脐带间充质干细胞移植对卵巢早衰模型小鼠卵巢功能的影响

目的探讨人脐带间充质干细胞(hUCMSCs)移植小鼠卵巢修复化学治疗药物诱导的卵巢早衰(POF)。方法选择雌性ICR小鼠40只,鼠龄8周,体质量25～30 g。将ICR小鼠采用SPSS生成随机数字分为空白对照组、POF组、h UCMSCs组。空白对照组不做任何处理,POF组与hUCMSCs组每天腹腔注射环磷酰胺(CTX)70 mg/mL,连续15 d,建立POF模型。建模成功后hUCMSCs组小鼠经尾静脉注射hUCMSCs进行治疗,POF组注射同等剂量的0.9%氯化钠溶液(生理盐水)。观察小鼠一般情况及体质量,并在移植后的7 d、14 d测定血清中雌激素(E2)、卵泡刺激素(FSH)、抗苗勒管激素(AMH)含量及观察卵巢组织形态改变。结果建模完成后,与空白对照组比较,POF组和hUCMSCs组血清E2、AMH均都降低,血清FSH升高,其差异均有统计学意义(P <0.05);hUCMSCs移植后7 d,与POF组比较[(94.39±14.99) mIU/mL、(60.92±14.60) pg/mL、(2 870.00±801.74) pg/mL],hUCMSCs组血清FSH[(80...     

人脐带间充质干细胞的研究进展

脐带间充质干细胞是存在于脐带沃顿胶和血管周围组织中的一种干细胞,具有多向分化和自我更新的潜能。脐带间充质干细胞可分化为骨细胞、软骨细胞、脂肪细胞、肌细胞和神经细胞,并且具有免疫调节性;脐带作为医疗废弃物来源丰富,对供者无不利影响,无伦理问题的限制,这为细胞治疗和组织工程提供了新的种子细胞。     

人脐带间充质干细胞移植治疗系统性红斑狼疮

背景：系统性红斑狼疮是一种以多器官或多系统病变和血清中出现多种自身抗体为特征的自身免疫性疾病,目前缺乏有效的治疗方案,而理论上间充质干细胞可用于治疗系统性红斑狼疮。目的：观察人脐带间充质干细胞移植治疗系统性红斑狼疮小鼠的疗效。方法：分离培养人脐带间充质干细胞,并用深红色荧光DiR标记细胞。实验小鼠分5组：正常对照组(C57BL小鼠),模型对照组(C57BL/lpr小鼠),低、中、高剂量脐带间充质干细胞治疗组(C57BL/lpr小鼠),每组10只。各治疗组通过尾静脉注射低、中、高剂量(2×10⁶,1×10⁶,0.5×10⁶个)脐带间充质干细胞,每周1次,连续3周,治疗结束采血测抗核抗体、抗组蛋白抗体、抗双链DNA抗体变化,定量PCR检测OPG和Foxp3基因表达的变化。结果与结论：细胞移植3次后,外周血抗核抗体、抗组蛋白抗体、抗双链DNA抗体均明显下降,CD4⁺CD25⁺T细胞明显升高,OPG和Foxp3基因表达也明显升高,接近正常对照组,与模型对照组相比差异均有显著性意义(P < 0.01)。结果表明人脐带间充质干细胞能使C57BL/lpr小鼠的各项相关指标恢复到C57BL正常鼠水平,以高剂量治疗组效果最明显。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：     

