复方三嗪芦丁片中利血平及盐酸异丙嗪含量测定

目的 建立高效液相色谱法测定复方三嗪芦丁片中利血平及盐酸异丙嗪的含量.方法 采用C18(200min×4.6mm,5μm)色谱柱,乙腈-水(用高氯酸调节pH至2.15±0.05)(41:59)为流动相,检测波长为268nm.结果 利血平在3.84～15.36μg·ml-1浓度范围内呈良好线性关系(r=0.9996),平均回收率为99.4%,RSD=0.82%(n=9);盐酸异丙嗪在0.252～1.008mg·ml-1浓度范围内呈良好线性关系(r=0.9998),平均回收率为99.8%,RSD=0.98%(n=9).结论 本方法简便、准确、可靠.     

HPLC法测定盐酸普鲁卡因葡萄糖注射液中盐酸普鲁卡因和对氨基苯甲酸的含量

目的:用HPLC法同时测定盐酸普鲁卡因葡萄糖注射液中盐酸普鲁卡因和对氨基苯甲酸含量.方法:色谱柱为Nova-PakC18(150 mm×4.0 mm,4μm);流动相为甲醇-10mmol·L-1 NaH2PO4溶液(用磷酸调pH6.48,含2 mml·L-1三乙胺)(30:70,V/V);流速为0.7 ml·min-1;检测波长为287 nm,柱温30℃.结果:盐酸普鲁卡因浓度在O.1～1.0 mg·ml-1范围内线性关系良好(r=0.998 9),平均加样回收率98.9%(n=3,RSD=1.4%);对氨基苯甲酸浓度在1～10μg·ml-1范围内线性关系良好(r=0.999 6),平均加样回收率99.2%(n=3,RSD=1.6%).结论:该方法准确、直观、便于盐酸普鲁卡因葡萄糖注射液质量控制.     

HPLC法测定复方利血平片中氢氯噻嗪、盐酸异丙嗪和利血平的含量

目的 建立HPLC法测定复方利血平片中氢氯噻嗪、盐酸异丙嗪和利血平的含量。方法采用C18柱为固定相（150mm×4．6mm×5μm）,0．01mol·L^-1庚烷磺酸钠：乙腈：三乙胺（53：47：0．1）,用磷酸溶液调节pH至5．5为流动相,检测波长为230～380nm。结果氢氯噻嗪在0．1934—2．9013g·L^-1浓度范围内线性关系良好,r=0．9999,平均加样回收率为100．44％,RSD为0．54％（n=6）;盐酸异丙嗪在0．135～2．025g·L^-1浓度范围内线性关系良好,r=1．0000,平均加样回收率为100．25％,RSD为0．18％（n=6）;利血平在1．965—29．475mg·L^-1浓度范围内线性关系良好,r=1．0000,平均加样回收率为100．73％,RSD为0．47％（n=6）。结论该方法具有操作简便、结果准确的优点,可用于复方利血平片中氢氯噻嗪、盐酸异丙嗪和利血平的含量。     

复方双嗪利血平片中利血平溶出度测定方法的研究

目的 建立复方双嗪利血平片中利血平的溶出度测定方法.方法 依照<中国药典>2005年版二部附录溶出度项下第三法,以正丙醇-0.1mol·L-1盐酸(3:7)为溶出介质,转速为100r·min-1,高效液相色谱法检测,检测波长为268nm.结果 在0.064～0.256μg·ml-1范围内(r=0.9997),浓度与峰面积呈良好的线性关系,平均回收率为100.2%,RSD为1.0%,(n=9).结论 该法操作简便、准确可靠.     

