骨髓间充质干细胞移植治疗缺血性脑卒中的研究进展

骨髓间充质干细胞（BMSCs）是一种具有多向分化和增殖潜能的干细胞,在体内、外均能分化出神经细胞。近年来,BMSCs移植治疗缺血性脑卒中的动物实验和临床研究都取得了较好效果,已成为治疗神经系统疾病的一种新技术,具有广阔的发展前景。本文将就骨髓间充质干细胞的生物学特征及其移植治疗缺血性脑卒中的作用及机理予以综述。     

骨髓间充质干细胞移植治疗缺氧缺血性脑损伤的现状及进展

骨髓间充质干细胞是中胚层来源的具有自我更新和多项分化潜能的多能干细胞,在适宜的培养条件下可被诱导分化。有证据表明,骨髓间充质干细胞注射入脑后在体内外诸多影响因素作用下,可分化为神经元和成熟的胶质细胞。骨髓间充质干细胞的易获得性,多分化潜能等生物特性,使它在缺氧缺血性脑损伤中显示了巨大的潜在治疗价值。     

骨髓间充质干细胞移植治疗缺血性脑损伤的最新研究进展

脊髓间充质干细胞是一类多潜能的干细胞,它被移植入脑缺血灶后在一定程度上可以转化成为神经元和胶质细胞,从而发挥着替代受损的神经组织的功能。脑缺血是一种发病急且后果比较严重的疾病,探索干细胞移植治疗对缺血性脑损伤的作用具有重要的意义。     

骨髓间充质干细胞移植治疗肝硬化的研究进展

肝硬化是临床中常见的慢性进行性肝病,目前治疗晚期肝硬化最有效的方法是肝脏移植,但肝源缺乏、费用昂贵、移植排斥反应及长期应用免疫抑制剂引起并发症等成为限制其广泛应用的主要原因.干细胞移植有利于受损肝组织修复,能够代偿部分肝功能,已成为治疗肝病的一种新方法.就骨髓间充质干细胞移植治疗肝硬化的基础、临床研究进展、存在的问题以及发展前景作一综述,旨在为其进一步研究提供理论依据.     

骨髓间充质干细胞移植治疗骨质疏松的研究进展

骨质疏松症被定义为一种系统性骨骼疾病,其特征是骨量低、骨组织微结构恶化、骨脆性和骨折易感性增加。 目前有2亿多人患有骨质疏松症,但由于人口老龄化和人均寿命延长,受影响的人数仍在急剧增加,这是一个重大的公共卫生问题。目前,治疗骨质疏松症的药物开发已经取得了重大进展,但药物治疗并不能逆转骨丢失,且会给患者带来一系列毒副作用。大量研究表明,骨髓间充质干细胞的归巢作用、成骨分化和细胞因子作用在骨质疏松发病过程中发挥重要作用。 移植骨髓间充质干细胞作为一种新方法,不仅能避开药物治疗的副作用而且能从根本上治疗骨质疏松,具有巨大的潜能和应用价值,但许多问题也有待解决。     

不同密度骨髓间充质干细胞移植对家兔股骨头缺血性坏死的治疗作用

目的比较不同密度的骨髓间充质干细胞(BMSCs)移植,对实验性家兔股骨头缺血性坏死(ANFH)的治疗作用,探索合适的BMSCs移植密度。方法选取成年健康新西兰大耳白兔40只,根据BMSCs移植密度随机分为1×104(A),1×105(B),1×106(C),1×107(D),1×108(E)/ml BMSCs5组。对术后2、4、6、8周的股骨头标本,进行X-射线检查、组织学观察和图象分析。结果X-射线观察显示:随着时间的进展,各组钻孔区密度逐渐增加,A组密度增加不均,8周时,B、C、D、E组钻孔区呈现正常的骨小梁结构,A组部分钻孔区域呈现低密度腔隙。组织学观察表明:术后2周时,各组钻孔区内出现大量的成骨细胞,A组钻孔区中心为炎性细胞;8周时B、C、D、E组钻孔区内,骨小梁趋于成熟。A组钻孔区内分布有不均匀的骨小梁和骨髓组织。图像分析表明:在B、C、D、E组钻孔区内骨小梁的面积百分比值明显高于A组。结论体外培养的BMSCs移植修复ANFH时,不宜选择104以下的密度级别,应该尽可能提高移植细胞的密度。     

