共查询到20条相似文献,搜索用时 109 毫秒
1.
目的:研究急性乙型肝炎和慢性乙型肝炎PBMC培养上清液中IL-12水平与血清ALT及TBIL之间的关系。方法:应用FicollHypaque密度梯度离心法从肝素化血中分离外周血PBMC,用ELISA方法检测PBMC培养上清液IL-12和血清ALT及TBIL水平。结果:急性乙型肝炎和慢性乙型肝炎PBMC培养上清液中IL-12水平与血清ALT及TBIL水平呈正相关。结论:急性乙型肝炎和慢性乙型肝炎PBMC培养上清液中IL-12水平与肝功炎症活动密切相关。 相似文献
2.
目的探讨保肝降酶治疗对慢性乙型肝炎(慢乙肝)患者血清IL-17和抗核抗体(ANA)水平的影响。方法对30例慢乙肝患者(慢肝组)给予复方甘草酸苷制剂治疗3周,随机抽取乙肝病毒携带者30例为对照组,检测血清IL-17和ANA表达。结果慢肝组患者使用复方甘草酸苷制剂治疗前后IL-17和ANA表达均无明显差异[(182.546±17.731)pg/ml vs.(177.201±16.247)pg/ml和0.867±0.446vs.0.645±0.285](P>0.05);但ANA表达均明显高于对照组的0.555±0.281(P<0.01和P<0.05)。结论仅使用保肝降酶治疗,不能有效降低血清IL-17及ANA水平。 相似文献
3.
HBV感染者血清白介素(12,2,10)的水平及临床意义 总被引:2,自引:0,他引:2
目的检测HBV不同感染状态时血清IL-12、IL-2和IL-10水平,并探讨其临床意义。方法选择HBV不同感染状态,包括无症状携带者、无症状携带者重叠急性自限性肝炎、HBeAg阳性慢性乙型肝炎、HBeAg阴性慢性乙型肝炎和急性乙型肝炎患者等作为研究对象,以健康献血员作对照,采用双抗体夹心ELISA法检测血清IL-12、IL-2和IL-10水平并进行比较。结果无症状携带者血清IL-10水平明显高于健康对照,部分无症状携带者血清IL-12和IL-2高水平;重叠感染HAV、HEV感染IL-2、IL-10和IL-12水平急性期和恢复期相比无明显差异;慢性乙型肝炎患者发病急性期血清IL-12水平低下,血清IL-2和IL-10水平均较健康对照明显升高;急性乙型肝炎患者血清IL-12和IL-2水平与ALT水平并非完全平行关系,血清IL-10水平与ALT水平呈完全平行关系。结论血清IL-12和IL-2水平可考虑作为无症状携带者抗病毒治疗指征,慢性乙型肝炎活动与血清IL-12水平低下有关,血清IL-2水平升高与肝炎活动有关,其升高主要参与促进HBeAg/HBeAb血清转换。肝炎活动与血清IL-10水平无明显相关性。 相似文献
4.
目的:探讨干扰素-γ(IFN-γ)、肿瘤坏死因子(TNF)-α及白细胞介素(IL)-6在脑瘫中的作用。方法:采用酶联免疫吸附法检测28例脑瘫患儿及15例正常儿童外周血单核细胞(PBMC)中IFN-γ、TNF-α及IL-6的水平。结果:脑瘫患儿PBMC中IFN-γ、TNF-α、IL-6分别为(46.17±14.29)pg/ml、(28.65±11.44)pg/ml、(67.13±21.10)pg/ml,对照组分别为(37.31±16.64)pg/ml、(18.43±10.97)pg/ml、(46.44±23.39)pg/ml,差异均有统计学意义(P<005)。结论:脑瘫患儿IFN-γ、TNF-α及IL-6水平的升高,可能与脑瘫的发生有关,即脑瘫的发生与免疫系统及其功能异常有密切关系。 相似文献
5.
目的探讨白介素-5(IL-5)在分泌性中耳炎发生发展过程中的作用机制。方法选择2010年3月-2012年9月我院耳鼻咽喉头颈外科确诊的分泌性中耳炎患者24例28耳作为试验组,其中以病程<3个月的患者为急性组,病程≥3个月的患者为慢性组。另从我院体检科体检的健康人员中选取22例健康人员作为对照组。采用双抗体夹心酶联免疫吸附试验(ELISA)检测所有人员血清中IL-5的表达水平。结果试验组中耳积液中IL-5浓度为(10.437±1.393)pg/ml高于血清中的(2.754±0.868)pg/ml,差异有统计学意义(P<0.01)。试验组血清中IL-5浓度(2.754±0.868)pg/ml低于对照组的(2.876±1.074)pg/ml,差异无统计学意义(P>0.05)。慢性组中耳积液中IL-5浓度为(11.374±1.457)pg/ml高于急性组的(9.550±0.776)pg/ml,差异有统计学意义(P<0.05)。结论 IL-5作为重要的变态反应性细胞因子,存在于分泌性中耳炎患者中耳积液中,中耳局部产生变态反应性炎症,IL-5可能在分泌性中耳炎的慢性病程中发挥重要作用。 相似文献
6.
