共查询到20条相似文献,搜索用时 31 毫秒
1.
Yoni Cohen Alexandra St-Onge-St-Hilaire Samer Tannus Grace Younes Michael H. Dahan William Buckett Weon-Young Son 《Journal of assisted reproduction and genetics》2018,35(9):1683-1689
Purpose
To assess effects on fertilization rate, embryo quality, pregnancy, and live birth rates of vitrification and warming of oocytes that matured in vitro (vIVM) compared to fresh in vitro maturation (fIVM) cycles.Methods
A retrospective cohort study conducted at a university hospital-affiliated IVF unit. Fifty-six cycles of vIVM cycles and 263 fIVM in women diagnosed with polycystic ovarian syndrome (PCOS) ovaries were included in the analysis. The study group included PCOS patients who failed ovulation induction with intrauterine insemination and were offered IVM cycle followed by oocyte vitrification and warming. The embryological aspects and clinical outcomes were compared to those of controls undergoing fresh IVM cycles during the same period. The main outcome measure was live birth rate.Results
One thousand seventy oocytes were collected from 56 patients and underwent vitrification and warming. In the control group, 4781 oocytes were collected from 219 patients who had undergone a fresh IVM cycle. Oocyte maturation rates were similar between the groups (mean ± SD: 0.7?±?0.2 vs. 0.6?±?0.2, for vIVM and fIVM, respectively). Survival rate after warming was 59.8%. Fertilization and embryo cleavage rates per oocyte were significantly lower in the vIVM group. Clinical pregnancy (10.7 vs. 36.1%) and live birth rates (8.9 vs. 25.9%) per cycle were significantly lower in the vIVM group than those in the fIVM group (P?=?0.005 and P?<?0.001, respectively). Five healthy babies were born in the vIVM group.Conclusions
The reproductive potential of vitrified IVM oocytes is impaired. This injury likely occurs through vitrification and warming.2.
Farideh Yazdanpanah Mohammad Ali Khalili Maryam Eftekhar Hojatallah Karimi 《Archives of gynecology and obstetrics》2013,288(2):439-444
Background
15 % of oocytes collected from Assisted Reproductive Technology (ART) cycles are immature. These oocytes may be matured following in vitro maturation (IVM) program. It is possible to cryopreserve the immature oocytes for further use in ART after application of IVM.Objective
The aim was to determine the maturation rate and viability of human oocytes that were matured in vitro after vitrification program.Materials and methods
63 women (19–43 years old) who underwent controlled ovarian stimulation for ART were included in this study. 53 immature oocytes were used for fresh group (fIVM) and 50 immature oocytes for vitrification group (vIVM). The maturation medium was Ham’s F10 supplemented with 0.75 IU FSH, 0.75 IU LH and 40 % human follicular fluid (HFF). After 36 h, maturation and morphology of all oocytes were assessed. Also, the oocyte viability was assessed using PI/Hoechst immunostaining technique.Results
The maturation rates were reduced in vIVM group (56.0 %) in comparison to fIVM group (88.7 %; P < 0.001). Oocyte viability rate were also reduced in vIVM group (56.0 %) in comparison to fIVM (86.8 %, P < 0.007).Conclusions
Cryopreservation via vitrification reduced both the maturation capacity and viability of human oocytes in IVM technology. It is, therefore, recommended to apply IVM on fresh immature oocytes, instead. 相似文献3.
Joseph O. Doyle Ho Joon Lee Kaisa Selesniemi Aaron K. Styer Bo R. Rueda 《Journal of assisted reproduction and genetics》2014,31(12):1695-1702
Purpose
Investigate the effect of vitrification on in vitro maturation (IVM) and expression of Aurora kinases A, B, and C in germinal vesicle (GV)-stage oocytes.Methods
GV-stage oocytes from B6D2F1 female mice 7–11 weeks of age were vitrified after collection, thawed, and matured in vitro for 0, 4, 8, and 12 h (hrs). The rate of germinal vesicle breakdown (GVBD), spindle apparatus assembly, and Aurora kinase mRNA and protein expression during IVM was measured.Results
Oocyte vitrification was associated with significant delays in both GVBD and normal spindle apparatus assembly at 4 and 8 h of IVM (p < 0.05). There was no difference in mRNA levels between control and vitrified oocytes for any of the Aurora kinases. Aurora A protein levels were reduced in vitrified compared to control oocytes at 0 h (p = 0.008), and there was no difference at 4 and 8 h (p = 0.08 and 0.69, respectively) of IVM.Conclusions
Vitrified oocytes have delayed GVBD and normal spindle assembly during in vitro maturation. Reduced levels of Aurora A protein immediately post-thaw may be associated with the impaired oocyte maturation manifested by the delayed progression through meiosis I and II, and the atypical timing of the formation of meiotic spindles in vitrified GV-stage oocytes. 相似文献4.
