克山病病区粮对大鼠~(65)Zn代谢影响的实验研究

本实验研究以克山病病区与非病区粮饲养大鼠8周,进行~(65)Zn 示踪实验,观察2日和7日时血液、心、肝、脾、肾、肌肉及骨骼对~(65)Zn(ZnCl_2)的吸收情况。结果表明,病区粮组大鼠在两次观察时间,各种组织脏器对~(65)Zn 的吸收率普遍高于非病区组,其中以肝、肾、心脏和肌肉的差异尤为显著。揭示病区与非病区粮之间存在着差异,即病区粮能造成大鼠体内锌不足或缺乏。认为这一现象在克山病发病方面的作用值得进一步探讨。     

克山病病区粮对大鼠心肝自由基代谢的影响

用克山病病区产粮喂养大鼠,观察动物体内自由基代谢状况,结果表明病区粮组大鼠心肌和肝组织氧自由基含量和脂质过氧化物均显著高于非病区粮组和合成饲料组。本实验发现,病区粮组大鼠肝组织 ESR 谱线出现一特殊的 X 峰,g 值为2.043,其性质和意义有待进一步探索     

钙对模拟克山病偏食喂养大鼠自由基代谢的影响

本文研究了克山病偏食饲料及在这种饲料中补育钙和硒对大鼠自由基代谢的影响。结果表明，这种克山病偏食饲料中除低硒外低钙特别突出，所饲大鼠血清钙水平、全血及心肌谷城肽过氧化物酶活性，过氧化氢酶、超氧化物歧化酶等自由基清除酶明显低于常规食组；心肌及血清中脂质过氧化物水平明显升高。在克山病偏食饲料中单饲料中单纯补钙可明显改善大鼠生长状态和降低心肌及血清中脂质过氧化物含量，但ＧＳＨ－ＰＸ水平无增高，血清ＣＡＴ     

克山病病区粮对大鼠~(54)Mn代谢影响的实验研究

本项研究采用云南克山病病区及非病区粮喂养大鼠,进行~(54)Mn示踪代谢实验。观察锰在动物体内吸收、分布、排除特点及其差异,以期为病因与发病机理研究提供线索。病区与非病区粮组饲料以大米、小麦为主,配     

克山病病区粮饲养大鼠血液流变学的改变

测定了饲克山病病区粮大鼠血液流变学指标。结果表明，病区粮组大鼠红细胞压积、红细胞聚集性、红细胞聚集指数明显增高，而红细胞变形性明显降低。结果提示，血液流变性能的改变可能参与克山病的发病环节。     

亚硝酸钠对饲克山病病区粮大鼠血液流变学影响

以低硒（Ｓｅ）低维生素Ｅ（ＶＥ）为克山病病区粮饲养大鼠１０周，用腹腔注射亚硝酸钠引起急性缺氧的方法，结果表明，病区粮组大鼠红细胞变形性，ＧＳＨ－Ｐｘ，Ｃｕ，Ｚｎ－ＳＯＤ活力降低，血清ＣＫ，ＬＰＯ含量增高，补Ｓｅ和ＶＥ可使上述变化逆转。结果提示，红细胞变形性，抗氧化能力和自由基代谢障碍，是亚硝酸钠与Ｓｅ和ＶＥ缺乏协同造成心肌损害的作用环节。     

克山病病区粮饲养大鼠对~(51)铬代谢的示踪实验研究

本文以克山病病区与非病区粮饲养大鼠14周,进行~(51)Cr 示踪代谢实验,观察血液、心、肝、肾等脏器~(51)Cr(Na_2CrO_4)的存留量及粪、尿内~(51)Cr 的排泄量,其结果表明,病区粮饲养组大鼠在两次观察时间里,血液、肾脏~(51)Cr 存留量均高于非病区粮组,差异显著;心脏,肝脏~(51)Cr 存留量,两组比较差异不明显。粪、尿内~(51)Cr 排泄量,非病区粮组均高于病区粮组。提示病区饲料与非病区饲料,喂养大鼠后,动物对 Cr 代谢出现一定差异,认为进一步观察其它组织脏器铬代谢水平,在探讨克山病病因研究方面有一定意义。     

