C-erbB-2和nm23-H_1产物在结肠癌中表达的意义

目的：探讨ＣｅｒｂＢ２和ｎｍ２３Ｈ１产物在结肠癌中表达的临床意义。方法：用ＬＳＡＢ法对６４例结肠癌标本的ＣｅｒｂＢ２和ｎｍ２３Ｈ１基因产物的表达进行研究。结果：ＣｅｒｂＢ２蛋白表达与组织类型及淋巴结转移有相关性（Ｐ＜０．０５）;ｎｍ２３Ｈ１表达与淋巴结转移及Ｄｕｋｅｓ分期有相关性（Ｐ＜０．０５）;在结肠癌中两基因产物表达呈正相关（ｒ＝０．４３３Ｐ＜０．０５）。结论：ＣｅｒｂＢ２和ｎｍ２３Ｈ１在结肠癌发生发展中起协同作用,同时检测两种基因产物表达可作为评判结肠癌的临床特征的重要指标。     

nm23基因在膀胱移行细胞癌中表达的临床意义

目的：探讨nm23基因产物与膀胱移行细胞癌生物学行为之间的关系。方法：应用免疫化学方法检测45例膀胱移行细胞癌细胞nm23基因表达,结果：nm23蛋白弥散于胞浆及包膜,nm23蛋白表达与膀胱癌分级呈正相关,但与分期无关,有转移性癌组织nm23表达明显高于无转移癌组织（P＜0．001）,结论：nm23基因表达是一个判断膀胱移行细胞癌恶性程度有价值的指标。     

nm23基因产物在大肠癌中的表达及其临床意义

应用免疫组织化学方法对８３例不同分期的大肠癌及其正常粘膜组织和１２例大肠腺瘤进行转移抑制基因ｎｍ２３表达产物的研究，结果显示：全部大肠正常粘膜均为弱阳性表达，大肠腺瘤虽全部阳性，但弱阳性占７５％，而大肠癌的阳性率为９１．６％，且其中５３％ｎｍ２３基因产物的表达较其正常粘膜为高；淋巴结转移组的ｎｍ２３基因产物表达较未转移组为低（Ｐ＜０．０１）；ｎｍ２３基因产物的表达与大肠癌的分化呈正相关（Ｐ＜０．０１），分化好的其表达强，而分化差的其表达弱甚至阴性ｎｍ２３基因产物的表达与大肠癌患者的预后呈正相关（Ｐ＜０．０５），表达强的，预后较好。综合表明：ｎｍ２３在大肠癌肿瘤的发生、发展、转移及预后上有重要的作用。     

nm23在人乳腺癌中的表达及其临床意义

目的 :研究转移抑制基因nm2 3在人乳腺癌中的表达及其临床预后价值。方法 :应用免疫组化S P法检测 6 5例临床及随访资料完整的乳腺癌患者标本中nm2 3的表达情况 ,用Cox回归分析其预后意义。结果 :nm2 3阳性表达率为 5 5 .4 %。全组总的五年生存率及五年无瘤生存率分别为 6 3.1%及 5 2 .3%。nm2 3表达与远处转移负相关 (P =0 .0 0 0 3) ,与生存率及无瘤生病率呈正相关 (P =0 .0 0 34,P =0 .0 0 0 1)。多因素Cox回归分析显示 ,腋淋巴结状态及nm2 3不同表达 2项指标反映乳腺癌预后情况 ,危险度分别为 3.10 4 6及 0 .370 4。结论 :nm2 3是仅次于乳腺癌腋淋巴结状态的独立的预后指标 ,检测nm2 3有助于筛选出具有高复发及转移危险的患者 ,从而指导临床治疗     

nm23/NDPK在胰腺癌中表达和临床意义

目的　探讨胰腺癌中nm2 3表达水平与胰腺癌淋巴结转移、临床分期、神经浸润及预后的关系。方法　应用免疫组织化学法对 3 0例胰腺癌中nm2 3表达水平进行研究。结果　胰腺癌中nm2 3的表达率为 46.67% ,明显高于正常胰腺组织 ;胰腺癌中nm2 3的表达水平与胰腺癌淋巴转移 ,临床分期密切相关 (P <0 .0 5、P <0 .0 1) ,与神经浸润无关 (P>0 .0 5 ) ;nm2 3阳性表达组预后明显低于阴性表达组 (P <0 .0 0 5 )。结论　nm2 3的表达与胰腺癌恶性度及预后相关。     

nm23基因在人结肠癌中的表达及其与预后的关系

ｎｍ２３基因在人结肠癌中的表达及其与预后的关系王锡山王艳颖杨为戈肖梅①董新舒赵家宏哈尔滨医科大学第三临床医学院（哈尔滨市１５００４０）我们采用免疫组织化学（ＳＰ）方法，对１０１例人结肠癌进行ｎｍ２３基因表达研究，探讨其与结肠癌生物学行为以及预后的关系...     