目的探讨TNF-α和IL-6在重症急性胰腺炎(SAP)血清中的变化及临床意义。方法应用ELISA法检测23例重症急性胰腺炎和30例轻型急性胰腺炎(MAP)及20例对照组血清TNF-α、IL-6的水平变化。结果入院时SAP患者TNF-α水平(59.1±15.5)pg/ml显著高于MAP组(38.9±9.72)pg/ml和对照组(14.2±4.5)pg/ml,MAP患者血清TNF-α水平也较对照组显著升高,但IL-6水平SAP(45.5±11.7)pg/ml和MAP(43.4±13.3)pg/ml较对照组(39.3±11.0)pg/ml无明显升高;SAP血清IL-6在入院的第7天升高最明显(188.5±51.7)pg/ml,分别高于MAP组(115.8±27.8)pg/ml和对照组,MAP亦明显高于对照组;在入院第14天SAP血清TNF-α(36.4±7.6)pg/ml,IL-6(112.6±25.2)pg/ml仍然高于MAP(15.4±3.7)pg/ml、(44.7±13.2)pg/ml和对照组,而MAP与对照组无明显差别。结论检测血清TNF-α和IL-6的水平变化,对重症急性胰腺炎的早期诊断、病情判断和预后评估具有重要的应用价值。 相似文献
7.
目的探讨白细胞介17(IL-17)在慢性阻塞性肺疾病(COPD)发病机制中的作用。方法抽取100例COPD急性加重期(A1组)、100例稳定期患者(A2组)及100例健康人(C组)静脉血标本,采用双抗体ELISA法测定血清IL-17水平。并作肺功能检查,计算第1秒用力呼气容积比(FEV1%)。结果 A1组血清IL-17明显高于A2组[(73.1±58.4)pg/mlvs.(34.8±21.6)pg/ml](P<0.01),A2组血清IL-17高于C组[(16.4±7.6)pg/ml](P<0.01)。A1组血清IL-17与FEV1%呈负相关(P<0.05)。结论 IL-17可能参与COPD的发病。 相似文献
8.
白细胞介素6与慢性乙型肝炎患者肝细胞损伤和纤维化的关系 总被引:1,自引:0,他引:1
目的探讨血清白细胞介素6(IL-6)与慢性肝病的关系.方法采用ELISA法和放免法对76例慢性乙型肝炎(CHB)、肝炎肝硬化(LC)、慢性重型肝炎(CSH)患者血清IL-6和Ⅲ型前胶原蛋白(PCⅢ)及Ⅳ型胶原蛋白(ⅣC)测定,肝活检42例进行肝组织病理学分析.结果①CHB轻、中、重度、LC、CSH患者血清IL-6水平分别为(29.93±8.08)pg/ml、(50.36±4.51)pg/ml、(78.33±9.11)pg/ml、(71.59±8.23)pg/ml、(96.13±5.47)pg/ml,依次升高(P<0.01或P<0.05);明显高于对照组(7.10±1.80)pg/ml.②血清IL-6水平与PCⅢ及ⅣC呈正相关(P<0.01),与肝组织炎症损伤及纤维化程度亦呈正相关.结论IL-6水平与慢性肝病严重程度关系密切. 相似文献
9.
