EFFECT OF ELECTROACUPUNCTURE ON SYNAPTIC PLASTICITY OF HIPPOCAMPAL NEURONS IN CEREBRAL ISCHEMIA RATS

Objective: To observe the effect of electroacupuncture (EA) on synaptic structure of hippocampal nerve felts and synaptophysin(SYN)expression in rats with cerebral ischemic injury. Methods: Sixty Wistar rats were randomized into sham-operation group, cerebral ischemia (CI) group and EA group, each of which was further divided into 1week (W) and 5W subgroups. Cl injury model was established by occlusion of the bilateral common carotid arteries. "Baihui"(百会 GV 20), "Dazhui" (大椎 GV 14), "Renzhong"(人中 GV26) and "Guanyuan"(关元 CV 4) were punctured and stimulated electrically. The brain tissue sections containing hippocampus region were stained with immu nohistochemical technique and observed under light microscope and transmission electronic microscope. Results: After CI, the ischemic injury as degeneration of the presynapse compositions, decrease of the synaptic numeral density, and low expression of SYN were observed in hippocampal CA1 area. By the 5th week after CI, the neonatal synapses of CI and     

EFFECT OF ELECTROACUPUNCTURE OF DUMAI-ACUPOINTS ON CEREBRAL NO AND BLOOD ENDOTHELIN CONTENTS IN RATS WITH ACUTE CEREBRAL ISCHEMIA

Ｉｎｒｅｃｅｎｔｙｅａｒｓ,ｔｈｅｓｔｕｄｙｏｎａｃｕｐｕｎｃｔｕｒｅｔｒｅａｔｍｅｎｔｏｆａｃｕｔｅｉｓｃｈｅｍｉｃｃｅｒｅｂｒｏｖａｓｃｕｌａｒｄｉｓ ｅａｓｅｓｈａｓａｃｈｉｅｖｅｄｇｒｅａｔｅｒｐｒｏｇｒｅｓｓ[1 ] .Ｉｎｏｒｄｅｒｔｏｓｔｕｄｙｍｅｃｈａｎｉｓｍｓｏｆａｃｕｐｕｎｃｔｕｒｅｔｒｅａｔｍｅｎｔｏｆｉｓｃｈｅｍｉｃｃｅｒｅｂｒｏｖａｓｃｕｌａｒｄｉｓｅａｓｅｓ,ｗｅｏｂｓｅｒｖｅｄｔｈｅｅｆｆｅｃｔｏｆＥＡｏｆＢａｉｈｕｉ(ＧＶ 2 0 )ａｎｄＤａｚｈｕｉ(ＧＶ 1 4)ｏｎｎｉｔｒｉｃｏ…     

脑络通对大鼠局灶性脑缺血脑细胞超微结构的影响

采用大脑中动脉阻断所致大鼠脑缺血模型,观察了具有益气活血功效的脑络通对脑梗塞范围、白细胞计数、脑细胞超微结构的变化。结果发现,脑络通能显著缩小脑梗塞范围,抑制脑缺血后脑实质白细胞的浸润。在电镜下观察缺血边缘区的病理变化,脑络通组细胞器形态基本正常,而模型组则细胞器溶解,细胞变性、坏死。表明脑络通对缺血脑细胞有显著保护作用     

电针对局灶性脑缺血／再灌注大鼠大脑皮层IL—1RImRNA和蛋白表达的调节

目的 观察脑缺血对IL－1RI的表达及电针对其表达的调节。方法 采用SD大鼠大脑中动脉线栓法制备MCAO缺血模型,应用原位杂交和免疫组化的方法观察脑缺血及电针对大鼠大脑皮层白细胞介素－1受体（Ⅰ型）（interleukin-1 receptor type Ⅰ,IL-1RI)mRNA和蛋白表达的影响。结果 在正常及假手术大鼠IL－1RImRNA和蛋白阳性细胞在皮层呈基础水平表达;脑缺血再灌注后2hIL-1RImRNA阳性细胞表达增加,6h达高峰。脑缺血后IL－1RI蛋白在缺血侧皮层表达明显增加,再灌注后12h达高峰。电针可显著降低MCAO大鼠大脑皮层IL－1RImRNA和蛋白的表达。结论 脑缺血后IL－1RImRNA和蛋白表达增加,电针可降低IL－1RImRNA和蛋白的表达,从而减轻缺血后IL－1β导致的缺血性脑损伤。     