移植途径对人脐带间充质干细胞治疗小鼠糖尿病效果的影响

目的:观察不同途径移植人脐带间充质干细胞(hUCMSCs)对小鼠糖尿病的治疗效果。方法:利用增强绿色荧光蛋白和萤光素酶报告系统(EGFP/Luc)标记hUCMSCs,通过胰腺包膜下途径或尾静脉途径将携带萤光标记的hUCMSCs移植到链脲霉素诱导的糖尿病模型小鼠体内。移植后利用萤光素酶报告基因追踪hUCMSCs在活体内的迁移和定位;组织学检测小鼠胰岛形态变化;功能学实验动态检测小鼠血糖、血清胰岛素水平和糖耐量。结果:活体生物发光成像显示胰腺包膜下途径移植的hUCMSCs主要定位于胰腺,尾静脉途径移植的hUCMSCs主要定位于肺,仅少量细胞向胰腺部位迁移。组织学检测发现,胰腺包膜下途径移植的小鼠胰岛边界清晰,无炎症细胞浸润;而尾静脉途径移植的小鼠胰腺组织有少量炎症细胞浸润和纤维化形成。功能学检测发现胰腺包膜下移植较尾静脉移植降低小鼠血糖作用显著,血糖可降至接近正常水平,且血清胰岛素水平明显升高,葡萄糖的调节能力显著增强。结论:移植途径对hUCMSCs治疗糖尿病的效果有影响。胰腺包膜下移植在降低小鼠血糖、升高胰岛素水平及改善胰岛功能方面均优于尾静脉移植。     

人脐带间充质干细胞移植治疗心肌肥厚模型小鼠

背景:左心室肥厚作为高血压病常见的靶器官损害,是心脑血管疾病高发病率和死亡率的独立危险因素。间充质干细胞可以促进组织修复,调节免疫反应,可作为治疗心肌肥厚的种子细胞。目的:观察人脐带间充质干细胞静脉注射治疗小鼠心肌肥厚的疗效。方法:(1)将C57BL/6J小鼠随机分为假手术组、人脐带间充质干细胞组、PBS组,其中人脐带间充质干细胞组和PBS组采用主动脉弓缩窄术构建C57BL/6J小鼠心肌肥厚模型,人脐带间充质干细胞组小鼠术后第5周开始,尾静脉注射细胞混悬液,每周1次,连续4周,PBS组小鼠尾静脉注射等量PBS,术后6-11周采用超声心动图观察各组小鼠心功能,术后9,10,11周采用苏木精-伊红染色、Western blot检测心肌细胞肥大程度;(2)体外实验中,通过异丙肾上腺素诱导H9c2细胞肥大,然后将人脐带间充质干细胞与肥大的H9c2细胞共培养于Transwell^TM小室中,共培养48 h后F-actin染色分析H9c2细胞横截面积,qRT-PCR检测H9c2细胞内心房钠尿肽、脑钠尿肽和β-肌球蛋白重链m RNA表达量。结果与结论:(1)超声心动图结果证实:人...     

人脐带间充质干细胞移植治疗急性肺损伤

BACKGROUND:Studies have shown that human umbilical cord mesenchymal stem cells can improve pulmonary ventilation function by reducing inflammations. OBJECTIVE:To observe the therapeutic effect of human umbilical cord mesenchymal stem cell transplantation on acute lung injury. METHODS:Thirty Sprague-Dawley rats were randomized into normal group, model group and experimental group. Rats in the latter two groups were used to establish animal models of acute lung injury by intratracheal instillation of lipopolysaccharide. One hour after modeling, rats in the experimental group were intratracheally administered human umbilical cord mesenchymal stem cell suspension (0.1 mL, 1×10⁶ cells), and those in the other two groups were given normal saline in the same dose intratracheally. Twenty-four hours after treatment, the pathological changes of lung tissue were observed using hematoxylin-eosin staining; the wet and dry weight ratio of the lung tissue and the levels of serum interleukin-1 and interleukin-8 were detected. RESULTS AND CONCLUSION:Compared with the normal group, the wet and dry weight ratio of the lung tissue and the levels of serum interleukin-1 and interleukin-8 were significantly increased in the model group (P < 0.05), while compared with the model group, these levels were significantly decreased in the experimental group (P < 0.05). Hematoxylin-eosin staining results showed clear alveolar space structure with complete alveolar septum in the normal group. In the model group, the alveolar septum was markedly thickened, and there was visible pulmonary capillary hyperemia, edema, as well as a large amount of inflammatory cell infiltrations in the pulmonary capillaries and alveolar space. Edema fluid rich in proteins was observed in a part of the pulmonary alveoli, and an extensive transparent membrane formed in the alveolar space. In the experimental group, the alveolar structure was clear, but the alveolar septum became thickened, and red blood cells and a small amount of infiltrated inflammatory cells were leaked from the pulmonary interstitial tissue. In conclusion, human umbilical cord mesenchymal stem cell transplantation for treatment of acute lung injury can reduce inflammatory factor levels and alleviate lung injury.     