Purpose
This study aims to determine if in-vitro maturation (IVM) of human immature oocytes should be performed before or after vitrification.Methods
A total of 184 immature oocytes were randomly divided into two different groups: 100 were vitrified at metaphase II (MII) stage 24 h-48 h after IVM (group 1) and 84 were immediately vitrified at germinal vesicle (GV) or metaphase I (MI) stages and in vitro matured after warming (group 2).Results
Survival rate after warming was similar in both groups (86.9% versus 84.5%). However, oocyte maturation rate per collected oocyte was significantly higher for oocytes matured before vitrification (group 1, 46%) than for oocytes vitrified before IVM (group 2, 23.8%) (p < 0.01). Consequently, the number of MII oocytes inseminated per oocyte collected was significantly higher for group 1 (40%) than for group 2 (23.8%) (p < 0.05).Conclusion
IVM procedure is more efficient when it is performed before oocyte vitrification. 相似文献5.
Elisa Melo Ferreira Alessandra Aparecida Vireque Paulo Roberto Adona Rui Alberto Ferriani Paula Andrea Navarro 《European journal of obstetrics, gynecology, and reproductive biology》2009
Objectives
Asynchrony between nuclear and cytoplasmic maturation, and possibly damage to the oocyte meiotic spindle, limits the application of in vitro maturation (IVM) in assisted reproduction. Several studies have suggested that Prematuration with meiosis blockers may improve oocyte quality after IVM, favoring early embryogenesis. Thus, we investigated the effect of Prematuration with the nuclear maturation inhibitor butyrolactone I (BLI) on the meiotic spindle and chromosomal configuration of bovine oocytes.Study design
Immature oocytes obtained from cows slaughtered in a slaughterhouse (n = 840) were divided into the following groups: (1) control (n = 325), submitted only to IVM in TCM199 for 24 h; (2) BLI 18 h (n = 208) submitted to meiotic blockage with 100 μM BLI for 24 h (Prematuration) and then induction of IVM in TCM199 for 18 h; and (3) BLI 24 h (n = 307), pre-matured with 100 μM BLI for 24 h followed by 24 h of IVM in TCM199. The oocytes were then fixed, stained by immunofluorescence for morphological visualization of both microtubules and chromatin, and evaluated.Results
Meiotic arrest occurred in 90.2% of the oocytes cultured with BLI. Maturation rates were similar for all groups (80.3%, 73.6% and 82.7% for the control, BLI 18 h and BLI 24 h groups, respectively). We observed 81.3% normal oocytes in metaphase II in the control group, and 80.0% and 81.2% in the BLI 18 h and BLI 24 h groups, respectively. The incidence of meiotic anomalies did not differ between groups (18.7%, 20.0% and 18.8% for the control, BLI 18 h and BLI 24 h, respectively).Conclusion
Prematuration with butyrolactone I reversibly arrests meiosis without damaging the meiotic spindle or the chromosome distribution of bovine oocytes after in vitro maturation. 相似文献6.
Xiaoyun Shi Shiling Chen Haiyan Zheng Lele Wang Yaqin Wu 《European journal of obstetrics, gynecology, and reproductive biology》2013
Objective
To evaluate the epigenetic risk linked to assisted reproductive technology at oocyte level by analyzing methylation status of imprinted H19, PEG1 and KvDMR1 in human MII oocytes rescue-matured from MI/GV oocytes after long agonist stimulation.Study design
580 MI/GV oocytes from 275 patients receiving an ICSI procedure were additionally cultured for 24 h, and 221 rescue-matured MII oocytes were obtained. Pyrosequencing with confirmatory routine bisulfite sequencing were used to determine the methylation status of H19 DMR in 35 oocytes, PEG1 DMR in 47 oocytes, and KvDMR1 in 34 oocytes.Results
Abnormal methylation status were found in 22.9% oocytes at H19 DMR, 17.0% oocytes at PEG1 DMR and 8.8% oocytes at KvDMR1.Conclusion
We hypothesize that the use of MII-rescue oocytes may increase the risk of imprinting defects because they might not have completed full imprinting programme. 相似文献7.
