共查询到20条相似文献,搜索用时 19 毫秒
1.
Alonso-Castro AJ Ortiz-Sánchez E Domínguez F Arana-Argáez V Juárez-Vázquez Mdel C Chávez M Carranza-Álvarez C Gaspar-Ramírez O Espinosa-Reyes G López-Toledo G Ortiz-Andrade R García-Carrancá A 《Journal of ethnopharmacology》2012,141(3):888-894
Ethnopharmacological relevance
Medicinal plants are an important source of antitumor compounds. This study evaluated the acute toxicity in vitro and in vivo, as well as the cytotoxic, antitumor and immunomodulatory effects of ethanolic extracts of Justicia spicigera leaves (JSE).Materials and methods
The in vitro and in vivo toxicity of JSE was evaluated with comet assay in peripheral blood mononuclear cells (PBMC) and acute toxicity in mice, according to the Lorke procedure, respectively. The apoptotic effect of JSE on human cancer cells and human noncancerous cells was evaluated using flow cytometry with annexin-Alexa 488/propidium iodide. Also, different doses of JSE were injected intraperitoneally daily into athymic mice bearing tumors of HeLa cells during 18 days. The growth and weight of tumors were measured. The in vitro immunomodulatory effects of JSE were evaluated estimating the effects of JSE on the phagocytosis of the yeast Saccharomyces cerevisiae, NO production and H2O2 release in macrophages, as well as the proliferation of splenocytes and NK activity.Results
The comet assay showed that only JSE tested at 200 and 1000 μg/ml induced a significantly DNA damage in PBMC, compared to untreated cells, whereas the LD50 was >5000 mg/kg by intraperitoneal route (i.p.) and by oral route. JSE showed pro-apoptotic (Annexin/PI) effects by 35% against HeLa cells, but lack toxic effects against human normal cells.JSE administrated at 10, 50 and 100 mg/kg i.p. inhibited the tumor growth by 28%, 41% and 53%, respectively, in mice bearing HeLa tumor. JSE stimulated, in a concentration dependent manner, the phagocytosis of Saccharomyces cerevisiae yeasts, the NO production and H2O2 release by human differentiated macrophages. In addition, JSE stimulated the proliferation of murine splenocytes and induced the NK cell activity.Conclusion
Justicia spicigera shows low toxic effects in vitro and in vivo, exerts apoptotic effects on HeLa cells, has antitumor effects in mice bearing HeLa tumor and induces immunomodulatory activities in vitro. 相似文献2.
Angel Josabad Alonso-Castro Elizabeth Ortiz-Sánchez Alejandro García-Regalado Graciela Ruiz José Martín Núñez-Martínez Ignacio González-Sánchez Valeria Quintanar-Jurado Elizabeth Morales-Sánchez Fabiola Dominguez Gabriela López-Toledo Marco A. Cerbón Alejandro García-Carrancá 《Journal of ethnopharmacology》2013
Ethnopharmacological relevance
Justicia spicigera is used for the empirical treatment of cervical cancer in Mexico. Recently, we showed that Justicia spicigera extracts exerted cytotoxic and antitumoral effects and the major component of this extract was kaempferitrin (KM).Materials and methods
The cytotoxic and apoptotic effect of KM on human cancer cells and human nontumorigenic cells were evaluated using MTT and TUNEL assays, and Annexin V/Propidium iodide detection by flow cytometry. The effect of KM on cell cycle was analyzed by flow cytometry with propidium iodide. The apoptotic and cell cycle effects were also evaluated by western blot analysis. Also, different doses of KM were injected intraperitoneally daily into athymic mice bearing tumors of HeLa cells during 32 days. The growth and weight of tumors were measured.Results
KM induces high cytotoxic effects in vitro and in vivo against HeLa cells. The general mechanisms by which KM induces cytotoxic effects include: cell cycle arrest in G1 phase and apoptosis via intrinsic pathway in a caspase dependent pathway. Also, KM exerts chemopreventive and antitumor effects.Conclusion
KM exerts cytotoxic and antitumor effects against HeLa cells. 相似文献3.