针刺对大鼠局灶性脑缺血再灌注损伤后神经元凋亡的影响

目的：观察针刺对大鼠局灶性脑缺血再灌注损伤后海马神经元内细胞周期因子（cyclin D1,CDK4）和神经元凋亡的影响。方法：大脑中动脉线栓法建立脑缺血再灌注损伤模型,应用免疫印迹法和流式细胞计数分别检测细胞周期因子和细胞凋亡。结果：再灌注48小时后海马细胞周期因子（cyclin D1,CDK4）表达升高,凋亡细胞增多,针刺组cyclin D1,CDK4表达和凋亡细胞明显减少（P〈0．01）。结论：针刺对脑缺血再灌注损伤有防护作用,其机制可能与调控细胞周期因子从而抑制凋亡有关。     

EFFECT OF ELECTROACUPUNCTURE ON SERUM IL-1β AND TNF-α CONTENTS IN CEREBRAL ISCHEMIA-REPERFUSION RATS

Purpose: To study the mechanism of early acupuncture treatment in resisting inflammatory injury in cerebral ischemia-reperfusion (IR) rats. Methods: A total of 120 Wistar rats were randomly divided into normal control, sham-operation, model and EA groups, and each of them were further divided into IR-12 hr, IR-24 hr and IR-48 hr subgroups, with 10 cases being in each subgroup. Cerebral ischemia was produced by occlusion of the middle cerebral artery for 0.5 hr. Shuigou (GV 26) and Baihui (GV 20) were punctured and stimulated electrically with an EA therapeutic apparatus. Serum IL-1β and tumor necrosis factor (TNF)-α contents were assayed by using radioimmunoassay.Results: Results showed that serum IL-1β and TNF-c contents of model group were significantly higher than those of normal control and sham-operation groups at IR 12 hr, 24 hr and 48 hr ( P ＜ 0.05), meaning that following IR, the production and release of these two cytokines in the blood increased obviously; comparison between EA and model groups displayed that serum IL-1β and TNF-α levels of EA group were strikingly lower than those of model group (P＜0.01),suggesting that EA stimulation can suppress IR induced increase of production and release of these two cytokines. Conclusion: EA can suppress cerebral IR induced increase of serum IL-1β and TNF-α, which may contribute to the effect of acupuncture in resisting cerebral IR injury in the treatment of stroke.     

STUDY ON THE EFFECT OF ACUPUNCTURE ON CEREBRAL ISCHEMIA IN MICE

Ｍｅｎｔａｌｒｅｔａｒｄａｔｉｏｎ (ＭＲ)ｉｓａｌｓｏｃａｌｌｅｄａｓｏｌｉｇｏｐｈｒｅｎｉａｏｒｍｅｎｔａｌｄｅｆｅｃｔｏｃｃｕｒｒｉｎｇｄｕｒｉｎｇｄｅｖｅｌｏｐｍｅｎｔｓｔａｇｅ .Ｉｔｍａｎｉｆｅｓｔｓｍａｉｎｌｙａｓｄｉｓ ｔｕｒｂａｎｃｅｉｎｐｅｒｃｅｐｔｉｏｎ ,ｍｅｍｏｒｙ ,ｓｐｅｅｃｈａｎｄｆｉｎｅｍｏｖｅｍｅｎｔ ,ｅｔｃ ..Ｔｈｅｔｈｅｒａｐｅｕｔｉｃｅｆｆｅｃｔｏｆ“ＪＩＮＳａｎＺｈｅｎ”ｔｈｅｒａｐｙｍａｉｎｌｙｆｏｒｔｒｅａｔｍｅｎｔｏｆｉｎ ｆａｎｔｉｌｅＭＲｈａｓｂｅｅｎｃ…     

