米诺环素局部用药对牙周炎患者龈沟液中超氧化物歧化酶(SOD)活性的影响

目的比较应用米诺环素软膏治疗前后牙周炎患者临床指标及龈沟液中超氧化物歧化酶(SOD)活性的变化.方法成人牙周炎患者30例,分成2组,实验组常规牙周治疗+米诺环素软膏,对照组仅给予常规牙周治疗,记录两组治疗前后牙龈指数(GI)、牙周探诊深度(PD)、附着丧失(AL);并用放射免疫法检测两组龈沟液SOD活性.结果(1)治疗后,试验组牙龈指数(GI)、牙周探诊深度(PD)均明显低于对照组(P＜0.05,P＜0.01);而治疗前两组比较,上述指标未见显著差别.(2)治疗前,实验组龈沟液SOD活性与对照组比较无显著差别(P＞0.05),而治疗后,实验组龈沟液SOD活性显著高于对照组(P＜0.05).结论米诺环素局部用药可显著降低牙周炎患者牙龈指数(GI)、牙周探诊深度(PD)并提高龈沟液SOD活性.     

盐酸米诺环素缓释抗菌软膏修复牙周牙髓联合病变

背景:关于盐酸米诺环素对慢性牙周炎、牙周脓肿等的辅助治疗作用已有较多报道,但有关盐酸米诺环素在牙周牙髓联合病变中的详细研究,目前国内报道较少。目的:探讨缓释型盐酸米诺环素在牙周牙髓联合病变临床治疗中应用的效果。方法:选择68例(68颗)牙周牙髓联合病变患者进行牙周机械治疗联合根管治疗,按患者意愿分3组进行牙周治疗,实验组(n=24)牙周机械治疗后局部注入盐酸米诺环素,阳性对照组(n=24)牙周机械治疗后局部注入碘甘油,阴性对照组(n=20)牙周机械治疗后未注入药物,3组均于牙周机械治疗后1个月复诊,比较治疗前后牙周探诊深度、附着丧失和龈沟出血指数的差异。结果与结论:牙周机械治疗1个月后,3组牙周探诊深度、附着丧失和牙龈出血指数均较治疗前明显改善(P0.05);实验组与阳性对照组牙周探诊深度和龈沟出血指数优于阴性对照组(P0.05),实验组牙周探诊深度和龈沟出血指数优于阳性对照组(P0.05)。表明在牙周牙髓联合病变的临床治疗中,除了采取牙周机械治疗和根管治疗,局部应用缓释型盐酸米诺环素能有效降低牙周袋深度,改善牙龈炎症状况,巩固临床治疗效果。     

盐酸米诺环素软膏治疗中重度慢性牙周炎的临床疗效

目的 探讨盐酸米诺环素软膏治疗中重度慢性牙周炎的临床疗效。方法 选取2017年2月~2019年3月我院口腔科收治的中重度慢性牙周炎患者136例,随机分为观察组和对照组,各68例。对照组予碘甘油治疗,观察组以盐酸米诺环素软膏治疗。比较两组出血指数（BI）、牙龈指数（GI）、探诊深度（PD）、牙齿松动度（TM）及临床效果。结果 观察组PD、BI、GI、TM分别为（2.31±0.21）mm、（0.78±0.23）分、（0.66±0.18）分、（0.17±0.08）mm,均低于对照组的（3.20±0.43）mm、（1.22±0.36）分、（1.37±0.37）分、（0.30±0.15）mm,差异有统计学意义（P＜0.05）;观察组治疗总有效率高于对照组,差异有统计学意义（P＜0.05）。结论 对中重度慢性牙周炎患者局部予以盐酸米诺环素软膏疗效显著,可有效降低患者牙龈出血及牙齿松动情况。     

