共查询到9条相似文献,搜索用时 4 毫秒
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目的探讨核因子-κB(nuclear factor-kappa B,NF-κB)在耳蜗的表达情况。方法24只小鼠随机平均分为4组,其中2组分别连续3d和7d于皮下注射卡那霉素(kanamycin,KA),一组鼓室注射脂多糖(lipopolysac-charides LPS),另一组皮下注射等量生理盐水7d作为对照,用多克隆兔抗体NF-κB p65免疫组织化学等染色,观察NF-κB p65在小鼠耳蜗的表达。结果NF-κB p65主要定位在耳蜗的螺旋缘、盖膜、血管纹、螺旋韧带、螺旋神经节和神经纤维。整个耳蜗各回均可观察到免疫反应,而螺旋韧带、盖膜、螺旋突、螺旋神经节和神经纤维均可观察到较强的NF-κB p65免疫反应,Corti器的免疫反应比上述结构要弱一些。Corti器的内、外毛细胞,内、外柱细胞,Deiter细胞和Claudious细胞均可观察到NF-κB p65的免疫反应,这些免疫反应在内沟细胞、Hensen细胞和Claudious细胞显示较弱,而在螺旋神经节较强,内、外毛细胞核未见免疫反应。省去一抗的对照染色未观察到NF-κB p65的阳性反应。用脂多糖和卡那霉素处理的小鼠耳蜗NF-κB p65的免疫反应与注射正常生理盐水的小鼠耳蜗有显著性差异,而注射脂多糖和卡那霉素3d和7d NF-κB p65的免疫反应无差异。结论注射脂多糖和卡那霉素可使NF-κB p65在耳蜗的表达增强。 相似文献
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目的:探讨高脂血症对小鼠耳蜗的影响。方法:20只昆明种小鼠随机平均分为2组,其中1组建立高脂血症模型,另1组作为对照,6周后用多克隆兔抗NF-κB p65免疫组织化学染色,观察NF-κB p65在高脂血症小鼠耳蜗中的表达,并检测小鼠ABR反应阈。结果:在高脂血症小鼠耳蜗中,NF-κB p65主要定位在Corti器、盖膜、血管纹、螺旋韧带、螺旋神经节及其神经纤维,高脂血症小鼠耳蜗NF-κB p65的免疫反应与对照组比较显著增强。高脂血症模型组小鼠ABR反应阈与对照组比较明显增高,差异有统计学意义(P<0.01)。结论:高脂血症可使小鼠耳蜗NF-κB p65表达增强,ABR反应阈增高,表明高脂血症可引起小鼠听力损害。 相似文献
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Michael S. Hildebrand Hans-Henrik M. Dahl Jennifer Hardman Bryony Coleman Robert K. Shepherd Michelle G. de Silva 《Journal of the Association for Research in Otolaryngology》2005,6(4):341-354
The low regenerative capacity of the hair cells of the mammalian inner ear is a major obstacle for functional recovery following
sensorineural hearing loss. A potential treatment is to replace damaged tissue by transplantation of stem cells. To test this
approach, undifferentiated and partially differentiated mouse embryonic stem (ES) cells were delivered into the scala media
of the deafened guinea pig cochlea. Transplanted cells survived in the scala media for a postoperative period of at least
nine weeks, evidenced by histochemical and direct fluorescent detection of enhanced green fluorescent protein (EGFP). Transplanted
cells were discovered near the spiral ligament and stria vascularis in the endolymph fluid of the scala media. In some cases,
cells were observed close to the damaged organ of Corti structure. There was no evidence of significant immunological rejection
of the implanted ES cells despite the absence of immunosuppression. Our surgical approach allowed efficient delivery of ES
cells to the scala media while preserving the delicate structures of the cochlea. This is the first report of the survival
of partially differentiated ES cells in the scala media of the mammalian cochlea, and it provides support for the potential
of cell-based therapies for sensorineural hearing impairment. 相似文献
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目的 探究一氧化氮(nitricoxide,NO)在一过性微栓塞耳蜗缺血性损伤中的作用。方法 20只豚鼠随机分为实验组和对照组各10只,实验组动物通过磁微粒栓塞造成实验性耳蜗缺血模型;以扫描电镜观察缺血耳蜗听纤毛变化;以免疫组织化学方法观察原生型一氧化氮合酶(constitutive nitric oxide synthase,cNOS)在两组耳蜗的表达变化。结果 实验性耳蜗缺血造成内外毛细胞听纤毛散在的粘结、倒伏或缺失,内毛细胞听纤毛病变明显;内皮型一氧化氮合酶(endothelial NOS,eNOS)表达分布于蜗轴及内侧螺旋板内神经纤维、螺旋神经节、内侧螺旋缘、Corti器细胞及血管纹/螺旋韧带区;神经型一氧化氮合酶(neuron NOS,nNOS)主要分布于蜗轴及内侧螺旋板内神经纤维、螺旋神经节细胞,在Corti器细胞、血管纹细胞及内侧螺旋缘上皮细胞有较弱的表达;实验性缺血组豚鼠耳蜗与正常对照组耳蜗原生型一氧化氮合酶的表达无差异。结论 微栓塞耳蜗缺血引起散在听毛细胞损伤;两种原生型NOS在耳蜗固有表达,分布有交叉,功能存在相互代偿,但未发现因微栓塞缺血而引起变化。 相似文献
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DING Da-lian WANG Jian YU Zhi-ping JIANG Hai-yan WANG Ping Richard Salvi 《中华耳科学杂志(英文版)》2008,3(2):76-83
Cells in mammalian cochleae virtually stop proliferation and exit cellular circle before birth. Consequently, hair cells and spiral ganglion neurons destroyed by ototoxic factors cannot be replaced through proliferative regeneration. However, substantial proliferation occurs in organotypic cultures of cochleae from postnatal mice. In the present study, we studied the time course of proliferative growth in cultures of mouse cochlea explants obtained from up to 12 postnatal days. The mitotic nature of this growth was confirmed by bromodeoxyuridine (BrdU) staining and expression of proliferation cell nuclear antigen (PCNA) evaluated with real-time quantitative polymerase chain reaction(RT-PCR). Similar growth time course was found in the cochlear explants of different postnatal ages. The new growth reached its maximum at around 2 days in culture followed by a slow-down, and virtually stopped after 5 days of culture. The possible mechanisms and the significance of this proliferation are discussed. 相似文献
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喉癌患者多药耐药相关蛋白基因的表达及其临床意义 总被引:1,自引:0,他引:1
目的:研究多药耐药相关蛋白基因(MRP mRNA)的表达与喉癌患者临床病理特征之间的关系。方法:应用逆转录-多聚酶链反应(RT-PCR)技术,检测了35例喉癌患者MRP mRNA的表达。结果:喉癌组织中MRP mRNA阳性表达率为45.7%(16/35),其阳性表达强度在晚期肿瘤中明显增高(P〈0.05),且与肿瘤颈淋巴结转移明显相关(P〈0.01),结论:MRP mRNA在喉癌中的表达,不仅提示 相似文献
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Tae Wook Kim Sung Yong Choi Young Hyeh Ko Chung-Hwan Baek Young-Ik Son 《Clinical and experimental otorhinolaryngology》2012,5(4):207-212