脐带间充质干细胞移植对脊髓损伤小鼠运动功能的修复和镇痛作用

目的：探讨人脐带间充质干细胞（ hUC-MSCs）移植缓解脊髓损伤神经病理性痛,并促进功能恢复的效果及 其与脊髓损伤小鼠胶质细胞活化及炎症因子水平的调控关系。方法：建立ICR 小鼠脊髓损伤模型,同时构建慢病毒 载体介导绿色荧光蛋白（ GFP）标记体外培养的 hUC-MSCs ;将模型小鼠分为模型组和治疗组,治疗组于脊髓损 伤1 周后采用局部注射 hUC-MSCs 移植到脊髓中,每周进行运动功能（ BBB）评分及机械性痛觉过敏检测,持续8 周后行组织学评价与炎症因子检测。结果：治疗组脊髓组织中 GFP 荧光有表达。BBB检测结果显示,治疗组和模 型组小鼠随时间延长运动功能均逐渐恢复,其中治疗组运动功能恢复速度要显著快于模型组;机械触诱发痛检测显 示,小鼠脊髓损伤后痛阈值降低,随着时间延长痛阈值逐渐升高。同一个时间点治疗组的痛阈值显著高于模型组。 与模型组相比,治疗组小鼠脊髓组织的白细胞介素-6（ IL-6）、肿瘤坏死因子-α（ TNF-α）表达下降,胶质细胞源 性神经营养因子（ GDNF）表达升高,同时脊髓组织巨噬细胞激活抗原1（ ED1/CD68） 荧光表达显著降低。结论： 脊髓损伤小鼠中hUC-MSCs 移植可能通过降低炎症因子 IL-6 和TNF-α 的分泌,提高 GDNF 的表达水平来促进受损 脊髓组织的修复,并发挥镇痛效应。     

临床级人脐带间充质干细胞资源库的构建

目的:建立临床级人脐带间充质干细胞（hUC-MSCs）资源库三级库,即种子细胞库、主细胞库和工作细胞库,为干细胞临床研究和转化应用提供质量可控的临床级hUC-MSCs资源。方法:将通过筛选入组的247份人脐带组织于药品生产质量管理规范（GMP）实验室进行分离、培养、扩增、传代、冻存等一系列操作,并按照质量管理控制相关规...     

人脐带间充质干细胞的生物学性质研究进展

人脐带中富含间充质干细胞(MSCs)。这些细胞能表达多种间充质干细胞标志物及多种干细胞相关基因,能分化为3个胚层衍生的多种成熟细胞,合成多种营养因子和细胞因子,支持造血干细胞等细胞的增殖和功能,并具有低免疫原性。     

长期培养人脐带间充质干细胞的生物活性及其限制性

背景：间充质干细胞具有多向分化能力、免疫调节能力,并可在体外进行长期培养扩增,但其体外长期培养的生物学活性变化特点及其限制性仍不十分清晰。 目的：观察体外长期培养人脐带间充质干细胞的生物活性变化特点及其限制性。 方法：无菌收集剖宫产新生儿脐带,经胶原酶Ⅱ消化后,用黏附生长选择法获取人脐带间充质干细胞,胰酶消化传代;取第3代细胞进行流式分析,脂肪和成骨诱导鉴定;收集不同代的细胞分别用MTT法、流式细胞仪和爬片分析法检测细胞增殖活性、细胞周期、凋亡率及黏附能力。 结果与结论：人脐带间充质干细胞的细胞表型CD105⁺/CD29⁺/CD44+/CD31^-/CD34^-/CD45^-/HLADR^-,可诱导分化为脂肪细胞和成骨细胞;传代培养第3~23代细胞形态无明显变化,生长曲线基本一致,第28代后细胞增长缓慢;流式分析发现第33代细胞较第3代G0/G1减少17.9%、S+G2/M增加103.4%、细胞凋亡率增加316.7%,差异均有显著性意义(P < 0.01),第3~18代细胞间G0/G1、S+G2/M、细胞凋亡率和黏附细胞数差异均无显著性意义(P > 0.05)。提示在体外长期培养环境中,随着传代次数的递增,人脐带间充质干细胞的增殖及黏附能力下降,细胞凋亡率增加,总的生物活性呈逐渐下降趋势,其最佳生物活性期出现在培养第3~18代之间。     

