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Use of an immunoglobulin G avidity test to discriminate between primary and secondary dengue virus infections 下载免费PDF全文
de Souza VA Fernandes S Araújo ES Tateno AF Oliveira OM Oliveira RR Pannuti CS 《Journal of clinical microbiology》2004,42(4):1782-1784
An enzyme-linked immunosorbent assay-based immunoglobulin G (IgG) antibody avidity test was evaluated by using sera from 57 patients with acute dengue infection. Overall, 55 of 57 patients were correctly classified (27 of 27 with primary dengue and 28 of 30 with secondary dengue). We conclude that the IgG avidity test can be useful for differentiating between acute, primary, and secondary dengue infections. 相似文献
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Liang-Quan Zhang Fu-Rong Zhao Zhi-Gang Liu Wei-Li Kong Heng Wang Yun Ouyang Huan-Bin Liang Chao-Yi Zhang Hai-Tao Qi Chang-Li Huang Si-Hu Guo Gui-Hong Zhang 《Archives of virology》2012,157(12):2383-2388
Hepatitis E virus (HEV) is an enteric pathogen of humans and animals, and pigs have been considered an important reservoir of this virus. Recent evidence has indicated the cross-species transmission of hepatitis E virus (HEV) from pigs to humans, causing zoonosis, mostly via consumption of uncooked or undercooked animal meat/viscera. In this study, we have developed a one-step RT-LAMP assay for rapid detection of swine HEV. Specific primer sets targeting the ORF3 gene were designed. The sensitivity of the RT-LAMP assay was 101 copies/??l of RNA template, which was tenfold higher than that of RT-nPCR. The specificity of this assay was demonstrated by the lack of amplification of DNA/RNA from other swine viruses. Furthermore, a total of 41 bile samples were subjected to RT-LAMP and RT-nPCR. Eighteen positive samples were detected by RT-nPCR, while 36 positive samples were detected by RT-LAMP, indicating that the sensitivity of the RT-LAMP assay was higher than that of the conventional RT-nPCR assay. The RT-LAMP assay reported here may be used for diagnosis of swine HEV, not only in laboratories but also under field conditions. 相似文献
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Development of a reverse transcription multiplex real-time PCR for the detection and genotyping of classical swine fever virus 总被引:1,自引:0,他引:1
Yu-Liang Huang Victor Fei Pang Chu-Hsiang Pan Tsu-Han Chen Ming-Hwa Jong Tien-Shine Huang Chian-Ren Jeng 《Journal of virological methods》2009,160(1-2):111-118
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