骨肉瘤中CD147、MMP-2的表达和意义

目的：探讨骨肉瘤组织CD147、MMP-2蛋白的表达及意义。方法：应用免疫组织化学染色SP法检N137例骨肉瘤及10例骨软骨瘤CD147和MMPP-2的表达。结果：骨肉瘤中CD147、MMP-2的阳性表达率均明显高于骨软骨瘤;骨肉瘤中CD147、MMP-2的阳性表达与Enneking分期和肺转移有关,且二者的表达呈正相关（P〈0．01）。结论：CD147NMMP-2在骨肉瘤的浸润和转移方面起重要作用,联合检测CD147和MMP-2有望成为判断骨肉瘤预后的新指标。     

RANKL、OPG、TRAIL在骨肉瘤中的表达及其临床意义

目的 探讨骨肉瘤组织中RANKL、OPG、TRAIL蛋白的表达情况.方法 采用免疫组织化学SP法测定RANKL、OPG、TRAIL蛋白在50例骨肉瘤、20例骨软骨瘤中的表达.分析其在骨肉瘤、骨软骨瘤中表达差异及与骨肉瘤临床病理特征之间的关系.结果 OPG在骨肉瘤中的表达明显高于骨软骨瘤(P<0.01);TRAIL在骨肉瘤中的表达明显低于骨软骨瘤(P<0.01).OPG、TRAIL的表达与Enneking临床分期有关(P<0.01).结论 OPG、TRAIL是骨肉瘤发生、发展的标记之一.     

XIAP和Caspase-3在骨肉瘤中的表达及其意义

目的研究骨肉瘤中X相关凋亡抑制蛋白(x chromosome linked inhibitor of apoptosis protein,XIAP)和胱天蛋白酶-3(Caspase-3)的表达及其临床意义。方法应用免疫组化方法检测38例骨肉瘤和10例骨软骨瘤中XI-AP和Caspase-3的阳性表达情况。结果10例骨软骨瘤中,Caspase-3表达7例阳性,XIAP表达均阴性。在骨肉瘤组中Caspase-3阳性表达率18.4%;而XIAP阳性表达率68.4%,显著高于骨软骨瘤(P0.05)。XIAP蛋白的表达与患者的年龄、性别、病理分型、临床分期无相关。骨肉瘤组中,Caspase-3阳性表达率为18.4%,其中低度恶性、组织细胞分化中等G1期骨肉瘤表达明显高于高度恶性、组织细胞分化差的G2期(P0.05)。Caspase-3的表达与患者的年龄、性别不相关。骨肉瘤组中Caspase-3和XIAP表达趋势呈负相关性(P0.05)。结论骨肉瘤组中XI-AP呈高表达,Caspase-3呈低表达;骨软骨瘤中Caspase-3呈高表达,XIAP呈低表达;骨肉瘤组中XIAP和Caspase-3表达趋势呈负相关。     

PTEN和Gaspase-3在骨肉瘤中的表达

目的探讨抑癌基因PTEN蛋白和Caspase-3在骨肉瘤中的表达及在其发生发展中的可能作用.方法用免疫组织化学方法检测PTEN和Caspase-3在38例骨肉瘤及20例骨软骨瘤中的表达.结果PTEN蛋白在骨肉瘤中,表达阳性率为55.3%.在对照组骨软骨瘤中为90.0%,骨肉瘤与骨软骨瘤相比有显著差异(P＜0.01).Caspase-3在骨肉瘤中表达阳性率为52.6%,在对照组骨软骨瘤中为85.0%,骨肉瘤与骨软骨瘤相比有显著差异(P＜0.05).结论PTEN蛋白和Caspase-3与骨肉瘤的发生有关.     

人骨肉瘤组织中抑癌基因P27蛋白的表达

目的探讨人骨肉瘤组织中P27蛋白的表达及其与骨肉瘤发生、发展和病理分型的关系.方法采用免疫组化SP法,检测10例骨软骨瘤组织和30例骨肉瘤组织(骨母细胞型14例,软骨母细胞型5例,纤维母细胞型8例,混合型3例;高分化7例,低分化23例)中P27蛋白的表达情况.结果骨肉瘤组织中P27蛋白阳性表达率为33.33%(10/30),低于骨软骨瘤中的80%(8/10)(P＜0.05).骨肉瘤高分化组P27蛋白阳性表达率为71.43%(5/7),高于低分化组的21.74%(5/23)(P＜0.05).骨肉瘤各病理亚型间P27蛋白阳性表达率差异无统计学意义(P＞0.05).结论P27蛋白表达与骨肉瘤发生、发展密切相关,可作为判断骨肉瘤恶性程度的一个指标.     

Survivin蛋白在人骨肉瘤、骨软骨瘤和正常骨组织中表达的初步研究

目的 探讨Survivin蛋白在人骨肉瘤、骨软骨瘤和正常骨组织上的表达及其相关性.方法 利用SP免疫组织化学技术检测骨肉瘤、骨软骨瘤和正常骨组织Survivin蛋白的表达情况,并采用Bresalier染色评分法记录结果后进行方差分析.结果 Survivin蛋白在正常骨组织中检测不到;Survivin蛋白在骨软骨瘤中呈低至中度表达;Survivin蛋白在骨肉瘤中呈高度表达;Survivin蛋白的表达在骨肉瘤、骨软骨瘤和正常骨组织相互之间均差异有显著性(P＜0.05);在骨肉瘤中,Ⅲ期骨肉瘤的Survivin蛋白的表达强度与ⅡA期、ⅡB期差异有显著性(P＜0.05),而ⅡA期与ⅡB期的骨肉瘤的Survivin蛋白的表达强度差异没有显著性(P＞0.05);在骨肉瘤中,Survivin蛋白的表达与患者的性别、年龄及组织学分型未见明显相关性.结论 证实Survivin蛋白在正常骨组织中不表达,在骨肉瘤中的表达高于骨软骨瘤,提示Survivin蛋白的表达与骨肿瘤的恶性程度有关;在骨肉瘤的Ennecking外科分期中,ⅡA、ⅡB期的survivin蛋白表达低于Ⅲ期,提示survivin蛋白的表达与骨肉瘤患者的浸润、转移相关.     