Therapeutic effect of human umbilical cord-derived mesenchymal stem cells in rat severe acute pancreatitis

Aim: To investigate the therapeutic effect of umbilical cord-derived mesenchymal stem cells (UC-MSCs) on rat severe acute pancreatitis (SAP). Methods: Rats were randomly divided into three groups (n = 15 per group): control group, SAP group, and SAP+MSCs group. SAP was established by retrograde pancreatic duct injection of 3% sodium taurocholate. In SAP+MSCs group, UC-MSCs at 1×10⁷ cells/kg were injected via the tail vein 12 h after SAP. Rats (n = 5 per group) were sacrificed on days 1, 3 and 5, and the blood and pancreatic tissues were collected. The levels of serum amylase, lipase, inflammatory cytokines, and anti-inflammatory cytokines were determined. Pathological changes of the pancreas (HE staining) and apoptotic acinar cells (TUNEL staining) were observed under light microscope. Results: The levels of serum amylase and lipase in SAP group were significantly higher than those in control group (P<0.05). The pancreas in SAP group showed significantly massive edema, inflammation, hemorrhage and necrosis when compared with control group. There were numerous TUNEL-positive apoptotic acinar cells after SAP. However, in SAP+MSCs group, the levels of serum amylase were significantly reduced on days 1, 3, and 5 after MSC transplantation (P<0.01). The serum lipase level in SAP+MSCs group was significantly lower than that in SAP group on days 3 and 5 (P<0.01). The edema formation, inflammatory cell infiltration, hemorrhage, and necrosis were reduced significantly attenuated in SAP+MSCs group as compared to SAP group (P<0.05). MSCs significantly reduced the levels of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6), but increased the levels of anti-inflammatory cytokines (IL-4 and IL-10) in SAP rats. The number of TUNEL-positive acinar cells was significantly reduced on days 3 and 5 after MSCs transplantation (P<0.01). Conclusion: Transplantation of UC-MSCs significantly inhibits inflammation and decreases pancreatic injury secondary to SAP.     

人脐带间充质干细胞冻存复苏后细胞表面标志物分子学变化

背景：间充质干细胞是具有自我更新能力且能够多向分化的干细胞。脐带属于胚胎外组织,是胎儿分娩后的废弃物,来源广泛,无伦理学限制,因此有望作为间充质干细胞来源的第一选择。 目的：检测人脐带间充质干细胞冻存复苏前后细胞表面分子CD29、CD44、CD49e、CD73、CD90、CD34、CD45、CD271标志变化情况。 方法：通过从人的脐带中分离培养间充质干细胞,分别观察原代细胞、P4、P8代细胞和冻存复苏后P2、P4、P8代细胞形态;通过流式细胞仪检测原代细胞、P4、P8代细胞和冻存复苏后P2、P4、P8代细胞表面分子标志CD29、CD44、CD49e、CD73、CD90、CD34、CD45、CD271。 结果与结论：人脐带间充质干细胞冻存前与原代复苏后不同代数下细胞均表现出相同的表型,CD29、CD44、CD49e、CD73、CD90阳性,CD34、CD45、CD271阴性,提示人脐带间充质干细胞原代低温冻存复苏后细胞表面标志性分子无变化。     

人脐带源间充质干细胞静脉移植治疗大鼠肝纤维化

背景：近来国内外研究证明,来自其他组织的干细胞能够归巢到肝脏,并可能参与肝组织的再生,这为发展干细胞治疗肝脏疾病提供了新的希望。 目的：探究人脐带源间充质干细胞的分离、培养方法,观察脐带间充质干细胞移植对大鼠肝纤维化模型的修复作用,为脐带间充质干细胞的临床应用提供可靠的理论依据。 方法：自然贴壁法分离、纯化人脐带间充质干细胞并进行体外培养和扩增,用皮下多点注射CCl₄制备肝纤维化大鼠模型。将22只模型大鼠随机分为模型损伤组(n=11)和细胞移植组(n=11)。细胞移植组在模型制备成功后的第1,2,3周经尾静脉给予1×10⁶脐带间充质干细胞治疗,4周后将大鼠处死,收集各组大鼠血液检测肝功能;摘取肝脏行苏木精-伊红染色,观察病理变化;免疫组化法观察库普弗细胞的数量及分布;免疫组化法观察治疗组脐带间充质干细胞的定位情况。 结果与结论：脐带间充质干细胞经尾静脉移植入肝硬化大鼠后,大鼠的肝功能均明显改善,与对照组比较,差异有显著性意义(P < 0.05);肝组织苏木精-伊红染色提示,肝纤维化程度明显改善;免疫组化法观察库普弗细胞的数量提示,库普弗细胞数量明显减少;免疫组化方法利用抗BrdU抗体在治疗组大鼠肝脏观察到BrdU标记的脐带间充质干细胞。说明脐带间充质干细胞移植可以改善大鼠外周血液的血生化特性和肝的组织学结构。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：     

