全氟三丁胺在复杂性视网膜脱离术中的应用

目的：评估国产全氟三丁胺在复杂性视网膜脱离术中的应用价值。方法：在玻璃体切除术中使用全氟三丁胺撑开漏斗状视网膜脱离,逐出视网膜下液,碾平和固定视网膜,平复翻转的巨大视网膜裂孔瓣。结果：术中所有视网膜巨大裂孔患者均复位,增殖性玻璃体视网膜病变（ＰＶＲ）－Ｄ２－Ｄ３者６０％全复位,４０％部位复位,共８眼获得视力增进,结论：在复杂性视网膜脱离玻璃切除术中应用全氟三丁胺,能提高手术成功率,减少术中和术后的     

硅油在复杂性视网膜脱离手术中的应用及临床观察

应用玻璃体切割联合硅油填充术治疗复杂性视网膜脱离９例（９只眼），其中合并ＰＶＲＣ３～ＰＶＲＤ２７只眼，巨大视网膜裂孔合并ＰＶＲＤ３１只眼，巨大视网膜裂孔１只眼。除１只为巨大视网膜裂孔眼以前未行手术外，余均为３～５次手术失败眼。随诊２～２３个月，平均１２．６个月。视网膜完全复位３只眼，视力均在０．０２以上。３只眼术后取出硅油，其中１只眼复发视网膜脱离。硅油填充对常规视网膜复位术无法治愈的视网膜脱离有一定治疗作用；但并发症较多，故需严格掌握适应证。术后４～６个月视网膜复位，色素上皮与神经上皮粘连牢固，可取出硅油。     

玻璃体视网膜手术中全氟丙烷的应用

目的　观察全氟丙烷在视网膜复位及玻璃体手术中的眼内填充效果。方法　对38例视网膜脱离患者，在视网膜复位手术和玻璃体手术中于玻璃体腔内注入全氟丙烷。结果　随访3—12个月，33例患者视网膜解剖复位，视力不同程度提高。结论　应用全氟丙烷能够简化视网膜复位手术，促进裂孔愈合；用于玻璃体手术具有加强展平视网膜的作用。     

玻璃体切除术治疗复发性复杂性视网膜脱离

目的;评价玻璃体切除术治疗复发性复杂性视网膜脱离的效果。方法：对５８例（５７眼）复发性复杂性视网膜脱离患者行玻璃体切除联合眼内激光或冷冻,惰性气体或硅油眼内充填治疗,术后密切观察随访３～２４个月（平均９．６个月）。结果：玻璃体切除术后全部视网膜解剖复位,最后随访时的视网膜复位率９１．２％（５２／５７眼）。视网膜复位眼最后视力均不同程度的提高,达０．０２～０．７。并发症主要有医源必裂孔,暂时性高眼压     

显微镜直视下最小量手术方法治疗裂孔源性视网膜脱离56例临床观察

目的 探讨显微镜直视下最小量外路视网膜脱离手术的可行性及治疗效果.方法 对56例（56眼）非复杂性孔源性视网膜脱离患者在显微镜直视下行前房放液、视网膜裂孔定位、冷凝、巩膜外加压、顶压硅胶加压块检查裂孔是否封闭,观察患者术中术后并发症、术后眼压、术后视力及视网膜复位情况,术后随访3～ 12个月.结果 51例一次手术视网膜完全复位（91.1％）,术中出现前房少量出血1例,术后出现裂孔形成鱼嘴状3例,行二次单纯加压块调位固定后均复位,2例因发生增殖性玻璃体视网膜病变造成裂孔开放做了玻璃体切割手术后复位.术后出现暂时性高眼压2例.术后视力提高48眼（85.7％）.结论 显微镜直视下行最小量裂孔源性视网膜脱离手术具有视野清晰、损伤小、裂孔冷凝定位可靠、手术操作简单、容易掌握、术后效果好的优点,值得临床推广应用.     