武忠长 《国际医药卫生导报》2016,(10):1457-1459
目的 分析肺结核患者治疗前后外周血中IL-10、IL-22、IL-23表达水平变化及其临床意义.方法 收集本院2014年10月至2015年5月收治的活动性肺结核患者49例,选择健康体检者30例为对照组.ELISA法检测肺结核患者治疗前、治疗6个月后以及对照组外周血血清中IL-10、IL-22、IL-23表达水平,并分析其相关性.结果 ELISA结果显示,肺结核患者治疗前外周血血清IL-10表达水平为(24.15±13.21) pg/ml,明显高于对照组[(14.91±8.23) pg/ml],P<0.05,经抗结核常规治疗6个月后,血清IL-10表达水平为(17.11 ± 7.59) pg/ml,明显低于治疗前(P<0.05),但仍高于对照组.肺结核患者治疗前外周血血清IL-22、IL-23表达水平分别为(20.56±7.41) pg/ml、(21.12 ± 2.68) pg/ml,明显低于对照组[(53.78±8.22) pg/ml、(36.42±2.16) pg/ml,P<0.05],经抗结核常规治疗6个月后,血清IL-22、IL-23表达水平分别为(38.95±6.35) pg/ml、(29.89±3.74) pg/ml,明显高于治疗前(P<0.05),但仍明显低于对照组(P< 0.05).Pearson相关分析结果表明,血清IL-22、IL-23呈正相关性.结论 肺结核患者外周血中IL-10、IL-22、IL-23在治疗前后表达水平发生变化,可能与机体结核免疫有关,有望作为评价肺结核疗效的参考指标. 相似文献
10.
11.
12.
《Journal of addictive diseases》2013,32(3):73-87
Depression and anxiety frequently coexist in patients with substance use disorders. This clinically-oriented article examiens the relationship between these conditions and emphasizes data showing that substances of abuse can cause signs and symptoms of both depression and anxiety. These substance-related syndromes appear to have a different course and prognosis than uncomplicated, independent anxiety and major depressive disorders, and clinicians should consider the role of alcohol and other drugs in all patients presenting with these complaints. The authors will also outline an approach for diagnosing and managing patients with the combination of a substance use and depressive or anxiety disorder. 相似文献
13.
The synthesis of gaultherin (1) and its analogs was carried out to provide 11 glycosides under phase-transfer catalytic conditions. The activities of all synthesized compounds were evaluated by nitric oxide production inhibitory assay in vitro. Methyl 2-O-(4-O-β-d-galactopyranosyl)-β-d-glucopyranosylbenzoate (5f) showed significantly anti-nociceptive and anti-inflammatory effects by the evaluation in vivo. Structure–activity relationships within these compounds were discussed. 相似文献
14.
Nestorov I 《Toxicology letters》2001,120(1-3):411-420
Two important methodological issues within the framework of the variability and uncertainty analysis of toxicokinetic and pharmacokinetic systems are discussed: (i) modelling and simulation of the existing physiologic variability in a population; and (ii) modelling and simulation of variability and uncertainty when there is insufficient or not well defined (e.g. small sample, semiquantitative, qualitative and vague) information available. Physiologically based pharmacokinetic models are especially suited for separating and characterising the physiologic variability from the overall variability and uncertainty in the system. Monte Carlo sampling should draw from multivariate distributions, which reflect all levels of existing dependencies in the intact organism. The population characteristics should be taken into account. A fuzzy simulation approach is proposed to model variability and uncertainty when there is semiquantitative, qualitative and vague information about the model parameters and their statistical distributions cannot be defined reliably. 相似文献
15.
16.
17.
The effects of the d and l isomers of amphetamine on self-stimulation responding were tested following acute and chronic administration. Tolerance and post-drug depression of responding occurred in tests with both isomers, indicating no role for p-hydroxynorephedrine (PHN) which is one of the metabolites of d-amphetamine. In the second experiment, d-amphetamine, methylphenidate and cocaine all produced quantitatively and qualitatively similar effects on self-stimulation responding following acute administration. Following chronic administration of d-amphetamine, animals showed tolerance to all three drugs, indicating cross-tolerance among them. These data are consistent with an hypothesis that tolerance and post-drug depression following chronic amphetamine treatment are the result of decreases in postsynaptic receptor sensitivity, which would lead to a decreased effectiveness of all three drugs, regardless of their pre-synaptic mechanisms. 相似文献
18.
Rationale Two pharmacotherapies are approved for treating alcohol craving (acamprosate and naltrexone), but both have shown mixed findings
in animals and humans.
Objectives The present experiments utilized a “reinforcer blocking” approach (i.e., rats were able to consume ethanol during treatment)
to better understand the efficacy of these treatments for ethanol seeking and drinking using ethanol-dependent and nondependent
rats.
Materials and methods In “nondependent” experiments, drugs (acamprosate 50, 100, and 200 mg/kg; naltrexone 0.1, 0.3, and 1.0 mg/kg) were administered
over 3-week periods prior to operant sessions with a low response requirement to gain access to reinforcers for 20 min. For
“dependent” experiments, rats were made dependent in vapor/inhalation chambers.
