Analysis of current nuclear cardiology literature in MEDLINE database: A study of gated SPECT imaging using PubMed

BACKGROUND: Easy access to relevant clinical information is necessary for physicians to make the best decisions for patient management. The increasing amount of information available has made it difficult for physicians to retrieve up-to-date information efficiently. We sought to determine the accessibility and accuracy of indexing in the nuclear cardiology literature by conducting sample searches in the MEDLINE database on the topic of gated single photon emission computed tomography (SPECT) imaging. METHODS AND RESULTS: The MEDLINE database was initially searched by use of both a primary and a comprehensive search strategy on PubMed for publications in English from 1994 to 2000. A total of 260 papers were retrieved from the primary search and 306 additional papers from the comprehensive search. Only 204 of the 566 citations from the combined electronic searches were truly relevant to gated SPECT. The resulting specificity index (precision) was 36%. A hand search was conducted in 11 top journals from 1994 to 2000. It yielded 81 additional citations that were missed by the PubMed search. The sensitivity index (recall) was calculated for all 11 journals. The Journal of Nuclear Cardiology had the highest rate of publication but the lowest rate of recall (44%). The clinical nuclear cardiology terminology and classifications were compared with the available Medical Subject Headings (MeSH) and MeSH Trees used for indexing in MEDLINE. CONCLUSIONS: There are 6 nuclear cardiology techniques, including gated SPECT and myocardial perfusion imaging, that are not specifically indexed in the current MEDLINE database. The lack of specific MeSH headings and indexing structure results in low recall and precision for retrieval of nuclear cardiology literature. We recommend 2 additions to the MeSH Tree Structure and 6 new MeSH headings.     

基于NCBI开放接口的学科信息平台PubMed检索服务功能实现

作为全球最大的网络生物医学文献数据库,美国PubMed在生物医学研究领域有着不可替代的作用。生物医学领域的科研人员已经习惯于利用PubMed查找所需要的信息资源。该文简要介绍了NCBI提供的Web Serv-ice接口功能,以及如何利用该接口在将PubMed检索服务内嵌到学科服务平台中,并将PubMed服务与图书馆的文献传递服务进行了尝试性的集成。     

Uterine Fibroid Embolization or Myomectomy: How Much Marketing Is Enough? Comparative Analysis of Public Search Trends in Google and Medical Publications in PubMed

To compare public popularity and volume of scientific publications regarding uterine fibroid embolization (UFE) and myomectomy. Google Trends and PubMed data were queried to assess temporal variations in online public search volumes and number of research publications for UFE and myomectomy. Time series analysis was used to identify meaningful temporal trends and forecast a future trend. Compared with UFE, myomectomy had significantly higher volumes of public online search and research publications, with an increasing trend over time (P < .0001). The forecasting models predicted a continuing increase in both public search volumes and number of research publications for myomectomy and static future trends in these metrics for UFE. This study signals significantly lower public popularity and research efforts for UFE compared with myomectomy for uterine fibroids. More effective marketing strategies and further research support will be needed to fill this gap.     

基于文献计量学的耐辐射异常球菌研究态势分析

目的 分析国内外关于耐辐射异常球菌（DR）的研究现状,明确前沿与热点问题,初步评析其发展前景,为进一步的研究提供信息和方向。 方法 以Web of Science和中国知网数据库为文献来源,检索2009至2018年主题词为“耐辐射异常球菌”、“耐辐射奇球菌”、“Deinococcus radiodurans”的相关文献,根据发文量、发文期刊、作者信息、关键词以及国家等进行文献计量分析,同时通过国内外文献可视化分析的权威工具CiteSpace V.5.5软件对以上信息进行多元、分时、动态地分析,探索它们之间的关系,分析其研究的发展态势、热点及前沿领域等。 结果 共纳入1026篇英文文献和157篇中文文献。英文文献数量近十年呈持平递减趋势;中文文献数量则呈波动变化,其引证文献却呈上升的趋势。英文发文量最多的作者是HUA YJ（华跃进）（48篇),中文发文量最多的作者是陈明（14篇）。国外主要发文期刊是美国的Plos One和Journal of Biological Chemistry,国内的则是《核农学报》;进行这些研究的机构主要是大学和原子能研究中心,发文量前3的国家为美国、中国和法国;研究热点主要围绕DR的抗性基因,具体研究方向较分散。 结论 DR的研究主要集中在其抗性基因,因此其耐辐射的机制今后依然是一个研究热点,且其在医学治疗、农业和环境治理等方面的应用研究具有很大的探索空间。     