nm23基因在膀胱移行细胞癌中表达的临床意义

目的 :探讨nm2 3基因产物与膀胱移行细胞癌生物学行为之间的关系。方法 :应用免疫组织化学方法检测 4 5例膀胱移行细胞癌组织nm2 3基因表达。结果 :nm2 3蛋白弥散于胞浆及胞膜 ,nm2 3蛋白表达与膀胱癌分级呈正相关 ,但与分期无关 ,有转移性癌组织nm2 3表达明显高于无转移癌组织 (P<0 .0 0 1)。结论 :nm2 3基因表达是一个判断膀胱移行细胞癌恶性程度有价值的指标     

nm23基因在乳腺癌中的表达及临床意义

目的研究nm23基因在乳腺癌中的表达及临床意义。方法应用免疫组化染色对65例资料完整有7年随访结果的原发性乳腺癌nm23基因蛋白染色进行检测。结果65例患者nm23基因高表达36例,低表达29例,转移和非转移组nm23基因低与高表达有显著性差异(P＜0.005),nm23低表达易发生转移。生存5年以上和5年以下的两组患者nm23表达有显著性差异(P＜0.005)。nm23表达与年龄、病理类型、临床分期相关。结论nm23基因表达和乳腺癌的转移、预后有关。联合考虑nm23基因的表达高低和强度,才能更合理的判定转移,并可筛选出具有潜在高转移危险的患者。     

nm23基因、雌孕激素受体在乳腺癌中的表达及临床意义

目的研究nm23基因,ER、PR在乳腺癌中的表达及临床意义.方法应用免疫组化染色对65例资料完整有7年随访结果的原发性乳腺癌nm23基因表达,ER、PR进行检测.结果65例患者nm23基因高表达36例,低表达29例.转移和非转移组nm23基因低与高表达有显著性差异(P<0.005),nm23低表达易发生转移.生存5年以下和5年以上的两组患者nm23的表达水平有显著性差异(P<0.005),高表达者比低表达者预后好;本研究未发现nm23表达与年龄,病理类型、临床分期的相关性;65例患者ER(+)PR(+)者33例,ER(-)、PR(-)者16例,两组之间nm23高表达和低表达存在着显著性差异(P<0.005).结论nm23基因表达和乳腺癌的转移呈负相关,和预后呈正相关.联合考虑nm23基因的表达高低和强度,才能更合理的判定转移,并可筛选出具有潜在高转移危险的患者;在判断转移和预后方面,ER(+)PR(+)与nm23基因高表达之间存在着正相关性,ER(-)PR(-)和nm23基因低表达之间存在着正相关性.     

Expression of nm23-H1 gene product in nasopharyngeal carcinoma and its clinical significance

Objective: The nm23 gene is one of the tumor metastatic suppressor genes. The expression of nm23-H1 has been reported to be inversely associated with metastatic potentiality in a number of human carcinomas, including breast, colorectal, gastric, hepatocellular and gallbladder carcinomas. In this study, the immunohisto-chemical staining of nm23-H1 protein in human naso-pharyngeal carcinoma (NPC) was examined, and the relationship between nm23-H1 and both metastasis and prognosis of patients with NPC was also investigated. Methods: Routine LSAB immunohistochemistry with the nm23-H1 monoclonal murine antibody was employed to study the expression of nm23-H1 protein in 95 paraffin-embedded specimens of NPC treated at our hospital. The clinical pathologic data and results of follow-up were also retrieved. Comparisons between patients with and without expression of nm23-H1 protein with respect to metastasis, loco-regional recurrence and survival were performed using Log rank test. Multivariate prognostic analyses were performed by using Cox’s regression model. Results: Nm23-H1 negative expressive tumors were associated with a higher incidence of lymph-node metastasis (86.7%) than those of nm23-H1 positive (48.6%,P<0.01). Nm23-Hl negative expressive tumors were associated with a high incidence of recurrence and distant metastasis after radiotherapy (P<0.05). A significant association was found between expression of nm23-H1 and prognosis (P<0.01). The expression of nm23-H1 indicated favorable prognosis. Conclusion: It was suggested that nm23-H1 negative expression was significantly associated with lymph-node metastasis, recurrence and distant metastasis. Nm23-H1 may have value for predicting the prognosis of NPC.     

