SERUM AMYLOID P COMPONENT NOT ASSOCIATED WITH C4B一BINDING PROTEIN IN HUMAN SERUM SHOWN BY CROSSED IMMUNOELECTROPHORESIS IN TH

ＳＥＲＵＭＡＭＹＬＯＩＤＰＣＯＭＰＯＮＥＮＴＮＯＴＡＳＳＯＣＩＡＴＥＤＷＩＴＨＣ４Ｂ一ＢＩＮＤＩＮＧＰＲＯＴＥＩＮＩＮＨＵＭＡＮＳＥＲＵＭＳＨＯＷＮＢＹＣＲＯＳＳＥＤＩＭＭＵＮＯＥＬＥＣＴＲＯＰＨＯＲＥＳＩＳＩＮＴＨＥＰＲＥＳＥＮＣＥＯＦＣＡＬＣＩＵ...     

EFFECT OF EXCITATORY AMINO ACIDS．Ca~（2＋） ON DELAYED NEURONAL DEATH IN HIPPOCAMPUS FOLLOWING TRANSIENT FOREBRAINISCHEMIA IN GE

ＥＦＦＥＣＴＯＦＥＸＣＩＴＡＴＯＲＹＡＭＩＮＯＡＣＩＤＳ．Ｃａ~（２＋）ＯＮＤＥＬＡＹＥＤＮＥＵＲＯＮＡＬＤＥＡＴＨＩＮＨＩＰＰＯＣＡＭＰＵＳＦＯＬＬＯＷＩＮＧＴＲＡＮＳＩＥＮＴＦＯＲＥＢＲＡＩＮＩＳＣＨＥＭＩＡＩＮＧＥＲＢＩＬＳ￥ＸｕＣｈａｎｇｑｉ?..     

IMPROVEMENT OF A DIRECT DTNB ASSAY OF GLUTATHIONE PEROXIDASE AND EFFECT OF SELENIUM RICH YEAST IN MICE BLOOD GLUTATHIONE PERO

ＩＭＰＲＯＶＥＭＥＮＴＯＦＡＤＩＲＥＣＴＤＴＮＢＡＳＳＡＹＯＦＧＬＵＴＡＴＨＩＯＮＥＰＥＲＯＸＩＤＡＳＥＡＮＤＥＦＦＥＣＴＯＦＳＥＬＥＮＩＵＭＲＩＣＨ　ＹＥＡＳＴＩＮＭＩＣＥＢＬＯＯＤＧＬＵＴＡＴＨＩＯＮＥＰＥＲＯＸＩＤＡＳＥＡＣＴＩＶＩＴＹＢａｏＪ...     

EFFECTS OF SYSTEMIC FLUCONAZOLE THERAPY ON IN VITRO ADHESION OF CANDIDA ALBICANS TO BUCCAL EPITHELIAL CELLS AND CHANGES OF TH

ＥＦＦＥＣＴＳＯＦＳＹＳＴＥＭＩＣＦＬＵＣＯＮＡＺＯＬＥＴＨＥＲＡＰＹＯＮＩＮＶＩＴＲＯＡＤＨＥＳＩＯＮＯＦＣＡＮＤＩＤＡＡＬＢＩＣＡＮＳＴＯＢＵＣＣＡＬＥＰＩＴＨＥＬＩＡＬＣＥＬＬＳＡＮＤＣＨＡＮＧＥＳＯＦＴＨＥＣＥＬＬＳＵＲＦＡＣＥＰＲＯＴＥＩＮ...     

EFFECTS OF MTX AND BN_52021 ON PAF－INDUCED CHEMOTAXIS OF PMNs AND I

ＥＦＦＥＣＴＳＯＦＭＴＸＡＮＤＢＮ５２０２１ＯＮＰＡＦ－ＩＮＤＵＣＥＤＣＨＥＭＯＴＡＸＩＳＯＦＰＭＮｓＡＮＤＩＮＴＲＡＥＰＩＤＥＲＭＡＬＡＣＣＵＭＵＬＡＴＩＯＮＯＦＩＮＦＬＡＭＭＡＴＯＲＹＣＥＬＬＳＩＮＧＵＩＮＥＡＰＩＧＳＬｉｕＢａｏｊｕｎ刘宝军，Ｚ...     

