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1.
Mehmet Karaca Fehmi Odabasoglu Yakup Kumtepe Abdulmecit Albayrak Elif Cadirci Osman N. Keles 《European journal of obstetrics, gynecology, and reproductive biology》2009
Objectives
To evaluate the effects of erythropoietin (EPO) as an antioxidant and tissue protective agent and study the biochemical and histopathological changes in experimental ischemia and ischemia/reperfusion (I/R) injury in rat ovaries.Study design
36 Adult female rats were used. The experimental groups were designed as Group 1: sham operation; Group 2: bilateral ovarian ischemia; and Group 3: 3 h period of ischemia followed by 3 h reperfusion. Group 4 rats were administered a 5000 IU dose of EPO, before 0.5 h of ischemia, and then bilateral ovarian ischemia was applied. After a 3 h period of ischemia, the bilateral ovaries were removed. In Group 5, a 3 h period of bilateral ovarian ischemia was applied. 2.5 h after the induction of ischemia, the rats were administered the same dose of EPO. At the end of a 3 h period of ischemia, 3 h reperfusion was continued after the ovaries were removed. Group 6 underwent a sham operation after administration of 5000 IU/kg of EPO. After the experiments, superoxide dismutase (SOD), inducible nitric oxide synthase (iNOS), and myeloperoxidase (MPO) activity were determined, and histopathological changes were examined in all rat ovarian tissue.Results
Ischemia and ischemia/reperfusion increased the iNOS and MPO activity while decreasing the SOD activity significantly in comparison to the sham group. The 5000 IU/kg of EPO before ischemia and I/R reversed the trend in iNOS and MPO activities. The levels of SOD were decreased by the ischemia and I/R. The administration of EPO before ischemia and I/R treatments also reversed the trend in the SOD levels. In the ischemia/reperfusion plus EPO groups, though we observed minimal vascular dilation in the ovary stroma and some degenerative cell clusters, most of cellular structures did not show any pathological changes.Conclusions
Administration of EPO is effective in reversing tissue damage induced by ischemia and/or ischemia/reperfusion in ovaries. 相似文献2.
Yigiter M Halici Z Odabasoglu F Keles ON Atalay F Unal B Salman AB 《European journal of obstetrics, gynecology, and reproductive biology》2011,157(1):94-100
Objective
To evaluate the effects of growth hormone (GH) as an antioxidant and tissue-protective agent and analyse the biochemical and histopathological changes in rat ovaries due to experimental ischemia and ischemia/reperfusion injury.Study design
Forty-eight adult female rats were randomly divided into eight groups. In Group 1, a period of bilateral ovarian ischemia was applied. In Groups 2 and 3, 1 and 2 mg/kg of GH was administered, and 30 min later, bilateral ovarian ischemia was applied (after a 3-h period of ischemia, both ovaries were surgically removed). Group 4 received a 3-h period of ischemia followed by 3 h of reperfusion. Groups 5 and 6 received 1 and 2 mg/kg of GH, respectively, 2.5 h after the induction of ischemia. At the end of a 3-h period of ischemia, bilateral vascular clips were removed, and 3 h of reperfusion continued. Group 7 received a sham operation plus 2 mg/kg of GH. Group 8 received a sham operation only. After the experiments, superoxide dismutase and myeloperoxidase activity and levels of glutathione and lipid peroxidation were determined, and histopathological changes were examined in all rat ovarian tissue.Results
Ischemia and ischemia/reperfusion decreased superoxide dismutase activity and glutathione levels in ovarian tissue, but increased lipid peroxidation levels and myeloperoxidase activity significantly in comparison to the sham group. The 1 and 2 mg/kg doses of GH before ischemia and ischemia/reperfusion decreased lipid peroxidation levels and myeloperoxidase activity in the experimental groups. The administration of GH before ischemia and ischemia/reperfusion treatments also increased superoxide dismutase and glutathione levels. The histopathological findings also suggested a protective role of GH in ischemia/reperfusion injury. That is, ovarian tissues in the ischemia groups showed histopathological changes, such as haemorrhage, cell degeneration, and necrotic and apoptotic cells, but these changes in the GH groups were lesser. Moreover, in the ischemia/reperfusion groups, acute inflammatory processes - such as neutrophil adhesion and migration, apoptotic and degenerative cells, stromal oedema and haemorrhage - were present. However, the ovarian tissues of the IR + GH (1 mg) group had minimal apoptotic cells, and the IR + GH (2 mg) group had no apoptotic cells. In addition, the general ovarian histological structures of these groups were similar to those of the healthy control group.Conclusions
The administration of GH is protective against ischemia and/or ischemia/reperfusion-induced ovarian damage. This protective effect can be attributed to the antioxidant properties of GH. 相似文献3.
