加味左金丸对大鼠胃癌前病变环氧合酶-2蛋白表达的影响

目的观察加味左金丸对大鼠胃癌前病变环氧合酶-2(COX-2)蛋白表达的影响,并探讨其治疗机理。方法采用MNNG、0.03%雷尼替丁、56℃15%盐水、0.85%脱氧胆酸钠、40%乙醇、饥饱失常等多种因素制作大鼠胃癌前病变模型和免疫组化方法检测正常组、自然恢复组、加味左金丸高、中、低剂量组、维甲酸组大鼠胃粘膜组织中COX-2蛋白表达的情况。结果加味左金丸中、低剂量组COX-2蛋白染色积分与自然恢复组比较差异有统计学意义(P<0.01,P<0.05)),高剂量组和维甲酸组与自然恢复组比较差异无统计学意义(P>0.05);加味左金丸中剂量组COX-2蛋白染色积分与维甲酸组比较差异有统计学意义(P<0.01)。结论加味左金丸对COX-2蛋白的高表达有明显的抑制作用,其治疗胃癌前病变获效的机制之一可能与其在一定程度上通过调控多种癌基因、抑癌基因,降低COX-2蛋白的表达,进而恢复细胞增殖与凋亡的平衡有关。     

丙烯酰胺对大鼠睾丸的毒性作用及其对增殖细胞核抗原蛋白表达的影响

目的 观察丙烯酰胺(AA)对大鼠睾丸的毒性作用及其对增殖细胞核抗原(PCNA)蛋白表达的影响.方法 将40只雄性SD大鼠随机分为对照组(双蒸水)、4 mg/kg AA染毒组、12 mg/kg AA染毒组和36 mg/kg AA染毒组.连续灌胃30 d后,取睾丸行苏木精-伊红染色,观察睾丸组织形态学变化;TUNEL法检测睾丸细胞凋亡情况;化学发光法检测血清中睾酮和雌二醇含量;反转录聚合酶链反应(RT-PCR)检测睾丸组织PCNA mRNA表达;免疫组织化学法和Western blotting技术检测睾丸组织PCNA蛋白表达.结果 12 mg/kg和36 mg/kg AA染毒组大鼠体质量明显低于对照组(P＜0.05).12 mg/kg和36 mg/kg AA染毒组大鼠睾丸组织生精细胞减少,生精上皮萎缩,管腔出现空泡,凋亡细胞显著增多.各染毒组大鼠血清睾酮水平均显著低于对照组(P＜0.05);12 mg/kg和36 mg/kg AA染毒组大鼠血清雌二醇水平显著低于对照组(P＜0.05).与对照组比较,12 mg/kg和36 mg/kg AA染毒组大鼠睾丸组织PCNA mRNA和蛋白表达均明显降低(P＜0.05).结论 AA染毒可引起雄性大鼠性激素分泌减少,睾丸组织发生病理学改变,凋亡细胞增多,PCNA蛋白表达减少,打乱生精细胞增殖与凋亡的动态平衡,导致生殖系统发生不同程度的功能障碍.     

茶多酚对胃黏膜缺血再灌注损伤的保护作用

目的 观察茶多酚(polyphenols of tea,PPT)对因胃缺血致胃黏膜损伤的保护作用.方法 将35只雄性SD大鼠随机分成7组A空白对照组;B假手术组;C手术组;D 20 mg/kg PPT组;E 40 mg/kg PPT组;F 80 mg/kg PPT组;G 160 mg/kg PPT组.在成功制作胃缺血动物模型的基础上,观察大鼠胃缺血30 min再灌注后1 h,2 h,6 h,12 h大鼠血浆中超氧物歧化酶(SOD)、丙二醛(MDA)水平的变化,同步观察再灌注12 h后胃黏膜内毛细血管内皮中血管内皮生长因子(VEGF)和CD34的表达变化.并设立手术组、假手术组以及空白对照组予以对照.结果 各模型组相应时间点的SOD含量明显高于手术组、假手术组和空白对照组(P＜0.05),其中40 mg/kg组最为显著(P＜0.01);20 mg/kg,40 mg/kg,80 mg/kg组相应时间点的MDA含量明显低于手术组、假手术组和空白对照组(P＜0.05),其中80 mg/kg组最为显著(P＜0.01),160 mg/kg组则略低于手术组、高于假手术组和空白对照组(P＞0.05);各模型组的胃黏膜内毛细血管内皮中CD34表达水平明显高于手术组(P＜0.05),略低于假手术组和空白对照组(P＞0.05);各模型组的胃黏膜内毛细血管内皮中VEGF表达水平明显低于手术组(P＜0.05),略高于假手术组和空白对照组(P＞0.05).结论 茶多酚对因缺血再灌注导致损伤的胃黏膜具有保护作用.     