不同穴位针刺对暂时性局部脑缺血大鼠皮层体感诱发电位和脑梗塞体积的影响

观察不同穴位针刺对暂时性局部脑缺血的作用，采用皮层体感诱发电位测定和ＴＴＣ染色法，结果表明，电针能促进皮层体感诱发电位的恢复，可使脑缺血后脑梗塞体积减小，且督脉穴位针刺在促使脑缺血后皮层体感诱发电位恢复上可能具有相对较强的作用。     

STUDY ON PROTECTIVE ACTION OF ELECTROACUPUNCTURE ON INJURY OF NEURONS IN RATS OF FOCAL ISCHEMIA BY MICROSCOPY AND ELECTRON MICROSCOPY

Cerebrovasculardiseaseisoneofthefirstsixseverediseasesindeathcausesinourcoun-try.Amongcerebrovasculardiseases,morbidityofischemicdiseaseisthehighest.Ischemicin-juryofneuronsoncereachestheirreversiblestage,regenerationandrenovationofneuronsaredifficult.Thu…     

EFFECT OF ELECTROACUPUNCTURE ON NOREPINEPHRINE LEVEL AND APOPTOSIS IN CEREBRAL CORTEX TISSUE IN RATS WITH CEREBRAL ISCHEMIA-REPERFUSION

Ischemiccerebrovasculardiseaseisanin creasinglydangerousfactortohuman’shealthandlifequality .Resumingthesurvivalabilityofthepatientswithischemiccerebrovasculardiseasemaximallywillbethefocusattractingtheatten tionofmedicalworkers.Cellularapoptosis,awayofcelldeathdifferingfromtheconventionalnecroticway,isamajordeathmodeforthecellswithischemicimpairment,particularly ,forthoseinthevulnerablecerebralareaundergonetheshort term partialischemia .Inrecentyearsmonoamineshavebeengraduallyknowntoplayanim…     

绞股蓝丹对小鼠脑缺血再灌注损伤的脑保护作用

目的 :探讨绞股蓝丹对脑缺血再灌注损伤脑保护的作用机制。方法 :采用小鼠颈总动脉结扎再灌注模型 ,测定脑匀浆中丙二醛 (MDA)、一氧化氮 (NO)含量、总抗氧化能力 (T_AOC)及过氧化氢酶 (CAT)活性 ,探讨预防性给予绞股蓝丹对脑组织的保护作用机理。结果 :绞股蓝丹能明显提高小鼠脑缺血再灌注损伤脑组织的T_AOC和CAT活性 ,明显抑制损伤脑组织中的MDA升高。结论 :绞股蓝丹可通过抗氧化机制达到脑保护功能。     

EFFECT OF ACUPUNCTURE ON PLASMA cAMP AND cGMP IN RATS WITH ACUTE CEREBRAL INFARCTION

Ａｃｕｔｅｃｅｒｅｂｒａｌｉｎｆａｒｃｔｉｏｎｉｓｏｎｅｏｆｔｈｅｍｏｓｔｃｏｍｍｏｎｌｙｅｎｃｏｕｎｔｅｒｅｄｃｅｒｅｂｒｏｖａｓｃｕｌａｒｄｉｓｅａｓｅｓ,ａｃｃｃｏｕｎｔｉｎｇｆｏｒ75%[1].Ｒｅｓｅｎｔｙｅａｒｓ’ｒｅｓｅａｒｃｈｅｓｉｎｄｉｃａｔｅｔｈａｔｔｈｅｃｏｎｔｅｎｔｓｏｆｃｙｃｌｉｃｎｕｃｌｅｏｔｉｄｅｓｉｎｐｌａｓｍａａｎｄｃｅｒｅｂｒｏｓｐｉｎａｌｆｌｕｉｄｃｈａｎｇｅｏｂｖｉｏｕｓｌｙｗｈｅｎｄｉｓｏｒｄｅｒｓｏｆｃｅｎｔｒａｌｎｅｒｖｏｕｓｓｙｓｔｅｍｏｃｃｕｒ.Ｔｈｉｓ…     

