16例足跟部软组织缺损的皮瓣修复

目的探讨足跟部软组织缺损、跟腱外露的修复方法。方法2002年1月至2005年2月按缺损面积大小应用腓肠神经经营皮瓣及矩形推进皮瓣修复足跟部软组织缺损、跟腱外露16例，观察皮瓣成活率、感觉、外观等情况。结果16例患者15例皮瓣一期成活，创面顺利愈合，1例矩形推进皮瓣创缘坏死，创面裂开，经腓肠神经营养皮瓣修复后治愈。皮瓣感觉良好，4例腓肠神经营养皮瓣稍显臃肿，跟腱无粘连，踝关节功能良好。结论对缺损面积4．0cm×5．0cm以内者可应用局部顺利推进皮瓣修复，缺损面积4．0cm×5．0cm以上者宜用腓肠神经营养皮瓣修复。     

腓肠神经营养血管蒂逆行岛状筋膜皮瓣的临床应用

目的探讨腓肠神经营养血管蒂逆行岛状筋膜皮瓣的临床应用范围及效果。方法2002年3月～2006年11月对13例小腿下1/3段及足踝部软组织缺损的病例切取腓肠神经营养血管蒂逆行岛状筋膜皮瓣进行创面转移修复,皮瓣面积最大15cm×10cm,最小6cm×5cm。结果13例中11例皮瓣全部存活,2例远端皮缘缺血坏死,经局部换药及游离植皮后创面愈合。随访12例,失访1例,随访时间6￣18个月,软组织缺损创面修复满意且外形良好。结论该皮瓣的神经营养血管血供可靠,术中切取简单易行且安全,成功率高,是修复小腿下1/3段、足踝部软组织缺损的理想皮瓣。     

腓肠神经营养血管蒂逆行岛状筋膜皮瓣的临床应用

目的 探讨腓肠神经营养血管蒂逆行岛状筋膜皮瓣的临床应用范围及效果.方法 2002年3月～2006年11月对13例小腿下1/3段及足踝部软组织缺损的病例切取腓肠神经营养血管蒂逆行岛状筋膜皮瓣进行创面转移修复,皮瓣面积最大15cm×10cm,最小6cm×5cm.结果 13例中11例皮瓣全部存活,2例远端皮缘缺血坏死,经局部换药及游离植皮后创面愈合.随访12例,失访1例,随访时间6～18 个月,软组织缺损创面修复满意且外形良好.结论 该皮瓣的神经营养血管血供可靠,术中切取简单易行且安全,成功率高,是修复小腿下1/3段、足踝部软组织缺损的理想皮瓣.     

内踝上皮支皮瓣的临床应用

目的 :探讨内踝上皮支皮瓣的临床应用价值。方法 :设计应用内踝上皮支皮瓣 6例。皮肤及软组织缺损部位 :同侧踝部 2例 ,同侧足跟部 2例 ,对侧足背部 1例 ,对侧内踝部 1例。皮肤及软组织缺损面积 :3cm× 4cm～ 7cm× 1 0 cm。切取内踝上皮支皮瓣面积 :4cm× 7cm～ 8cm× 1 7cm。结果 :皮瓣全部成活 5例 ,皮瓣远端皮肤部分坏死 1例 ,经换药痂下愈合。结论 :内踝上皮支皮瓣局部转移操作简单 ,成活率高 ,是修复同侧或对侧下段、踝部及足跟部的皮肤软组织缺损的良好方法     

颈肩胸超薄皮瓣修复颈前瘢痕的体会(附9例报告)

1994年以来 ,我院应用颈肩胸超薄皮瓣修复颈前区瘢痕孪缩畸形共 9例 ,获得较好效果 ,报告如下。1　临床资料1.1　一般资料　本组男 6例 ,女 3例 ,年龄 3～ 2 5岁 ,平均 13岁。单侧皮瓣 8例 ,双侧皮瓣 1例。最小皮瓣为 6 cm× 2 .0 cm,最大为 16 cm× 3.0 cm,皮瓣全部成活。 1例左耳根部瘢痕过厚 ,皮瓣末端有约 1.5 cm× 2 .5 cm大皮瓣坏死 ,经局部换药后创面愈合。1.2　手术方法　皮瓣蒂部在颈前区瘢痕外缘 (切除增生瘢痕 ,充分松解周围粘连 ) ,根据受区大小设计皮瓣 ,向肩胸部延伸 ,并较受区创面放大约 1～ 2 cm左右 ,宽长比例达 1:3～ 1:5…     