Gallium-67 scanning for detection of alveolitis in idiopathic pulmonary fibrosis and sarcoidosis.

ＧＡＬＬＩＵＭ－６７ＳＣＡＮＮＩＮＧＦＯＲＤＥＴＥＣＴＩＯＮＯＦＡＬＶＥＯＬＩＴＩＳＩＮＩＤＩＯＰＡＴＨＩＣＰＵＬＭＯＮＡＲＹＦＩＢＲＯＳＩＳＡＮＤＳＡＲＣＯＩＤＯＳＩＳＪｉｎＳｈｅｎｇ金生，ＷａｎｇＧｕａｎｇｆａ王广发，ＨｅＢｉｎｇ何冰ａｎｄＺｈｕ...     

INTEGRATION OF TRADITIONAL AND MODERN METHODS IN THE IDENTIFICATION OF AFB CULTURES ISOLATED FROM CLINICAL SPECIMENS OF PATIE

ＩＮＴＥＧＲＡＴＩＯＮＯＦＴＲＡＤＩＴＩＯＮＡＬＡＮＤＭＯＤＥＲＮＭＥＴＨＯＤＳＩＮＴＨＥＩＤＥＮＴＩＦＩＣＡＴＩＯＮＯＦＡＦＢＣＵＬＴＵＲＥＳＩＳＯＬＡＴＥＤＦＲＯＭＣＬＩＮＩＣＡＬＳＰＥＣＩＭＥＮＳＯＦＰＡＴＩＥＮＴＳＷＩＴＨＳＫＩＮＤＩＳＥＡＳ...     

IMPLANTATION OF AUTOLOGOUS SKIN FIBROBLAST GENETICALLY MODIFIED TO SECRETE CLOTTING FACTOR IX PARTIALLY CORRECTS THE HEMORRHA

ＩＭＰＬＡＮＴＡＴＩＯＮＯＦＡＵＴＯＬＯＧＯＵＳＳＫＩＮＦＩＢＲＯＢＬＡＳＴＧＥＮＥＴＩＣＡＬＬＹＭＯＤＩＦＩＥＤＴＯＳＥＣＲＥＴＥＣＬＯＴＴＩＮＧＦＡＣＴＯＲＩＸＰＡＲＴＩＡＬＬＹＣＯＲＲＥＣＴＳＴＨＥＨＥＭＯＲＲＨＡＧＩＣＴＥＮＤＥＮＣＩＥＳＩＮ...     

CHANGES OF MONOAMINES，PURINES AND AMINO ACIDS IN RAT STRIATUM AS MEASURED BY INTERCEREBRAL MICRODIALYSIS DURING ISCHEMIA／REPE

ＣＨＡＮＧＥＳＯＦＭＯＮＯＡＭＩＮＥＳ，ＰＵＲＩＮＥＳＡＮＤＡＭＩＮＯＡＣＩＤＳＩＮＲＡＴＳＴＲＩＡＴＵＭＡＳＭＥＡＳＵＲＥＤＢＹＩＮＴＥＲＣＥＲＥＢＲＡＬＭＩＣＲＯＤＩＡＬＹＳＩＳＤＵＲＩＮＧＩＳＣＨＥＭＩＡ／ＲＥＰＥＲＦＵＳＩＯＮ￥ＺｈａｎｇＪｉ...     

