Antisperm Antibodies on the Surface of Spermatozoa before Ejaculation from Vasectomized Men

Itisknownthattherewereantispermantibodies(AsAbs)intheseraandsemenaftervasectomyorvasovasotomy"--".In1978ModeratorandModeraterlalraisedaquestionofwhetherAsAbscancoattheepididymalspermatozoaaftervasectomy,orexistonthesurfaceoftheepididymalspermatozoabeforeejaculation.Unforturnately,sofartherehasbeennoreportabouttheAsAbsonthehumanspermatozoaoftheepididymisorproximalvasdeferensaftervasectomy,becauseitisverydifficulttoobtainthehumanspermatozoabeforeejaculation(SBE).Nowthetechniqueofcannulationo…     

Evaluation of Sperm Parameters of Infertile Men with Retrograde Ejaculation

Normal ejaculation results in the antegrade emission and expulsion of sperm, and is an essential prerequisite for natural reproduction. However, congenital deficiency of or damage42 to the bladder neck, spinal cord injury, diabetes, and prostatic surgery …     

Study on the Fluorescent Localization of Seminal Vesicle Secretion Protein Ⅱ on the Surface of Rat Sperm

The binding of seminal vesicle secretion protein Ⅱ (SVP) to rat epididymal sperm surface was studied by means of an indirect immunofluorescent antibody technique. The rat epididymal sperms were incubated with purified SVP Ⅱ then treated with rabbit anti-SVP Ⅱ serum and goat anti-rabbit lgG conjugated to fluorescein isothiocyanate. It was found that the whole sperm surface was coated with fluorescence under the fluorescence microscope, and the fluorescence in the sperm head was the brightest. The conclusion might be therefore that SVP Ⅱ had some ability to bind the epididymal sperm surface and may be one of the components of sperm coating antigen in rats. In addition, it was also demonstrated by immunoabsorption and immunocross-reaction tests that SVP Ⅱ was tissue-specific antigen and there was no cross-reaction with other purified SVP.     

Effect of Anti-KDR Antibody on the Proliferation of Hemangioma Vascular Endothelial Cells in vitro

The suppressive effect of anti-KDR antibody against VEGF on proliferation of hemangioma-derived vascular endothelial cells(HVECs) was investigated.HVECs from one case of hemangioma in proliferative phase were cultured.Both primary culture and sub-culture were conducted in M199 medium.The HVECs of passage 3 were divided into 4 groups based on the concentrations of anti-KDR antibody.Cell count was performed and inhibitory rate of HVECs was measured before and 9 days after interference.The results showed that the number of HVECs in the anti-KDR antibody-treated groups was significantly decreased and the inhibitory rate of HVECs by anti-KDR antibody(50,10 and 2 μg/mL) was 84%,63% and 39% respectively at 9th day after interference,with the difference being significant.In the control group,the number of HVECs was increased significantly.In was concluded that the anti-KDR antibody could suppress the activity of VEGF through blocking the KDR,indicating the potential clinical applications of anti-KDR antibody in the treatment of hemangioma.     

宫腔冲洗液抗精子抗体检测及其对宫腔内人工授精的影响

0　引言　抗精子抗体可导致女性不孕 ,目前临床上主要检测女性外周血中的抗精子抗体 ,而血中的抗精子抗体不孕的关系并不密切 [1 ] .对抗精子抗体阳性者的治疗方法之一为宫腔内人工授精 [2 ] .但我们发现 ,如果宫颈粘液中抗精子抗体阳性 ,宫腔内人工授精的结果很不理想 ,是否宫腔内也存在抗精子抗体不得而知 .因而我们采用宫腔冲洗的方法 ,检测了宫腔内的抗精子抗体 .1　对象和方法1.1　对象　不孕症组为本院门诊不孕症专科患者 ,共计 6 0对夫妇 ,女方年龄 2 4～ 36 (平均 2 8.9)岁 .不孕年限 2～ 7a,平均 3.4 a,其中原发不孕 36例 ,继发不…     

男性早泄诊疗进展综述

早泄是男性常见的性功能障碍。对早泄的认识分为神经官能症和身心障碍阶段、后天习得行为阶段、神经生物学和精神药理学阶段以及制药公司阶段。射精的生理过程需要交感神经、躯体神经和多种递质参与。目前早泄的发病机制尚未完全明确,有些疾病,如甲状腺功能亢进,也会引起早泄症状。对于继发于其他疾病产生的早泄症状,应积极治疗原发病。作为原发病,仅有少数数据支持生物学和心理学方面的理论,包括阴茎超敏感反应、焦虑、伴侣关系问题和5-HT受体功能紊乱等。研究表明,心理-行为治疗、α-1受体阻滞剂、局部麻醉、磷酸二酯酶-5(PDE-5)抑制剂和选择性5-羟色胺再摄取抑制剂(SSRIs)均不同程度的对早泄有效。尽管早泄的发病机理尚未完全明确,但是,生理和心理方面的因素均对早泄的发生产生影响。因此,在早泄的治疗上,不仅要关注患者生理方面阴道内潜伏射精时间的改善,也要关注心理方面的改善。     