茯苓酸调节PI3K/Akt信号通路对心力衰竭大鼠心肌纤维化的影响

目的 观察茯苓酸(PA)调节磷脂酰肌醇3-激酶(PI3K)/丝苏氨酸蛋白激酶B(Akt)信号通路对心力衰竭(HF)大鼠心肌纤维化(MF)的影响。方法 将108只大鼠随机分为6组,分别为假手术组、模型组、茯苓酸低剂量组(1 mg/kg)、茯苓酸中剂量组(5mg/kg)、茯苓酸高剂量组(10 mg/kg)、激活剂组(10 mg/kg PA+0.02 mg/kg PI3K激活剂740Y-P),每组18只。采用左冠状动脉前降支结扎法诱导心肌梗死(MI)后HF大鼠造模成功后用超声影像系统检测大鼠心功能;HE染色观察大鼠心肌组织形态;Masson染色观察大鼠心肌胶原蛋白的分布并计算胶原容积分数(CVF);羟脯氨酸(HYP)试剂盒测定大鼠心肌组织HYP含量;TUNEL染色检测大鼠心肌细胞的凋亡情况;检测大鼠心肌组织活性氧(ROS)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平;ELISA法检测各组大鼠心肌组织白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平;Western Blot检测心肌组织Bcl-2相关X蛋白(Bax)、半胱氨酸蛋白酶-3(Caspase-3)...     

不同剂量托吡酯对癫大鼠海马组织中NCAM和GAP- 43 mRNA表达的影响

目的 研究不同剂量托吡酯(TPM)干预下,大鼠海马组织中神经细胞黏附分子(NCAM)和生长相关蛋白- 43(GAP- 43)mRNA的表达变化.方法 将48只大鼠随机分成正常对照组、海人酸(KA)组、10 mg/kg TPM组、40 mg/kg TPM组、100 mg/kg TPM组和400 mg/kg TPM组,每组8只;建立不同剂量的TPM干预癫大鼠模型.观察大鼠行为学改变;通过Real-time PCR测定各组大鼠海马组织中NCAM和GAP- 43的mRNA表达.结果 KA组大鼠海马NCAM及GAP- 43的mRNA表达水平明显高于正常对照组(P<0.01),10 mg/kg TPM组和40 mg/kg TPM组与KA组的差异无统计学意义,100 mg/kg TPM组和400 mg/kg TPM组明显低于KA组(P<0.01),其中400 mg/kg TPM组明显低于100 mg/kg TPM组(P<0.01).结论 KA诱导癫大鼠海马NCAM和GAP- 43 mRNA表达上调,较大剂量TPM能够抑制NCAM和GAP- 43 mRNA的表达,且抑制作用与TPM剂量有关.     