玻璃体切割联合术后短期全氟化碳液体填充治疗巨大视网膜裂孔

目的:研究术后短期全氟化碳液体填充治疗巨大视网膜裂孔引起的视网膜脱离患者的疗效和安全性。方法:对因巨大视网膜裂孔引起视网膜脱离患者而行玻璃体切割,并用全氟化碳液体作为术后短期填充治疗的连续病例进行回顾性分析。5～14d(珔x=7.5d)后取出全氟化碳,并以气体或硅油填充。对部分伴有增生性玻璃体视网膜病变的病例行巩膜扣带术。切除影响手术中视野或半脱位的晶状体。评价视网膜复位的成功率、视力及术后并发症。结果:对61例患者的62只眼随访8～69个月(珔x=24.5个月)。术后所有视网膜均复位,14只眼(22.6%)视网膜再次脱离,其中13只眼给…     

复杂性黄斑裂孔视网膜脱离显微手术的护理干预

复杂性黄斑裂孔视网膜脱离患病后致盲率高。我院2000年以来采用玻璃体手术联合12％～16％的非膨胀浓度全氟丙烷气体治疗复杂性黄斑裂孔视网膜脱离40例，取得良好的效果，现报告如下。     

视网膜脱离复位术后再手术分析

目的探讨视网膜脱离手术失败或术后复发影响因素及其再手术治疗效果。方法以同期一次手术成功50例患眼作对照，回顾分析再次行视网膜脱离复位手术54例患眼视网膜脱离相关病情，并研究采用不同手术方式再次行视网膜复位的治疗效果。结果54例再次行视网膜脱离复位手术眼，发现原裂孔未封闭或再次裂开19只眼（36．1％），新裂孔或遗漏裂孔30只眼（55．6％）。大部分患眼伴有不同程度玻璃体视网膜增殖病变（PVR）。再次手术11只眼行玻璃体腔注气术，22只眼行巩膜扣带术，21只眼行玻璃体切除术，其成功率分别为72．7％、90．9％和85．7％。结论裂孔封闭不全是视网膜脱离复发或未能复位直接原因，PVR是形成新裂孔、影响裂孔闭合及视网膜复位的主要因素。视网膜脱离再次手术成功关键仍是可靠地封闭裂孔，应针对具体病情采取不同手术方式。     

玻璃体手术中眼内激光光凝联合全氟化碳气体治疗复杂性视网膜脱离

目的　评价在玻璃体手术中眼内激光光凝联合全氟化碳气体治疗复杂性视网膜脱离的效果及与冷凝治疗效果的临床比较。方法　回顾性分析 2 0 0 1年 6月至 2 0 0 3年 8月间在玻璃体手术中眼内激光光凝封闭视网膜裂孔联合全氟化碳气体治疗复杂性视网膜脱离 2 5眼和冷凝封闭视网膜裂孔治疗视网膜脱离 2 9眼共 5 4例 (5 4眼 )的临床资料。结果　光凝组 2 2眼裂孔封闭 ,视网膜复位 ,手术复位率 88 0 0 %。冷凝组 2 4眼裂孔封闭 ,视网膜复位 ,手术复位率 82 76 % (χ2 =0 2 92 3,P =0 0 5 )。术后视力提高光凝组 2 1眼 (84 0 0 % ) ,冷凝组 2 2眼 (75 86 % ) (χ2 =0 5 4 81,P >0 0 5 )。结论　经眼内激光光凝能安全、有效地封闭视网膜裂孔 ,能达到与冷凝封闭视网膜裂孔同样的效果。全氟化碳气体充填有利于视网膜脱离复位     

复杂性黄斑裂孔视网膜脱离围手术期护理干预的临床意义

为了探讨护理干预对玻璃体切割联合非膨胀浓度的全氟丙烷(C3Fs)气体治疗复杂性黄斑裂孔视网膜脱离的临床意义。选取玻璃体切割联合12％～16％的C3Fa气体治疗复杂性黄斑裂孔视网膜脱离30例30眼，实施护理干预，对照组30例30眼行常规护理，观察其疗效及并发症。结果2组治愈28例，术后并发白内障，干预组2例，非干预组9例。术后高眼压：干预组5例，非干预组14例。可见护理干预能提高复杂性黄斑裂孔视网膜脱离的治疗效果，降低并发症的发生。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.