显微外科治疗23例前足及足跟部皮肤缺损的临床观察

目的总结足内侧皮瓣、足底内侧皮瓣修复前足及足跟部皮肤软组织缺损的临床效果。方法2002年6月至2008年8月采用足内侧皮瓣、足底内侧皮瓣修复前足及足跟部皮肤软组织缺损创面,临床应用23例,皮瓣面积为5 cm×3 cm~12 cm×7 cm。结果术后皮瓣全部成活,随访6~24个月,皮瓣质地良好,外形不臃肿,供区愈合良好。结论足内侧皮瓣、足底内侧皮瓣血运可靠,手术切取简便安全,是修复前足及足跟部皮肤软组织缺损创面的理想方法。     

足跟大面积皮肤缺损的显微外科治疗

目的 报道应用腓肠神经营养血管逆行岛状皮瓣及吻合血管的足背皮瓣修复足跟部较大面积皮肤缺损的临床效果.方法 临床治疗36例足跟及足踝部大面积皮肤缺损的病例,其中应用腓肠神经营养血管逆行岛状皮瓣修复足跟皮肤缺损25例,皮瓣面积最大30 cm×14 cm,最小11 cm×7 cm,应用吻合血管的足背皮瓣修复足跟皮肤缺损11例,皮瓣面积最大14 cm×11 cm,最小9 cm×6 cm.结果 皮瓣全部成活,术后有1例腓肠神经营养血管逆行岛状皮瓣远端少许皮肤坏死,经换药切痴后用中厚皮片植皮愈合.所有病例均经3个月～3 a随访,患肢正常步态行走,皮瓣耐磨,两点辨别觉4～7 cm.结论 腓肠神经营养血管逆行岛状皮瓣及足背皮瓣皮肤质量较好,不臃肿,供皮面积较大,适合修复足跟部较大面积皮肤缺损.     

外踝上逆行岛状筋膜皮瓣修复踝部软组织缺损13例

因外伤、炎症、肿瘤造成的踝部软组织缺损临床上并不少见,因为常伴有肌腱、骨骼、韧带的外露,使得创面修复有一定困难。我科自1995年1月￣2005年12月期间,应用外踝上逆行岛状筋膜皮瓣修复踝部软组织缺损13例,效果满意,报告如下。1临床资料本组13例,男9例,女4例,年龄23岁￣62岁,平均36岁。踝部软组织缺损部位:前方6例,内侧3例,跟腱处4例。软组织缺损范围:最大6.5cm×5cm,最小3.5cm×2.5cm。切取皮瓣范围:最大7.5cm×6cm,最小4.5cm×3.5cm。13例皮瓣全部成活。随访2个月￣半年,皮瓣外观与功能均满意。2手术方法2.1麻醉采用椎管内麻醉,病人取侧…     

足跟部皮肤缺损的显微外科修复

目的探讨足跟部组织缺损的显微外科修复方法及临床效果。方法分别采用足底内侧皮瓣、足底外侧皮瓣、足外侧皮瓣、足背皮瓣、内踝上皮瓣、腓肠神经营养血管皮瓣、游离股前外侧皮瓣修复足跟组织缺损17例,皮瓣均含有感觉神经,皮瓣切取面积6 cm×4 cm～14 cm×10 cm。结果17例皮瓣全部成活,经6～30个月随访,依据足跟的感觉和行走功能恢复情况进行综合评价,优8例(47.0%),良7例(41.2%),可2例(11.8%),优良率88.2%。结论足跟部组织缺损首选足底内侧皮瓣修复,创面较大的可选用小腿远端蒂皮瓣,局部皮瓣无法利用者,可考虑游离皮瓣。     

腓肠神经营养血管岛状皮瓣的临床应用

目的　总结逆行腓肠神经营养血管岛状皮瓣的临床应用经验。方法　以腓肠神经及其营养血管为蒂的逆行岛状皮瓣修复胫前、踝部、足跟部软组织缺损创面 2 3例 ,皮瓣大小为 ( 3cm× 5cm)～( 8cm× 1 5cm)。结果　 1 9例完全成活 ,3例出现水泡及边缘 0 .5cm左右皮肤坏死 ,经换药愈合 ,1例远侧部分皮肤坏死 ,移植游离皮片得以修复。 2 1例获随访 3～ 6个月 ,1 6例皮瓣恢复保护性感觉 ,另 5例感觉无恢复。无溃疡、瘘管、骨髓炎出现。结论　此皮瓣不损伤小腿重要血管及神经 ,血供可靠 ,解剖容易 ,是修复小腿中下部、踝部和足跟部软组织缺损的理想皮瓣     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.