不同剂量托吡酯对癫癎大鼠海马组织中NCAM和GAP-43 mRNA表达的影响

目的 研究不同剂量托吡酯(TPM)干预下,大鼠海马组织中神经细胞黏附分子(NCAM)和生长相关蛋白- 43(GAP- 43)mRNA的表达变化.方法 将48只大鼠随机分成正常对照组、海人酸(KA)组、10 mg/kg TPM组、40 mg/kg TPM组、100 mg/kg TPM组和400 mg/kg TPM组,每组8只;建立不同剂量的TPM干预癫大鼠模型.观察大鼠行为学改变;通过Real-time PCR测定各组大鼠海马组织中NCAM和GAP- 43的mRNA表达.结果 KA组大鼠海马NCAM及GAP- 43的mRNA表达水平明显高于正常对照组(P<0.01),10 mg/kg TPM组和40 mg/kg TPM组与KA组的差异无统计学意义,100 mg/kg TPM组和400 mg/kg TPM组明显低于KA组(P<0.01),其中400 mg/kg TPM组明显低于100 mg/kg TPM组(P<0.01).结论 KA诱导癫大鼠海马NCAM和GAP- 43 mRNA表达上调,较大剂量TPM能够抑制NCAM和GAP- 43 mRNA的表达,且抑制作用与TPM剂量有关.     

葛根素和丹参酮ⅡA组分配伍对糖尿病肾病大鼠肾组织P27~(kip1)蛋白的调控作用

目的探讨葛根素、丹参酮ⅡA组分配伍对糖尿病肾病大鼠p27kip1表达的影响,为中医药治疗糖尿病肾病提供实验依据。方法单次腹腔注射链脲佐菌素(65 mg/kg)建立糖尿病肾病大鼠模型,实验分为对照组、模型组、葛根素低、高剂量组(50 mg/kg,200 mg/kg)、丹参酮ⅡA低、高剂量组(10 mg/kg,40 mg/kg)、葛根素+丹参酮ⅡA低、高剂量组(葛根素50 mg/kg+丹参酮ⅡA 10 mg/kg,葛根素200 mg/kg+丹参酮ⅡA 40 mg/kg),8周末处死大鼠,测定血糖、24 h尿量及24 h尿蛋白,通过RT-PCR和Western blot法检测各组大鼠肾组织p27kip1mRNA及蛋白的表达。结果葛根素+丹参酮ⅡA高剂量组可明显降低糖尿病肾病大鼠血糖,减少尿量,减轻尿蛋白排泄,抑制糖尿病肾病大鼠肾组织P27~(kip1)蛋白表达(P0.05,P0.01),且优于其他各组。结论葛根素和丹参酮ⅡA组分配伍具有抗糖尿病肾病的作用,其机制可能是通过下调糖尿病肾病大鼠肾组织p27kip1的表达实现的。     

健胃消胀片通过调节PI3K-Akt-eNOS通路改善大鼠胃癌前病变

【目的】探讨健胃消胀片对大鼠胃癌前病变的治疗作用及机制。【方法】将40只雄性SD大鼠随机分为正常组、模型组、叶酸组和健胃消胀片组,每组10只。除正常组,其他3组大鼠采用雷尼替丁水溶液灌胃联合N-甲基-N’-硝基-N-亚硝基胍（MNNG）溶液饮用法制备胃癌前病变模型。成功造模后,相应给药治疗7周。记录造模及给药期间大鼠体质量变化,观察胃部大体观并进行病理评分,测定脾脏、肝脏系数,采用苏木素-伊红（HE）染色法观察胃组织病理形态变化,酶联免疫吸附分析（ELISA）检测血清胃泌素（GAS）、胃动素（MTL）、胰高血糖素（GC）含量,阿利新蓝-过碘酸雪夫氏（AB-PAS）染色法观察胃组织黏膜层厚度,蛋白免疫印迹（Western Blot）法检测胃组织磷脂酰肌醇3-激酶（PI3K）、磷酸化PI3K(p-PI3K)、蛋白激酶B(Akt)、磷酸化Akt(p-Akt)、内皮型一氧化氮合酶（eNOS）蛋白的表达,免疫荧光染色法检测胃组织血管内皮细胞生长因子A(VEGFA)蛋白的表达。【结果】与正常组比较,模型组大鼠实验期间体质量增长慢,胃部大体观病理评分显著升高（P<0.01）,脾脏系数和肝脏系数...     

