脊髓内催产素参与电针镇痛

<正> 催产素(Oxytocin,OT)是在下丘脑室旁核和视上核内合成的一种垂体肽类激素,除有促进排乳和使子宫收缩的作用外,它的另一些作用正日益引起人们的关注。一些研究表明,中枢神经系统内的OT在痛调制和针刺镇痛过程中发挥着一定作用,但脊髓水平的OT对电针镇痛是否发挥作用,     

中缝大核内催产素在电针镇痛中的作用

目的 :探讨中缝大核 (NRM )内催产素 (OT)在电针镇痛中的作用 ,及其和内源性 5 HT在电针镇痛中的关系。方法 :以钾离子透入法引起大鼠甩尾反应的电流强度为痛行为反应指标 ,测定动物痛阈 ,观察NRM内注射催产素和噻庚啶对电针镇痛的影响。结果 :向大鼠NRM内微量注入催产素后 ,能明显增加电针镇痛的效应 ;而向NRM内注入噻庚啶后 ,可阻断催产素增加电针镇痛的效应。结论 :NRM内的催产素在电针镇痛的复杂过程中发挥着一定作用 ,且其效应可能是通过内源性 5 HT系统中介的。     

催产素与八肽胆囊收素对电针镇痛的影响

<正> 近年来国内外文献报道,中枢神经系统的催产素(OT)在痛觉调制过程中发挥着一定的作用,我们最近的工作也表明,脑和脊髓内的OT不仅可以提高大鼠的痛阈,而且能明显增强电针镇痛效应。为进     

外周乙酰胆碱对电针镇痛作用的影响

<正> 为了探讨外周乙酰胆碱(ACh)与针刺穴位引起感觉信息一级传入的关系,本文用不能通过血脑屏障的胆碱酯酶抑制剂(新斯的明)和乙酰胆碱合成阻断剂(密胆碱)及乙酰胆碱外周给药,以升高或降低外周乙酰胆碱     

中脑导水管周围灰质内催产素参与电针镇痛

以钾离子透入法引起大鼠甩尾反应的电流强度（ｍＡ）为痛反应指标，通过向中脑导水管周围灰质（ＰＡＧ）内注射催产素（ＯＴ），抗催产素血清（ＡＯＴＳ）和纳洛酮（Ｎｘ），观察了ＰＡＧ内ＯＴ对动物电针镇痛的影响。结果表明，ＰＡＧ内生理水平的ＯＴ参与电针镇痛的过程，并且其作用不依赖于内源性阿片肽系统。     

四种作用于肾上腺素受体药物对电针镇痛的影响

<正> 肾上腺素受体具有广泛的临床意义,近年发展迅速。脑内去甲肾上腺素神经递质,主要通过α受体对抗针刺镇痛。α受体在脑内有α_1和α_2两种,它们对电针镇痛的影响如何?本文研究特异作用于α_2肾上     

催产素与加压素在针刺镇痛中的作用（之二）加压素对电针镇痛的影响

已有报道，精氯酸加压素(AVP)可以提高动物的基础痛阈。本实验以钾离子透入—甩尾反应为痛指标，理察了大鼠脑室注射AVP及其抗血清对电针足三里的镇痛效应的影响，并采用放射免疫分析法测了电针时脑内AVP含量的变化，探讨AVP在电针镇痛中所起的作用。     

电针镇痛对大鼠脑组织多胺物质含量的影响

我们以前工作发现,鼠脑组织碳酸酐酶和ATP酶的活性,氧耗量和c-AMP含量与针刺镇痛相关.最近研究证明,多胺物质通过对酶的修饰而影响细胞生命过程。并象二信使物一样调节CNS的功能。本研究的目的在于了解针刺镇痛与大鼠脑多胺物质是否有关.动物实验的结果提示,内源性多胺物质与针刺镇痛的效果有关,腐胺和多胺物质的含量都明显高於对照组(P<0.01)。我们的发现意味着在针刺镇痛中内源性多胺物质所反映出来的实际水平,呈现一种对CNS的综合性调节效应。我们认为:这些论据与针刺镇痛的分子机理有关。     

Therapeutic effects of electroacupuncture (EA) on acute experimental arthritis in rats

