O^—2对心肌细胞膜流动性,钙泵和肌酸激酶活性的影响

用恶唑烷氮氧基硬脂酸标记大鼠心肌细胞膜，通过标记膜ＥＳＲ光谱观察Ｏ＾－２发生系统对ＳＬ膜流动性，Ｃａ＾２＋泵和ＣＫ活性显著下降，均呈明显的量效关系；两个酶活性的下降与膜流动性降低密切相关；引起ＣＫ活性降低的Ｘ／ＸＯ浓度低于引起膜流动性的Ｘ／ＸＯ浓度。     

预处理对O3衰老模型超氧化物歧化酶活性的影响

为了确定预处理对小鼠Ｏ３致衰老模型是否具有保护作用,本研究利用ＳＯＤ微量快速测定方法,检测和分析了经云南花粉田七口服液预处理后小怀血和心肌中超氧化物歧化酶活性,结果显示：,预处理实验组ＳＯＤ活性明显高于空白姐和阳性对照组,同时,空白姐与阳性对照组之间无显著性差异。这表明：ＹＮ－ＰＴＯＬ预处理改善或提高Ｏ３致老小展开机体内ＳＯＤ活性,是一种有应用前景的延缓衰老的保健药物。     

Graves病患者血清SOD活性改变及意义

含铜、锌离子的超氧化物歧化酶(C·Zn-Superoxide dismutast,Cu·Zn-SOD),是机体清除超氧阴离子自由基(O_2~-)的一种重要的酶系统。近年来研究表明,机体多种疾病的病理、生理过程与SOD紧密相关。本文以Graves病患者为研究对象,测定血清Cu·Zn-SOD,以期探讨二者之间的关系。     

人参固本口服液的药理实验研究

人参固本口服液系人参固本丸改变剂型 ,具有吸收快 ,便于服用之优点。为验证其药理药效 ,对新老两种剂型进行了主要药效学对比实验 ,结果显示 :人参固本丸及口服液对小鼠均有明显的抗疲劳、耐缺氧作用 ,可提高小鼠腹腔内巨噬细胞的吞噬功能 ;对阴虚模型动物滋阴补肾及抗氧化作用实验表明 :二者对阴虚小鼠的甲状腺、胸腺、肾上腺均有增加趋势 ,尤其胸腺明显增大 ,说明二者均有滋阴补肾 ,改善因肾虚所致内分泌腺体萎缩 ,使失调的功能恢复至生理平衡状态 ,提高机体免疫功能的作用 ,并可使小鼠血液及胸腺组织中SOD活力显著提高 ,使血浆及脑组织中MDA含量明显下降 ,说明二者均能起到较强的清除自由基和抗脂质过氧化作用     

芒柄花黄素对超氧阴离子及羟自由基清除作用的研究

目的:探讨芒柄花黄素体外对氧自由基的抑制和清除作用。方法:采用分光光度法检测芒柄花黄素对超氧阴离子自由基(O2-)和羟自由基(.OH)的清除及抑制作用。结果:芒柄花黄素终浓度为10、20、40μg.ml-1时,在体外对O2-的清除率分别为42.19%,56.64%及65.43%,对O2-生成的抑制率分别为31.06%,44.72%及63.35%;而对.OH的清除率分别为34.98%,41.06%及61.60%。结论:芒柄花黄素对O2-具有明显的抑制及清除作用,其对.OH也有明显的清除作用。     

超氧阴离子自由基对大鼠肝卵圆细胞胞内自由钙浓度的影响

采用激光共聚焦扫描显微镜,观察黄嘌呤黄嘌呤氧化酶反应系统（Ｘ／ＸＯ）生成不同浓度的超氧阴离子自由基（Ｏ２）致培养的大鼠肝卵圆细胞（ＷＢ细胞）胞质内钙浓度的变化,结果发现：仅小剂量的Ｏ２引起胞质内钙浓度升高,约３０Ｓ后达到峰值,６０ｓ后恢复正常。部分细胞观察到数次钙浓度间断升高、幅度逐渐下降的现象。超氧化物歧化酶（ＳＯＤ）可抑制此变化,过氧化氢酶（ＣＡＴ）无效果,细胞在无钙液中观察仍出现钙峰,但受     