输精管结扎家兔外周血巨噬细胞功能的实验研究

体重２．５～３．０ｋｇ日本大耳白兔，分为输精管结扎组（ＶＧ）和假手术组（ＳＯＧ）。术后４、８、１２、１８、２２月经耳静脉采血，玻璃平皿吸附法纯化巨噬细胞，经ＬＰＳ诱生，测巨噬细胞培养上清白细胞介素１（ＩＬ－１）活性，及血浆肿瘤坏死因子（ＴＮＦ）和溶菌酶含量。结果表明，ＶＧ８月ＩＬ－１活性和ＴＮＦ、溶菌酶含量均明显高于ＳＯＧ８月（Ｐ＜０．０２，＜０．０ｌ，＜０．０５），其它各月检测结果组间比较均无显著差异（Ｐ＞０。０５）。ＩＬ－１活性和溶菌酶含量呈明显的正相关关系（ｒ＝０．５７６，Ｐ＜０．００１）     

抗精子抗体的间接免疫荧光测定

本文探索了间接免疫荧光技术检测抗精子抗体的有关问题。输精管结扎术后抗精子抗体的测定,应选择附睾精子作抗原,其最适保存条件是将精子悬液置于-20℃,或用乙醇固定后保存于4℃冰箱中,乙醇固定不仅可增强精子抗原性的显示,而且对其抗原性具有保护作用。本方法所用的附睾精子抗原不须进行其他处理;用精子抗原免疫异种动物,抗精子抗体产生早,效价高,而免疫同种动物则抗原性极弱;抗精子抗体的特异性免疫荧光类型以顶体特异性荧光染色为主,根据不同稀释倍数的待测血清所显示的荧光强度,可判断血清抗精子抗体的效价。     

弱精子症患者精子中DKKL1的表达

目的探讨精子顶体相关基因（DKKL1）在弱精子症患者精子中的表达特征及临床意义。方法收集正常男性和弱精子症 患者精液,各取10 μL精液用全自动计算机辅助精液分析系统（CASA）对精液进行常规分析。为了避免白细胞和生精细胞对结 果的影响,剩余精液采用4个梯度的Percoll法离心分离和纯化精子。Trizol法提取精子RNA,实时荧光定量PCR从基因水平检 测DKKL1基因在正常人和弱精子症患者精子中的表达差异;最后采用Western blot方法从蛋白水平检测DKKL1蛋白在正常人 和弱精子症患者精子中的表达差异。结果精液常规分析结果显示,弱精子症和正常人两组之间的年龄、精液体积、精子浓度和 正常精子形态比例均没有统计学差异（P>0.05）。与正常对照组相比,弱精子症组PR 和PR+NP 精子的比例显著降低（P< 0.01）。实时荧光定量PCR结果显示,与正常对照组相比,DKKL1 mRNA在弱精子症患者精子中的表达量降低11.1倍。此外, Western blot结果显示,弱精子症组DKKL1蛋白的表达量与正常对照组相比,降低了2.4倍,差异具有统计学意义（P<0.01）,该 结果与实时荧光定量PCR结果一致。结论DKKL1在弱精子症患者精子中的表达水平明显下降,表明其与精子运动密切相关, 是导致弱精子症发生的原因之一。     

3992例男性体表测量数据的分析

目的:描述南通市3 992名男性体表测量数据分布特征及实际的营养状况,并为制定预防政策、提高人群身体健康状况提供科学依据。方法:通过询问、人体测量等方法获得研究变量,进行统计指标描述。结果:(1)本市男性目标人群的超重以及肥胖现患率分别为39.55%和11.12%;(2)评价本市≥30～<50岁以及≥60岁男性人群居民营养水平时,建议将体质量指数(boby mass index,BMI)正常区间设为19.0～23.9 kg/m2。结论:本市男性居民营养水平优于全省平均水平,并提示成年男性人群中≥40～<60岁是发生肥胖及相关疾病的高危年龄段,建议对该部分人群提出有针对性的防治策略。     

沙眼衣原体感染与血清中抗精子抗体相互关系的研究

目的 探讨男性不育患者CT感染与血清中AsAb的关系。方法 应用聚合酶链反应(PCR)与ELISA法对177例男性不育患者分别进行CT和AsAb检测。结果 CT阳性者AsAb的阳性率(27.9％)明显高于CT阴性者的AsAb阳性率(8.3％,X2=12.2,P<0.01)。结论 沙眼衣原体感染可增加AsAb的产生,可能与男性不育有关。     

生殖道解脲脲原体感染与抗精子抗体相互关系的研究

目的　探讨不育症患者Uu与AsAb的关系。方法　应用分离培养法与ELISA法对临床 2 0 5例不育症患者分别进行了Uu和AsAb检测。结果　Uu感染组和Uu未感染组AsAb阳性率 ,女性分别为34.2 9%和 10 .0 0 % (χ2 =9.39,P <0 .0 1) ,男性分别为 2 5.58%和 4 .76 % (χ2 =7.11,P <0 .0 1)。结论　Uu感染可增加AsAb的产生 ,可能与不育有关。