SURGICAL TREATMENT OF BONE TUMORS IN CONJUNCTION WITH MICROWAVE－INDUCED HYPERTHERMIA AND ADJUVANT IMMUNOTHERAPY A PRELIMINARY

ＳＵＲＧＩＣＡＬＴＲＥＡＴＭＥＮＴＯＦＢＯＮＥＴＵＭＯＲＳＩＮＣＯＮＪＵＮＣＴＩＯＮＷＩＴＨＭＩＣＲＯＷＡＶＥ－ＩＮＤＵＣＥＤＨＹＰＥＲＴＨＥＲＭＩＡＡＮＤＡＤＪＵＶＡＮＴＩＭＭＵＮＯＴＨＥＲＡＰＹＡＰＲＥＬＩＭＩＮＡＲＹＲＥＰＯＲＴＦａｎＱｉｎｇｙ...     

肝癌和癌旁组织中HCV—RNA及其负链的研究

该研究采用ELISA法,对肝癌患者的血清检测HCV—Ab,用Nested-PCR法对患者的血清、肝癌组织和癌旁组织检测HCV-RNA和HCV-RNA负链,探讨肝细胞癌中是否存在HCV-RNA及其复制状态。对照组血清检测HCV-Ab、HCV-RNA1/17阳性。肝癌组16/31 HCV-RNA阳性;癌旁组织15例测到HCV-RNA负链,癌组织只有6例测到负链。结论:血清中抗HCV检测不能完全反映肝内是否曾有HCV感染情况。癌组织和癌旁组织均能检出HCV-RNA,说明肝癌组织中感染着丙型肝炎病毒。肝癌组织中确实存在HCV-RNA负链,说明HCV-RNA能在肝组织内不断地自我复制。     

Usefulness of complement split product, Bb, as a clinical marker for disease activity of lupus nephritis

To evaluate the usefulness of Bb, a split product of complement factor B, as a clinical marker for disease activity of lupus nephritis, we measured the Bb concentration of sera from 42 patients with lupus nephritis. Serum Bb levels were significantly higher in patients with active nephritis (active nephritis group, n= 30) than in patients with nephritis in remission (remission group, n=12) (14.3+/-8.3 versus 7.4+/-5.9 microg/ml; p = 0.012). In contrast, there was no significant difference in serum C3 levels between active nephritis group and remission group (42.5+/-20.9 versus 44.7+/-15.9 mg/dl ; p = 0.77). In the comparison of Bb levels between active nephritis group and remission group, the sensitivity was 66.6%, specificity was 83.3%, and the positive and negative likelihood ratios were 3.95% and 0.41%, respectively. The present results suggest that serum Bb level is a useful clinical marker for disease activity in lupus nephritis.     

Detection of herpes simplex virus type 1 in rheumatic valvular tissue

Background Rheumatic heart disease (RHD) is the most important sequela of rheumatic fever (RF):evidence that streptococcal infection is aetiological is prominent, but sometimes contradictory. Acute HSV-1 infection in mouse leads to carditis and valvulitis whereas recurrent infection results in inflammatory granulomatous lesions that resemble Aschoff bodies. Cells containing HSV-1 inclusions or virus infected giant cells appear similar to Anitschkow cells or Asehoff cells respectively. We hypothesized that HSV-1 infection also may be involved in RHD.Methods Formalin-fixed, paraMn-embedded valvular tissue samples from 32 patients with RHD were investigated for evidence of HSV-1 infection. HSV-1 antigen was detected by immunohistoehemistry, using HSV-1-specific monoclonal and polyclonal antibodies. HSV-1 glyeoprotein D gene sequences were amplified by nPCR,using β-globin gene amplification in the same samples as internal control. Valvular tissue from 5 cases of sudden death and 3 cases died of neisseria meningitis without a history of valvular disease was used for comparison. HSV-1-infected lung tissue was used as positive control.Results HSV-I antigens were detected in valvular tissues from 21 of 32 (65.6%) patients. Fifteen of these 21 (46.9% of cases), but no antigen-negative sample, were positive also for HSV DNA. Nueleotide sequence of PCR products was homologous to the targeted region of the HSV-1 glycoprotein D gene. HSV-1 antigen was present also in one case of sudden death but viral DNA was not found in any tissue sample from tile comparison group. Results from reagent and positive controls were as anticipated.Conclusions This is the first study to show the presence of HSV-1 antigen and genomic DNA in valvular tissues from patients with RHD and provides evidence for an association of HSV-1 infection with some cases of rheumatic valvular disease.     