Georg Griesinger Henriette BerndtLaura Schultz Marion DepenbuschAskan Schultze-Mosgau 《European journal of obstetrics, gynecology, and reproductive biology》2010
Objectives
To prospectively study the incidence of OHSS, live birth likelihood and neonatal outcome after GnRH-agonist triggering of final oocyte maturation and vitrification of all pronucleate (2PN) oocytes for later frozen–thawed embryo transfer (FRET) in an OHSS-risk population.Study design
Prospective, clinical cohort study (12/2004–5/2009). Forty patients undergoing ovarian stimulation in a GnRH-antagonist protocol and at risk of developing severe OHSS underwent triggering with 0.2 mg triptorelin and elective vitrification of all 2PN-oocytes for later frozen–thawed embryo transfer.Results
The incidence of OHSS was 0% (0/40; 95% confidence interval: 0.0–6.4%). Thirty-nine patients underwent 87 FRETs (mean number of FRETs per patient: 2.2 ± 1.6; range: 1–7). The cumulative live birth rate per patient was 35.0% (14/40; 95% confidence interval: 23.9–48.0%). Mean time-to-conception resulting in live birth after agonist triggering was 24.2 (±17.1; range: 9–67) weeks. Nine healthy singletons and five twins were born.Conclusions
A treatment algorithm combining agonist trigger with vitrification of all 2PN-oocytes is feasible and safe, and provides patients with a good cumulative chance of live birth. 相似文献8.
9.
Jordana H. Hyman Ehud J. Margalioth Ron Rabinowitz Avi Tsafrir Michael Gal Sarah Alerhand Nurit Algur Talia Eldar-Geva 《European journal of obstetrics, gynecology, and reproductive biology》2013
Objective
Dehydroepiandrosterone (DHEA) supplementation for poor responders may improve ovarian response and IVF treatment outcome. This study aimed to determine the mechanism of action of DHEA, and specifically, the stage of folliculogenesis influenced by DHEA.Study design
This is a prospective, self-controlled study of poor responders to IVF treatment, comparing day 3 biochemical (anti-Mullerian hormone (AMH), inhibin B and FSH) and ultrasound (antral follicle count (AFC)) ovarian reserve markers and IVF treatment outcome before and after DHEA supplementation of at least 3 months duration.Results
Thirty-two women were included. Following DHEA, there was a significant increase in AFC (P = 0.0003) without significant changes in the baseline biochemical parameters AMH, inhibin B, or FSH. The enhanced response comprised increased peak estradiol levels (P = 0.0005), number of follicles > 15 mm, oocytes, MII oocytes and embryos (P = 0.004, P = 0.00001, P = 0.0004 and P = 0.0006, respectively) and oocytes number/total FSH dose (P = 0.0009). The proportion of cancelled cycles due to very poor response decreased significantly (P = 0.02).Conclusions
DHEA does not appear to exert influence via recruitment of pre-antral or very small antral follicles (no change in AMH and inhibin B) but rather by rescue from atresia of small antral follicles (increased AFC). 相似文献10.
Antoine Hannoun Anwar H. NassarIhab M. Usta Antoine Abu Musa 《European journal of obstetrics, gynecology, and reproductive biology》2010
Objective
Smoking is a significant health hazard that has been associated with poor reproductive outcome and reduced fertility in reproductive age women. The aim of this study was to assess the effect of nargile smoking on intra-cytoplasmic sperm injection (ICSI) outcome.Study design
A prospective analysis of the outcomes of 297 women who underwent ICSI treatment at the ART Unit at the American University of Beirut Medical Center between January 1, and December 31, 2006 was done. The patients were divided into 3 groups based on their smoking status: cigarette smokers (n = 42), nargile smokers (n = 51) and non-smokers (n = 204).Results
The mean age of nargile smokers was significantly lower than the other groups; however, the 3 groups were similar with respect to the cause of infertility, total dose of follicular stimulating hormone (FSH), number of oocytes and embryos obtained, and number and quality of embryos transferred. There was no significant difference in the clinical pregnancy rate between nargile smokers and non-smokers (51.0% vs 43.6%). However, cigarette smokers had a significantly lower clinical pregnancy rate compared to non-smokers (23.8% vs 43.6%, p = 0.0238). On multiple logistic regression analysis, factors that decreased the clinical pregnancy rates were cigarette smoking and maternal age.Conclusion
Although this study did not find a deleterious effect of nargile smoking on ICSI outcome, the results need to be confirmed in prospective studies that would include larger number of women with more objective measures of nargile smoke exposure. 相似文献11.