Results Acamprosate and naltrexone had similar effects on intake in nondependent and dependent rats; neither drug was selective for
ethanol over sucrose drinking. In nondependent animals, naltrexone was more efficacious at more doses than acamprosate, and
acamprosate’s effects were limited to a dose that also had adverse effects on body weight. Both pharmacotherapies showed more
selectivity when examining reinforcer seeking. In nondependent rats, acamprosate and naltrexone had response-attenuating effects
in ethanol, but not sucrose, groups. In dependent animals, acamprosate had selective effects limited to a decrease in sucrose
seeking. Naltrexone, however, selectively decreased ethanol-seeking in nondependent rats.
Conclusions The naltrexone-induced decreases in seeking suggested a change in incentive motivation which was selective for ethanol in
nondependent rats. The “nondependent” paradigm may model early stages of “problem drinking” in humans, and the findings suggest
that naltrexone could be a good intervention for this level of alcohol abuse and relapse prevention. 相似文献
19.
Schierholz JM Yücel N Rump AF Beuth J Pulverer G 《International journal of antimicrobial agents》2002,19(6):81-516
Catheters, urethral and ureteral stents and other urological implants are frequently affected by encrustration and infection due to their permanent contact with urine. Indwelling urinary catheters provide a haven for microorganisms and thus require extensive monitoring. Several surface modification techniques have been proposed to improve the performance of devices including the immobilization of biomolecules, the incorporation of hydrophilic grafts to reduce protein adsorption, the creation of hydrophobic surfaces, the creation of microdomains to regulate cellular and protein adhesion, new polymers and antimicrobial coatings. Physico-chemical explanation to elucidate the mechanism of such encrustation or infection inhibiting materials is still not available. Our series of experiments showed a marked decrease of silver-activity in biological fluids which corresponds with the controversial clinical results obtained with silver coated urinary catheters. Rifampicin/minocycline coated catheters had very low activity against Gram-negative rods, enterococci and Candida spp., the main causing organisms of urinary catheter infection. Surface engineered materials and antimicrobial drug delivery systems will be the next generation of sophisticated urinary catheters and stents, if both efficacy as well as efficiency has been proved clinically. 相似文献
20.
Max Caviezel Werner K. Lutz Urs Minini Christian Schlatter 《Archives of toxicology》1984,55(2):97-103
[6,7-3H] Estrone (E) and [6,7-3H]estradiol-17 (E2) have been synthesized by reduction of 6-dehydroestrone and 6-dehydroestradiol with tritium gas. Tritiated E and E2 were administered by oral gavage to female rats and to male and female hamsters on a dose level of about 300 g/kg (54 mCi/kg). After 8 h, the liver was excised from the rats; liver and kidneys were taken from the hamsters. DNA was purified either directly from an organ homogenate or via chromatin. The radioactivity in the DNA was expressed in the units of the Covalent Binding Index, CBI = (mol chemical bound per mol DNA-P)/(mmol chemical administered per kg b.w.). Rat liver DNA isolated via chromatin exhibited the very low values of 0.08 and 0.09 for E and E2, respectively. The respective figures in hamster liver were 0.08 and 0.11 in females and 0.21 and 0.18 in the males. DNA isolated from the kidney revealed a detectable radioactivity only in the female, with values of 0.03 and 0.05 for E and E2, respectively. The values for male hamster kidney were < 0.01 for both hormones. The minute radioactivity detectable in the DNA samples does not represent covalent binding to DNA, however, as indicated by two sets of control experiments. (A) Analysis by HPLC of the nucleosides prepared by enzyme digest of liver DNA isolated directly or via chromatin did not reveal any consistent peak which could have been attributed to a nucleoside-steroid adduct. (B) All DNA radioactivity could be due to protein contaminations, because the specific activity of chromatin protein was determined to be more than 3,000 times higher than of DNA. The high affinity of the hormone to protein was also demonstrated by in vitro incubations, where it could be shown that the specific activity of DNA and protein was essentially proportional to the concentration of radiolabelled hormone in the organ homogenate, regardless of whether the animal was treated or whether the hormone was added in vitro to the homogenate.Carcinogens acting by covalent DNA binding can be classified according to potency on the basis of the Covalent Binding Index. Values of 103–104 have been found for potent, 102 for moderate, and 1–10 for weak carcinogens. Since estrone is moderately carcinogenic for the kidney of the male hamster, a CBI of about 100 would be expected. The actually measured limit of detection of 0.01 places covalent DNA binding among the highly unlikely mechanisms of action. Similar considerations can be made for the liver where any true covalent DNA binding must be below a level of 0.01. It is concluded that an observable tumor induction by estrone or estradiol is unlikely to be due to DNA binding.Paper presented at the Satellite Symposium of the European Society of Toxicology, Rome, March 29, 1983 相似文献