Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor α mRNA, glucocorticoid receptor β mRNA and P300/CBP protein in lupus nephritis mice

Objective  

To investigate the therapeutic effects and mechanisms of using artemisinin (Art) combined with glucocorticoid (GC) to treat lupus nephritis (LN) mice. (mice. to treat lupus nephritis     

Association between estrogen receptor β gene Rsa1 polymorphism and depressive disorder in peri-menopausal and menopausal women

Worldwide,theprevalenceofdepressionin womenissignificantlygreaterthaninmen. Availabledatasuggestthatestrogenisstrongly implicatedintheregulationofmoodandbehavior, aswellasinthepathobiologyofmooddisorders[1]. Someresearchers[2,3]administeredtransdermal estradiol0.05mg/dor0.1mg/dtoperi- menopausalwomenwithdepression.After3 weeksofestradioltreatment,mostwomen receivingestradiolexperiencedremissionof depression,comparedwithlesswomenreceiving placebo.ThemeanMont-gomery-Asberg DepressionRatingScal…     

Regulation of estrogen receptors α and β in human breast carcinoma by exogenous leptin in nude mouse xenograft model

Background It is essential to clarify the interactions of hormones during the progression of human breast cancer. This study examined the effects of exogenous human leptin on estrogen receptor （ER） α and β in human breast tumor tissue in a nude mouse xenograft model. Methods We created nude mice xenografts of MCF-7 human breast cancer cells, and randomly divided them into an experimental group and a control group. The mice in experimental group were injected subcutaneously around tumors with human leptin, while the control group were injected with the same dose of normal saline. A real-time RT-PCR assay was developed to quantify the mRNA of ERα, β in the tumor tissues. Western blotting analyses were used to assess the relative quantities of the ERα , β proteins. Results Leptin-treated xenografted nude mice were successfully established. The amount of ERa mRNA was significantly higher in the leptin group than in the control group （P 〈0.01）, while the amount of ERβ mRNA was significantly lower in the leptin group than in the control group （P 〈0.01）. Western blotting analyses revealed that the ERa protein level was significantly higher in the leptin group than in the control group （P 〈0.01）, while the ERβ protein level was significantly lower in the leptin group than in the control group （P 〈0.01）. Conclusions Nude mouse xenograft model can be safely and serviceably treated with human leptin by subcutaneous injections around tumor. ERα, β were both targets of leptin in breast cancer. Leptin can up-regulate the expression of ERa and down-regulate the expression of the ERβ in human breast tumor.     

Progress of surgical treatment of intrahepatic lithiasis in China.

ＰＲＯＧＲＥＳＳＯＦＳＵＲＧＩＣＡＬＴＲＥＡＴＭＥＮＴＯＦＩＮＴＲＡＨＥＰＡＴＩＣＬＩＴＨＩＡＳＩＳＩＮＣＨＩＮＡＨｕａｎｇＺｈｉｑｉａｎｇ黄志强ＤｅｐａｒｔｍｅｎｔｏｆＳｕｒｇｅｒｙ，ｔｈｅＧｅｎｅｒａｌＨｏｓｐｉｔａｌｏｆＣｈｉｎｅｓｅＰＬＡ，Ｂ...     

Keratosis pilaris in pregnancy: an unrecognized dematosis of pregnancy?

Keratosis pilaris (KP) is a common hyperkeratotic condition that most commonly presents as skin-colored follicular papules surrounded by erythema. These lesions often appear grouped together on the lateral aspect of the arms, thighs, and buttocks. Although the etiology of KP is uncertain, it is thought to be the result of the formation of an orthokeratotic plug, which blocks and dilates the orifice and upper portion of the follicular infundibulum (Figure 1). Histologically, mild perivascular mononuclear cell infiltrates are usually present in the adjacent dermis. Studies have indicated a role for hormonal influences in the development of KP. The five cases presented in this study demonstrate keratosis pilaris as a condition in which the onset or severity of the dermatosis may be linked to the hormonal changes of pregnancy.     