Turhan Aran Suleyman Guven Mesut A. Unsal Ahmet Alver Ahmet Mentese Esin Yulug 《European journal of obstetrics, gynecology, and reproductive biology》2010
Objective
To evaluate the effect of ovarian torsion on serum levels of ischemia-modified albumin (IMA) in an experimental model.Study design
Sixteen female adult Sprague–Dawley rats were involved in the study. Rats were allocated randomly to group I or group II on the day of the experiment. Group I (eight rats) comprised the control (sham operated) group. In group II (eight rats), a torsion model was created by using atraumatic vascular clips just above and below the right ovary. At the end of a 3-h period of ischemia, the ovaries were removed. Blood was sampled before and after operation to assess serum IMA levels. Serum IMA levels (absorbance units) and histopathologic damage scores were evaluated.Results
Initial serum IMA levels were similar in both groups. After the operation, significant elevation was observed in group II in contrast to group I (0.191 ± 0.034 and 0.277 ± 0.089 ABSU, p = 0.05). Histologic specimens of the ovaries in group II had higher scores of follicular cell degeneration, vascular congestion, hemorrhage and inflammatory cell infiltration than those in group I (p < 0.001).Conclusions
The elevated serum IMA levels observed in the ovarian torsion model seem to have a potential role as a serum marker in the early diagnosis of ovarian torsion. 相似文献4.
Remzi Abali Nicel Tasdemir Mehmet Aytac Yuksel Savas Guzel Meltem Oznur Burcin Nalbantoglu Ufuk Goker Tasdemir 《European journal of obstetrics, gynecology, and reproductive biology》2013
Objective
The aim of this study was to investigate the effect of infliximab on experimentally induced ovarian ischemia/reperfusion injury (IRi).Study design
A total of 42 female rats were equally divided into 6 experimental groups; group 1: sham operation, group 2: 3-h ischemia, group 3 and 4: 3-h ischemia, 3-h reperfusion, group 5 and 6: 3-h ischemia, 24 h reperfusion. In group 4 and group 6, 30 min before reperfusion, infliximab was administered intraperitoneally at a dose of 5 mg/kg. Bilateral ovaries were removed for histopathologic and biochemical analysis. Serum MDA (sMDA), tissue MDA (tMDA), serum NO (sNO), tissue NO (tNO) and serum catalase concentrations were analyzed. Tissue damage of ovarian tissue was scored by histological examination.Results
The infliximab administration significantly lowered the sNO, tNO and sMDA concentrations in group 4 compared to group 3 (p = 0.041, p = 0.025 and p = 0.035, respectively). sNO, tNO and sMDA concentrations were also lower in group 6 when compared to group 5, but this differences were not significant (p > 0.05). On the other hand, tMDA concentrations were lower in infliximab-applied groups when compared to ischemia/reperfusion groups (group 3 vs. 4 and 5 vs. 6) (p = 0.045 and p = 0.048, respectively). Moreover, histopathologic tissue damage scores in infliximab administration groups were significantly lower than in ischemia/reperfusion groups (p < 0.001).Conclusion
Infliximab attenuates I/R-induced ovarian tissue injury in rats subjected to ischemia/reperfusion. 相似文献5.