HPLC法同时测定盐酸异丙肾上腺素注射液和盐酸肾上腺素注射液的含量及有关物质

目的:建立测定盐酸异丙肾上腺素注射液和盐酸肾上腺素注射液的含量及有关物质的方法。方法:采用高效液相色谱法,色谱柱:Welch Materials C18色谱柱(4.6mm×150mm,5μm);流动相:甲醇-乙腈-庚烷磺酸钠溶液(1:1:8);检测波长:280nm;流速:2.0ml·min-1;柱温:30℃。结果:盐酸异丙肾上腺素在10～40μg.ml-1质量浓度范围内与峰面积呈良好的线性关系,r=0.9999;高、中、低3种浓度的平均回收率为100.5%,RSD为0.76%(n=9)。盐酸异丙肾上腺素的最小检测限为40.66ng·ml-1。盐酸肾上腺素在60～240μg·ml-1质量浓度范围内与峰面积呈良好的线性关系,r=0.9996;高、中、低3种浓度的平均回收率为100.2%,RSD为0.60%(n=9)。盐酸肾上腺素的最小检测限为44.12ng·ml-1。结论:该法简便、快速、准确、灵敏度高,重现性好,适用于盐酸异丙肾上腺素注射液和盐酸肾上腺素注射液的含量测定,也可用于有关物质检查。     

紫外分光光度法测定复方盐酸利多卡因注射液的含量

目的建立紫外分光光度法测定复方盐酸利多卡因注射液中盐酸利多卡因和薄荷脑的含量。方法盐酸利多卡因采用以水为空白,检测波长为261 nm。薄荷脑采用以阴性对照液加显色剂为空白,检测波长为507 nm。结果盐酸利多卡因在200μg.ml-1~450μg.ml-1浓度范围内,呈良好线性关系;平均回收率为102.1%,RSD为1.80%(n=9)。薄荷脑在1.6μg.ml-1~6.4μg.ml-1浓度范围内,呈良好线性关系;平均回收率为98.5%,RSD为1.70%(n=9)。结论本方法简便、快捷、准确、可靠。     

高效液相色谱法测定脑塞通丸中人参皂苷Rg1、Re、Rb1的含量

目的建立脑塞通丸的含量测定方法.方法采用高效液相色谱法.色谱柱Agilent Technologies ZORBAX ExtendC18(250 mm×4.6 mm,5μm),流动相乙腈-0.05%磷酸溶液梯度洗脱,流速1.0 ml·min-1,检测波长203 nm,柱温35℃.结果人参皂苷Rg1在8.516～425.8μg·ml-1范围内线性关系良好(r=0.999 9),平均加样回收率为97.6%,RSD=1.7%(n=6);人参皂苷Re在20.06～1003μg·ml-1范围内线性关系良好(r=0.999 7),平均加样回收率为97.6%,RSD=2.0%(n=6);人参皂苷Rb1在19.456～972.8μg·ml-1范围内线性关系良好(r=0.999 7),平均加样回收率为96.8%,RSD=1.3%(n=6).结论该方法简便易行,结果准确可靠,可适用于脑塞通丸的质量控制.     

紫外分光光度法测定复方替硝唑凝胶中替硝唑和盐酸利多卡因的含量

目的:建立紫外分光光度法测定复方替硝唑凝胶中替硝唑和盐酸利多卡因含量的方法.方法:分别采用单波长法和双波长法不经分离直接测定替硝唑和盐酸利多卡因的含量,替硝唑的测定波长为317 nm,盐酸利多卡因的测定波长为263 nm,参比波长为360 nm.结果:替硝唑在浓度为6～16 μg·ml-1范围内线性关系良好,r=0.999 8,平均回收率为101.0%,RSD为0.9%(n=9).盐酸利多卡因在浓度为0.100~0.501 mg·ml-1范围内线性关系良好,r=0.999 9,平均回收率为100.9%,RSD为0.6%(n=9).结论:该法操作简便,准确性较好,可用于直接测定复方替硝唑凝胶中替硝唑和盐酸利多卡因的含量.     

气相色谱法测定盐酸金刚乙胺中3种有关物质

目的：采用气相色谱法测定盐酸金刚乙胺中已知有关物质杂质B、杂质A和杂质C的含量。方法：样品经氢氧化钠碱化处理,正己烷提取,以金刚烷为内标物质,采用分流进样法,以RESTEK Rtx-5毛细管色谱柱（30 m×0.32 mm,0.25μm）为分析柱进行分离测定。结果：杂质A在9.996～199.92μg·ml-1范围内具有良好线性关系（r=0.999 6）,平均回收率为98.99%（RSD=0.9%,n=9）;杂质B在10.02～200.48μg·ml-1范围内具有良好线性关系（r=0.999 2）,平均回收率为99.21%（RSD=0.9%,n=9）;杂质C在10.14～202.80μg·ml-1范围内具有良好线性关系（r=0.999 5）,平均回收率为100.03%（RSD=1.2%,n=9）。结论：本法灵敏度高、结果准确、重复性好,可用于盐酸金刚乙胺有关物质的测定。     