慢性牙周炎治疗前后患牙龈沟液中IL-8和TNF-α水平变化比较

目的:探讨慢性牙周炎患者治疗前后患牙龈沟液中IL-8和TNF-α水平的变化。方法:选择本院就诊的慢性牙周炎患者96例为研究组和健康体检的牙周健康者50例作为对照组,研究组在基础治疗前后收集龈沟液(GCF)并记录相关的临床指标,包括探诊深度(PD)、临床附着丧失(CAL)和探诊出血点数(BOP),应用ELISA法测定两组龈沟液内IL-8和TNF-α的含量。结果:研究组治疗前牙周临床指标PLI、PD、SBI和AL均明显高于对照组,经过牙周基础治疗后,各指标均较治疗前显著下降(P0.05)。研究组治疗前和治疗后龈沟液IL-8和TNF-α水平均显著高于对照组(P0.05)。研究组治疗后龈沟液IL-8和TNF-α水平均明显较治疗前降低(P0.05)。研究组治疗前龈沟液中IL-8的水平与PD呈显著正相关(r=0.486,P0.05),TNF-α水平与SBI、PD呈显著正相关(r=0.495,0.648,P0.05)。结论:慢性牙周炎疾病发生发展与龈沟液中IL-8和TNF-α浓度之间存在着密切联系,且反映了慢性牙周炎的严重程度。     

结合四环素和抗炎剂的牙周治疗对早发性牙周炎抗牙龈卟啉菌抗体水平的影响

目的：评价结合四环素和抗炎剂的牙周治疗对早发性牙周炎抗牙龈卟啉菌抗体IgG水平的影响。 方法： 研究对象由12名早发性牙周炎患者、16名成年牙周炎患者和12名牙周健康者组成。牙周炎患者接受常规牙周治疗，并口服四环素和消炎痛，牙周治疗前及治疗3个月后常规临床检查、记录牙周探诊深度和牙周附着水平及牙周探诊出血指数，并测定治疗前后患者血清抗牙龈卟啉菌抗体IgG滴度的变化。 结果： 牙周治疗后，牙周探诊深度、附着水平和牙周出血指数均有显著改善，血清抗牙龈卟啉菌抗体滴度明显下降。 结论： 结合四环素和消炎痛的机械性牙周治疗对早发性牙周炎可获得满意的疗效。血清抗牙龈卟啉菌抗体水平对评估早发性牙周炎的预后具有重要意义。     

盐酸米诺环素软膏与甲硝唑对种植体周围炎抑制作用的 临床研究

目的 对比局部运用盐酸米诺环素软膏和口服甲硝唑治疗种植体周围炎的临床效果。方法 选取2016年3月~2018年5月来我院口腔门诊就诊的种植体周围炎患者36例,在对种植体进行龈上洁治、龈下刮治后,随机分为盐酸米诺环素软膏组和口服甲硝唑组,每组18例。盐酸米诺环素软膏组局部运用盐酸米诺环素软膏治疗,口服甲硝唑组采用口服甲硝唑治疗。对比基线、治疗后4周和8周改良菌斑指数（mPLI）、改良龈沟出血指数（mSBI）和探诊深度（PD）参数。结果 治疗后4周及8周,两组患者mPLI、mSBI以及PD参数均低于基线,差异具有统计学意义（P＜0.05）;盐酸米诺环素软膏组治疗后4周及8周的mPLI、mSBI以及PD参数均低于口服甲硝唑组[4周:mPLI（0.69±0.47）vs （1.46±0.33）,mSBI（0.62±0.41） vs （1.16±0.38）,PD（2.47±0.37）mm vs （3.52±0.54）mm;8周:mPLI（0.98±0.39）vs（1.52±0.30）,mSBI（0.86±0.45）vs（1.26±0.43）,PD（2.93±0.50）mm vs （3.46±0.46）mm],差异具有统计学意义（P＜0.05）。结论 盐酸米诺环素软膏和甲硝唑治疗种植体周围炎有确切的疗效,应用盐酸米诺环素软膏效果明显优于口服甲硝唑,局部使用药物可以实现短期的大幅优化。     