B. Cekic A. Besir E. Yulug S. Geze M. Alkanat 《European journal of obstetrics, gynecology, and reproductive biology》2013
Objectives
To determine the effects of dexmedetomidine on pneuomoperitoneum-related ischaemia–reperfusion (I/R) injury in rat ovarian tissue.Study design
Animals were randomized into three groups: Group S (n = 8), no pneumoperitoneum; Group C (n = 8), pneumoperitoneum; and Group D (n = 8), 100 μg intraperitoneal dexmedetomidine 30 min before pneumoperitoneum. Ovarian tissue was collected from all rats 30 min after desufflation, and fresh frozen for histological and biochemical evaluation.Results
Body weight was similar in all three groups (202.62 ± 28.86, 211.00 ± 14.45 and 212.87 ± 15.71 g in Groups S, D and C, respectively). The mean malondialdehyde level was higher in Group C than the other groups (p < 0.03). When the histological samples of ovarian tissue were compared, vascular congestion, haemorrhage, follicular cell degeneration and infiltrative cell infiltration scores were higher in Group C compared with the other groups (p < 0.05). Significantly lower scores for the histological parameters were found in Group D compared with Group C (p < 0.05). Similar scores for follicular cell degeneration and inflammatory cell infiltration were found in Group D and Group S (p > 0.05). Although vascular congestion and haemorrhage scores were significantly lower compared with Group C, higher scores were found for Group D compared with Group S (p < 0.05).Conclusion
Pneumoperitoneum caused oxidative injury in rat ovarian tissue. Dexmedetomidine reduced oxidative stress and histological injury related to I/R. 相似文献6.
Andy Petroianu Luiz R. AlbertiLeonardo S. Vasconcellos 《European journal of obstetrics, gynecology, and reproductive biology》2007
Objective
The objective was to assess the natural pregnancy of rabbits subjected to bilateral oophorectomy and orthotopic allogeneic intact and sliced ovarian transplantation without a vascular pedicle, and to determine the morphofunctional aspects of the transplanted ovaries.Study design
Thirty-two female rabbits were studied. The ovaries were removed and orthotopically transplanted without vascular anastomoses between the two breeds of rabbits. In Group 1 (n = 8), only laparotomy and laparorrhaphy were performed, in Group 2A (n = 8) intact ovaries were transplanted on both sides, in Group 2B (n = 8) both ovaries were sliced and orthotopically transplanted, and in Group 2C (n = 8) an intact ovary was transplanted on one side and a sliced ovary on the other side. Three months later, the animals were paired with males for copulation. Estradiol, progesterone, follicle-stimulating hormone, and luteinizing hormone levels were assessed. A histologic study was carried out and the numbers of pregnancies and litters were also determined.Results
Pregnancies occurred in seven rabbits in Group 1, 37.5% of rabbits in Group 2A, 50% in Group 2B, and 62.5% in Group 2C. Hormone levels and histology confirmed the vitality and function of all ovaries.Conclusions
Intact or sliced orthotopic allogeneic ovarian transplantation without a vascular pedicle is viable in rabbits, and preserves their hormonal and fertile functions. 相似文献7.
Aliskiren – a promising strategy for ovarian ischemia/reperfusion injury protection in rats via RAAS
Yasin Bayir Elif Cadirci Beyzagul Polat Nurcan Kilic Baygutalp Abdulmecit Albayrak Emre Karakus 《Gynecological endocrinology》2016,32(8):675-683
The aim of this study was to evaluate the effects of aliskiren, direct renin inhibitor, as an antioxidant and tissue protective agent and evaluate the molecular, biochemical, and histopathological changes in experimental?ischemia?and?ischemia/reperfusion injury in rat ovaries. Forty-eight female rats were randomly divided into eight groups: in Group 1, only sham operation was performed. Group 2 received 100?mg/kg aliskiren and sham operated. In Group 3, 3?h-period of bilateral ovarian?ischemia?was applied. Group 4 received a 3-h period of?ischemia?followed by 3?h of reperfusion. Groups 5 and 6 received 50 and 100?mg/kg, respectively, of aliskiren and bilateral ovarian?ischemia?was applied (after a 3-h period of ischemia, both ovaries were surgically removed). To Groups 7 and 8, 50 and 100?mg/kg of aliskiren were administered, respectively, and the induction of?ischemia was performed. At the end of a 3-h period of?ischemia, bilateral vascular clips were removed, and 3?h of reperfusion continued. After the experiments, IL-1β, IL-6, TNF-α, and iNOS mRNA expressions and SOD, GSH, MDA, renin, and angiotensin-II levels were determined and histopathological changes were examined in rat ovaries. Aliskiren treatment normalized excessive changes in cytokine and oxidative stress markers in both ischemia and ischemia/reperfusion injury. Histopathologically, treatment with aliskiren ameliorated the development of?ischemia?and/or ischemia/reperfusion tissue injury. This study concluded that aliskiren treatment is effective in reversing ischemia and/or ischemia/reperfusion induced ovary damage via the improvement of oxidative stress, reduction of inflammation, and suppression of the renin-angiotensin aldosterone system. 相似文献
8.