脐带来源间充质干细胞的制备及其质量检定

目的：研究脐带来源间充质干细胞（UC-MSCs）的扩增工艺及质量检验方法,提出质量控制标准,为临床研究提供合格的干细胞产品。方法新鲜脐带去羊膜及血管,剪碎,组织块放入无菌培养皿,在培养箱进行脐带间充质干细胞培养、扩增。细胞培养至 P3代,按照《干细胞制剂质量控制及临床前研究指导原则》（征求意见稿）进行干细胞质量的全面检定。检定内容包括无菌检测,支原体检测,人源特定病毒及猪源病毒检测,内毒素检测,细胞形态、细胞数及细胞活率检测,染色体核型分析,干细胞免疫表型检测,STR 图谱分析,免疫抑制活性检测及分化能力检测。结果按照标准化流程对18根脐带进行了 UC-MSCs 的分离、纯化及培养,获得一致性较好的干细胞。通过对干细胞进行质量检定,很好地控制了干细胞的质量。干细胞活性在冻存前≥90%,冻存复苏后≥80%;该工艺生产的脐带MSC 产品无细菌、人源特定病毒及猪细小病毒、支原体等微生物污染,内毒素检测阴性;干细胞免疫表型检测 CD90、CD105呈阳性,阳性率不低于95%;CD31、CD34、HLA-DR呈阴性,阳性率不高于2%;染色体核型分析为46XX 或46XY,无缺失及插入突变;STR 图谱分析证实干细胞为单一人来源;UC-MSCs 具有成脂及成骨分化潜能;UC-MSCs能抑制异源淋巴细胞增殖。结论按本工艺及标准制备的 UC-MSCs 符合《干细胞制剂质量控制及临床前研究指导原则》的质量控制标准,为同类干细胞制备及检定过程标准化提供了实验依据。     

神经干细胞移植治疗老年痴呆

BACKGROUND:Neural stem cell transplantation has been used to treat a series of brain injury diseases, such as cerebral palsy, but its effect on Alzheimer’s disease is rarely reported. OBJECTIVE:To observe the effect of neural stem cell transplantation on the behavior and immune regulating system of Alzheimer’s disease rats.  METHODS:Thirty-five Sprague-Dawley rats were enrolled to make a postcerebral incision and given hippocampal injection of amanita phalloides acid to establish rat models of Alzheimer’s disease. Another 10 rats were only given hippocampal injection of normal saline after preparation of postcerebral skin incision as sham operation group. Then 32 successful rat models were randomly divided into two groups (n=16 per group): rats in experimental group were administrated hippocamal injection of 5×109/L allogeneic neural stem cell suspension; those in model group were given no injection. Five-day Morris water maze test was conducted at 4 weeks after transplantation. At 1 week after Morris water maze test, levels of interleukin-1 and interleukin-10 in the cerebral homogenate were detected, as well as pathological changes of brain tissues were observed in the three groups. RESULTS AND CONCLUSION:Compared with the model group, the abilities of cognition and memory were significantly higher in the sham operation group (P < 0.01), and the abilities of spatial learning and memory were significantly higher in the experimental group (P < 0.05, P < 0.01). Levels of interleukin-1 and interleukin-10 in the model group were significantly higher than those in the sham operation group (P < 0.01) but significantly lower than those in the experimental group (P < 0.01). Besides, the number of neurons in the model group was obviously less than that in the experimental and sham operation group. These results indicate that neural stem cell transplantation supplements and protects neurons against Alzheimer's disease in rats, thereby significantly improving the learning and memory ability.     