旋磁场对小白鼠血清超氧化物岐化酶影响的实验研究

本文报道了用化学发光法测定经平均磁场强度为0.09T,转速为2000r/min的旋磁场处理后小白鼠血清中超氧化物岐化酶的含量。结果证明:经旋磁场处理后,小白鼠血清中超氧化物岐化酶的含量明显高于对照组(P<0.001)。可见,一定强度的旋磁场作用于小白鼠能提高其机体抗氧化酶水平。     

自由基在肾缺血再灌流损伤中作用的实验研究

本实验选用大鼠右肾切除、左侧肾蒂夹闭60min肾缺血模型,观察缺血、再灌流、再灌流加别嘌呤醇(AP)和再灌流加超氧化物歧化酶(SOD)对动物血清肌酐(Scr)、肾系数和肾形态学变化的影响。结果发现60min肾缺血后再灌流24hr,动物的Scr水平明显高于单纯缺血组动物的水平(P<0.01)和假手术组动物的水平(P<0.01);光镜下肾组织损伤较单纯缺血组动物明显加重,表明再灌流可以加重缺血肾损伤,动物发生缺血性急性肾衰(IARF)。给AP和SOD处理后,肾衰动物的Scr水平、肾系数分别较单纯再灌流组动物明显降低(P<0.01)。整个肾组织病变尤其是线粒体病变、刷状缘损伤大为减轻。说明AP和SOD可以减轻IARF,自由基可能参于肾缺血再灌流损伤,膜和线粒体损伤可能是IARF发病的重要因素。     

维生素C对应激大鼠脑内超氧化物歧化酶含量的影响

目的 :探讨在急性应激状态下大鼠脑内超氧化物歧化酶 (SOD)活性的变化及维生素C (VitC)对SOD改变的影响。方法 :在强迫游泳应激模型中 ,大鼠用VitC慢性给药 (腹腔内注射 0 5g·Kg- 1 ,每日一次 ,连续 7天 ) ,观察强迫游泳应激后大鼠额叶、海马、中脑和下丘脑内SOD的活性。结果 :与对照组比较 ,强迫游泳组大鼠海马内SOD活性显著性增高 (t=3 3 2 4,P <0 0 1) ,额叶、中脑和下丘脑SOD活性也显著升高 (t =2 5 3 3 ,2 3 0 3 ,2 5 89,P均 <0 0 5 )。VitC组与对照组各脑区SOD活性未见明显变化 (P均 >0 0 5 ) ;但与强迫游泳组比较 ,海马内SOD活性显著性降低 (t=3 812 ,P <0 0 1) ,额叶、中脑和下丘脑内SOD活性也显著性降低 (t=2 2 5 1,2 3 42 ,2 2 16,P均 <0 0 5 )。结论 :VitC可显著减少急性应激状态下脑内产生的自由基 ,具有神经保护作用。     

恒定磁场对小鼠肝组织超氧化物歧化酶活力及过氧化化脂质水平的影响

将小鼠随机分成实验组Ａ，Ｂ及对照组共三组。实验组Ａ经８０ｍＴ磁场场处理；实验组Ｂ经６０ｍＴ磁场正理２，每日３０分钟。     

An iron-containing superoxide dismutase in Tritrichomonas foetus

Dialysed extracts of Tritrichomonas foetus were found to have superoxide dismutase at substantially higher levels than those found in trypanosomatids and mouse red blood cells. The activity was sensitive to inhibition by H₂O₂ but not by cyanide, suggesting that this organism has iron-containing superoxide dismutase(s). Three isozymes were seen by isoelectric focusing which appeared to be sensitive to inhibition by H₂O₂.     