脑室注射促甲状腺素释放激素对电针镇痛及脑内CAMP含量的影响

<正> 我们曾报道,脑室注射促甲状腺素释放激素(TRH)能对抗电针(EA)和吗啡的镇痛效应,,其机理不清楚。有资料表明,TRH对抗戊巴比妥的镇痛作用是通过影响神经细胞内cAMP实现的,脑内注入     

EFFECT OF ACUPUNCTURE ON EXPERIMENTAL CEREBRAL INFARCTION IN RATS

Ｗｅｈａｖｅｒｅｐｏｒｔｅｄｔｈａｔｓａｔｉｓｆａｃｔｏｒｙｅｆｆｅｃｔｓｗｅｒｅａｃｈｉｅｖｅｄｉｎｔｈｅｔｒｅａｔｍｅｎｔｏｆｃｅｒｅｂｒａｌｉｎｆａｒｃ ｔｉｏｎｍａｉｎｌｙｗｉｔｈ“ＮｉｅＳａｎＺｈｅｎ”ａｎｄ“ＳｉＳｈｅｎＺｈｅｎ”[1 ] .Ａｔｔｈｅｐｒｅｓｅｎｔ ,“ＮｉｅＳａｎＺｈｅｎ”ａｎｄ“ＳｉＳｈｅｎＺｈｅｎ”ａｒｅｗｉｄｅｌｙａｄｏｐｔｅｄｉｎｔｈｅｃｌｉｎｉｃ .Ｉｎｏｒｄｅｒｔｏａｃｑｕｉｒｅｒｅｌｉａｂｌｅｅｘｐｅｒｉｍｅｎｔａｌｄａｔａ ,ｗｅｉｎｖｅｓｔｉｇａｔｅｄｃｈａ…     

营心宁对实验性大鼠心肌缺血时PAgT及NO水平的影响

目的 :探讨中药复方制剂营心宁防治冠心病心绞痛的作用机制。方法 :用腹腔注射垂体后叶素 (Pit)法制备心肌缺血缺氧模型 ,观察营心宁及合心爽 (地尔硫唑 )对实验性心肌缺血大鼠一般情况、心电图、血小板聚集率 (PAgT)及心肌组织一氧化氮 (NO)系统水平的变化的影响。结果 :营心宁组和合心爽组大鼠心肌缺血阳性率分别显著低于造模组 (P<0 05) ,且营心宁组和合心爽组间无显著性差异 (P>0 05)。造模组PAgT比阴性对照组显著升高 (P<0 01) ,营心宁组和合心爽组的PAgT显著低于造模组 (分别为P<0 01,P<0 05) ,但仍高于阴性对照组 ,其中营心宁组PAgT低于合心爽组 ,有显著性意义 (P<0 05)。造模组NO含量及NOS活性明显低于阴性组 (P<0 05) ;而营心宁组和合心爽组的NO含量和NOS活性均比造模组明显升高 (P<0 05)。营心宁组NO系统水平低于合心爽组 ,但二者间无显著性差异 (P>0 05)。结论 :营心宁浸膏可降低实验性大鼠心肌缺血心电图阳性率 ,减轻实验性大鼠心肌缺血程度 ,其机制可能为提高心肌组织中NO含量及NOS活性 ,舒张血管平滑肌 ,缓解冠脉痉挛 ,同时降低体内血小板聚集率 ,缓解血液高凝状态 ,防止血栓形成 ,减轻血瘀程度     