SPK、S1P蛋白在骨肉瘤中的表达及其临床意义

目的：探讨鞘氨醇激酶1(SPK1)、1-磷酸鞘氨醇(S1P)在骨肉瘤中的表达及其临床意义。方法：选择行手术治疗的73例骨肉瘤病人作为观察对象,选择同期骨软骨瘤手术切除标本31例作为对照,免疫组织化学法检测骨肉瘤组织与软骨瘤组织中SPK1、S1P蛋白表达水平,分析SPK1、S1P表达水平与骨肉瘤临床病理特征的关系,Kaplan-Meier生存曲线分析SPK1、S1P表达与骨肉瘤病人生存率的关系。结果：骨肉瘤组织SPK1阳性表达率为68.49%,高于软骨瘤组织中阳性表达率16.13%(P<0.01);骨肉瘤组织S1P阳性表达率为58.90%,高于软骨瘤组织中阳性表达率22.58%(P<0.01);SPK1、S1P在Eneeking分期为Ⅱ～Ⅲ期和肺转移的病人中阳性表达率较高(P<0.05～P<0.01);SPK1、S1P在病人的不同性别、年龄、肿瘤直径、发病部位、病理类型的阳性表达率差异无统计学意义(P>0.05);SPK1阳性表达组3年生存率低于SPK1阴性表达组(P<0.05);S1P阳性表达组3年生存率低于S1P阴性表达组(P<0.05)。结...     

非小细胞肺癌GRIM-19和STAT3的表达及临床意义

目的：探讨非小细胞肺癌（NSCLC）GRIM-19和STAT3蛋白的表达及与临床病理因素的关系。方法：应用免疫组化ABC法检测GRIM-19和STAT3在49例NSCLC及20例正常肺组织中的表达。结果：NSCLC中GRIM-19和STAT3的阳性率分别为55.1%（27/49）和65.3%（32/49）,正常肺组织中表达阳性率分别为95%（19/20）和0%（0/20）,NSCLC与正常肺组织比较差异有显著性（P〈0.01）。GRIM-19的阳性表达与肿瘤组织的临床分期有关,Ⅰ-Ⅱ期的表达明显高于Ⅲ＋Ⅳ期（P〈0.05）;STAT3的阳性表达与肿瘤组织的临床分期及是否有淋巴结转移有关,Ⅰ期、无淋巴结转移分别低于Ⅱ期、Ⅲ＋Ⅳ期和有淋巴结转移者（P〈0.05）。NSCLC中GRIM-19和STAT3的表达呈显著负相关（r=-0.647,P〈0.05）。结论：NSCLC中GRIM-19低表达与STAT3高表达共存,通过提高GRIM-19的表达抑制或阻断STAT3信号转导通路或能成为治疗肺癌的新途径。     

P糖蛋白在骨肉瘤中的表达及其临床意义

目的探讨p-gp在骨肉瘤组织中的表达及其临床意义。方法应用免疫组化方法（S-P法）测定p-gp在36例骨肉瘤和17例骨软骨瘤组织中的表达,并随访骨肉瘤患者生存时间。36例骨肉瘤中男19例,女17例,年龄平均19.9岁。Enneking分期ⅡA3例,ⅡB27例,ⅢB6例,并发病理性骨折7例。结果 36例骨肉瘤随访时间平均3.5a,其中存活3a及以上者13例,存活3a以下者23例,死亡病例均因骨肉瘤远处转移全身衰竭致死。36例骨肉瘤p-gp阳性表达率为50.0%,显著高于骨软骨瘤组,有显著性差异（P〈0.01）。p-gp表达在不同Enneking外科分期组间无显著性差异（P〉0.05）,在生存时间3a以上组及3a以下组间有显著性差异（P〈0.01）。结论 p-gp表达可能与骨肉瘤作为高度恶性肿瘤的生物学行为有关,对骨肉瘤患者的预后评估具有一定价值。     

P14ARF和PCNA在骨肉瘤中表达及其意义

目的:探讨P14ARF和增殖细胞核抗原(PCNA)在骨肉瘤中的表达及其意义。方法:采用免疫组织化学SP法检测52例骨肉瘤中的P14ARF和PCNA表达,并以15例骨软骨瘤作为对照,与临床病理参数进行比较分析。结果:P14ARF在骨肉瘤的阳性表达率为26.9%(14/52),在骨软骨瘤为73.3%(11/15),差异有统计学意义(P<0.01);PCNA在骨肉瘤的阳性表达率为84.6%(44/52),在骨软骨瘤为13.3%(2/15),差异亦有统计学意义(P<0.01);P14ARF和PCNA在骨肉瘤的表达与患者年龄、性别、组织类型及民族均无相关性(P>0.05)。结论:P14ARF和PCNA表达与骨肉瘤的发生、发展有关,其可作为诊断骨肉瘤及评价其预后的辅助指标。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.