Duo Wei Culian Zhang Juanke Xie Xiaobing Song Baoli Yin Qi Liu Lin Hu Haoying Hao Jiaxuan Geng Peng Wang 《Journal of assisted reproduction and genetics》2013,30(7):933-938
Purpose
Studies in bovine and porcine have indicated that melatonin (MT) could induce meiotic maturation of immature oocytes in vitro. The object of the current study was to investigate if MT could ameliorate human oocytes maturation during rescue in vitro maturation (IVM).Methods
Two hundred seventy eight germinal vesicle (GV) oocytes and 451 (MI) metaphase I oocytes were vitrified, thawed and then matured in vitro. All the oocytes were randomly allocated into six groups in which the oocytes were cultured in medium supplemented with different concentrations of MT (0, 10−2, 1, 102, 104, 106 nM) and nuclear maturation was evaluated at 6 h, 12 h, 18 h, 24 h and 48 h of culture.Results
The optimal MT concentration for both GV and MI oocytes was 1 nM. At 24 h of culture, nuclear maturation rate of MI oocytes cultured in 1 nM MT medium was significantly higher than other groups (P < 0.05); Nuclear maturation rate of GV oocytes cultured in 1 nM MT medium was also significantly higher than the control group (P < 0.05). On the other hand, decreased nuclear maturation rate was observed in the high MT concentration group (106 nM).Conclusions
The current study demonstrated that low concentration of exogenous MT could ameliorate nuclear maturation of human oocyte during rescue IVM, while high concentration of MT presented negative effects. 相似文献12.
Einat Shalom-Paz Hananel Holzer Weon- Young Son Ishai Levin Seang Lin Tan Benny Almog 《European journal of obstetrics, gynecology, and reproductive biology》2012
Objective
Our aim was to compare treatment outcome following in vitro maturation (IVM) compared with IVF in patients with polycystic ovarian syndrome (PCOS).Study design
Retrospective evaluation of treatment in women with PCOS who underwent IVM (108) and IVF (108).Results
We found a significant difference in outcome between IVM and IVF, with an increase in the number of mature oocytes derived (10.5 ± 6.5 vs. 15.3 ± 8.8, p < 0.0001) and the cleavage rate (92.4 ± 13.0 vs. 95.2 ± 11.7, p = 0.03) in IVM cycles. Due to the lower implantation rate (16.1% vs. 21.6%, p = 0.07) we tend to transfer more embryos in the IVM group (3.4 ± 0.8 vs. 2.8 ± 1.0, p < 0.0001), but the multiple pregnancy rate in that group was not higher. Importantly, the delivery rate was similar in both groups (26.8% vs. 25%). We also report a yearly change in the success rate of IVM during this period.Conclusions
IVM treatment for PCOS patients may be a valid alternative treatment to IVF with the advantage of eliminating the risk of OHSS and reducing the cost of medication, whilst maintaining high clinical pregnancy rate. 相似文献13.
Gurkan Kiran Yakup Gumusalan Hasan C. Ekerbicer Hakan Kiran Ayhan Coskun Deniz C. Arikan 《European journal of obstetrics, gynecology, and reproductive biology》2013
Objective
To compare the therapeutic effect of acupuncture and non-steroidal anti-inflammatory drug (NSAID) therapy in primary dysmenorrhea patients.Study design
Thirty-five young women with a diagnosis of primary dysmenorrhea were recruited for the study. Their dysmenorrhea severity was rated by visual analog scale (VAS) immediately prior to entry into the study. They were randomly divided into two groups; and the following month they were given NSAID (group 1, n = 24) or acupuncture treatment (group 2, n = 11). Pain was rated again using VAS during menstruation in both groups.Results
After one month's treatment, pain scores were significantly lower in both groups (p < 0.05). Mean pain scores decreased by 52.2% and 69.5% in the NSAID and acupuncture groups, respectively.Conclusion
Acupuncture was as effective as NSAID therapy for patients with primary dysmenorrhea. Since this was a pilot study with a small sample size and short follow-up period, larger studies are needed to clarify the effect of acupuncture in the treatment of primary dysmenorrhea. 相似文献14.