硒和维生素E对克山病病区粮喂饲大鼠心肌损伤的影响

本实验在克山病病区粮食中补充硒和维生素E(VE)或单独补充硒喂饲大鼠,和单纯喂饲病区粮大鼠比较,联合补充硒和VE可明显减轻亚硝酸钠引起的大鼠心肌损伤;硒加VE组大鼠红细胞高铁血红蛋白还原酶活性明显升高,其可使亚硝酸盐氧化血红蛋白形成的高铁血红蛋白迅速还原,从而减轻机体缺氧状态;硒和VE可使大鼠心肌脂质过氧化产物丙二醛含量明显减少,减轻了病区粮喂饲大鼠心肌损伤;硒加VE使大鼠血清甲状腺激素(T_3、T_4)和心肌呼吸酶(CCO、SDH)活性维持在低水平,表明其纠正了病区粮喂养大鼠心肌能量代谢紊乱,使代偿增加的血清T_3、T_4和心肌CCO、SDH活性降低。和病区粮组大鼠比,单纯加硒组大鼠除心肌谷胱甘肽过氧化物酶活性升高外,其心肌损伤未明显减轻。 上述结果提示,联合补充硒和VE在预防克山病发病上可能会有更好效果。     

铬对克山病病区粮喂养大鼠的甲状腺激素及cAMP水平的影响

为探讨铬对心肌损伤时Ｔ３、Ｔ４及ｃＡＭＰ水平的影响，应用克山病病区低硒粮、加铬、加硒及加铬加硒喂养大鼠，以放射免疫法测定血清Ｔ３、Ｔ４及心肌ｃＡＭＰ含量。结果显示病区粮组Ｔ３、Ｔ４及ｃＡＭＰ水平较对照组下降（Ｐ〈０．０１），加铬、加硒及加铬加硒组较对照组无差异（Ｐ〉０．０５），较病区粮组升高（Ｐ〈０．０５￣０．０１）。铬、硒能维持低硒心肌损伤时甲状腺激素及ｃＡＭＰ的正常代谢。     

克山病病区粮喂养之大白鼠肝脏甲状腺素5′-脱单碘酶活性的改变

测定了以克山病病区粮和非病区粮喂养之大白鼠肝脏 T_4 5′-脱单碘酶活性和巯基含量。结果表明病区粮组动物肝脏 T_4 5′-脱单碘酶活性下降,其机制可能与低硒和巯基含量不足有关。提出肝脏损伤在克山病发病过程中可能起重要作用,值得深入研究。     

克山病病区玉米及酱块对大鼠心肌损伤的实验研究

目的 探讨克山病病区玉米及酱块与大鼠心肌损伤的关系.方法 将雄性Wistar大鼠按体质量随机分为3组,分别饲以病区玉米、病区玉米+酱块、非病区玉米,1、3个月时检测大鼠尾静脉全血谷胱甘肽过氧化物酶(GSH-Px)活力,6个月时光镜下观察大鼠心肌病理改变.结果 1、3个月时3组大鼠全血GSH-Px活力比较,差异有统计学意义(F=23.60、72.46,P均<0.01),其中病区玉米组[(181.58±22.15)、(44.76±28.59)U/L]和病区玉米+酱块组[(195.03±17.66)、(30.38±3.35)U/L]明显低于非病区玉米组[(340.90±95.42)、(125.17±13.64)U/L,P均<0.01],而病区玉米组与病区玉米+酱块组比较,未见明显改变(P>0.05).光镜下3组大鼠心肌损伤病理改变发生率分别为3/9、1/9、2/7,组间比较差异无统计学意义(P>0.05).结论 单纯克山病病区玉米饲养可引起大鼠心肌损伤病理性改变,酱块无加重心肌损伤作用.     