Kara M Daglioglu YK Kuyucu Y Tuli A Tap O 《European journal of obstetrics, gynecology, and reproductive biology》2012,162(2):197-202
Objective
The aim of this study was to investigate the effect of edaravone on experimentally induced ovarian torsion/detorsion ischemia/reperfusion (I/R) injury.Study design
: Forty-six female adult Wistar-Albino rats were utilized to create five groups: In group 1, only 5 mg/kg edaravone was given and ovary torsion was not performed. In group 2, torsion was not performed and no drug was given. In group 3, vehicle was given and torsion/detorsion was performed. In group 4, 1 mg/kg edaravone was given and torsion/detorsion was performed. In group 5, edaravone; 5 mg/kg drug was administered and torsion/detorsion was performed. Right ovarian torsion was simulated for a 3-h period of ischemia and a 1-h reperfusion period. Right ovaries were then surgically extirpated in all groups. In ovarian tissue samples malondialdehyde (MDA) levels and activity of superoxide dismutase were studied. Microscopic ovarian tissue damage was scored by histologic and electron microscopic findings.Results
The MDA level in the group 5 was significantly lower than group 3 (p < 0.001). Superoxide dismutase activity in the group 5 was significantly higher than group 3 (p < 0.001). Histopathological ovarian tissue damage in the group 5 were significantly lower than group 3 (p < 0.001).Conclusion
These results indicate that edaravone could be an effective agent in the short-term treatment and prevention of ovarian ischemia and reperfusion damage. 相似文献9.
Mohamad Sayed Abdellah Ali Mahmoud Mohamad Mostafa Elsaman 《European journal of obstetrics, gynecology, and reproductive biology》2012
Objective
The aim of the trial was to assess the safety and efficacy of tricholoroacetic acid for the treatment of dysfunctional uterine bleeding using topical versus intrauterine instillation.Study design
In a pilot prospective randomized clinical trial, seventy women were randomly allocated to one of two groups. In Group I, the patients were subjected to intrauterine instillation of 95% tricholoroacetic acid. Group II underwent dilatation and curettage before topical application of 95% tricholoroacetic acid.Results
The groups were similar regarding baseline clinical characteristics. There was a satisfactory clinical reduction of menstrual flow (amenorrhea, hypomenorrhea and eumenorrhea) at a rate of 97.1% (Group I) and 85.7% (Group II) at 6 months. A significant increase was observed in the mean haemoglobin value at 3 and 6 months in both treatment groups (P < 0.05).Group I showed a significant increase in haemoglobin level compared to Group II (P < 0.05) and a significant reduction of the endometrial thickness compared to Group II (2.21 ± 0.41 versus 3.03 ± 3.37).Conclusion
Trichloroacetic acid use for treating dysfunctional uterine bleeding seems to be efficient and safe, especially in women who do not require conception. Trichloroacetic acid intrauterine instillation is associated with longer duration of reduced menstrual bleeding than topical application. 相似文献10.
Fatih Sendag Ayşin Akdogan Kemal Ozbilgin Gülşen Giray Kemal Oztekin 《European journal of obstetrics, gynecology, and reproductive biology》2010
Objective
To investigate the effect of exogenous ovarian stimulation with human menopausal gonadotropin (hMG) and recombinant follicle stimulating hormone (rFSH) on the expression of integrins alpha(3), beta(1) in the rat endometrium during implantation.Study design
Following three successive normal estrous cycles the animals were divided into five groups: Group I (n = 10, control group) received no medication; Group II (n = 10) received 10 units of hMG; Group III (n = 10) received 20 units of hMG; Group IV (n = 10) received 10 units of rFSH; Group V (n = 10) received 20 units of rFSH at midday of middiestrous. The rats were then mated with fertile males. The animals were sacrificed on the day of implantation. The uterine horns were placed in fixative and paraffin blocks of the tissue were cut in 5 μm sections. The tissues were stained with primary antibodies; monoclonal anti-integrin alpha(3) and monoclonal anti-integrin beta(1) using immunohistochemical methods. The staining intensities of alpha(3) and beta(1) integrins were calculated separately for epithelium and stroma in each group.Results
Staining intensities of alpha(3) and beta(1) integrins in both the epithelium and the stroma were significantly lower in the treatment groups than the control group (p < 0.05).Conclusion
Ovarian stimulation by low and high doses of HMG and rFSH may have an effect on endometrial receptivity, possibly via a decrease in expression of integrins in the endometrium during the implantation period. 相似文献11.