R Ortiz-Andrade A Cabañas-Wuan VE Arana-Argáez AJ Alonso-Castro R Zapata-Bustos LA Salazar-Olivo F Domínguez M Chávez C Carranza-Álvarez A García-Carrancá 《Journal of ethnopharmacology》2012,143(2):455-462
Ethnopharmacological importance
Justicia spicigera is a plant species used for the Teenak (Huesteca Potosina) and Mayan (Yucatan peninsula) indigenous for the empirical treatment of diabetes, infections and as stimulant.Aim of the study
To evaluate the cytotoxicity, antioxidant and antidiabetic properties of J. spicigera.Materials and methods
The effects of ethanolic extracts of J. spicigera (JSE) on the glucose uptake in insulin-sensitive and insulin-resistant murine 3T3-F442A and human subcutaneous adipocytes was evaluated. The antioxidant activities of the extract of JSE was determined by ABTS and DPPH methods. Additionally, it was evaluated the antidiabetic properties of JSE on T2DM model.Results
JSE stimulated 2-NBDG uptake by insulin-sensitive and insulin-resistant human and murine adipocytes in a concentration-dependent manner with higher potency than rosiglitazone 1 mM. JSE showed antioxidant effects in vitro and induced glucose lowering effects in normoglycemic and STZ-induced diabetic rats.Conclusion
The antidiabetic effects of administration of J. spicigera are related to the stimulation of glucose uptake in both insulin-sensitive and insulin-resistant murine and human adipocytes and this evidence justify its empirical use in Traditional Medicine. In addition, J. spicigera exerts glucose lowering effects in normoglycemic and STZ-induced diabetic rats. 相似文献4.
Ethnopharmacological relevance
Many studies have been performed to assess the potential utility of natural products as immunomodulatory agents to enhance host responses to disease/infection/etc. or to ameliorate immune based pathologies (i.e., inflammation, autoimmune associated diseases, etc.). In this particular study, the immunomodulatory potential of gall aqueous extract from Limoniastrum guyonianum Boiss. (Zita) was assessed in vitro.Materials and methods
The effect of G extract on splenocytes proliferation and NK activity were assessed by MTT test. The induction of NO production and the phagocytic activity of macrophages were evaluated in vitro. Activation of the cellular anti-oxidant activity in splenocytes was determined by measuring the fluorescence of the DCF product.Results
The studies first demonstrated that the extract could enhance lysosomal enzyme activity and nitrite oxide production in murine peritoneal macrophages, suggesting a potential role in activation of these cells. In studies to assess potential effects on humoral immunity, the results indicated that the extract could significantly promote LPS-stimulated splenocyte proliferation implying a potential activation of B-cells and enhanced humoral immune responses in hosts given this natural product. In studies to assess any effects of extract on cellular immunity, the results showed that the extract significantly enhanced the killing activity of isolated NK cells but had negligible effects on mitogen-induced proliferation of splenic T-cells. Considerable effects were also observed on the cellular anti-oxidant activity.Conclusion
We conclude from these studies that aqueous extract from L. guyonianum gall exhibited an immunomodulator effect which could be ascribed, in part, to its cytoprotective effect via its anti-oxidant capacity. Furthermore, these results suggest that L. guyonianum gall extract contains potent components such as flavonoids which should be potentially used to modulate immune cell functions in physiological and pathological conditions. 相似文献5.