Protective role of superoxide dismutase in human sperm motility: superoxide dismutase activity and lipid peroxide in human seminal plasma and spermatozoa.

The levels of superoxide dismutase (SOD), a highly specific scavenging enzyme for superoxide anion radicals (O2-), and lipid peroxide produced by oxygen free radicals were measured in human seminal plasma and spermatozoa. Seminal plasma contained 366.8 +/- 20.9 U/ml (mean +/- SE) of SOD activity. SOD activity in human spermatozoa showed a significant correlation to the number of motile spermatozoa, while the activity in seminal plasma did not relate to the sperm concentration or motility. The lipid peroxide concentration in seminal plasma was 6.22 +/- 0.46 nmol/ml and had no significant relationship to sperm concentration or motility. The malondialdehyde (MDA) concentration in spermatozoa was significantly related to the number of immotile spermatozoa. A decrease in the motility of spermatozoa incubated in medium without seminal plasma was observed after 120 min, while the MDA concentration of the spermatozoa increased. Addition of exogenous SOD (400 U/ml) to the sperm suspension significantly decreased this loss of motility and the increase of the MDA concentration. These data suggest a significant role for SOD in sperm motility. It seems that lipid peroxidation of human spermatozoa may cause loss of motility and that SOD may inhibit this lipid peroxidation. These results suggest that SOD may have a possible clinical application in the use of spermatozoa for in-vitro fertilization (IVF) or artificial insemination.     

Activity and electrophoretic multiplicity of molecular forms of superoxide dismutase in human blood cells

The level of superoxide dismutase (SOD) activity calculated relative to protein in the various human blood cells falls in the following order: platelet > erythrocyte > lymphocyte > granulocyte. During electrophoresis in homogeneous polyacrylamide gel of homogenates of granulocytes, lymphocytes, and platelets three zones of SOD activity were identified. Two fractions of the enzyme, disappearing after treatment with cyanide, were found in lysates of erythrocytes after removal of the hemoglobin. Of the two SOD fractions of platelets, lymphocytes, and granulocytes migrating rapidly toward the anode, the first corresponds in its ability to be inhibited by cyanide or organic solvents to the cytosol isozyme, the second to the mitochondrial isozyme. The third cathode fraction was not identified. The functional role of SOD in specialized blood cells and the cause of heterogeneity of the enzyme are discussed.Department of Hyperbaric Oxygenation, All-Union Scientific-Research Institute of Clinical and Experimental Surgery, Moscow. Department of Biochemistry, Medico-Biological Faculty, N. I. Pirogov Second Moscow Medical Institute. (Presented by Academician S. E. Severin.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 84, No. 8, pp. 166–168, August, 1977.     

Different mechanisms for the induction of copper-zinc superoxide dismutase and manganese superoxide dismutase by progesterone in human endometrial stromal cells

BACKGROUND: The present study was undertaken to investigate the cAMP-dependent regulation of copper-zinc superoxide dismutase (Cu,Zn-SOD) and manganese SOD (Mn-SOD) by ovarian steroids in human endometrial stromal cells (ESC). METHODS and RESULTS: To examine the effect of cAMP on SOD expression, ESC were incubated with dibutyryl-cAMP (db-cAMP, 0.5 mmol/l), forskolin (25 micromol/l), or estradiol (E(2), 10(-8) mol/l) + medroxyprogesterone acetate (MPA, 10(-6) mol/l), for 18 days. E(2) + MPA significantly increased Cu,Zn-SOD activity and mRNA concentrations, whereas db-cAMP and forskolin had no effect. On the other hand, Mn-SOD activity and mRNA concentration were significantly increased by all of these treatments. Insulin-like growth factor-binding protein-1, a marker of decidualization, was clearly induced by db-cAMP, forskolin or E(2) + MPA, accompanied by morphological changes characteristic of decidualization. To study whether the increase in Mn-SOD by db-cAMP or E(2) + MPA was mediated by cAMP-dependent protein kinase A (PKA), ESC were incubated with protein kinase inhibitor (PKI) (10 microg/ml), an inhibitor of PKA, in the presence of db-cAMP or E(2) + MPA. The increase in Mn-SOD activity following db-cAMP or E(2) + MPA was completely inhibited by PKI. CONCLUSIONS: In the process of decidualization, E(2) + MPA increases Mn-SOD expression via a cAMP-dependent pathway. Cu,Zn-SOD is also up-regulated by E(2) + MPA, but via a different pathway from that involving cAMP.     