牙周基础治疗对糖尿病患者血清和龈沟液中肿瘤坏死因子-α的影响

目的 观察牙周基础治疗对2型糖尿病伴牙周炎患者血清及龈沟液中肿瘤坏死因子-α(TNF-α)的浓度、临床牙周状态、血糖控制的影响.方法 选取2型糖尿病伴牙周炎患者60例,随机分做牙周基础治疗组(观察组)和不做牙周基础治疗组(对照组),每组各30人.分别在治疗前、治疗后1个月和3个月记录所有患者牙周临床指数:探诊深度(PD),附着丧失(AL)及菌斑指数(PLI),并检测糖化血红蛋白(HbAlc)及血清及龈沟液中TNF-α的含量.结果 观察组中PD、PLI和龈沟液中TNF-α含量在治疗后1个月和3个月时均显著降低(P＜0.05),AL和血清中HbAlc及TNF-α含量仅在治疗后3个月显著降低(P＜0.05).对照组的PD,AL,PLI,HbAlc,龈沟液及血清中TNF-α的差异无统计学意义(P＞0.05).结论 牙周基础治疗有助于2型糖尿病伴牙周炎患者的血糖控制,牙周状态改变和血清及龈沟液中TNF-α含量下降.     

螺旋霉素联合替硝唑局部给药治疗慢性牙周炎的临床疗效观察

目的：观察螺旋霉素联合替硝唑治疗慢性牙周炎的临床疗效。方法将我院收治的82例慢性牙周炎患者按照随机对照原则分为螺旋霉素联合替硝唑治疗组(观察组)和对照组单纯替硝唑治疗组(对照组),每组各41例,比较两组患者治疗后的牙龈指数(GI)、菌斑指数(PLI)、龈沟出血指数(SBI)以及牙周袋探诊深度。结果观察组患者治疗后的GI、PLI、SBI以及牙周袋探诊深度均较对照组患者有显著改善,组间差异有统计学意义(P<0.05)。结论螺旋霉素联合替硝唑治疗慢性牙周炎疗效确切,值得临床进一步推广使用。     

盐酸米诺环素软膏对慢性牙周炎患者的疗效

目的:探讨盐酸米诺环素软膏对慢性牙周炎患者牙周指数、炎症因子水平的影响.方法:收集本院2018年4月至2020年4月收治的160例慢性牙周炎患者进行双盲法分组,对照组患者(n=80)采用生理盐水冲洗联合碘甘油流入治疗,1次?w-1,实验组患者(n=80)合用盐酸米诺环素软膏置入的方式治疗,2次?w-1,治疗1 m后比较两组患者治疗前、后牙周指数,采用酶联免疫吸附法测定血清炎症因子白介素-6(Interleukin-6,IL-6)和C反应蛋白(C-reactive protein,CRP)水平,同时观察用药安全性.结果:与对照组相比,实验组的治疗总有效率明显提高(P<0.05);与治疗前相比,各治疗组患者血清CRP、IL-6水平、牙周指数均明显降低(P<0.05),其中实验组更为显著(P<0.05);两组不良反应无差异(P>0.05).结论:盐酸米诺环素软膏对慢性牙周炎的治疗效果理想.     

牙周组织再生术联合口腔正畸治疗牙周炎的效果观察

目的 探讨牙周组织再生术联合口腔正畸对牙周炎患者的治疗效果。方法 选取2016年10月~2019年3月在我院诊治的牙周炎患者84例,采用随机数字表法分为常规组与研究组,各42例。常规组采用传统牙周组织再生术治疗,研究组在常规组基础上联合口腔正畸治疗。比较两组口腔相关指标（牙龈附着水平、牙龈指数、牙周探诊深度、牙菌斑指数）、治疗总有效率及不良反应发生率。结果 研究组治疗后牙龈附着水平、牙龈指数、牙周探诊深度、牙菌斑指数均优于常规组,差异具有统计学意义（P＜0.05）;研究组治疗总有效率（95.24%）高于对照组（78.57%）,差异具有统计学意义（P＜0.05）;研究组不良反应发生率（7.14%）低于常规组（23.80%）,差异具有统计学意义（P＜0.05）。结论 牙周组织再生术联合口腔正畸治疗,可优化牙周炎患者的牙龈附着水平、牙龈指数等口腔相关指标,降低不良反应发生率,值得临床应用。     

2010年广州某医疗中心儿童夏季细菌性腹泻的病原学调查

目的研究广州地区小儿夏季细菌性腹泻的病原菌分布。方法采集2010年5～7月广州市妇女儿童医疗中心珠江新城院区腹泻患儿的大便标本进行常规病原菌的分离培养,通过生化反应和血清凝集试验进行鉴定和分型,并使用金标法对空肠弯曲菌抗原进行检测。结果从110份标本中检出44株病原菌,检出率为40.0%。其中致病性大肠埃希菌17株,2岁以下患儿检出15株;空肠弯曲菌12株,2岁以下患儿检出10株;沙门菌6株;念珠菌纯生长6株;产气荚膜杆菌3株。结论广州地区夏季儿童细菌性腹泻的主要病原菌是致病性大肠埃希菌及空肠弯曲菌,两者的易感人群以2岁以下婴幼儿为主。     