获得性大疱性表皮松解症的鉴定

[目的]对经过临床表现、病理、直接免疫荧光等常规手段诊断为大疱性类天疱疮(BP)的患者血清进行回顾性研究,以确定获得性大疱性表皮松解症(EBA)在该组人群中的发生率,并比较常规间接免疫荧光(IIF)技术和盐裂皮肤IIF技术在检测BP患者血清中抗基底膜带(BMZ)抗体的敏感性。[方法]分别应用常规IIF技术和盐裂皮肤IIF技术对37例BP患者及10例正常对照血清中抗BMZ抗体进行检测。[结果]常规IIF示37例BP患者中23例血清抗BMZ抗体阳性,阳性率为62.16%,10例正常对照血清阴性;盐裂皮肤IIF示37例BP患者中35例血清抗BMZ抗体阳性,阳性率为94.59%,其中31例为表皮侧IgG沉积、1例为真皮侧沉积、3例为双侧沉积,10例正常对照血清阴性。盐裂皮肤IIF检测BP患者血清中抗BMZ抗体的阳性率与常规IIF相比明显升高,具有显著性差异(P=0.001<0.01)。[结论]37例BP患者中检出1例EBA(盐裂皮肤IIF示真皮侧沉积),EBA在经过常规方法诊断为BP的患者中的发生率为2.7%(1/37),检测BP患者血清抗BMZ抗体时盐裂皮肤IIF技术比常规IIF技术敏感。     

BORRELIA BURGDORFERI DNA IN BIOLOGICAL SAMPLES FROM PATIENTS WITH SARCOIDOSIS USING THE POLYMERASE CHAIN REACTION TECHNIQUE

Polymerase chain reaction (PCR) was used to detect the presence of Borretia burgdoferi DNA in biological samples from patients with sarcoidcsis. The target DNA sequence was of chromosomal origin. The amplified DNA sequence was analyzed by agarose gel electrophoresis, PAGE with silver staining, and the identity of amplified DNA was confirmed by restriction enzyme cleavage and DNA-DNA hybridlzation with a ^32P-labelled probe. The assay was sensitive to fewer than two copies of B. burgdor feri genome, even in the presence of a 10^4-fold excess of human eukaryotic DNA, and was also specific to different B. burgdorferl strains tested. Sera seroiogieally positive to B. burgdorferi (n=26), broncbemlveolar lavage fluid and supematant of BALF (n=26) and peripheral blood (n=9) from sarcoidosis patients were tested. The positive rate was low (4/26, 2/26, and 0/9, respectively). It was considered that DNA from B. bur gdor feri may be identified in a minority of patients with s,arcoidosis, and it may play a pathogenetic rote in such cases. More studies need to be done before advancing the hypothesis of an etiologic role of B. burgdorferi in sarcoidosis.     

胆结石患者胆汁幽门螺杆菌免疫印迹检测分析

目的 研究幽门螺杆菌感染与胆石形成的关系。方法 采用酶链免疫法(ELASA)对胆结石患者血清及胆汁免疫球蛋白IgG、IgA、IgM标本进行检测，对血清学及胆汁IgG均阳性患者的胆汁，采用免疫印迹法(Western Blot)进行幽门螺杆菌感染相关蛋白检测。结果 50例患者血清学阳性35例(70．0％)，胆汁阳性29例(58．0％)。血清学及胆汁均阳性24例(480％)。血清学及胆汁均阳性24例中，Cag A检出17例(70．8％)，Vac A检出11例(45．8％)。Ure B及Ure A分别为18例(75．0％)和16例(66.7％)。同时检出幽门螺杆菌细胞空泡毒素(Vac A)相关亚单位37kD、35kD糖蛋白。结论 胆汁中存在多种幽门螺杆菌感染相关蛋白，并可能参与胆结石的形成。     