RP-HPLC同时测定四季三黄软胶囊中黄芩苷和盐酸小檗碱的含量

目的建立RP-HPLC法同时测定四季三黄软胶囊中黄芩苷和盐酸小檗碱的含量.方法采用色谱柱为hypersil BDSC18(250 mm×4.6 mm,5μm),以乙睛-0.5%三乙胺水溶液(磷酸调pH至3.1)(2575)为流动相,检测波长265 nm,流速1.0ml·min-1.结果黄芩苷在1.6-206 lag·ml-1范围内,线性关系良好(r=0.999 9),平均回收率为100.3%.RSD=1.7%(n=6);盐酸小檗碱在1.6-208μg·m1-1范围内,线性关系良好(r=0.999 9),平均回收率为100.8%,RSD=1.6%(n=6).结论该方法结果准确可靠,重现性好,可用于四季三黄软胶囊的质量控制.     

复方利血平片质量标准的研究

目的：提高完善复方利血平片的质量标准.方法：采用化学方法建立了处方中利血平、氢氯噻嗪、盐酸异丙嗪、维生素B1维生素B6、KCl、泛酸钙、三硅酸镁的鉴别项,并对其作空白对照,专属性良好;采用HPLC法建立了利血平、氢氯噻嗪、盐酸异丙嗪三个成分的含量测定项目.结果：定性定量准确、专属性强、重现性好.结论：提高后的质量标准可有效地控制该产品的内在质量.     

Mechanism of ulcerogenic activity of reserpine in albino rats

Reserpine-induced gastric ulceration was significantly prevented by α-adrenoceptor blockers but not by propanolol or by bilateral adrenalectomy. Intracerebroventricular (i.c.v.) administration of reserpine as well as tetrabenazine failed to induce ulceration in albino rats. Pretreatment with 6-hydroxydopamine or atropine methyl nitrate protected the animals from the ulcerogenic activity of reserpine.     

The action of reserpine on teleost melanophores

Reserpine caused darkening of both pencil fish and angelfish, this effect lasted for at least 10 and 25 days respectively.The aggregation of pigment granules within the melanophores evoked by stimulation of the nerves supplying the melanophores was inhibited by reserpine in both species. Reserpine caused a lowering of the catecholamine content of pencil fish skin. The time course both these effects paralleled the time course of darkening. The response of the melanophores of the angelfish to adrenaline, noradrenaline, dopamine, and melatonin was not affected by treatment with reserpine for 3 days. However, reserpine treatment for 14 days induced a marked sensitivity to noradrenaline. Reserpine partially abolished the response to tyramine in both pencil fish and angelfish. Small doses of noradrenaline and adrenaline given to reserpine-treated angelfish partially restored the response of the melanophores to nerve stimulation. No action of ACTH, MSH, nor direct effect of reserpine could be demonstrated on melanophores of either species. It was concluded that reserpine acts on the melanophores of pencil fish and angelfish in an indirect way by depletion of catecholamines from the sympathetic nerves supplying the melanophores.     

Effects of denervation and reserpine on nexuses in the rat vas deferens

Permanganate-fixed vasa deferentia from rats were examined for the presence of nexal-like contacts by electron microscopy. There was a significantly greater incidence of nexuses (2×) in chronically denervated tissues (5–7 days) but not in tissues from reserpine-pretreated animals (1.0 mg/kg/day for 5–7 days). The results suggest that an increase in nexal regions may not be a general feature of postjunctional supersensitivity but rather may contribute to other denervation-induced changes in contractile response.     

Loss of body weight as a predictor of reserpine-induced amine depletion.

