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1.
Molecular detection of bovine kobuviruses in Italy 总被引:1,自引:0,他引:1
Barbara Di Martino Federica Di Profio Elisabetta Di Felice Chiara Ceci Maria Gabriella Pistilli Fulvio Marsilio 《Archives of virology》2012,157(12):2393-2396
Faecal samples obtained from either asymptomatic or diarrhoeic calves in Italy were screened for bovine kobuviruses (BKVs) using specific primers. BKV RNA was detected in 4.9?% of the samples, with higher positivity rates in diarrhoeic calves (5.3?%) than in asymptomatic animals (4.8?%), although the difference was not statistically significant. Upon sequence analysis, all of the Italian viruses formed a tight group along with BKV-like sequences previously detected in Thailand and Japan. 相似文献
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Bawm Saw Kakisaka Keita Thu May June Chel Hla Myet Oo Yamon Min Nyunt Soe Nyein Chan Win Shwe Yee Htun Lat Lat Win Mar Mar Suzuki Hitoshi Nakao Ryo Katakura Ken 《Parasitology research》2018,117(10):3361-3364
Parasitology Research - Tick-borne intracellular protozoan parasites of the Theileria genus infect a wide range of both domestic and wild animals. In the present study, we describe the first PCR... 相似文献
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Barbara Di Martino Federica Di Profio Irene Melegari Serena Robetto Elisabetta Di Felice Riccardo Orusa Fulvio Marsilio 《Archives of virology》2014,159(7):1803-1806
Red foxes (Vulpes vulpes) are susceptible to viral diseases of domestic carnivores. In this study, by screening rectal swabs collected from 34 red foxes in Italy, we identified kobuvirus RNA in five samples. Based on analysis of partial RdRp and full-length VP1 genes, all of the strains shared the highest identity with canine kobuviruses (CaKVs) recently detected in the US, the UK and Italy. These findings provide the first evidence of the circulation of these novel viruses in foxes. 相似文献
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Mia Korva Darja Duh Ajda Puterle Tomi Trilar Tatjana Avi
upanc 《Virus research》2009,144(1-2):318-322
Different Microtus species, present in a worldwide range habitat populating North America, Europe, Asia, and few other species have been recognized previously as a hantavirus reservoir. Tula hantavirus was first reported in Microtus arvalis and Microtus rossiaemeridionalis from Central Russia and later discovered in several European countries. Using molecular techniques we have demonstrated the presence of Tula hantavirus in three different Microtus species in Slovenia. Phylogenetic analyses of partial S segment placed Slovenian strains in the same genetic lineage as Austrian and Croatian strains. 相似文献
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Díaz-Sánchez Adrian Alberto Pires Marcus Sandes Estrada Carlos Yrurzun Cañizares Ernesto Vega del Castillo Domínguez Sergio Luis Cabezas-Cruz Alejandro Rivero Evelyn Lobo da Fonseca Adivaldo Henrique Massard Carlos Luiz Corona-González Belkis 《Parasitology research》2018,117(10):3109-3118
Parasitology Research - Equine piroplasmosis is a disease of Equidae, including horses, donkeys, mules, and zebras, caused by either Theileria equi or Babesia caballi. This disease represents a... 相似文献
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The prevalence of porcine sapoviruses (SaVs) and noroviruses (NoVs) in nursing piglets on three pig farms in Ohio was studied. Fecal samples (n = 139) were collected from individual pigs and screened for caliciviruses by RT-PCR. Phylogenetic analysis was conducted using partial sequences of the RNA polymerase region. Three different SaV genogroups, including a newly emerging one (DO19 Korea-like) were detected. No NoVs were detected. Kobuviruses, emerging members of the family Picornaviridae, were detected by primers designed for SaV. To our knowledge, this is the first report of porcine DO19 Korea-like SaV and kobuvirus in the United States. 相似文献
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Joon-Yee Chung Seong-Hee Kim Yeon-Hee Kim Myoung-Heon Lee Kyoung-Ki Lee Jae-Ku Oem 《Virus genes》2013,47(3):559-562
In order to survey for feline kobuviruses infection, fecal samples (n = 39) of cats with diarrhea were collected during 2011–2012. Six (14.5 %) of the fecal samples tested were positive for feline kobuviruses. The partial nucleotide sequences of feline kobuviruses based on the RNA-dependent RNA polymerase gene were compared to those of other species. Feline kobuviruses were most closely related to canine kobuvirus in terms of their amino acid and nucleotide levels. In a phylogenetic tree, feline kobuviruses were also closely clustered with canine kobuvirus, Aichi virus (human), and mouse kobuvirus. This is the first report of the detection and genetic characterization of feline kobuviruses. 相似文献
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Alessandra Ciervo Andrea Petrucca Simonetta Ciarrocchi Antonella Pinto Lucio Bonazzi Anna Fabio Enrico Farnetti Bruno B. Chomel Lorenzo Ciceroni 《Journal of clinical microbiology》2001,39(12):4554-4557
The aim of this study was to characterize a Bartonella strain (BA-1) isolated from a blood culture of an Italian, human immunodeficiency virus-positive patient with bacillary angiomatosis. We analyzed the isolate using molecular biology methods such as whole-cell fatty acid analysis, PCR-restriction fragment length polymorphism analysis, type-specific 16S rRNA PCRs, sequence analysis of the 16S rRNA, pulsed-field gel electrophoresis, and arbitrarily primed PCR. The BA-1 isolate turned out to be a Bartonella quintana strain, similar but not identical to B. quintana Oklahoma, which was used as a control strain. 相似文献
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An outbreak of malignant ovine theileriosis among goats was confirmed and documented. In this outbreak, 16 out of 22 (72.7%)
goats died within 4 days showing clinical signs of malignant ovine theileriosis as well as in the postmortem findings. The
goats were reared in a mixed flock with sheep in Atbara Town, Northern Sudan. The infection was detected microscopically and