The suppressive effect of gelatin-conjugated superoxide dismutase on disease development and severity of collagen-induced arthritis in mice.

We studied the effect of superoxide dismutase (SOD) on murine collagen-induced arthritis (CIA), an animal model of human rheumatoid arthritis (RA). Among SOD derivatives studied, only gelatin-SOD conjugate which has prolonged half life in vivo was effective to suppress the development of CIA, while native SOD or gelatin carrier alone was ineffective. Interestingly, pyran polymer-conjugated SOD which also has a long half life showed no suppressive effect on the disease. No significant effect on immune response against type II collagen (CII) was found in any of the experimental groups. In addition, induction of suppressor cells was not detected in spleen or lymph node cells of the gelatin-SOD-treated group. Therefore, these results suggest that oxygen radicals may have an important role in the effector phase of the immune response to manifest this chronic autoimmune polyarthritis. Thus, the use of appropriate antioxidants for the treatment of human RA may be rationalized.     

Changes in superoxide dismutase activity during stimulation of peripheral blood polymorphonuclear leukocytes

No. 4 Department of Biophysics and Internal Medicine, N. I. Pirogov Second Moscow Medical Institute. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 109, No. 4, pp. 334–336, April, 1990.     

溃疡性结肠炎患者血清超氧化物歧化酶及脂质过氧化物的变化

用黄嘌呤氧化法及硫代巴比妥酸反应产物比色法分别对120例溃疡性结肠炎患者血清超氧化物歧化酶（SOD）及脂质过氧化物（LPO）含量进行检测。结果表明：溃疡性结肠炎患者SOD明显低于对照组（P＜0．001）;LPO明显高于对照组（P＜0．001）。其中单纯湿热内蕴型或兼胃虚弱型或兼脾虚气滞血瘀型或兼阴虚气滞血瘀型血清SOD含量均明显低于对照组（P＜0．001）;而血清LPO含量均明显高于对照组（P＜0．001）。结果提示：以湿热内蕴型为主的溃疡性结肠炎患者清除氧自由基,保护细胞免受损伤的SOD降低,而间接反映细胞损伤的LPO升高。当伴有虚证时,尤以脾胃虚弱型或脾虚气滞血瘀型的溃疡性结肠炎患者SOD含量明显降低和LPO含量明显增高。     

预处理对O_3衰老模型超氧化物歧化酶活性的影响

为了确定预处理对小鼠 O3致衰老模型是否具有保护作用 ,本研究利用 SOD微量快速测定法 ,检测和分析了经云南花粉田七口服液 (Yun Nan Pollen Tienchi Oral L iquid,简称 YN- PTOL )预处理后小鼠全血和心肌中超氧化物歧化酶 (superoxide dismutase,SOD)的活性 ,结果显示 :预处理实验组 SOD活性明显高于空白对照组和阳性对照组 (P<0 .0 5 ) ,同时 ,空白对照组与阳性对照组之间无显著性差异。这表明 :YN- PTOL预处理能改善或提高 O3致老小鼠模型机体内 SOD活性 ,是一种有应用前景的延缓衰老的保健药物。     

Protective action of superoxide dismutase in experimental myocarditis

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