Salah M. Rasheed Magdy M. Amin Ahmed H. Abd Ellah Ahmed M. Abo Elhassan Mazen A. El Zahry Hala A. Wahab 《International journal of gynaecology and obstetrics》2014
Objective
To evaluate the impact of bilateral internal iliac artery ligation (BIL), bilateral uterine artery ligation (BUAL), step-wise uterine devascularization (SWUD), and B-Lynch on infertility, ovarian reserve, and pregnancy outcome.Methods
The study included 168 infertile or pregnant patients—recruited at outpatient clinics in Egypt—who had previously undergone uterine-sparing surgery (BIL [group I], n = 59; SWUD [group II], n = 65); BUAL [group III], n = 2; and B-Lynch [group IV], n = 42). One-way analysis of variance was used to compare the prevalence of infertility, the status of ovarian reserve, and the prevalence and type of relevant maternal and/or fetal obstetric complications between the groups.Results
Groups II and IV had the highest prevalences of infertility. The ovarian reserve was significantly lower in group II. Unexplained infertility was the predominant cause of infertility in group I, anovulation and premature ovarian failure in group II, and endometriosis and intrauterine adhesions in group IV. The frequency of obstetric complications, particularly placenta previa and preterm labor, was high in group IV.Conclusion
Of the 4 procedures, BIL had the least deleterious effect on reproductive performance; SWUD increased the risk of premature ovarian failure, and B-Lynch increased the risks of endometriosis, intrauterine adhesions, placenta previa, and preterm labor. 相似文献12.
Carla Cristina Maganhin Luiz Fernando Portugal Fuchs Ricardo Santos Simões Ricardo Martins Oliveira-Filho Manuel de Jesus Simões Edmund Chada Baracat José Maria Soares Jr 《European journal of obstetrics, gynecology, and reproductive biology》2013
Objective
To evaluate the histomorphometry and expression of Ki-67 and c-kit in ovarian follicles of pinealectomized or melatonin-treated pinealectomized rats.Study design
Forty adult rats were randomly divided into four groups of 10 animals: Group I – control; Group II – sham-pinealectomized; Group III – pinealectomized (Px), and Group IV – Px treated with melatonin (10 μg/night, per animal). After two months’ treatment, on the night of proestrous, the animals were placed in metabolic cages for night urine collection and subsequent measurement of 6-sulfatoxymelatonin (6-SMT). The rats were anesthetized, blood samples were taken for estrogen and progesterone determinations, and they were then euthanized. The ovaries were dissected out for further histological and immunohistochemical analyses. Data were first submitted to analysis of variance (ANOVA) complemented with the Tukey–Kramer test for multiple comparisons (P < 0.05).Results
The urinary levels of 6-SMT and serum progesterone were lower in the Px group (GIII). Exogenous melatonin treatment restored both blood melatonin and 6-SMT urinary levels. The histomorphometric data in Group III revealed a significant increase of degenerating antral and non-antral follicles with regard to the other groups. In addition no corpora lutea were observed in this group. No significant differences were noticed regarding the number of corpora lutea among the other groups (I, II and IV), but the number of cells and the thickness of the theca interna of Px animals (Group III) were higher than in the other groups. Conversely, the density of progesterone receptors (fmol/g) in the ovaries of Group III was significantly lower than in the other groups.Conclusion
Our data indicate that melatonin exerts a role on the maintenance of a proper follicular function, and is thus important for ovulation and progesterone production. 相似文献13.