Comparison of cleavage activity in vitro between two ribozymes targeted against different sites of PDGF receptor β subunit

Objective: To compare the cleavage activity of ribozymes directed against 2 sites of PDGF receptorP subunit cDNA gene in vitro. Methods: The 608 bp fragment of PDGF receptor β subunit cDNA was clonedinto T-vector under the control of T7 promoter, named pPDGFR-β. Two ribozymes were designed to cleavethe CUU sequence at codon 45 and codon 252 of PDGF receptor β subunit mRNA respectively. These 2 ham-merhead ribozyme genes were cloned into vector PI. 5 between 5' -cis ribozyme and 3' -cis ribozyme to gener-ate the plasmids of pRZ1 and pRZ2. The pPDGFR-β, pRZl and pRZ2 were linearized and then transcribedwith T7 promoter in vitro. Results: The RZ1 showed high cleavage activity in vitro, but the RZ2 showed nocleavage activity under the same condition. Conclusion: The cleavage site selection is an important factor in-fluencing the cleavage activities of ribozymes.     

Prognostic factors of fulminant hepatitis in pregnancy

Fhulamvein saenvte hreep satatitteis a (nFdH c)om rpefleicrsat etod lmivaenri fdeissteaatisoenss ·thaItt will endanger patients’lives and health largely·The incidence of fulminant hepatitis in pregnancy (FHP) is 66 times of patients not in pregnancy,1 which is more dangerous and is one of the major causes of maternal and perinatal death in China· In order to predict the prognoses of FHP and decrease maternal and perinatal death rate, we summarized clinical information of 25 cases of FH…     

Effects of estrogen on growth of breast cancer cells and expression of p185 and EGF receptor

Ｅｆｆｅｃｔｓｏｆｅｓｔｒｏｇｅｎｏｎｇｒｏｗｔｈｏｆｂｒｅａｓｔｃａｎｃｅｒｃｅｌｌｓａｎｄｅｘｐｒｅｓｓｉｏｎｏｆｐ１８５ａｎｄＥＧＦｒｅｃｅｐｔｏｒＨａｏＸｉｎｂａｏ；ＺｈａｎｇＹｉｎｇｈｕａ；ＱｉｕＦｕｈａｉ；ＹｉｎＹｉｎｇ；ＷａｎｇＬｉｌｉ...     

Effects of Placental Isoferritin on the Mouse Embryo Development in vitro

To investigate the effect of placental isoferritin （PLF） on mouse embryo development in vitro, mice 2-cell embryos were co-cultured with human first trimester decidual cells at different concentrations of PLF in vitro. The following changes of the above system were observed under an invert microscope and the number of embryos were recorded and the embryos were classified. The results showed there was no significant difference in the percentage of embryos development to 4-cell 8-cell and morula （P〉0.05）. PLF at the doses of 10 and 100 U/mL significantly enhanced more embryos development to the blastocyst and hatching blastocyst （P〈0.05）. PLF at the dose of 1000 U/mL depressed more embryos development from 2-cell to hatching blastocyst, meanwhile such phenomena as cell degeneration and irregular cleavage were observed in part of embryos, but there was no significant difference in statistics （P〉0.05）. It was concluded that PLF at the concentration of 10-- 100 U/mL had no significant effects on the early development of mice embryos, however, PLF could promote the growth, differentiation, and hatching of preimplantion blastocysts.     

Three cases of hyperlipidemic pancreatitis in late pregnancy

Hyperlipidemic pancreatitis occurring in late pregnancy is a rare event with high morbidity and mortality. We report on 3 cases of acute hyperlipidemic pancreatitis during late pregnancy.     