克山病病区粮多不饱和脂肪酸和硒水平

克山病病区小麦亚油酸和亚麻酸含量分别为13.1±1.4g/kg 和1.05±0.13g/kg,非病区分别为10.3±2.2g/kg 和 0.71±0.19g/kg,病区明显高于非病区;病区玉米亚油酸和亚麻酸含量分别为28.1±0.22g/kg 和0.68±0.03g/kg,非病区分别为23.3±2.0g/kg 和0.57±0.07g/kg,亦病区明显高于非病区。病区小麦和玉米硒含量明显低于非病区。多不饱和脂肪酸与硒含量之间呈负相关,且均具有统计学意义。文中讨论了多不饱和脂肪酸负荷在克山病发病学中可能具有的意义。     

硒和VE对克山病病区粮及低硒人工合成饲料喂养大鼠心肌胰岛素含量的影响

饲予低硒克山病病区粮引起大鼠心肌细胞摄取胰岛素能力明显下降；在病区粮内补充一定剂量硒或维生素Ｅ可明显增加心肌细胞对胰岛素的摄取量（Ｐ＜０．０５～０．０１）；在亚硝酸钠引起的一过性缺氧条件下，单纯补硒或维生素Ｅ对心肌细胞摄取胰岛素能力无明显影响（Ｐ＞０．０５），只有联合补硒和维生素Ｅ才表现出统计学意义（Ｐ＜０．０１）；在低硒人工半合成饲料中补硒或维生素Ｅ均可显著增加心肌细胞摄取胰岛素能力（Ｐ＜０．０１）。本研究结果提示，克山病病区粮中的致病因素可原发性损害心肌细胞摄取胰岛素功能，此改变可能是病区粮引起克山病心肌损害重要环节之一。     

克山病病区玉米及酱块对大鼠心肌损伤的实验研究

Objective To investigate the myocardial damage in rats fed with corn from Keshan disease area added with bean puree. Methods Male Wistar rats were randomly divided into 3 groups according to their body weights, and fed with corn, corn from Keshan disease area added with bean puree, corn from non-endemic area. The GSH-Px activity of vena cardalis blood was examined in 1 and 3 months, rats were sacrificed after being fed for 6 months to examine the heart changes with HE stain. Results The three groups of GSH-Px activity were different in 1 and 3 months respectively(F=23.60,72.46, all P<0.01); GSH-Px activity was (181.58±22.15), (44.76±28.59)U/L in rats fed with corn, was (195.03±17.66), (30.38±3.35)U/L in those fed with corn added with bean puree from Keshan disease area, lower than the group fed with corn of non-endemic area[(340.90±95.42), (125.17±13.64)U/L, all P < 0.01]. But the difference of GSH-Px activity between simple corn group and corn adding bean puree groups of Keshan disease area was not obvious(P>0.05). Myocardial damage incidence of the three groups was 3/9,1/9,2/7. Difference among three groups did not have statistical significance(χ2=1.33, P> 0.05). Conclusions Only corn from Keshan disease area may induce myocardial damage pathology change. Adding bean puree into corn does not increase damage.     

克山病病区玉米及酱块对大鼠心肌损伤的实验研究

Objective To investigate the myocardial damage in rats fed with corn from Keshan disease area added with bean puree. Methods Male Wistar rats were randomly divided into 3 groups according to their body weights, and fed with corn, corn from Keshan disease area added with bean puree, corn from non-endemic area. The GSH-Px activity of vena cardalis blood was examined in 1 and 3 months, rats were sacrificed after being fed for 6 months to examine the heart changes with HE stain. Results The three groups of GSH-Px activity were different in 1 and 3 months respectively(F=23.60,72.46, all P<0.01); GSH-Px activity was (181.58±22.15), (44.76±28.59)U/L in rats fed with corn, was (195.03±17.66), (30.38±3.35)U/L in those fed with corn added with bean puree from Keshan disease area, lower than the group fed with corn of non-endemic area[(340.90±95.42), (125.17±13.64)U/L, all P < 0.01]. But the difference of GSH-Px activity between simple corn group and corn adding bean puree groups of Keshan disease area was not obvious(P>0.05). Myocardial damage incidence of the three groups was 3/9,1/9,2/7. Difference among three groups did not have statistical significance(χ2=1.33, P> 0.05). Conclusions Only corn from Keshan disease area may induce myocardial damage pathology change. Adding bean puree into corn does not increase damage.     