Yingfen Ying Lin Wang Yaxia Chen Jingyi Jiang Yinluan Ouyang Hefeng Huang Jian Xu 《European journal of obstetrics, gynecology, and reproductive biology》2013
Objective
To determine whether FTY720 combined with CsA has immunomodulatory effects on human ovarian tissue transplanted to the back muscle of rabbits for an 8-week period.Study design
We selected rabbits as recipients of ovarian xenografts with and without treatment by CsA and FTY720. Ovarian fragments from twelve patients were cut into 2 mm × 2 mm, 1–2 mm thick pieces and randomly distributed into four groups: Group 1 (FTY720 2 mg/kg/d + CsA 3 mg/kg/d), Group 2 (FTY720 1 mg/kg/d + CsA 3 mg/kg/d), Group 3 (FTY720 0.5 mg/kg/d + CsA 3 mg/kg/d) and Group 4 for control (CsA 3 mg/kg/d). FTY720 was started three days before transplantation and was given daily after transplantation. CsA was administrated post-transplantation. All the animals were killed 8 weeks post- transplantation. Levels of serum estrogen (E2), interferon-γ (IFN-γ) and interleukin-4 (IL-4) were detected by radioimmunoassay and ELISA. Anti-CD31 and anti-Ki-67 antibodies were used to evaluate neo-vascularization in xenografts and proliferation activity of ovarian follicles. Peripheral CD4+/CD8+ T cells were analyzed by flow cytometry.Results
Combined treatment with cyclosporin A and FTY720 improved graft survival and reduced peripheral CD4+ and CD8+ T cell counts compared to treatment with cyclosporin A alone. Neovascularization took place in the peripheral zone of the xenograft while granulosa cells, positively stained by Ki-67, were found in early-stage follicles and stromal cells in the combined treatment groups.Conclusion
FTY720 in combination with cyclosporin A maintains human ovarian xenografts in these rabbit models. 相似文献14.
Kara Ozlem Yakan Birkan Kara Mustafa Kaymak Emin 《Taiwanese journal of obstetrics & gynecology》2018,57(6):836-841
Objective
It is aimed to evaluate the protective effect of bilberry on I/R injury in rat ovary.Materials and methods
A total 48 female Wistar–Albino rats were utilized to form five groups: Group 1 (control group) (n = 8), neither drug was given and nor procedure was performed. Group 2 (bilberry control group) (n = 10), single dose 200 mg/kg bilberry was administered by gavage and no procedure was performed. Group 3 (I/R group) (n = 10), no drug was given, 1-h ischemia and 2-h reperfusion was performed. Group 4 (bilberry before I/R group) (n = 10), single dose 200 mg/kg bilberry was administered by gavage before ischemia. Then 1-h ischemia and 2-h reperfusion was performed. Group 5 (bilberry after I/R group) (n = 10), first 1-h ischemia was performed. Single dose 200 mg/kg bilberry was administered by gavage and then 2-h reperfusion was performed. Right ovaries were surgically extirpated in all groups. In ovarian tissue samples, malondialdehyde (MDA) levels and enzymatic activities of superoxide dismutase (SOD), catalase (CAT) were studied. In ovarian tissue samples, DNA damage and apoptosis were assessed by using TUNEL method. Histopathologic examination was performed by light microscopic findings.Results
When group 3 was compared with another groups, MDA levels were significantly higher, enzymatic activities of SOD and CAT were found to be as significantly lower in ovarian tissue and blood (p < 0.001). In histopathologic examination, ovarian tissue damage in the group 3 were significantly higher than other groups (p < 0.001). Also, DNA damage and apoptosis were significantly higher in group 3 than other groups (p < 0.001).Conclusion
Biochemical findings were lower and histopathologic damage was less especially in bilberry before I/R group (group 4). In conclusion, bilberry seems to be effective in prevention of ovarian I/R injury and short-term treatment. 相似文献15.
Jun Kumakiri Iwaho KikuchiRie Ozaki Makoto JinushiAyako Kono Satoru Takeda 《European journal of obstetrics, gynecology, and reproductive biology》2013
Objective
To demonstrate the feasibility of minimally invasive surgery using a novel optical device to treat large benign ovarian cysts and to compare the safety of the procedure with that of conventional laparoscopically assisted multiport extracorporeal cystectomy.Study design
Twenty-one patients with large benign ovarian cysts underwent laparoscopically assisted extracorporeal ovarian cystectomy via a single suprapubic incision using a novel 10-mm rigid laparoscope with an adjustable direction of view and a multiport device, between October 2010 and July 2012. The surgical outcomes were retrospectively compared between these patients (Group A) and 32 patients who underwent the conventional 3-port laparoscopically assisted extracorporeal procedure between January 2009 and September 2010 (Group B). Data were statistically analyzed using the Mann–Whitney U-test or Fisher's exact test.Results
None of Group A required conversion to conventional multiport laparoscopy. The total duration of surgery, elapsed time between skin incision and the start of pneumoperitoneum, and time required for intra- and extra-corporeal manipulations did not significantly differ between the groups. The time required for skin closure, however, was significantly decreased in Group A compared with Group B (13.0 ± 3.5 vs. 20.2 ± 4.8 min, P = 0.005). The volume of blood loss and postoperative blood findings were similar to those associated with the conventional procedure. Postoperative visual analog pain scales at 3 h were significantly lower in Group A than in Group B (3.7 ± 2.6 vs. 4.8 ± 2.0, P = 0.04). Postoperative complications did not arise after either procedure.Conclusion
Laparoscopically assisted extracorporeal cystectomy via a single suprapubic incision is a feasible and safe alternative to conventional multiport cystectomy for treating large benign ovarian cysts. 相似文献16.