confirmed serologically by IFA test and molecularly by PCR technique using specific primer for Theileria lestoquardi. Hyalomma anatolicum was the most prevalent (dominant) tick species found in the farm. It is recommended to undertake future research on the role
of goats on the epidemiology of malignant ovine theileriosis. 相似文献
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Molecular detection of kobuviruses and recombinant noroviruses in cattle in continental Europe 总被引:1,自引:0,他引:1
Axel Mauroy Alexandra Scipioni Elisabeth Mathijs Christine Thys Etienne Thiry 《Archives of virology》2009,154(11):1841-1845
Two genotypes (Jena and Newbury2) and two intergenotype recombinant strains have been recognized in bovine noroviruses. Several
studies have shown an apparent predominance of bovine infection with Newbury2-related (genotype 2) strains. Bovine stool samples
were screened with two primer pairs targeting both the polymerase and the capsid genes. Among the predominant genotype 2 sequences,
two were genetically related to the recombinant strain Thirsk10. The detection of sequences genetically related to Thirsk10,
together with the very low rate of detection of Jena-related sequences, characterized the bovine norovirus population in Belgium,
a representative region of continental Europe. Unexpectedly, bovine kobuvirus-related sequences were also amplified, extending
their distribution area in Europe. 相似文献
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Two hundred and forty kennel and privately owned dogs were tested for a molecular epidemiological study on Cryptosporidium infection. Genomic DNA was extracted from individual faecal samples. All the DNA extracts were analysed with a PCR assay specific for a ∼400-bp fragment internal to the gene encoding for the Cryptosporidium oocyst wall protein. The prevalence was 3.3% and it was higher in kennel dogs and in dogs with gastrointestinal symptoms. Cryptosporidium parvum was detected by sequencing analysis in six kennel dogs and one privately owned dog, and Cryptosporidium canis was detected in one kennel dog. This is the first investigation on the prevalence of Cryptosporidium in Italian dogs and has pointed to the existence of genotypes that may be of public health significance. 相似文献
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Sasaki T Poudel SK Isawa H Hayashi T Seki N Tomita T Sawabe K Kobayashi M 《Journal of medical entomology》2006,43(1):110-112
Trench fever is a body louse-borne disease caused by Bartonella quintana Brenner. The recent status of louse infestation in Nepalese children is not well known. We collected head and body lice, Pediculus humanus capitis De Geer and Pediculus humanus humanus L., respectively, from 30 children, including 11 cases of double infestation with both head and body lice. Detection of B. quintana in both louse species identified was carried out by polymerase chain reaction (PCR). PCR products with B. quintana DNA sequences were detected in both head and body lice from two children as well as in body lice derived from two other children. These results demonstrate that head lice may also play a role in the transmission of trench fever. 相似文献
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Laura Rinaldi Maria Paola Maurelli Vincenzo Musella Antonio Bosco Helder Cortes Giuseppe Cringoli 《Parasitology research》2013,112(4):1805-1807
A cross-sectional serological survey was conducted to evaluate the prevalence of besnoitiosis in cattle farms located in a region of southern Italy. A geographical information system (GIS) was used in order to uniformly sample the bovine farms (n?=?88) throughout the entire region. Blood samples were collected from 528 autochthonous cattle and sera were tested for antibodies to Besnoitia besnoiti using an enzyme-linked immunosorbent assay test. The farm prevalence was 83.0 % (73/88), and the individual animal prevalence was 44.1 % (233/528). The availability of geo-referenced point or areal data on bovine besnoitiosis and the construction of prevalence maps by GIS are suggested for dissemination of information to veterinarians on this emerging infection in cattle. 相似文献
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M. Cinco R. Luzzati M. Mascioli R. Floris P. Brouqui 《Clinical microbiology and infection》2006,12(5):493-495
The prevalence of antibodies to Rickettsiae and other tick-borne microrganisms in the sera of 181 forestry rangers from Friuli-Venezia-Giulia, Italy, was examined. Seven (3.9%) sera were positive for Rickettsia conorii and Rickettsia helvetica, as single or dual infections; four of these sera had been found previously to be positive for Borrelia burgdorferi. Antibodies to Coxiella burnetii were detected in five (2.8%) sera, four of which were also positive for B. burgdorferi. These findings indicate that patients in this north-eastern Italian region with fever subsequent to tick-bite should be investigated for Rickettsia and Coxiella infections. 相似文献
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Dal Molin G Ansaldi F Biagi C D'Agaro P Comar M Crocè L Tiribelli C Campello C 《Journal of medical virology》2002,68(3):352-356
To assess HCV genotype distribution and its determinants, 318 consecutive HCV RNA positive patients were examined. Subtype 1b infection was the most prevalent (35.5%), followed by subtype 1a (22%), 3a (21.4%) and 2 genotype (21.3%). Subtypes 1a, 1b and 3a had a comparable prevalence (30-35%) in the 0-15-, 16-30- and 31-45-year age groups. In subjects older than 45 years, genotype 2 prevalence increased, whereas subtype 1a and 3a infections decreased markedly. In this age group types 1b and 2 accounted for a prevalence of more than 90% in a comparable proportion. Genotype prevalence rates according to different risk factors were different statistically (P < 0.001): subtype 1a and 3a infections were predominant in injection drug users (42.9% and 37.7%, respectively), whereas community acquired infections and infections in patients with a history of transfusion were caused mainly by subtype 1b (38.5% and 66.6%, respectively). Logistic regression showed that age and injection drug use are independent determinants of genotype distribution. 相似文献