克山病病区玉米及酱块对大鼠心肌损伤的实验研究

Objective To investigate the myocardial damage in rats fed with corn from Keshan disease area added with bean puree. Methods Male Wistar rats were randomly divided into 3 groups according to their body weights, and fed with corn, corn from Keshan disease area added with bean puree, corn from non-endemic area. The GSH-Px activity of vena cardalis blood was examined in 1 and 3 months, rats were sacrificed after being fed for 6 months to examine the heart changes with HE stain. Results The three groups of GSH-Px activity were different in 1 and 3 months respectively(F=23.60,72.46, all P<0.01); GSH-Px activity was (181.58±22.15), (44.76±28.59)U/L in rats fed with corn, was (195.03±17.66), (30.38±3.35)U/L in those fed with corn added with bean puree from Keshan disease area, lower than the group fed with corn of non-endemic area[(340.90±95.42), (125.17±13.64)U/L, all P < 0.01]. But the difference of GSH-Px activity between simple corn group and corn adding bean puree groups of Keshan disease area was not obvious(P>0.05). Myocardial damage incidence of the three groups was 3/9,1/9,2/7. Difference among three groups did not have statistical significance(χ2=1.33, P> 0.05). Conclusions Only corn from Keshan disease area may induce myocardial damage pathology change. Adding bean puree into corn does not increase damage.     

克山病病区玉米及酱块对大鼠心肌损伤的实验研究

Objective To investigate the myocardial damage in rats fed with corn from Keshan disease area added with bean puree. Methods Male Wistar rats were randomly divided into 3 groups according to their body weights, and fed with corn, corn from Keshan disease area added with bean puree, corn from non-endemic area. The GSH-Px activity of vena cardalis blood was examined in 1 and 3 months, rats were sacrificed after being fed for 6 months to examine the heart changes with HE stain. Results The three groups of GSH-Px activity were different in 1 and 3 months respectively(F=23.60,72.46, all P<0.01); GSH-Px activity was (181.58±22.15), (44.76±28.59)U/L in rats fed with corn, was (195.03±17.66), (30.38±3.35)U/L in those fed with corn added with bean puree from Keshan disease area, lower than the group fed with corn of non-endemic area[(340.90±95.42), (125.17±13.64)U/L, all P < 0.01]. But the difference of GSH-Px activity between simple corn group and corn adding bean puree groups of Keshan disease area was not obvious(P>0.05). Myocardial damage incidence of the three groups was 3/9,1/9,2/7. Difference among three groups did not have statistical significance(χ2=1.33, P> 0.05). Conclusions Only corn from Keshan disease area may induce myocardial damage pathology change. Adding bean puree into corn does not increase damage.     

克山病病区玉米及酱块对大鼠心肌损伤的实验研究

Objective To investigate the myocardial damage in rats fed with corn from Keshan disease area added with bean puree. Methods Male Wistar rats were randomly divided into 3 groups according to their body weights, and fed with corn, corn from Keshan disease area added with bean puree, corn from non-endemic area. The GSH-Px activity of vena cardalis blood was examined in 1 and 3 months, rats were sacrificed after being fed for 6 months to examine the heart changes with HE stain. Results The three groups of GSH-Px activity were different in 1 and 3 months respectively(F=23.60,72.46, all P<0.01); GSH-Px activity was (181.58±22.15), (44.76±28.59)U/L in rats fed with corn, was (195.03±17.66), (30.38±3.35)U/L in those fed with corn added with bean puree from Keshan disease area, lower than the group fed with corn of non-endemic area[(340.90±95.42), (125.17±13.64)U/L, all P < 0.01]. But the difference of GSH-Px activity between simple corn group and corn adding bean puree groups of Keshan disease area was not obvious(P>0.05). Myocardial damage incidence of the three groups was 3/9,1/9,2/7. Difference among three groups did not have statistical significance(χ2=1.33, P> 0.05). Conclusions Only corn from Keshan disease area may induce myocardial damage pathology change. Adding bean puree into corn does not increase damage.