: 从明日叶中分离得到7个化合物,分别鉴定为正二十六烷醇(1),胡萝卜苷(2),豆甾醇(3),槲皮素-3-O-β-D-葡萄糖苷(4),木犀草素-7-鼠李糖葡萄糖苷(5),木犀草素-7-O-α-D-葡萄糖苷(6)和steviol-l3-O-β-glucopyranoside 19-β-glucopyranosyl ester octaacetate(7)。结论: 以上的7个化合物在明日叶中属首次发现。     

当归饮片趁鲜切制的可行性探讨

目的:探讨当归饮片趁鲜切制代替传统切制方法的可行性。方法:采用化学手段,建立多指标定量、定性评价的方法,评价不同方法切制的当归饮片质量。结果:趁鲜切制的饮片其主要药效成分藁本内酯的含量均低于传统的二次软化切制方法制备的饮片,2种方法切制饮片的指纹图谱相似度基本在90%以上。结论:以趁鲜切制法代替传统切制方法似乎有些不妥,2种方法切制的饮片临床效果是否有差别,仍需进一步研究。     

川白芷与祁白芷花粉活力及柱头可授性测定

目的:研究川白芷与祁白芷花粉活力、寿命及柱头可授性,为良种选育提供依据.方法:采用TTC法测定花粉活力及寿命,用联苯胺-过氧化氢法测定柱头可授性.结果:川白芷与祁白芷花蕾期花粉已具有较高活力,随着雄蕊发育,二者活力逐渐增强,花药开裂盛期花粉活力均达85％以上,且花粉活力均随散粉时间延长而逐渐降低.川白芷不同分枝花序的花粉活力差异较小,均维持在85％左右;祁白芷的花粉活力表现为主茎花序＜一级分枝花序＜二级分枝花序,整体来看,空间位置对花粉活力的影响较小.川白芷与祁白芷花粉在室温储藏条件下,开花后第5天花粉活力仍保持约50％,其中川白芷下降幅度大于祁白芷;-20℃条件下可适当延长花粉活力.柱头于开花后第4天逐渐具有可授性,第6天最强,随后逐渐萎蔫变褐,开花后第10天不具可授性.结论:在进行人工授粉时,应选花药开裂盛期的花粉对开花第6天的柱头进行授粉.     

祁白芷挥发油成分的GC-MS分析

目的:研究祁白芷Angelica dahurica cv.Qibaizhi干燥根的挥发油化学成分.方法:采用水蒸气蒸馏法提取祁白芷挥发油,利用气相色谱--质谱联用技术分析挥发油成分.结果:检出290个色谱峰,鉴定出111个化合物,占挥发油总量的90.61%,结论:祁白芷根挥发油中主要成分为:3-蒈烯(3-carene,12.70%),β-榄香烯(β-elemene,6.20%),β-萜品烯(β-ter-pinene,3.53%),β-香叶烯(β-myrcene,l.97%),γ-榄香烯(γ-elemene,l.82%),β-水芹烯(β-phellandrene,l.65%),β-马阿里烯(β-maaliene,1.61%)等.此外,还检出香豆素类成分软木花椒素(suberosin,0.16%).     

Identification of active compounds from Caesalpinia sappan L. extracts suppressing IL-6 production in RAW 264.7 cells by PLS

Ethnopharmacological relevance

Caesalpinia sappan L. is distributed in Southeast Asia and also used as herbal medicine for the treatment of various diseases such as burning sensations, leprosy, dysentery, osteoarthritis and rheumatoid arthritis (RA). The overproduction of IL-6 plays an important role in the prognosis of RA, but the active compounds from the extracts of Caesalpinia sappan L. suppressing IL-6 production remain unknown.

Aims of the study

Identifying the main active compounds of Caesalpinia sappan L. extracts inhibiting the IL-6 production in LPS-stimulated RAW 264.7 cells by partial least squares (PLS).

Materials and methods

Sixty-four samples with different proportions of compounds were prepared from Caesalpinia sappan L. by supercritical CO₂ fluid extraction (SCFE) and refluxing. Each of 64 samples was applied to RAW 264.7 cells with LPS to evaluate whether IL-6 production by LPS is affected by addition of each sample. The IL-6 production in medium was determined by ELISA and the inhibitory activity of each sample was analyzed. In addition, the fingerprints of these 64 samples were also established by ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry (UPLC–MS). We used the PLS, a simplified method, to evaluate the results from IL-6 production and fingerprints.