耐辐射奇球菌PprI蛋白质在毕赤酵母菌中的高效表达及纯化

目的 利用真核毕赤酵母高效表达原核的耐辐射奇球菌pprI基因,建立高效表达及纯化PprI蛋白质的技术路线。方法 根据毕赤酵母密码子的偏爱性,改造耐辐射奇球菌pprI基因的编码序列,利用PCR技术全合成改造过的pprI基因,并在其N末端添加一个6×His标签。PCR产物经纯化后克隆到毕赤酵母表达载体pHBM-905A中,利用Cop I和Not I双酶切并回收线性化的目的片段后,转化毕赤酵母GS115菌株。将获得的毕赤酵母转化子诱导表达,SDS-PAGE、Western blot和质谱检测培养上清液,用Ni-NTA柱纯化目的蛋白,BCA法测定蛋白浓度。结果 新合成的耐辐射奇球菌pprI基因编码序列正确,毕赤酵母转化菌株的培养上清液经SDS-PAGE和Western blot均可检测到相对分子质量为43000的目的蛋白条带,经质谱检测证实该目的蛋白为耐辐射奇球菌PprI蛋白。选用Ni-NTA柱获得了大量纯化的PprI蛋白,应用浓度为250 mmol/L的咪唑淋洗时,PprI蛋白洗脱率最高。BCA法测得纯化蛋白浓度为0.35 mg/ml。结论 建立了一种新的适于毕赤酵母表达的耐辐射奇球菌pprI基因,成功构建了分泌表达PprI蛋白质的重组毕赤酵母工程菌株,建立了高效表达目的蛋白的技术路线,并获得了高效表达和纯化的PprI蛋白。     

NOMOS-Kommentar zum Strafgesetzbuch (NK/StGB). Herausgegeben von Urs Kindh?user, Ulfried Neumann und Hans-Ullrich Paeffgen.

Ohne Zusammenfassung     

新型细胞凋亡分子探针99Tcm-CP3-peptide的制备及在肺腺癌细胞系体内外的实验研究

目的 制备含天冬氨酸-谷氨酸-缬氨酸-天冬氨酸（DEVD）核心的多肽细胞凋亡分子探针99Tcm-CP3-peptide,研究其在肺腺癌细胞系A549化疗后体内外的生物学分布及SPECT显像。 方法 采用双功能螯合法合成99Tcm-CP3-peptide,分别于标记后0、1、2、4、6 h行高效液相色谱（HPLC）法检测放射性化学纯度。对经紫杉醇化疗的肺腺癌A549细胞进行体外细胞结合实验,对照组未经化疗,测量细胞内外放射性比值（Cin/Cout）,并用流式细胞仪检测细胞凋亡率。经紫杉醇化疗的荷瘤裸鼠尾静脉注射99Tcm-CP3-peptide 3.7 MBq,分别于5、15、30、60、120、240 min测其生物学分布,并行SPECT静态显像,勾画感兴趣区（ROI）,计算肿瘤/对侧正常肌肉组织的比值（T/NT）。取显像后的肿瘤组织进行苏木精-伊红染色,观察肿瘤组织的形态学变化。两组比较采用t检验,相关性研究采用双变量相关分析。 结果 99Tcm-CP3-peptide在0、1、2、4和6 h的放射化学纯度均>97%,合成率为（64.5±5.2）%。体外细胞结合实验结果表明,经紫杉醇化疗后24h,肿瘤细胞Cin/Cout为10.27±2.02,对照组Cin/Cout为1.09±0.03,化疗组为对照组的7.3倍。对同状态的肿瘤细胞行流式细胞检测,化疗组细胞凋亡率为（75.62±2.57）%,对照组细胞凋亡率为（3.42±0.32）%,化疗组和对照组Cin/Cout与流式细胞仪检测的凋亡细胞百分比呈明显正相关（r=0.970,P<0.05）。荷瘤裸鼠体内分布实验结果显示,99Tcm-CP3-peptide在血液中清除速度较快,且主要经肾脏代谢;心脏、脾脏、肺等重要器官放射性摄取值均较低;但在肝脏中摄取稍高且代谢速度较慢;肿瘤组织摄取值在注射后1 h达到峰值[（4.26±1.03）%ID/g]。荷瘤小鼠给药后1 h即可获得清晰图像,勾画ROI显示:化疗组T/NT为3.83±0.11,显著高于对照组的1.57±0.09,两者间差异有统计学意义（t=16.19, P<0.05）。病理结果显示化疗组肿瘤组织见大量核固缩、核碎裂及凋亡细胞;对照组仅见少量核固缩、核碎裂及少量凋亡细胞。 结论 99Tcm-CP3-peptide是一种靶向caspase-3的显像剂,具有较好的生物学分布,可应用于动物模型肿瘤组织凋亡显像,在监测化疗后肿瘤细胞凋亡方面有一定的临床潜在价值。