MiRNA调控肿瘤细胞中P-gP表达的研究进展

微小RNA（miRNA）是一类高度保守的非编码单链RNA分子,与靶基因mRNA3’端非编码区结合,抑制mRNA的翻译,促进靶基因降解。而这种调控机制与很多肿瘤细胞中P型糖蛋白（P,glycoprotein,p-gp）的表达有着密切的联系。p-gp是与耐药相关的跨膜蛋白,过表达可导致肿瘤细胞产生多药耐药（multidrugresistance,MDR）。就miRNA的生物学特性、p-gP的功能进行综述,关系。肿瘤MDR是临床化学治疗失败的主要原因,本文并重点介绍其调控肿瘤细胞中P-gP表达和MDR的     

Changes in activity of neutral proteases in tissues of ground squirrels in the dynamics of hibernation

Total activities of neutral proteases in the cerebral, hepatic, and myocardial tissues of ground squirrel vary during hibernation: in autumn (before hibernation) activities of the enzymes in the brain and myocardium start increasing, while in the liver they do not change. A common feature for all tissues is minimum activity of active neutral proteases in the middle of hibernation month 1 bout, while the maximum activity is recorded before awakening. Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 146, No. 9, pp. 278–280, September, 2008     

293细胞检测致癌物的应用研究

目的 探讨应用293细胞对致癌物进行细胞转化及致瘤性等研究,为检测可疑致癌物提供实验依据.方法 应用微囊藻毒素LR(Microcystin LR,MC-La)对293细胞进行转化培养,取转化后的细胞进行软琼脂克隆实验、血清依赖性实验以及免疫缺陷小鼠体内致瘤实验来验证MC-LR转化的293细胞的致癌性.结果 293细胞在MC-LR的作用下,逐渐表现出转化细胞的特性:血清依赖程度显著降低,在软琼脂培养基中锚着独立生长并形成细胞集落,在SCID小鼠体内形成浸润性肿瘤组织.结论 293细胞易于培养并对环境致癌物敏感,可用于可疑致癌物的检测.     

西藏藏族青少年皮纹密度

目的:获得藏族皮纹密度的基本参数。方法:在知情同意原则的基础上,拓印拉萨市6所大、中、小学藏族学生的掌指纹,观察皮纹密度,并测量身高、体质量、掌长、手长、手宽5项指标。结果:藏族青少年的皮纹密度随年龄的增加而降低,并有性别差异。结论:藏族青少年的皮纹密度在青春发育过程中变化较大,其皮纹密度大于同年龄组汉族。     

贵州黔东南地区育龄人群地中海贫血基因类型分析

目的了解贵州黔东南州育龄人群地中海贫血基因类型及其分布。方法 2017年1月~2019年3月对930例疑似地中海贫血的育龄人群采用PCR结合导流杂交进行α、β地中海贫血基因检测。结果 930例病例中检出地中海贫血422例,检出率45.38%,α地中海贫血193例(20.75%),β地中海贫血212例(22.80%),α复合β地中海贫血23例(1.83%)。12种α地中海贫血基因类型主要为--SEA/αα(67.87%)、-α3.7/αα(9.84%)和-α4.2/αα(7.25%),8种β地中海贫血基因类型以CD41-42/N(61.79%)、CD17/N(26.89%)和IVS-Ⅱ-654/N(6.13%)常见,8种α合并β地中海贫血基因类型主要为-α3.7/CD41-42(41.19%)和--SEA/CD41-42(23.53%)。结论贵州黔东南州β地中海贫血检出率高于α地中海贫血,主要基因类型为CD4142/N和--SEA/αα。     

Factor of selectivity in organization of complex behavior in primates

Selectivity in the activity of the mammalian brain is examined on the basis of an analysis of a substantial experimental material obtained in our laboratory during the investigation of the conditioned reflex and delayed behavior in lower and higher primates.Translated from Fiziologicheskii Zhurnal SSSR imeni I. M. Sechenova, Vol. 73, No. 2, pp. 269– 276, February, 1987.     