Alonso-Castro AJ Juárez-Vázquez Mdel C Domínguez F González-Sánchez I Estrada-Castillón E López-Toledo G Chávez M Cerbón MA García-Carranca A 《Journal of ethnopharmacology》2012,142(3):857-864
Ethnopharmacological relevance
Phoradendron serotinum is commonly used in Mexican traditional medicine for the empirical treatment of cancer. However, there are no studies regarding the antitumoral or immunomodulatory activities of Phoradendron serotinum.Materials and methods
The in vivo toxicity of ethanolic extracts of Phoradendron serotinum (PSE) was evaluated in mice according to the Lorke procedure. The in vitro immunomodulatory effects of PSE were evaluated estimating the effects of PSE on the pinocytosis, NO production and lysosomal enzyme activity in murine macrophages RAW 264.7. The effects of PSE on the proliferation of murine splenocytes and NK cell activity were also assayed. The cytotoxic effects on TC-1 (lung murine cancer cells) were evaluated using the MTT assay, whereas the apoptotic effect of PSE on TC-1 cells was evaluated using TUNEL assay. Also, different doses of PSE were injected intraperitoneally daily into C57BL/6 mice bearing tumors of TC-1 cells during 25 days. The growth and weight of tumors was measured. In addition, the levels of IL-2, IL-6, IL-12, IL-23 and IFN-γ in murine serum and supernatants of K562 cell—murine splenocyte cocultures were measured.Results
PSE stimulated the proliferation, pinocytosis and lysosomal enzyme activity in murine macrophages with a similar potency than lypopolisaccharides 1 μg/ml. In addition, PSE stimulated the proliferation of murine splenocytes and induced the NK cell activity. PSE showed cytotoxic (IC50 = 1.9 μg/ml) and apoptotic effects against TC-1 cells. The LD50 was 125 mg/kg by intraperitoneal route (i.p.) and 375 mg/kg by oral route. PSE administrated at 1, 5 and 10 mg/kg i.p. inhibited the tumor growth by 18%, 40% and 69%, respectively, in mice bearing TC-1 tumor. PSE increased the in vitro and in vivo release of IL-2, IL-6 and IFN-γ but lacked effect on IL-12 and IL-23 release.Conclusions
Phoradendron serotinum shows moderate toxic effects in vivo, exerts cytotoxic and apoptotic effects on TC-1 cells. Phoradendron serotinum also has antitumor effects in mice bearing TC-1 tumor and induces immunomodulatory activities in vivo. The results suggest that antitumoral effects of PSE are related with the production of immunity-related cytokines. 相似文献6.
Maria del Rosario Jacobo-Salcedo Maria del Carmen Juárez-Vázquez Luis Ángel González-Espíndola Sandra Patricia Maciel-Torres Alejandro García-Carrancá Angel Josabad Alonso-Castro 《Journal of ethnopharmacology》2013
Ethnopharmacological relevance
Cathartes aura is a bird used in the Mexican traditional medicine for the empirical treatment of cancer, injuries, infections and burns.Materials and methods
The in vitro immunomodulatory effects of Cathartes aura extract (CAE) were evaluated estimating its effects on proliferation of human peripheral blood mononuclear cells and murine splenocytes. The effects of CAE (1–200 μg/ml) on NO production, pinocytosis and lysosomal enzyme activity were assayed in murine macrophages RAW 264.7. The cytotoxic effects of CAE (1–500 μg/ml) on tumorigenic and non tumorigenic cells were evaluated using the MTT assay.Results
In the absence of LPS, CAE induced the proliferation of murine splenocytes (119%), enhanced the pinocytosis (113%) and lysosomal enzyme activity (141%) in murine macrophages with a similar potency than lypopolisaccharides 1 μg/ml. In addition, CAE exerted cytotoxic effects mainly on human cervical cancer cells (IC50=117 μg/ml) but lacked toxic effects on non tumorigenic cells (IC50>500 μg/ml).Conclusion
Cathartes aura exerts immunostimulatory and cytotoxic activities 相似文献7.