Davide Gentilini Paola Vigano Davide Castaldi Daniela Mari Mauro Busacca Paolo Vercellini Edgardo Somigliana Anna Maria di Blasio 《European journal of obstetrics, gynecology, and reproductive biology》2009
Objectives
Some controversy exists for the potential association of the plasminogen activator inhibitor-1 (PAI-1) gene polymorphism 4G/5G and susceptibility to endometriosis. To clarify this issue, we have examined the prevalence of this polymorphism in a case–control study in the Italian population.Study design
The PAI-1 4G/5G polymorphism was evaluated in n = 368 reproductive year aged Caucasian women who underwent gynaecological laparoscopy for chronic pelvic pain, infertility, ovarian cysts and myomas. A second group of controls included n = 329 normal subjects.Results
The 697 women enrolled were divided as follows: the endometriosis group (n = 204), the gynaecological control group (n = 164) and the general population control group (n = 329). No statistical significant differences emerged between endometriosis patients and gynaecological controls with regard to the allele frequencies and co-dominant and dominant models of genotype distribution. A borderline statistical difference was only observed for the recessive model of inheritance in which, contrary to previous findings, the PAI-1 4G/4G genotype seems to be less linked to the disease development.Conclusion
The findings reported herein do not support the previously reported data indicating a greater susceptibility to endometriosis in patients harbouring the PAI-1 4G/5G and 4G/4G genotypes and exclude a significant role of polymorphism in endometriosis development. 相似文献15.
Sarah D. McDonald Mark C. Walker Arne Ohlsson Kellie E. Murphy Joseph Beyene Sherry L. Perkins 《European journal of obstetrics, gynecology, and reproductive biology》2008
Objective
To determine the effect of smoking on maternal and fetal thyroid function.Study design
This prospective cohort study involved healthy women undergoing elective cesarean section for term singleton infants. Maternal and fetal thyroid indices were compared between smokers (n = 21) and non-smokers (n = 83).Results
Maternal thyrotropin (TSH) concentrations were significantly lower in smokers than non-smokers (1.75 mIU/L versus 2.15 mIU/L, respectively, p = 0.007), with similar free thyroxine (FT4) concentrations (9.59 pmol/L versus 9.56 pmol/L, p = 0.755). For women who smoked, the correlation between the average number of cigarettes smoked per day and maternal TSH concentrations was −0.427, p = 0.054. Infants of smokers and non-smokers had similar umbilical TSH (5.43 mIU/L versus 5.82 mIU/L, p = 0.124) and FT4 concentrations (13.06 pmol/L versus 13.57 pmol/L, p = 0.049).Conclusion
We demonstrated for the first time that women who smoke during pregnancy have significantly lower TSH concentrations than non-smokers. 相似文献16.
Grzegorz Polak Iwona WertelBartłomiej Barczyński Wojciech KwaśniewskiWiesława Bednarek Jan Kotarski 《European journal of obstetrics, gynecology, and reproductive biology》2013
Objective
To evaluate 8-hydroxy-2-deoxyguanosine (8-OHdG) and 8-isoprostane levels in the peritoneal fluid (PF) of women with endometriosis.Study design
One hundred and ten women with laparoscopically and histopathologically confirmed endometriosis and, as reference groups, 119 patients with simple serous (n = 78) and dermoid (n = 41) ovarian cysts were studied. Peritoneal fluid 8-OHdG and 8-isoprostane concentrations were evaluated by enzyme-linked immunosorbent assays.Results
8-OHdG and 8-isoprostane levels in peritoneal fluid were significantly higher in patients with endometriosis compared with the reference groups. Higher PF 8-OHdG and 8-isoprostane concentrations were observed in patients with advanced stages of endometriosis. A statistically significant positive correlation was found between 8-OHdG and 8-isoprostane levels in peritoneal fluid.Conclusion
Endometriosis induces greater oxidative stress and frequent DNA mutations in peritoneal fluid than nonendometriotic ovarian cysts. The most severe oxidative stress occurs in the peritoneal cavity of women with more advanced stages of the disease. 相似文献17.