Induction of high frequency activity in the somatosensory thalamus of rats in vivo results in long-term potentiation of responses in SI cortex

Summary Extracellular single-unit techniques were employed to record unitary activity simultaneously from the thalamic ventral posterior medial (VPM) nucleus and the ipsilateral primary somatosensory cortex of adult rats. Cross-correlation analysis triggered by the spontaneous firing of thalamocortical relay neurons in VPM and the discharge of layer IV neurons in the corresponding ipsilateral cortical barrel indicated that the paired-units included in this study were strongly correlated in their activity. The baseline responses of highly correlated cortical/thalamic pairs to a 10 ms deflection of a vibrissa on the contralateral side were measured using poststimulus time histograms. After establishing the baseline response, high frequency activity in VPM was induced in one of two ways: i) direct electrical stimulation of thalamic neurons or ii) whisker stimulation in the presence of bicuculline methiodide (BIC) released near the thalamic neurons. Both methods resulted in a conditioning stimulus (CS) paradigm consisting of bursts of high-frequency activity (50–100 Hz) with an inter-burst interval of 150 ms (7 Hz). Almost immediately following the presentation of the CS, the response of layer IV cortical neurons to vibrissa stimulation increased by 37–62% over baseline values, which was maintained after the effects of BIC had worn off in VPM. This enhancement in the response of the cortical neurons was not accompanied by a concomitant increase in the thalamic responses. Thus, these results strongly suggest that the potentiation first occurred at the thalamocortical synapse.     

Role of Thrombin in Activation of Neurons in Rat Hippocampus

The effect of thrombin, an agonist of proteinase-activated receptor (PAR) family, was studied on cultured rat hippocampal neurons. Thrombin in a concentration range of 1 pM - 10 nM induced a transitory dose-dependent increase in intracellular free calcium concentration. Involvement of PAR1 in neural response to thrombin was corroborated in experiments with TFLLRN, a selective synthetic peptide agonist of these receptors. In a calcium-free medium and after treatment with cyclopiazonic acid (inhibitor of Ca(2+)-ATPase in the endoplasmic reticulum) activation of PAR not only mobilized Ca(2+) from intracellular stores, but also induced Ca(2+) entry into the cells. Thrombin decreased Ca(2+) signal triggered by activation of NMDA-subtype glutamate receptors.     

Role of Nitric Oxide in Prevention of Cognitive Disorders in Neurodegenerative Brain Injuries in Rats

NO synthesis disturbances play an important role in the development of neurodegenerative damage in Alzheimer disease. We previously showed that adaptation to intermittent hypobaric hypoxia prevents cognitive disturbances in rats with experimental Alzheimer disease [6]. Here we evaluated the role of NO in cognitive disorders and development of adaptive protection during experimental Alzheimer disease. Adaptation to hypoxia in rats was performed in a hypobaric pressure chamber at a simulated altitude of 4000 m (4 h per day for 14 days). Alzheimer disease was simulated by bilateral injections of a toxic fragment of β-amyloid (25–35) into n. basalis magnocellularis. For evaluation of the role of NO in the development and prevention of memory disorders, the rats received intraperitoneally either NO-synthase inhibitor N^ω-nitro-L-arginin (L-NNA, 20 mg/kg, every other day for 14 days) or NO-donor dinitrosyl iron complex (200 μg/kg daily for 14 days). NO-synthase inhibitor potentiated the damaging effect of β-amyloid, abolished the protective effect of adaptation to hypoxia, and produced memory disorders in rats similar to those observed during experimental Alzheimer disease. In contrast, the increase in NO level in the body provided by injections of the NO-donor produced a protective effect against memory disorders caused by β-amyloid similar to that induced by adaptation to hypoxia. We concluded that reduced NO production in the organism plays an important role in the development of cognitive disorders produced by injections of β-amyloid, while prevention of NO deficit by administration of NO-donors or nonpharmacological stimulation of NO synthesis can provide a protective effect in experimental Alzheimer disease. Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 146, No. 10, pp. 371–375, October, 2008