Giany O. De Melo David do C. Malvar Frederico A. Vanderlinde Fabio F. Rocha Priscila Andrade Pires Elson A. Costa Lécia G. de Matos Carlos R. Kaiser Sônia S. Costa 《Journal of ethnopharmacology》2009
Aim of the study
To identify the compounds responsible for the antinociceptive and anti-inflammatory effects previously described for Sedum dendroideum, through bioassay-guided fractionation procedures.Materials and methods
Antinociceptive activity was evaluated through mouse acetic acid-induced writhing model. The anti-inflammatory activity was assessed through croton oil-induced mouse ear oedema and carrageenan-induced peritonitis.Results
The Sedum dendroideum juice afforded seven known flavonoids identified with basis on NMR data. The oral administration of the major kaempferol glycosides kaempferitrin [1] (17.29 μmol/kg), kaempferol 3-O-β-glucopyranoside-7-O-α-rhamnopyranoside [2] (16.82 μmol/kg), kaempferol 3-O-neohesperidoside-7-O-α-rhamnopyranoside [3] (13.50 μmol/kg) or α-rhamnoisorobin [5] (23.13 μmol/kg) inhibited by 47.3%, 25.7%, 60.2% and 58.0%, respectively, the acetic acid-induced nociception (indomethacin: 27.95 μmol/kg, p.o.; 68.9%). Flavonoids 1, 2, 3 or 5, at the same doses, reduced by 39.5%, 46.5%, 35.6% and 33.3%, respectively, the croton oil-induced oedema (dexamethasone: 5.09 μmol/kg, s.c.; 83.7%) and impaired leukocyte migration by 42.9%, 46.3%, 50.4% and 49.6%, respectively (dexamethasone: 5.09 μmol/kg, s.c.; 66.1%).Conclusions
Our findings show that the major kaempferol glycosides may account for the renowned medicinal use of Sedum dendroideum against pain and inflammatory troubles. 相似文献8.
Aim of the study
The objectives of this study were to evaluate the in vivo antitumor potential of the triterpenoid fraction from the rhizomes of Astilbe chinensis (Saxifragaceae) (Saxifragaceae) (ATF) and to elucidate its immunological mechanisms by determining its effects on the growth of mouse transplanted tumors and the immune response in naïve and tumor-bearing mice.Materials and methods
The mice inoculated with mouse tumor cell lines were treated per os with ATF at the doses of 20, 40, 60 mg/kg for 10 days. The effects of ATF on the growth of transplantable tumor, splenocyte proliferation, the activity of natural killer (NK) cells, and production of interleukin-2 (IL-2) from splenocytes in tumor-bearing mice were measured. Meanwhile, the effects of ATF on 2,4-dinitrofluorobenzene (DNFB)-induced delayed type hypersensitivity (DTH) reaction and the sheep red blood cell (SRBC)-induced antibody response in naïve mice were also studied.Results
ATF could not only significantly inhibit the growth of mice transplantable tumor, but also remarkably increase splenocytes proliferation, NK cells activity, and the level of IL-2 secreted by splenocytes in tumor-bearing mice, promote the DTH reaction and enhance anti-SRBC antibody level in naïve mice, which indicated that the ATF could improve both specific and non-specific cellular and humoral immune response.Conclusions
The antitumor activity of ATF might be achieved by improving immune response, and ATF could act as antitumor agent with immunomodulatory activity. 相似文献9.