Sung-Woo Choi Bon-Hee Gu Suresh Ramakrishna Jung-Mi Park Kwang-Hyun Baek 《European journal of obstetrics, gynecology, and reproductive biology》2009
Objective
The aim of the present study was to investigate whether there is an association between the C677T polymorphism in MTHFR and PCOS in a Korean population.Study design
The prevalence of MTHFR gene was compared between women with PCOS (n = 227) and normal patients (n = 115) using restriction fragment length polymorphism (RFLP) analysis. The HapAnalyzer was used to analyze the genotype of MTHFR polymorphism in PCOS and control subjects. We considered a p-value less than 0.05 as statistically significant.Results
The frequency of C/C, C/T, and T/T genotype showed similar proportion between PCOS and control subjects. In addition, the frequencies of co-dominant (p-value = 0.8334, odds ratio (OR) = 1.04), dominant (p-value = 0.8749, OR = 0.96) and recessive alleles (p-value = 0.5574, OR = 1.22) did not show any association between PCOS and control subjects.Conclusion
Our data demonstrate that the C677T polymorphism of MTHFR gene is not associated with PCOS in a Korean population, suggesting that the C677T polymorphism in MTHFR may have different influences in various ethnic groups and diseases. 相似文献18.
Kathy Whyte Hannah KellyVicky O’Dwyer Michelle GibbsAmy O’Higgins Michael J. Turner 《European journal of obstetrics, gynecology, and reproductive biology》2013
Objectives
Maternal lipid metabolism is altered during pregnancy but little is known about the influence of these alterations on either intrauterine fetal development or maternal wellbeing. The purpose of this study was to examine the relationship between both fasting cholesterol and triglycerides and offspring birth weight in women screened selectively for gestational diabetes mellitus (GDM).Study design
In a prospective observational study in a University Maternity Hospital, women were recruited at their convenience when they were screened for GDM with a diagnostic 75 g oral glucose tolerance test (OGTT). An additional sample was taken for a lipid profile at the time the fasting glucose was obtained. Clinical and socio-demographic details were recorded.Results
Of the 189 women recruited, the mean age was 32 years, 35.4% (n = 67) were primigravidas, 44.1% (n = 82) were obese and 11.6% (n = 22) had an abnormal OGTT. On univariate analysis, increasing birth weight was correlated positively with multiparity, first trimester body mass index (BMI), GDM and hypertriglyceridaemia but not with cholesterol levels. On multivariate analysis, increased birth weight correlated positively only with hypertriglyceridaemia.Conclusions
This study provides further evidence that maternal hypertriglyceridaemia is important in programming intrauterine fetal growth and raises questions about whether women should be screened selectively for dyslipidaemia before, during and after pregnancy. 相似文献19.
Objective
To study the role of a phyto-oestrogen, Cimicifuga racimosa extract (Klimadynon®, Bionorica, Neumarkt i.d.OBf., Germany), in ovulation induction in women with polycystic ovarian syndrome (PCOS).Study design
Prospective randomized controlled trial in Minia University Hospital, Minia, Egypt. One hundred women with PCOS were allocated into one of two groups: one group (n = 50) received clomiphene citrate 100 mg daily for 5 days, and the other group (n = 50) received C. racimosa 20 mg daily for 10 days. Both groups received medication starting from the second day of the cycle for three consecutive cycles, during which changes in follicle-stimulating hormone (FSH), luteinizing hormone (LH), FSH/LH ratio, progesterone, endometrial thickness and pregnancy rate were measured.Results
The groups were similar in terms of age, clinical presentation and hormonal levels before treatment. Following treatment, significant favourable changes in LH level and FSH/LH ratio (p = 0.007 and 0.06, respectively) were seen in the Klimadynon group. In this group the progesterone level was higher from the first treatment cycle, indicating better ovulation (p = 0.0001), and endometrial thickness was greater (p = 0.0004). The pregnancy rate was higher in the Klimadynon group but the difference between the groups was not significant (p = 0.1).Conclusion
Phyto-oestrogen can be used as an alternative to clomiphene citrate for ovulation induction in women with polycystic ovarian syndrome. 相似文献20.
Jung-Yun Lee Jina Youm Tae Hun Kim Jeong Yeon Cho Min A Kim Dong Hoon Suh Myong Cheol Lim Jae-Weon Kim Noh Hyun Park Yong-Sang Song 《Gynecologic oncology》2014