Prognostic significance of nm23-H1 expression in oral squamous cell carcinoma

Recent studies indicated nm23-H1 played a role in cancer progression. Therefore, we investigated clinical significance of nm23-H1 expression in oral squamous cell carcinoma (OSCC). In total, 86 OSCC specimens were immunohistochemically stained with nm23-H1-specific monoclonal antibodies. Immunohistochemical staining of nm23-H1 was confirmed by immunoblotting. The relations between nm23-H1 expression and clinicopathologic variables were evaluated by chi(2) analysis. As increased size of primary tumour could escalate metastatic potential and the data of patients at the late T stage might confound statistical analyses, we thus paid special attention to 54 patients at the early T stage of OSCC. Statistical difference of survival was compared by a log-rank test. Immunohistochemically, nm23-H1 expression was detected in 48.8% (42 out of 86) of tumorous specimens. It positively correlated with larger primary tumour size (P=0.03) and inversely with cigarette-smoking habit (P=0.042). In patients at the early T stage, decreased nm23 expression was associated with increased incidence of lymph node metastasis (P=0.004) and indicated poor survival (P=0.014). Tumour nm23-H1 expression is a prognostic factor for predicting better survival in OSCC patients at the early T stage, which may reflect antimetastatic potential of nm23. Therefore, modulation of nm23-H1 expression in cancer cells can provide a novel possibility of improving therapeutic strategy at this stage. In addition, our results further indicated cigarette smoking could aggravate the extent of nm23-H1 expression and possibly disease progression of OSCC patients.     

nm23-H1基因表达与鼻咽癌的早发转移

目的探讨nm23-H1基因产物的表达与鼻咽癌早发转移的关系.方法应用免疫组化S-P法对95例鼻咽癌组织蜡块进行nm23-H1检测.结果nm23-H1的阳性表达率为47.4%(45/95),早发转移组nm23-H1阳性率(26.7%)低于无转移组(60.0%),差异有统计学意义(P＜0.05).nm23-H1表达与临床分期无关.结论nm23-H1阴性表达与鼻咽癌早发转移有关,nm23-H1的表达水平可能是预测中晚期鼻咽癌远处转移的一个重要的生物学指标.     

THE HIGH-LEVEL EXPRESSION OF nm23(NDP)GENE IN NPC

WE have studied the expression of nm23(NDP)in 50cases nasopharyngeal biopsies with anti-nm23(NDP)antibodies. As a result, the NDP positive rate innasopharyngeal carcinoma(NPC)(95.54%) markedlyincreased (P<0.05), as compared with that in the normalnasopharyngeal epithelia (50.00-60.00%) and lympho-cytes (52.00%). There were cytoplasmic type, nucleustype and mixed cytoplasmonucleus type according to NDP,location in a cell Their positive rates were 64.44%,15.56%, and 20.00%, respectively in nasopharyngealcarcinoma. The expression of NDP had no relation withcervical lymphometastases in NPC, and the NDP positiverates had no significance between bilateral cervicallymphometastases and unilateral(P<0.05). But the NDPexpression had most relation with the NPC staging. Theexpression rate and the intensity in Ⅲ or Ⅳ stagepatients were markedly higher than that in Ⅱ stage. Itpoints out that the high-level expression of NDP hadrelation with the rapid cellular proliferation in NPC, andit may indicate the bad prognos     

SNCG、 nm23在胃癌中的表达及临床意义

目的:研究胃癌中SNCG,nm23的表达及其临床意义,并进一步探讨它们在胃癌中表达的相关性.方法: 采用免疫组化法观察60例胃癌及20例胃正常黏膜石蜡标本中SNCG、 nm23的表达情况,实验数据用SPSS12.0软件包进行统计.结果: 在胃癌中SNCG蛋白阳性率65%,高于周围正常组织(P<0.05).nm23在胃癌组织中表达阳性率为55%,低于周围正常组织(P<0.01).SNCG、 nm23的表达与肿瘤浸润深度、淋巴结转移及肿瘤临床分期均相关(P<0.05).SNCG与nm23在胃癌中的表达呈负相关(P<0.05)).结论: SNCG、 nm23蛋白的表达与胃癌发生、发展和浸润转移相关.胃癌组织中SNCG的表达与nm23的表达呈负相关,二者的检测有助于胃癌浸润转移的预测,对判断患者预后有重要参考价值.