Aim of the study
Nigella sativa, also known as blackseed, has long been used in traditional medicine for treating various conditions related to the respiratory and gastrointestinal systems as well as different types of cancers. In this study, the potential immunomodulatory effects of Nigella sativa are investigated in light of splenocyte proliferation, macrophage function, and NK anti-tumor activity using BLAB/c and C57/BL6 primary cells.Materials and methods
Splenocyte proliferation was assessed by [3H]-thymidine incorporation. Griess assay was performed to evaluate NO production by macrophages. ELISA was performed to measure the level of cytokines secreted by splenocytes and macrophages. NK cytotoxic activity against YAC-1 tumor cells was examined by JAM assay.Results
We demonstrate that the aqueous extract of Nigella sativa significantly enhances splenocyte proliferation in a dose-responsive manner. In addition, the aqueous extract of Nigella sativa favors the secretion of Th2, versus Th1, cytokines by splenocytes. The secretion of IL-6, TNFα, and NO; key pro-inflammatory mediators, by primary macrophages is significantly suppressed by the aqueous extract of Nigella sativa, indicating that Nigella sativa exerts anti-inflammatory effects in vitro. Finally, experimental evidence indicates that the aqueous extract of Nigella sativa significantly enhances NK cytotoxic activity against YAC-1 tumor cells, suggesting that the documented anti-tumor effects of Nigella sativa may be, at least in part, attributed to its ability to serve as a stimulant of NK anti-tumor activity.Conclusions
Our data present Nigella sativa as a traditionally used herb with potent immunomodulatory, anti-inflammatory, and anti-tumor effects. We anticipate that Nigella sativa ingredients may be employed as effective therapeutic agents in the regulation of diverse immune reactions implicated in various conditions and diseases such as cancer. 相似文献10.
Aim of the study
The objectives of this study were to investigate the protective effect of the triterpenoid fractions from the rhizomes of Astilbe chinensis (Saxifragaceae) (ATF) on cyclophosphamide (CTX)-induced toxicity in tumor-bearing mice.Materials and methods
The mice inoculated with mouse sarcoma S180 cells were treated with saline or CTX alone, or co-treated with CTX and ATF. The blood, femur bone, and serum samples were collected for determination of the haematological and biochemical parameters and splenocytes were for assay of proliferation, the activity of natural killer (NK) cells, and production of interleukin-2 (IL-2).Results
ATF significantly increased the peripheral white blood cell (WBC) count and bone marrow cellularity (BMC) in CTX-treated S180-bearing mice. Increase of aspartate transanimase (AST), alanine transaminase (ALT), urea nitrogen (BUN), and creatinine in the serum of CTX-treated mice was significantly reversed by ATF. The lowered levels of splenocytes proliferation, NK cells activity, and IL-2 production from splenocytes in S180-bearing mice after CTX treatment were also increased by ATF administration.Conclusions
ATF provides significant protection against CTX-induced hematotoxicity, hepatotoxicity, nephrotoxicity, and immunotoxicity, and might be helpful in abrogation of CTX-induced toxicity during the chemotherapy. 相似文献11.
Aim of the study
Traditional Chinese medicine herbs (TCMHs) are used in medicines as well as in daily dietary supplements in Asia. In this study, we employed pNF-κB-Luc or pIFN-γ-Luc and BALB/c mice peritoneal macrophages or splenocytes to investigate both the immune and inflammatory effects of six selected plant species.Materials and Methods
Specifically, we used ethyl acetate fractions of Astragalus membranaceus (Fisch.) Bunge var. mongholicus (Bunge) Hsiao (Fabaceae) (AM), Andrographis paniculata (Burm. f.) Nees (Acanthaceae) (AP), Angelica sinensis (Oliv.) Diels (Apiaceae) (AS), Eucommia ulmodes Oliv. (Eucommiaceae) leaves (EU leaves), Isatis indigotica Fort. (Brassicaceae) (II) and Morus alba L. (Moraceae) (MA).Results
We found that ethyl acetate fractions of AP, AS and MA significantly decreased NF-κB luciferase activity and also the secretion of NO and PGE2 in LPS/IFN-γ stimulated mouse peritoneal macrophages (p < 0.05). In contrast, they did not affect IFN-γ luciferase activity or IFN-γ production in concanavalin A (Con A)-activated mouse splenocytes. Our results indicated that the anti-inflammatory properties of these plant extracts might be resulted from the inhibition of pro-inflammatory mediators (e.g., NO and PGE2), at least in part via suppression of a signaling pathway such as NF-κB.Conclusions
Collectively, we have found that three potent bioactive TCMH species exerted significant NF-κB inhibitory activity and acted in a cell type dependent fashion. 相似文献12.