丙泊酚对睡眠剥夺大鼠甲状腺PI3K/Akt通路及细胞凋亡的影响

目的 探讨丙泊酚对睡眠剥夺(SD)大鼠甲状腺磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(Akt)通路及细胞凋亡的影响.方法 Sprague Dawley大鼠50只分为正常组(Normal组)、SD模型组(SD组)、丙泊酚低剂量组(10mg/kg)、丙泊酚中剂量组(25mg/kg)、丙泊酚高剂量组(50mg/kg),每组10只.除Normal组模拟正常睡眠外,其余各组大鼠均建立SD模型,造模成功后丙泊酚各组腹腔注射相应剂量药物,Normal组和SD组腹腔注射等量生理盐水.观察各组大鼠毛色、行为等一般行为状况,称取大鼠体重,检测血清游离三碘甲腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(TSH)水平;取甲状腺组织称重,HE染色观察甲状腺病理变化;TUNEL染色观察甲状腺组织细胞凋亡;Western blot检测甲状腺组织PI3K、p-Akt、半胱氨酸天冬氨酸蛋白酶3(caspase-3)、B细胞白血病2(Bcl-2)蛋白表达水平.结果 与Normal组相比,SD组大鼠出现甲状腺组织滤泡腔减小、高度增生等病理损伤,体重及甲状腺重量、血清FT4水平、甲状腺组织PI3K、p-Akt、Bcl-2蛋白表达水平降低(P＜0.05),血清FT3、TSH水平、甲状腺细胞凋亡率、caspase-3蛋白表达水平升高(P＜0.05);与SD组相比,丙泊酚低、中、高剂量组大鼠甲状腺病理损伤减轻,体重、甲状腺重量、血清FT4水平及甲状腺组织PI3K、p-Akt、Bcl-2蛋白表达水平升高(P＜0.05),血清FT3、TSH水平、甲状腺细胞凋亡率、caspase-3蛋白表达水平降低(P＜0.05),且丙泊酚各剂量组上述指标呈剂量依赖性.结论 丙泊酚可激活SD大鼠甲状腺组织PI3K/Akt通路的蛋白表达,抑制甲状腺细胞凋亡和损伤,改善机体甲状腺激素分泌紊乱.     

吡格列酮对脂多糖所致大鼠海马损伤的保护作用及其对iNOS和IL-1β表达水平的影响

目的 研究过氧化体增殖物激活受体.y(PPARΥ)激动荆吡格列酮(pioglitazone,pio)对大鼠脑室注射脂多糖(lipopolysaccharide,LPS)所致海马损伤的保护作用及对诱导型一氧化氮合酶(Inducibh Nitric Oxide Syn-thase,iNOS)和白介素-1β(Intedeukin-1β,IL-1β)表达水平的影响.方法 雄性大鼠40只,随机分为4组,每组10只,即对照组,LPS模型组,LPS Hio40mg/kg组和LPS pio80mg/kg组.大鼠灌胃给予pio(40,80mg/kg)3周,脑室内注射LPS(5μl,30μg)1周后,取脑组织,制成石蜡切片,进行尼氏(Nissl)染色和胶质纤维酸蛋白(glial fibrillary acidic protein,GFAP)免疫组织化学染色.研究海马CA1区锥体神经元形态学改变和星形胶质细胞的活化、浸润情况.采用Western蛋白印迹检测方法观察IL-1β、iNOS的表达情况.结果 大鼠脑室内注射LPS可引起海马CA1区星形胶质细胞的激活和浸润及椎体神经元的损伤,同时伴有IL-1β,iNOS的表达增加.Pio(40、80mg/kg连续应用1周)能减轻海马CA1区椎体神经元的损伤及星形胶质细胞的激活和浸润,抑制鹏引起的IL-1β、iNOS表达增加.侧脑室注射LPS后1周,LPS组大鼠海马CAI区IL-1β蛋白表达水平较对照组差异有统计学意义(P＜0.01),LSP pio40mg/kg组和LSP Pio80mg,/kg组大鼠海马CAI区IL-1β表达量较LPS模型组差别有统计学意义(P＜0.01).LSP Pio40mg/kg组和LSP Pio80mg/kg组大鼠海马CA1区iNOS表达量较LPS模型组差别有统计学意义(P＜0.01).结论 Pio能够通过抑制LPS引起的IL-1β、iNOS的表达,减轻海马椎体神经元的损伤.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.