The dynamic changes of the pain thresholds (Heat-Withdrawal Latency, HWL and Stimulation-Vocalization Threshold, Voc) in the adjuvant-induced arthritic rats and the effects of EA on the pain thresholds, skin temperature and the circumference of the diseased ankle were observed. Both pain thresholds were obviously decreased in affected limbs in EA and control groups. But in the normal limbs, HWL decreased and Voc, no changed. In EA group, the ankle circumference and skin temperature were markedly lower than that in control group. The increase of the body weight was much more obvious than that in control group. Our experiment revealed that EA could not only increase the pain threshold, but also improve the pathological changes in the inflammation of the ankle, the mechanisms of the changes of the pain thresholds and the effects of EA in the arthritic rats were discussed.     

大鼠中缝背核在电针镇痛中变化的超微结构研究

<正> 中缝背核(NRD)在疼痛调节和针刺镇痛过程中起重要作用,并参与内分泌、情感、体温、饮食活动和睡眠与觉醒周期等多种生理功能的调节。电解损毀NRD可明显减弱电针的镇痛效果     

Effect of melatonin and electroacupuncture (EA) on NK cell activity,interleukin-2 production and POMC-derived peptides in traumatic rats

The present study was to evaluate the effect of melatonin (MT) and EA on the cytotoxic activity of natural killer (NK) cells, the dynamic changes of the induction of interleukin-2(IL-2) and the content of POMC-derived peptides, beta-endorphins (betaE) and ACTH in spleen lymphocytes and in plasma of traumatic rats. The results showed that intraperitioneal (i.p.) injection of MT was able to recover the lower levels of NK cell activity and the induction of IL-2 production; MT could also decrease the higher betaE and ACTH levels induced by trauma in spleen lymphocytes and plasma. EA needling of Zusanli (St.36) and Lanwei (Extra.37) points obviously improved the immunosuppression produced by trauma and antagonized the elevation of betaE and ACTH contents induced by trauma stress in spleen lymphocytes and plasma. MT + EA could further modulate the depressed immune function, and there was a significant difference compared with MT (i.p.) or EA alone. MT + EA group further decreased the betaE and ACTH contents in immune cells and plasma. Yet, the mechanisms of the attenuation of MT and EA on immunosuppression induced by trauma need further study.     

电针对逆向刺激所致神经性炎症的效应

<正> 很早就有人发现,外周神经逆向刺激可引起外周神经血管的扩张和血浆渗出,用染色剂注入血管与血浆蛋白结合后随血浆渗出可在被刺激的神经支配区域皮肤上     

外周C纤维传入在电针镇痛中作用的观察

<正> 针刺镇痛的研究中,外周神经中哪一类纤维参与针刺镇痛效应一直是个争论的问题。候宗濂等认为C纤维传入是主要的,张香桐等提出外周神经中Ⅱ、Ⅲ类纤维是重要的,吕国蔚等则看到主要是Aβr类     

Effect of morphine and electro-acupuncture (EA) on apoptosis of thymocytes

The influence of morphine and EA on the apoptosis of thymocytes were studied to investigate the posibility of its involvement in the mechanism of morphine-induced immunosuppression and the regulatory effect of EA on it. 1h after injecting 50 mg/kg morphine subcutaneously into 3-wk old Balb/c mice continually twice a day for 5 days, thymus was collected and the apoptotic cell was detected by a method of terminal deoxynucleotidyl transferase-meditaed dUTP nick end-labeling(TUNEL). The results showed that morphine significantly enhanced the percentage of TUNEL positive cells inside thymus with an appearing of apoptotic DNA ladder after 24 h incubation. Treating mice with EA of "Zusanli(St.36)" and "Lanwei(Ext.33)" for 1h after morphine administration decreased the percentage of TUNEL positive cells. EA also showed an regulatory effect on the increased the expression of CPP32 and decreased the expression of Bcl-2 by morphine. The significant enhancement of hypothalamic CRF and plasma ACTH level by morphine and the antagonize effect of EA on it suggested a possible role of Hypothalamus-pituitary-adrenal (HPA) axis played in the apoptosis of thymocytes by morphine and the regulatory effect of EA.