13.
Hoang-Yen T. Nguyen Bach-Hue T. Vo Lac-Thuy H. Nguyen Jose Bernad Mohamad Alaeddine Agnes Coste Karine Reybier Bernard Pipy Françoise Nepveu 《Journal of ethnopharmacology》2013
Ethnopharmacological relevance
Crinum latifolium L. (CL) leaf extracts have been traditionally used in Vietnam and are now used all over the world for the treatment of prostate cancer. However, the precise cellular mechanisms of the action of CL extracts remain unclear.Aim of the study
To examine the effects of CL samples on the anti-tumour activity of peritoneal murine macrophages.Materials and methods
The properties of three extracts (aqueous, flavonoid, alkaloid), one fraction (alkaloid), and one pure compound (6-hydroxycrinamidine) obtained from CL, were studied (i) for redox capacities (DPPH and bleaching beta-carotene assays), (ii) on murine peritoneal macrophages (MTT assay) and on lymphoma EL4-luc2 cells (luciferine assay) for cytotoxicity, (iii) on macrophage polarization (production of ROS and gene expression by PCR), and (iv) on the tumoricidal functions of murine peritoneal macrophages (lymphoma cytotoxicity by co-culture with syngeneic macrophages).Results
The total flavonoid extract with a high antioxidant activity (IC50=107.36 mg/L, DPPH assay) showed an inhibitory action on cancer cells. Alkaloid extracts inhibited the proliferation of lymphoma cells either by directly acting on tumour cells or by activating of the tumoricidal functions of syngeneic macrophages. The aqueous extract induced mRNA expression of tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin 6 (IL-6) indicating differentiation of macrophages into pro-inflammatory M1 polarized macrophages. The total flavonoid, alkaloid extracts and an alkaloid fraction induced the expression of the formyl peptide receptor (FPR) on the surface of the polarized macrophages that could lead to the activation of macrophages towards the M1 phenotype. Aqueous and flavonoid extracts enhanced NADPH quinine oxido-reductase 1 (NQO1) mRNA expression in polarized macrophages which could play an important role in cancer chemoprevention. All the samples studied were non-toxic to normal living cells and the pure alkaloid tested, 6-hydroxycrinamidine, was not active in any of the models investigated.Conclusions
Our results indicate that CL extracts and alkaloid fraction (but not pure 6-hydroxycrinamidine) inhibit the proliferation of lymphoma cells in multiple pathways. Our results are in accordance with traditional usage and encourage further studies and in vivo assays. 相似文献14.
Hai-Shun Xu Yuan-Wen Wu Shi-Fang Xu Hong-Xiang Sun Feng-Yang Chen Li Yao 《Journal of ethnopharmacology》2009,125(2):310-317
Aim of the study
The roots of Actinidia eriantha Benth (Actinidiaceae) have been used for cancers in the Chinese folk medicine. The present study aimed at evaluating the antitumor potentials of the polysaccharides from the roots of Actinidia eriantha and elucidating their immunological mechanisms by determining the effects on the growth of tumor transplanted in mice and the immune response in tumor-bearing mice.Materials and methods
The total polysaccharide AEP and fours purified polysaccharides AEPA, AEPB, AEPC and AEPD were isolated and purified from the roots of Actinidia eriantha by hot water extraction, ethanol precipitation, dialysis and gel filtration. Their effects on the growth of mouse transplantable tumor, splenocyte proliferation, the activity of natural killer (NK) cells and cytotoxic T lymphocytes (CTL), production of cytokines from splenocytes, and serum antigen-specific antibody levels in tumor-bearing mice were measured.Results
AEP and four purified polysaccharides could not only significantly inhibit the growth of mouse transplantable tumor, but also remarkably promote splenocytes proliferation, NK cell and CTL activity, IL-2 and IFN-γ production from splenocytes, and serum antigen-specific antibody levels in tumor-bearing mice.Conclusions
The antitumor activity of AEP and four purified polysaccharides might be achieved by improving immune response, and the composition of the monosaccharides, uronic acid contents and molecular weight could affect their antitumor and immunomodulatory activity. 相似文献15.