缬沙坦诱导人脐带间充质干细胞向心肌样细胞分化

目的 探讨缬沙坦诱导人脐带间充质干细胞（hUCMSCs）向心肌样细胞分化的潜能。方法 hUCMSCs经缬沙坦诱导后培养1～3 周, 应用心肌肌钙蛋白T（cTnT）和GATA结合蛋白4重组蛋白（GATA4）抗体进行免疫细胞化学和免疫荧光染色,鉴定分化后的细胞。通过RT-PCR检测心肌肌钙蛋白I(cTnI)基因、心肌相关转录因子和心肌细胞发育相关基因的mRNA表达,进行hUCMSCs的心肌分化潜能和缬沙坦诱导能力的分析。结果 人脐带间充质干细胞形态为均一的纤维状细胞,呈漩涡状排列。缬沙坦诱导后明显变小呈短梭形且不规则排列。免疫细胞化学和免疫荧光结果显示,诱导组细胞有心肌特异蛋白cTnI和心肌相关转录因子GATA4蛋白表达,对照组较少。RT-PCR结果显示,人脐带间充质干细胞自发的、持续性表达心肌相关转录因子Tbx5、GATA4和Nkx2.5的mRNA,经缬沙坦诱导1周时其mRNA表达水平略有上升,随后逐渐下降。cTnI的mRNA在诱导第3周时出现,未见心肌发育相关基因心房利钠肽（ANP）和β-心肌肌球蛋白重链（β-MHC）的mRNA表达。 结论 人脐带间充质干细胞具有心肌分化潜能但无法自发分化;缬沙坦具有诱导人脐带间充质干细胞向心肌样细胞分化的能力。     

人脐血间充质干细胞移植对脑创伤大鼠的免疫调节研究

目的 探讨人脐血间充质干细胞(HUCBMSCs)移植对脑损伤大鼠的免疫调节和脑保护作用机制,为脑损伤的干细胞治疗提供依据.方法 用Brdu标记的HUCBMSCs经鼠尾静脉植入大鼠创伤性脑损伤模型.采用NSS方法行神经损伤严重程度评分.正常组不作任何处理,在造模后第1天、第2天、第3天、第5天和第7天检测和比较假伤组、损伤组和移植组大鼠血清免疫指标的变化(炎症介质、全血在脂多糖刺激后合成TNF-α的能力).结果 Brdu阳性的MSCs在移植组大鼠脑损伤周边区明显聚集.除第1天外,各时间点移植组大鼠神经功能得分较损伤组降低,差异有统计学意义(t=3.35、5.70、5.40、4.93,P均＜0.05).移植组大鼠在损伤后各个时间点血清IL-6水平较损伤组显著降低(t=4.72、3.53、3.77、2.32、2.43,P均＜0.05),在损伤后第1天血清IL-8水平较损伤组显著降低(t=2.26,P＜0.05),而移植组IL-10经历短暂下降后出现明显的回升趋势,在后期(第5天和第7天)血清IL-10水平甚至高于假伤组(t=2.61、2.81,P均＜0.05).移植组大鼠全血体外合成TNF-α能力在损伤早期虽然出现迅速下调,但中后期可以观察到这个指标上调的趋势,均高于同时间点损伤组大鼠水平(t=2.12、2.53、2.77、4.32、7.43,P均＜0.05).结论 未应用免疫抑制剂的情况下,经尾静脉注射HUCBMSCs可迁移至免疫功能健全的大鼠脑创伤灶周边区,HUCBMSCs能降低严重创伤的高炎性反应,并有效扭转创伤后的免疫抑制状态,有助于神经功能改善.     