Nadav Michaan Gila Ben-DavidDalit Ben-Yosef Beni AlmogAriel Many David PauznerJoseph B. Lessing Ami AmitFoad Azem 《European journal of obstetrics, gynecology, and reproductive biology》2010
Objective
To evaluate the outcome of ovarian stimulation and in vitro fertilization (IVF) in women undergoing fertility preservation prior to chemotherapy compared with healthy patients with infertility due to tubal factor.Study design
Case control, retrospective study in an academic IVF unit. The study participants were 21 cancer patients and 1 patient with focal proliferative glomerulosclerosis, undergoing emergency IVF or intracytoplasmic sperm injection (ICSI; Group A) and 22 patients undergoing IVF for tubal factor (Group B). All patients underwent controlled ovarian stimulation, ovum pick-up, and embryo freezing or transfer. The outcome measures included: dose of gonadotropins, mean estradiol and progesterone levels, length of stimulation, number of retrieved oocytes, number of 2 pronuclei zygotes, fertilization rate, and clinical pregnancy rate. Student's t-test was used for assessment of group comparisons.Results
Patients in Group A (mean age 32.8 ± 5.7 years) underwent 22 emergency IVF cycles for fertility preservation prior to chemotherapy. The mean number of days until human chorionic gonadotropin administration was 10.4 ± 4.8. Eleven cycles involved normal insemination while nine involved ICSI. In one cycle three arrested immature oocytes were retrieved, and in one cycle no oocytes were retrieved. Donor sperm was used in 9 cycles. Tamoxifen was part of the treatment protocol in 6 IVF cycles of breast cancer patients. The mean age of the women in Group B was 34 ± 4.2 years. There were no significant differences in any of the main outcome measures between the two groups. Thawed embryos were transferred in four cancer patients: two patients had colon cancer, one had breast cancer and one had pseudomyxoma peritonei. Two of these four women conceived and gave birth to healthy newborns.Conclusions
Emergency IVF is a promising approach for preserving fertility in cancer patients. Current treatment protocols offer a minimal time delay until chemotherapy is commenced, and the ovarian stimulation outcomes are comparable to those of women with tubal factor. 相似文献17.
Manizheh Sayyah-Melli Mohammad-Reza Rashidi Mohaddeseh Kaseb-Ganeh Nadereh Rashtchizadeh Simin Taghavi Elaheh Ouladsahebmadarek Morteza Ghojazadeh 《European journal of obstetrics, gynecology, and reproductive biology》2012
Objective
To determine the effect of recombinant erythropoietin on serum oxidants and the viability of ischemic ovaries after detorsion.Study design
A non-randomized single-blind clinical trial was conducted from December 2009 to January 2011 in a University Teaching Hospital affiliated with the School of Medicine, Tabriz University of Medical Sciences. Surgery was carried out on 40 patients, aged 18–35 years, with signs and symptoms of ovarian torsion. The patients were divided into two equal groups: group 1 received recombinant erythropoietin 150 IU/kg subcutaneously during the operation and 72 h after detorsion, and group 2 received no medication. Blood samples were taken before and 72 h after detorsion to determine the plasma levels of malondialdehyde, xanthine oxidase, glutathione, superoxide dismutase, nitric oxide, and total antioxidants. In both groups, the arterial and venous blood supply of the ovary and arterial blood flow resistance were evaluated before surgery and 72 h after detorsion of the ovary. The main outcome measures were improving ovarian blood flow and reducing oxidative damage. SPSS 17.0 was used for statistical analyses.Results
The levels of malondialdehyde, glutathione, superoxide dismutase, nitric oxide, and total antioxidants 72 h after detorsion were significantly different between the interventional and non-interventional groups (p < 0.001). There was no significant difference in the levels of xanthine oxidase (p = 0.13). The difference between groups in the blood flow of the ovary 72 h after surgery was not statistically significant (p = 0.61).Conclusion
Recombinant erythropoietin was effective in reducing the oxidative damage of ovarian torsion. 相似文献18.