Jong Rhan Kim Jinhwan Choi Jiyoung Kim Heejeung Kim Heerim Kang Eun Hye Kim Jeong-Hwa Chang Yeong-Eun Kim Young Jin Choi Ki Won Lee Hyong Joo Lee 《Journal of ethnopharmacology》2014
Ethnopharmacological relevance
Ginseng and ginsenosides are frequently used in the treatment of chronic inflammatory diseases. Recently, 20-O-β-d-glucopyranosyl-20(S)-protopanaxadiol (GPD), the main metabolite of ginsenosides, was reported to have both anti-allergic and anti-pruritic effects. The immunomodulatory effects of GPD-fortified ginseng extract (GFGE) on atopic dermatitis (AD)-like symptoms in mice were investigated. This study was designed to investigate the preventive effect of GFGE on AD-like symptoms.Materials and methods
The effects of orally administered GFGE on Dermatophagoides farinae body extract (DFE)-induced AD-like symptoms in NC/Nga mice were assessed by analyzing dermatitis score, ear thickness, scratching time, skin histological changes, and serum level of macrophage-derived chemokine (MDC). In addition, splenocytes were isolated from the mice and stimulated with anti-CD3 and anti-CD28 monoclonal antibodies to produce cytokines.Results
Oral administration of GFGE significantly attenuated DFE-induced increases in dermatitis score, ear thickness, scratching time, and severity of skin lesions in NC/Nga mice. GFGE treatment also reduced level of MDC in serum, infiltration of eosinophils and mast cells in skin, and production of cytokines in splenocytes.Conclusions
These results suggest that GFGE might ameliorate DFE-induced AD-like symptoms and be an alternative therapeutic agent for the prevention of AD. 相似文献16.
Vijayalakshmi D Dhandapani R Jayaveni S Jithendra PS Rose C Mandal AB 《Journal of ethnopharmacology》2012,141(1):542-546
Aim of the study
The anti-inflammatory activity of Aloe vera was investigated through MMP inhibition studies. The effect of Aloe vera on MMP-9 inhibition was tested on peripheral blood mononuclear cells (PBMC).Materials and methods
Peripheral blood mononuclear cells (PBMC) were isolated from the heparinised venous blood by Ficoll Diatrizoate gradient centrifugation. The cell count and viability was determined using dye exclusion technique. Cytotoxicity was evaluated by MTT assay. Activation of MMP-9 was visualized by gelatin zymography. Inhibition of MMP-9 in the presence of aqueous extract of Aloe vera was detected by gelatin zymography and confirmed by RT-PCR.Results
Peripheral blood mononuclear cells (PBMC) showed significant inhibition in the activity of MMP-9, indicating the in vitro inhibitory effect of Aloe vera on MMP-9. Zymographic analysis and RT-PCR showed that it caused a significant reduction in the production of MMP-9 in a concentration dependent manner.Conclusion
The inhibition of MMP-9 production in the cells was detected by gelatin zymography and was confirmed by RT-PCR. 相似文献17.
18.