原发性肝癌血清雌二醇含量与肝组织雌二醇受体的初步研究

应用放免法测定肝癌组,肝硬化组及正常对照组血清雌二醇的含量,并对10例肝癌,18例肝硬化进行了雌二醇受体的组织化学检查。结果表明;雌二醇受体主要分布于肝癌的癌细胞浆内,受体阳性例数较肝硬化例明显减少,着色强度明显减弱。肝癌患者血清雌二醇含量明显低于肝硬化及正常对照组。肝癌血清雌二醇含量与组织内雌二醇受体阳性细胞百分比无平行关系。认为不能以血清雌二醇含量估计组织内相应受体的多少。     

实时荧光定量PCR在结节病与不典型结核鉴别诊断中的临床应用

目的验证和评价实时荧光定量PCR（polymerase chain reaction）的方法检测结核分枝杆菌DNA在结节病与不典型结核病鉴别诊断中的实际应用价值。方法以2008年6月至2009年6月期间,经病理活检证实为肉芽肿性疾病,但无法确定为结节病或结核病的49例患者为研究对象。运用我科前期建立的实时荧光定量PCR的方法检测上述患者石蜡包埋病理组织标本中的结核分枝杆菌DNA（Tuberculosis polymerase chain reaction,TBPCR）,以1.14×10~3拷贝/ml作为鉴别结节病与不典型结核病的最佳cutoff值,结合临床、影像、病理和其他检查结果帮助临床鉴别诊断并指导用药。随访所有患者至2009年8月1日,通过临床表现、影像学、治疗效果等变化来验证和评价本方法的实际应用价值。结果 49例患者定量TB-PCR结果中,10例患者结核分枝杆菌DNA为阳性（2.01×10~3～7.98×10~4拷贝/ml）（阳性率20.41%）,39例为阴性。结合患者临床资料及TB-PCR结果确定诊断,33例为结节病,2例结核病,诊断明确率达到72%（35/49）。诊断明确患者治疗后随访（2～14个月）,33例结节病和2例结核病患者经相应治疗均达到稳定或不同程度好转,无1例出现病情恶化。结论实时定量PCR法检测石蜡包埋组织中结核菌DNA能灵敏而高效地检出不典型结核病肉芽肿中的结核杆菌DNA,可作为鉴别结节病和不典型结核病的有效方法之一。     

HLA-A and HLA-B antibodies of pregnant mothers in Malaysia

The aim of this study was to determine the frequency and specificity of HLA-A and B antibodies in multiparous mothers in the Malaysian population. 1,100 maternal serum samples obtained during normal childbirth were screened against a panel of 100 lymphocytes with known HLA antigen types for HLA antibodies by the complement dependent lymphocyte microcytotoxicity dye exclusion test. From the total number of 1,100 samples of maternal serum that were screened for HLA antibodies only 205 specimens (18.6%) tested positive for antibodies. The percentage of maternal sera which contained HLA-B specificities (10.6%) were significantly higher than those which contained HLA-A specificities (3.0%). Sixty maternal serum samples (5.5%) had high enough titres to be utilised as tissue typing reagents. Thirty nine maternal serum samples (3.5%) contained monospecific HLA antibodies. In this study the most common monospecific HLA antibodies characterised included the following specificities: A2, B5, B17 and B40. Malaysian multiparous mothers of gravida 3, 4 and 5 had a higher frequency for producing HLA-antibodies.     

用合成肽制备乙型肝炎病毒前S_2抗体及其诊断应用

根据乙型肝炎病毒(HBV)前S_2抗原(Pre S_2)氨基酸顺序合成了P120-145多肽,制备抗血清,用于检测乙肝患者血清中Pre S_2,并研究了与HBV DNA及其他HBV标志物的相关性。结果表明:HBeAg阳性77例,Pre S_2阳性74例(96.1％)。抗HBe IgM及PHSA-r阳性23例,血清Pre S_2阳性19例(82.6％)。HBV DNA阳性26例,血清Pre S_2阳性25例(96.2％)。说明血清Pre S_2阳性可以反映HBV复制。