Isosorbide mononitrate upregulates platelet-derived growth factor receptor β and promotes angiogenesis in zebrafish

目的 通过研究5-单硝酸异山梨酯对斑马鱼血管发育的影响,探讨硝酸酯类药物促进血管新生的作用机制,方法 以血管内皮具有绿色荧光蛋白标记的转基因斑马鱼胚胎为研究对象,将以100、300、500、1 000 μg/ml 5-单硝酸异山梨酯处理的胚胎作为实验组,以0.5％二甲基亚砜(DMSO)处理的胚胎作为对照组,从受精卵形成后0.5～1 h开始药物处理,24～26 h后观察药物对转基因斑马鱼血管发育的影响.使用荧光显微镜观察新生血管状态并记录血管长度.分别从药物处理后0、6、12和24 h的斑马鱼胚胎中提取总RNA,采用实时定量RT-PCR方法检测血小板源性生长因子受体β(PDGFRβ)的表达变化.结果 5-单硝酸异山梨酯可促进斑马鱼血管发育(P＜0.01),且在100、300、500 μg/ml 3个浓度梯度范围内其促进作用与药物浓度成正比,实验组较对照组PDGFRβ表达量升高,在药物作用12和24 h时差异有统计学意义(P＜0.05,P＜0.01).结论 5-单硝酸异山梨酯可上调PDGFRβ的表达,促进斑马鱼血管发育.     

An observation of the anastomoses of intrahepatic veins in normal human adults.

ＡｎｏｂｓｅｒｖａｔｉｏｎｏｆｔｈｅａｎａｓｔｏｍｏｓｅｓｏｆｉｎｔｒａｈｅｐａｔｉｃｖｅｉｎｓｉｎｎｏｒｍａｌｈｕｍａｎａｄｕｌｔｓＨｅＳｈｅｎｇ何生，ＧｕｏＺｉｊｉａｎ郭子健ａｎｄＷｕＹａｎｔａｏ吴言涛Ｈｅｐａｔｏ－Ｐａｎｃｒｅａｔｏ－Ｂｉｌｉａ...     

Determination of the concentration of estrogen,progestin and androgen cytosol receptors in human uterine tissues

The specific bindings of estrogen,progestin and androgen were determined inthe cytosol fraction of myomatous,adenomyotic and postmenopausal uterine tissues andof the normal endometrium and myometrium as well.It was found that theconcentrations of estrogen,progestin and androgen cytosol receptors were significantlyhigher in myomatous tissue than in normal myometrium;there was also an obvious differ-ence of the concentration of the sex steroid receptors between normal endometrium andadenomyotic tissue;and the uterine tissues of postmenopausal women still retained highlevels of these sex steroid receptors.In addition,the regulation of sex steroids in thepathogenesis of myoma and adenomyosis is discussed.     

Significance of changes in serum superoxide dismutase level in hypertensive syndrome of pregnancy

Authors measured the concentration of human serum superoxide dismutase 1 (hSOD-1) containing copperand zinc ions by radioimmunoassay healthy nonpregnant women, 15 normal pregnant women, 15 patients with mildto moderatc hypertension (MMHSP) and 15 with severe hypertensive syndrome of pregnancy (SHSP ). The mean     

A study on the expressions and the correlation of TGF-β1 and α-SMA in healing process of bile duct trauma

Objective: To explore the formation mechanism of benign biliary stricture. Methods: A model of trauma of common bile duct was established in 28 dogs and then repaired. The anasomosis tissues were taken on the 1 st week,3rd week and the 3rd month, 6th month respectively after operation and examined by using light microscopy and electromicroseopy. Macrophage, TGF-β1, and α-SMA were studied immunohistochemically. Results: The mucosal epithelium of common bile duct restored poorly, chronic inflammation lasted for a long time, fibroblasts proliferated actively,extracellular matrix overdeposited; and myofibroblasts functioned actively and existed during the whole healing process.test showed a high expression of macrophage, TGF-β1, and α-SMA during healing process lasting a long duration. Macrophages were found in the lamina propria under mucosa, TGF-β1, in the granulation tissue, fibroblasts and endothelial cells of blood vesssels, while α-SMA in the myofiroblasts and smooth muscle tissue. Conclusion: The healing of bile duet is in the mode of overhealing. Myofibroblast is the main cause for contracture of scar andstricture of bile duet. The high expression of macrophage, TGF-β1, and α-SMA is closely related to active proliferation of fibroblasts, extracelluar matrix overdeposition and sear contracture of bile duct.