Loss of body weight was proved to be a convenient and reliable way to predict the degree of reserpine-induced amine depletion after i.p. injections of reserpine (2.5, 5 and 10 mg/kg) and it thus helps reduce variability. Two populations of animals were sharply distinguished lying on either side of the 5% cutoff point within the first 24 hr. One group (approximately 20% of all animals) lost less than 5% of their body weight, showed absence of physiological and behavioural effects, a moderate 5-HT depletion and a moderate 5-HIAA increase. These animals were called 'partially reserpinized'. Another group always lost 5% or more of body weight, exhibited the reserpine syndrome as well as a larger extent of 5-HT depletion and 5-HIAA increase. This group comprised 'reserpinized' rats. There were significant correlations between the percent weight loss and the degree of 5-HT depletion and 5-HIAA increase in the group of 'partially reserpinized' animals. The two groups differed also in the time course of amine changes. Dose had no effect in the differentiation of the two groups but did affect the extent of amine depletion. When reserpine was given i.v. or s.c., all animals were 'reserpinized'. The phenomenon of partial reserpinization is discussed.     

Lack of effect of chronically administered thyrotropin-releasing hormone (TRH) on regional rat brain tyrosine hydroxylase activity.

Chronic treatment of adult male rats with TRH (1 or 10 mg/kg IP) for 5 or 9 days failed to alter the activity of tyrosine hydroxylase (TH), the enzyme regulating the rate-limiting step in catecholamine biosynthesis. In contrast, as previously described, chronic reserpine administration (0.5 mg/kg IP: 9 days) resulted in a significant rise in TH activity in midbrain, hypothalamus, pons-medulla and forebrain. These results suggest that the enhanced brain norepinephrine turnover reported to occur after treatment with TRH is not due to synthesis of new TH enzyme protein.     

The effect of some hallucinogenic and other drugs on the temperature of reserpinized mice

Seven hallucinogenic and 9 centrally acting non-hallucinogenic drugs were studied for their calorigenic effects on reserpine pre-treated mice. With the exception of Ditran and mescaline, all the hallucinogens had a significant calorigenic effect. Of the other drugs studied, methylamphetamine and amphetamine, dimethoxyphenylethylamine, desmethylimipramine, amitryptyline and chlorpromizine also had a marked calorigenic action. Both -methyl-m-tyrosine, and its active metabolite metaraminol, had a significant calorigenic action in reserpinized mice even though they did not significantly affect the body temperature of otherwise untreated animals. The calorigenic effect of -methyl-m-tyrosine was significantly antagonized by phencyclidine, ketamine, LSD and mescaline; the other hallucinogens (harmine, Ditran and p-bromethylamphetamine) were without effect. Of the other drugs tested, only propranolol, desmethylimipramine and amitriptyline significantly antagonized the calorigenic effect of -methyl-m-tyrosine in reserpinized mice. The possible mechanism of action of the drugs studied is tentatively discussed.     

丁螺环酮加强戊四氮致小鼠惊厥作用

新型非苯二氮(艹卓)类抗焦虑剂丁螺环酮(Bus)(1～10 mg·kg~1)使戊四氮(PTZ)致惊厥作用的CD_(50)下降12～37％,具有剂量依赖性和时间效应关系.色氨酸羟化酶抑制剂对氯苯丙氨酸(250 mg·kg~1)和单胺耗竭剂利血平(2mg·kg~1)均可增强PTZ致惊厥作用,但同时减弱Bus的增强作用.而α_2受体激动剂可乐定和赛拉嗪以及DA受体激动剂阿朴吗啡,拮抗剂氟哌啶醇均不影响Bus的增强作用.结果表明,Bus的增强作用有5-HT能神经元的参与.     

加钖果宁的镇痛作用及其作用机制

野花椒系芸香科植物(Zanthoxylum simulans Hance),其根皮有止痛及局麻作用。其水溶性生物碱有肌松作用,曾用于外科手术作为中麻辅助用药。常志青从中分离到加钖果宁[(—)edulinine,简称加钖碱,下同],并发现有镇痛及中枢抑制作用。本文进一步证明其镇痛作用及其作用机制与阿片受体有关。     

HPLC法测定利血平注射液中利血平的含量

目的:建立以高效液相色谱法测定利血平注射液中利血平含量的方法。方法:色谱柱为Venusil XBP-C18,流动相为甲醇性范-水围(为524:～481,6pHμg=·(m3L±-(10.r2=))0,.9检99测1波);长平为均2回68收n率m为,流9速9.6为9%1,mRLS·Dm=in0-.91,8柱%(温n为=29)8。℃结,进论样:本量方为法10准μ确L。、可结靠果、:重利复血性平好检,测可浓用度于该线制剂的含量测定。