EFFECT OF ACUPUNCTURE ON CEREBRAL IMAGES IN AUTISM CHILDREN

Objective: To observe the effect of acupuncture on images in autism children. Methods: A total of 27 cases of autism children were subjected into this study. By using a SPECT, the cerebral images were collected before and after acupuncture treatment and analyzed according to the recommended methods in 《Clinical Nuclear Medicine》 for assessing the state of blood flow, radioactivity quantity distribution and radioactivity count in bilateral hemispheres. "JIN's three-needling" was employed. The acupuncture treatment was given once every other day, with 4 months being a therapeutic course and an interval of one month between two courses. Results: After acupuncture treatrnent, of the 22 cases, 20 had remarkable improvement and 2 had improvement in cerebral blood flow, with the total effective rate being 90.8%. Before the treatment there were significant differences between the left and right cerebrum (P<0. 001), and between the left and right frontal lobes in radioactive areas (P<0.01); however, after trea     

电针对局灶性脑缺血再灌注大鼠细胞间黏附分子-1表达的影响

目的：研究电针对局灶性脑缺血再灌注大鼠ICAM-1表达的影响。方法：采用SD大鼠80只,随机分为正常对照组、假手术组、模型组、电针组,每组20只。采用大脑中动脉线栓法制备MCAO缺血再灌注模型,神经功能障碍评分参照Zea—Longa评分标准;分别应用免疫组化SABC方法检测脑缺血再灌注大鼠缺血脑区微血管内皮细胞ICAM-1的表达和ELISA法检测外周血中sICAM-1含量及电针对其的影响。结果：脑缺血再灌注后,缺血脑区微血管内皮细胞CAM-1表达水平和外周血中sICAM-1含量均增高（模型组与正常组、假手术组比较P〈0．01）;电针治疗后缺血脑区微血管内皮细胞ICM-1表达水平和外周血中sICAM-1含量下调（电针组与模型组比较P〈0．05）。结论：脑缺血再灌注后缺血脑区微血管内皮细胞释放ICAM-1,对脑组织产生炎性损伤;电针治疗可减少ICAM-1的表达,从而发挥脑保护作用。     

ELECTROACUPUNCTURE AFFECTS CELL APOPTOSIS AND EXPRESSION OF NR2B PROTEIN IN ISCHEMIC CORTEX AFTER PERMANENT CEREBRAL ISCHEMIA IN RATS

Objective: Cerebral ischemia resulting from permanent occlusion of cerebral arteries causes neurological impairments.In the present study, the effects of electroacupuncture on cell apoptosis and expression of NR2 B protein following permanent ischemia were investigated.Methods: Rats were randomly divided into three groups: sham operation group, control group, and electroacupuncture group.Electroacupuncture at Zusanli(ST 36) started 24 h after permanent middle cerebral artery occlusion.Motor performance was measured by the rotarod test.Cell apoptosis was examined using labeling(TUNEL) assay and NR2 B protein expression was examined using immunochemical staining(SP method) in the ischemic cortex.Results: Two weeks after surgery, we performed rotarod tests.Compared with the sham operation group, the animals had significantly worse performance(P0.01) in the control group.Compared with the control group, the animals had significantly worse performance(P0.05) in the electroacupuncture group.NR2 B protein was significantly reduced in the ischemic cortex of rats with electroacupuncture as compared with the rats without electroacupuncture(P0.05).There were less apoptosis cells in the sham operation group.The number of apoptosis cells was significantly decreased in the ischemic cortex of rats in the electroacupuncture group as compared with the control group(P0.05).Conclusion: This study suggests that after permanent brain ischemia, electroacupuncture treatment at Zusanli(ST36) improves motor performance and suppresses cell apoptosis and expression of NR2 B protein in ischemic cortex after permanent cerebral ischemia.