Results

Each of 64 samples markedly suppressed LPS-induced IL-6 production in RAW cells. The fingerprints by UPLC–MS clearly revealed variations among 64 samples produced in different extract conditions. The PLS analysis with IL-6 production and fingerprints by UPLC–MS suggested that the peaks 71, 93, 150, 157, 168 have more influence on the inhibitory activity of Caesalpinia sappan L. extracts. The peaks 71, 93, 150 are likely representing sappanone A, protosappanin E and neoprotosappanin, respectively. The peaks 157 and 168 are still at large.

Conclusion

This is the first report that sappanone A, protosappanin E, neoprotosappanin and two unidentified compounds can be considered as possible active compounds that might inhibit IL-6 production. Further studies are needed to confirm the effectiveness of these five compounds on IL-6 production and possible mechanism.     

对照提取物用于当归药材质量控制的研究

为提高当归药材的质量标准,解决部分对照品稀缺问题,进行了以对照提取物为对照的当归药材质量控制研究。采用色谱分离技术制备对照提取物,并对其中3个指标成分阿魏酸、洋川芎内酯I、洋川芎内酯H进行标定,建立了以对照提取物为对照的当归药材UPLC含量测定方法,并采用t检验对以对照提取物和对照品为对照的测定结果进行比较,结果基本一致,该方法可用于当归药材质量控制,且经济实用,本研究初步验证了其可行性。     

白芷、石菖蒲对冲和膏处方中主要成分经皮渗透作用的影响

目的:考察佐使药白芷、石菖蒲对冲和膏中主要成分经皮渗透的影响.方法:采用改良Franz透皮扩散池,大鼠离体皮肤为渗透屏障,以芍药苷和蛇床子素为指标,HPLC测定其含量,分别考察白芷、石菖蒲及其配伍联用对冲和膏主要成分促透作用的影响.结果:白芷、石菖蒲单用对芍药苷经皮渗透的增渗倍数分别为0.84,1.02,对蛇床子素的增渗倍数分别为1.54,0.79;配伍联用后芍药苷和蛇床子素的经皮渗透均达到最佳水平,增渗倍数分别1.00,1.89.结论:白芷、石菖蒲配伍联用可促进主要成分的经皮渗透,对冲和膏处方药效的发挥具有重要的佐使作用.     

Identification of rosmarinic acid as the major active constituent in Cordia americana

Ethnopharmacological relevance

Preparation from leaves of Cordia americana have been widely used in traditional medicine in South Brazil to treat wounds and various inflammations.

Aim of the study

The objective of this work was to identify the effective compounds in the ethanolic extract prepared from the leaves of Cordia americana, which is used in traditional South Brazilian medicine as anti-inflammatory and wound healing remedy.

Materials and methods

Isolation and structure elucidation techniques were performed in order to identify the compounds of Cordia americana and HPLC analysis was used for the quantification. The major constituent and the ethanolic extract were investigated for inhibition of 5-lipoxygenase, p38α MAPK, TNFα release and NF-κB as well as in the fibroblast scratch assay.

Results

Rosmarinic acid (1) was identified as the major compound with an amount of 8.44% in the ethanolic extract of the leaves of Cordia americana. The ethanolic extract as well as (1) exhibited the highest inhibitory effects on 5-lipoxygenase (IC₅₀ = 0.69 and 0.97 μg/mL, resp., IC₅₀ of BWA4C as reference: 0.3 μM) and p38α (IC₅₀ = 3.25 and 1.16 μg/mL, resp., IC₅₀ of SB203580 as reference: 0.046 μM) and moderate inhibitory effects on TNFα release. Slight effects were observed in the fibroblast scratch assay.

Conclusions

This study increases our knowledge on the effective compound in Cordia americana and supports its use in traditional medicine. We demonstrated for the first time pharmacological effects of Cordia americana and we provide evidences for a crucial role of rosmarinic acid as the major key player.     

川白芷花药培养技术体系的建立

目的:探讨川白芷花药培养中愈伤组织诱导和植株再生条件.方法:以川白芷花药为外植体,以MS为基本培养基,研究低温(4℃)预处理时间、光暗培养条件以及不同激素配比对川白芷花药培养愈伤组织诱导和植株再生的影响.结果:不经过低温预处理的花药诱导率最高,低温预处理2d的花药诱导率次之;暗培养为最佳培养条件;诱导愈伤组织的最佳培养基为MS+2.0mg·L-12,4-D+1.0mg·L-16-BA,愈伤组织诱导率可达38.89％;MS +0.5 mg·L-1NAA+1.5 mg·L-1KT+10mg· L-1AgNO3适于不定芽的诱导,不定芽转入附加0.5 mg·L-1IBA的1/2 MS的生根培养基上,生根后获得完整的再生植株.结论:初步建立了川白芷花药培养愈伤组织诱导及植株再生体系.     