Sezai Sahmay Nil AtakulMahmut Oncul Abdullah TutenBegum Aydogan Hakan Seyisoglu 《European journal of obstetrics, gynecology, and reproductive biology》2013
Objective
To characterize the difference in circulating anti-Müllerian hormone (AMH) levels between the main polycystic ovary syndrome (PCOS) phenotypic groups and evaluate the role of AMH in predicting the severity of PCOS.Study design
Cross-sectional, retrospective study. A total of 251 women were divided into four groups based on the main features of PCOS, as follows: Group 1 (polycystic ovarian morphology [PCOM]+/oligo-anovulation [OA]+/hyperandrogenism [HA]+), Group 2 (PCOM+/OA+/HA−), Group 3 (PCOM+/OA−/HA+), and Group 4 (PCOM−/OA+/HA+). AMH and other hormone levels were measured in serum. The main outcome was serum AMH concentrations in the main phenotypes of PCOS.Result(s)
The mean serum AMH levels were 9.50 ± 6.1 ng/mL in Group 1; 8.02 ± 6.2 ng/mL in Group 2; 6.12 ± 3.6 ng/mL in Group 3; and 3.06 ± 2.4 ng/mL in Group 4. Circulating AMH levels in Group 1 (PCOM+/OA+/HA+) were three times higher than those in Group 4 (PCOM−/OA+/HA+).Conclusions
The highest AMH levels were found in cases where all three main diagnostic criteria existed. AMH levels correlate best with PCOM. In addition, oligo-anovulation contributes to increased AMH levels. Hyperandrogenism criteria were found to have less influence on AMH levels. AMH levels seem to have a diagnostic role in determining the severity of PCOS. 相似文献19.
Hai-Ying Wang Song Quan Ren-Li Zhang Hai-yan Ye Yan-ling Bi Zhou-mei Jiang Ernest H.Y. Ng 《European journal of obstetrics, gynecology, and reproductive biology》2013
Objective
To compare serum anti-Mullerian hormone (AMH) levels following hysterectomy and myomectomy.Study design
Prospective longitudinal observational study. Serum AMH, follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels were measured pre-operatively (T1) and 2 days (T2) and 3 months (T3) following hysterectomy and myomectomy in 70 women aged 36–45 years. Hysterectomy (laparoscopy-assisted vaginal hysterectomy = 10; total abdominal hysterectomy = 25) with conservation of both ovaries for benign diseases of the uterus was performed in 35 women, and myomectomy (laparoscopy myomectomy = 15; open myomectomy = 20) was performed in another 35 women. The follow-up period was 3 months following surgery. The results were analysed using the t-test or one-way analysis of variance by repeated-measures ANOVA.Results
Serum AMH in the hysterectomy group was 1.08 ± 0.77 ng/ml at T1, 0.78 ± 0.58 ng/ml at T2 and 0.81 ± 0.58 ng/ml at T3; the level was significantly lower at T2 and T3 compared with T1. In the myomectomy group, the corresponding values were 1.54 ± 0.95 ng/ml, 1.18 ± 0.77 ng/ml and 1.50 ± 0.58 ng/ml; serum AMH was significantly lower at T2 compared with T1, but the difference between T3 and T1 was not significant. There were no significant differences in serum FSH and LH in either group between these three time points.Conclusion
Serum AMH was significantly lower 2 days and 3 months following hysterectomy compared with the pre-operative level. Following myomectomy, serum AMH was significantly lower than the pre-operative level 2 days following the procedure, but was similar to the pre-operative level 3 months after surgery. Therefore, hysterectomy may have a more lasting adverse effect on ovarian reserve than myomectomy. A long-term study of AMH levels is needed. 相似文献20.
Thomas Rutherford James Orr Jr. Edward Grendys Jr. Robert Edwards Thomas C. Krivak Robert Holloway Richard G. Moore Larry Puls Todd Tillmanns Julian C. Schink Stacey L. Brower Chunqiao Tian Thomas J. Herzog 《Gynecologic oncology》2013