Angel Josabad Alonso-Castro Rocio Zapata-Bustos José Romo-Yañez Paul Camarillo-Ledesma Maricela Gómez-Sánchez Luis A. Salazar-Olivo 《Journal of ethnopharmacology》2010
Aim of the study
Tecoma stans (L.) Juss. ex Kunth (Bignoniaceae) and Teucrium cubense Jacq (Lamiaceae) are plants extensively used for the empirical treatment of diabetes mellitus, but their antidiabetic mechanisms remain to be clarified. In this study, the effect of aqueous extracts of Tecoma stans (TSE) and Teucrium cubense (TCE) on the glucose uptake in adipose cells was evaluated.Materials and methods
Non-toxic concentrations of TSE and TCE were assayed on the adipogenesis and 2-NBDglucose uptake in insulin-sensitive and insulin-resistant murine 3T3-F442A and human subcutaneous adipocytes.Results
Both extracts stimulated 2-NBDG uptake by insulin-sensitive and insulin-resistant adipocytes in a concentration-dependent manner. In insulin-sensitive cells, TSE 70 μg/ml stimulated 2-NBDG uptake by 193% (murine) and by 115% (human), whereas the same concentration of TCE induced the 2-NBDG uptake by 112% (murine) and 54% (human). In insulin-resistant adipocytes, TSE induced the 2-NBDG uptake by 94% (murine) and 70% (human), compared with the incorporation shown by insulin-sensitive adipocytes stimulated by the hormone, whereas TCE induced the incorporation of 2-NBDG by 69% (murine) and 31% (human). On the other hand, TSE and TCE exerted only minimal or null proadipogenic effects on murine and human preadipocytes.Conclusion
Tecoma stans and Teucrium cubense exert their antidiabetic effects stimulating glucose uptake in both insulin-sensitive and insulin-resistant murine and human adipocytes without significant proadipogenic or antiadipogenic side effects. 相似文献19.
Kim JJ Jiang J Shim DW Kwon SC Kim TJ Ye SK Kim MK Shin YK Koppula S Kang TB Choi DK Lee KH 《Journal of ethnopharmacology》2012,140(2):213-221
Ethnopharmacological evidence
Agrimonia pilosa Ledeb (Rosaceae, AP) has long been used as a traditional medicine in Korea and other Asian countries to treat various diseases.Aim of the study
In the present study, the anti-inflammatory and anti-allergic effects of AP extract in in vitro cell lines and in vivo mouse model of inflammation and the molecular mechanisms involved were reported.Materials and methods
Using Raw 264.7 murine macrophages the effects of methanol extract of AP in lipopolysaccharide (LPS)-induced production of inflammatory mediators were measured. Further IgE-DNP-induced interleukin (IL)-4 production and degranulation in RBL-2H3 rat basophilic cell lines was also estimated. To investigate the anti-asthmatic effect of AP in vivo, airway inflammation in ovalbumin (OVA)-induced mouse model was used.Results
AP attenuated the production of inflammatory mediators such as NO, PGE2 and pro-inflammatory cytokines in LPS-induced Raw 264.7 cells. Further, AP inhibited IL-4 production and degranulation in IgE-DNP-induced RBL-2H3 cells. Furthermore, AP attenuated the infiltration of immune cells into lung, cytokines production in broncho-alveolar lavage fluid (BALF) and airway-hyperresponsiveness (AHR) on OVA-induced mouse model of inflammation.Conclusion
Our results showed that AP attenuated the activation of macrophages, basophils, and inhibited the OVA-induced airway inflammation. The molecular mechanisms leading to AP's potent anti-inflammatory and anti-allergic effects might be through regulation of TRIF-dependent and Syk-PLCγ/AKT signaling pathways, suggesting that AP may provide a valuable therapeutic strategy in treating various inflammatory diseases including asthma. 相似文献20.
Fábio Cruz Moleiro Márcio Aparecido Andreo Raquel de Cássia dos Santos Thiago de Mello Moraes Clenilson Martins Rodrigues Camila Bernardes de Andrade Carli Flávia Cristine Mascia Lopes Cláudia Helena Pellizzon Iracilda Zeppone Carlos Tais Maria Bauab Wagner Vilegas Clélia Akiko Hiruma-Lima 《Journal of ethnopharmacology》2009