Topical anti-inflammatory activity of body fat from the lizard Tupinambis merianae

Aim of the study

Tupinambis merianae is one of the most frequently used animals in traditional medicine in Brazil as its body fat has many different therapeutic indications as a folk remedy. The present study evaluated the topical anti-inflammatory activity of Tupinambis merianae fat in treating ear edema in an animal model.

Materials and methods

Oil (OTM) was extracted from body fat located in the ventral region of Tupinambis merianae using hexane as a solvent. Ear edema provoked by the application of croton oil (single and multiple application), arachidonic acid, phenol and capsaicin to Swiss mice was used to evaluate the potential anti-inflammatory value of the topical use of OTM. Topical applications of these irritants were made to the right ears of animals that were pre-treated with a acetone (control), dexamethasone, or OTM.

Results

Our results indicate that Tupinambis merianae fat demonstrates significant topical anti-inflammatory activity that reduces inflammation related to ear edema caused by croton oil (single and multiple application), arachidonic acid, phenol. While the inflammation caused by capsaicin, OTM did not significantly reduce the edema.

Conclusion

This represents the first evidence of anti-inflammatory activity of Tupinambis merianae fat in in vivo models, confirming its usefulness in traditional medicine as a remedy for skin diseases (dermatitis). The fatty acids present in OTM most likely inhibit the response pathway of arachidonic acid and its metabolites, thus reducing the production of pro-inflammatory mediators.     

祁白芷根中新的天然产物

目的:研究祁白芷根脂溶性化学成分。方法:应用溶剂法和色谱法分离纯化化合物,利用谱学技术鉴定化合物结构。结果:分离得到27个化合物,分别鉴定为异欧前胡素(1)、别异欧前胡素(2)、水合氧化前胡素(3)、比克白芷素(4)、别欧前胡素(5)、β-谷甾醇(6)、邻苯二甲酸二丁酯(7)、7-羟基-6-(3-甲基-2-丁烯氧基)-香豆素(8)、花椒毒酚(9)、5-羟基-8-甲氧基补骨脂素(10)、奥氏芹二醇(11)、伞形花内酯(12)、香柑内酯(13)、欧前胡素(14)、珊瑚菜内酯(15)、异茴芹素(16)、6-乙酰基-7-甲氧基-香豆素(17)、补骨脂素(18)、异东莨菪内酯(19)、滨蒿内酯(20)、花椒毒素(21)、当归醇A(22)、氧化前胡素乙醇醚(23)、去氢柳叶白姜花内酯(24)、尤劳帕替醇(25)、独活属醇(26)、栓翅芹烯醇(27)。结论:化合物8为新的天然产物;除化合物1,3,14外,其余化合物均为首次从祁白芷根中分离得到。     

当归多糖抑制氧化损伤延缓造血干细胞衰老

目的:观察当归多糖(ASP)对小鼠造血干细胞(HSCs)活性氧(ROS)、总抗氧化能力(T-AOC)及p16 mRNA表达的影响,探讨ASP延缓HSCs衰老的可能机制.方法:C57BL/6J小鼠随机分为正常组、衰老组,并分别予ASP干预;衰老组采用X线3.0 Gy/8 F全身均匀照射,建立小鼠HSCs衰老模型;正常、衰老ASP干预组在建模期间均予ASP灌胃;而正常组、衰老组予等体积NS灌胃.免疫磁珠分离HSCs.通过β-半乳糖苷酶(SA-β-Gal)染色、细胞周期分析和混合集落培养观察HSCs生物学特性变化;流式细胞术与免疫荧光检测ROS产量;比色法检测T-AOC;荧光定量PCR检测p16 mRNA表达.结果:与正常组比较,3.0 Gy/8 F的X线能显著增加衰老组HSCs SA-β-Gal染色阳性率和G1期比例;增加ROS产量,上调p16 mRNA表达;混合集落形成能力和T-AOC下降.与衰老组比较,ASP能显著降低衰老组HSCs的SA-β-Gal染色阳性率和G1期比例,减少ROS产量,下调p16 mRNA;增加混合集落形成能力和T-AOC.结论:3.0 Cy/8 F的X线能诱导小鼠HSCs衰老;ASP则能通过抑制氧化损伤及下调p16 mRNA表达延缓小鼠HSCs衰老.