人脐带间充质干细胞移植治疗大鼠急性肺损伤

背景：间充质干细胞具有免疫调节特性,脐带间充质干细胞因其特有的优势,将在急性肺损伤和急性呼吸窘迫综合征的临床应用中有着光明的前景。 目的：探讨脐带间充质干细胞移植对内毒素性大鼠急性肺损伤模型的保护作用。 方法：将48只健康雄性SD大鼠随机均分为正常对照组、急性肺损伤组和脐带间充质干细胞移植组。后两组采用经气管内滴注内毒素建立急性肺损伤模型。成功造模1 h后,脐带间充质干细胞移植组,经气管内滴注脐带间充质干细胞混悬液,正常对照组和急性肺损伤组同法予以等量生理盐水。分别在干预后24,72 h,观察肺组织病理改变,检测病理组织评分、肺组织干湿质量比、髓过氧化物酶活性及血浆白细胞介素6、白细胞介素10及肿瘤坏死因子α水平。 结果与结论：脐带间充质干细胞移植可以减轻内毒素诱导的急性肺损伤模型的损伤程度;脐带间充质干细胞移植组在各时间点与急性肺损伤组比较,病理损伤评分、肺干湿质量比、肺组织髓过氧化物酶活性、血浆白细胞介素6和肿瘤坏死因子α水平均明显降低,血浆白细胞介素10水平明显升高。说明脐带间充质干细胞对内毒素性急性肺损伤模型有保护作用;其保护机制可能为脐带间充质干细胞移植维持肺内炎性递质和抗炎递质平衡。     

脐血干细胞移植对帕金森病大鼠旋转行为的影响

背景：目前帕金森病的临床治疗还是以药物为主,细胞移植实验也多见于骨髓间充质干细胞,脐血来源干细胞移植能否改善帕金森病的旋转行为报道较少。 目的：观察脐血间充质干细胞移植对帕金森病大鼠旋转行为的影响。 方法：帕金森病模型大鼠随机分成实验组和对照组。实验组大鼠纹状体内植入用Hoechst33258标记的第4代脐血间充质干细胞,对照组注射PBS。此后每周腹腔注射阿扑吗啡以观察大鼠的旋转行为;并在移植后3,6,9周用免疫荧光双标法检测间充质干细胞的存活、迁移情况以及胶质纤维酸性蛋白、神经元特异性烯醇化酶、酪氨酸羟化酶和突触素的表达。 结果与结论：移植脐血间充质干细胞后大鼠的旋转行为与对照组相比有明显改善(P < 0.05);间充质干细胞可在大鼠脑内存活,随时间延长迁移范围扩大,分布于纹状体、胼胝体和皮质;胶质纤维酸性蛋白、神经元特异性烯醇化酶、酪氨酸羟化酶都有表达,突触素无表达。结果可见移植脐血间充质干细胞后能明显改善帕金森病大鼠旋转行为,有望成为治疗帕金森病的种子细胞。      

胚胎神经干细胞移植治疗阿尔茨海默病

BACKGROUND:More recently, stem cell therapy has become an issue of concern. Exogenous neural stem cell transplantation brings new hope for the treatment of nervous system injury by self-replication and differentiation to complement and replace damaged or dead nerve cells. OBJECTIVE:To explore the therapeutic efficacy of neural stem cell transplantation on Alzheimer’s disease. METHODS:Thirty APP/PS1 mice with Alzheimer’s disease were randomly assigned into model group, cell solution transplantation group or cell transplantation group (n=10 per group). Another 10 C57BL/6 mice were selected as controls. Embryos of C57BL/6 mice at 18 embryonic days were taken to make neural stem cell suspension followed by transfection using lentiviral vectors carrying GFP gene at different multiplicities of infection (1, 5, 10, 15, 20). Afterwards, GFP-transfected neural stem cells were implanted into the hippocampus of Alzheimer’s disease mice in the cell transplantation group, while the same volume of complete medium was injected into the hippocampus of mice in the cell solution transplantation group. Morris water maze test was performed at 2 weeks after cell transplantation, and brain tissues of mice was taken and detected histologically at 4 weeks after cell transplantation. RESULTS AND CONCLUSION:Compared with the control group, the escape latency was significantly higher, and the number of crossings over the target quadrant was lower in the other three groups (P < 0.05). Compared with the cell solution transplantation and model groups, in contrast, the escape latency was significantly lower, and the number of crossings over the target quadrant was significantly higher in the cell transplantation group (P < 0.05). Four weeks after transplantation, more intact neurons were found in the cell transplantation group as compared with the model group. These findings indicate that neural stem cell transplantation can improve behavior and morphology performance of mice with Alzheimer’s disease.