高压氧联合骨髓间充质干细胞移植修复缺氧缺血性脑损伤

背景：单纯骨髓间充质干细胞移植对脑梗死组织的修复作用并不理想,需要结合药物及生物工程材料等手段进行综合治疗。 目的：验证高压氧结合骨髓间充质干细胞移植修复大鼠缺氧缺血性脑损伤的效果。 方法：体外培养大鼠骨髓间充质干细胞。应用线栓法建立大脑中动脉阻塞大鼠模型,按随机区组法分为3组,即对照组、骨髓间充质干细胞移植组及高压氧+骨髓间充质干细胞移植组。静脉移植后24 h,3 d及伤后1,2 周行Longa行为学评分,检测神经功能的损伤情况。移植2周后,应用RT-PCR法测定生长相关蛋白43 mRNA的表达,并以BrdU免疫组化和苏木精-伊红染色行梗死处组织学检查以证实恢复程度。 结果与结论：移植后1周,高压氧+骨髓间充质干细胞移植组大鼠神经功能障碍评分低于骨髓间充质干细胞移植组,骨髓间充质干细胞移植组低于对照组(P  < 0.05)。2周后脑梗死周围组织生长相关蛋白43 mRNA的表达高压氧+骨髓间充质干细胞移植组高于骨髓间充质干细胞移植组,骨髓间充质干细胞移植组高于对照组(P < 0.05)。BrdU免疫组化和苏木精-伊红切片中的神经元数量高压氧+骨髓间充质干细胞移植组多于骨髓间充质干细胞移植组,骨髓间充质干细胞移植组多于对照组(P < 0.05)。提示高压氧联合骨髓间充质干细胞静脉移植治疗大鼠缺氧缺血性脑损伤可明显改善大鼠的神经功能,效果优于单纯骨髓间充质干细胞移植。     

骨髓间充质干细胞经大鼠冠状动脉内移植可行性研究

目的 研究骨髓间充质干细胞经冠状动脉内移植的安全性及可行性。方法 左侧开胸结扎SD大鼠左冠状动脉，2周后取心脏建立Langerndorff模型。将CM-DiI标记的骨髓间充质干细胞悬液注入主动脉根部，同时收集右心房回流的液体，离心后通过流式细胞仪计数细胞数量。观察是否有细胞经冠状静脉回流及数量比例。并在不同时间测LVSP、LVDP、±dp/dt、心率，评价其安全性。在体实验中取心梗后2周的大鼠，经左心室--主动脉途径将细胞悬液注射到临时阻断的主动脉根部，分别在移植后1及24h和1及4周取材观察细胞在心脏内的位置及细胞的迁移情况。结果 离体实验发现经冠状动脉内注射髓间充质干细胞90%以上的细胞在心肌组织内存留，移植后对LVSP、LVDP、±dp/dt、心率没有明显变化。在体内实验中发现细胞移植后早期大部分细胞分布在心外膜下心肌组织内，在心内膜下组织内较少细胞分布，而且大部分细胞在正常心肌组织内，只有少量在心梗区域。而在细胞移植后1~ 4周存活的细胞多在心肌梗死及交界区组织内，在正常组织内很少有移植细胞存在。结论 经冠状动脉内途径进行细胞移植是安全可行的。     

骨髓间充质干细胞经大鼠冠状动脉内移植的可行性

目的 研究骨髓间充质干细胞经冠状动脉内移植的安全性及可行性.方法 左侧开胸结扎SD大鼠左冠状动脉,2周后取心脏建立Langerndorff模型.将CM-DiI标记的骨髓间充质干细胞悬液注入主动脉根部,同时收集右心房回流的液体,离心后通过流式细胞仪计数细胞数量.观察是否有细胞经冠状静脉回流及数量比例.并在不同时间测LVSP、LVDP、±dp/dt、心率,评价其安全性.在体实验中取心梗后2周的大鼠,经左心室-主动脉途径将细胞悬液注射到临时阻断的主动脉根部,分别在移植后1及24 h和1及4周取材观察细胞在心脏内的位置及细胞的迁移情况.结果 离体实验发现经冠状动脉内注射骨髓间充质干细胞90%以上的细胞在心肌组织内存留,移植后LVSP、LVDP、±dp/dt、心率没有明显变化.在体内实验中发现细胞移植后早期大部分细胞分布在心外膜下心肌组织内,在心内膜下组织内较少细胞分布,而且大部分细胞在正常心肌组织内,只有少量在心梗区域.而在细胞移植后1～4周存活的细胞多在心肌梗死及交界区组织内,在正常组织内很少有移植细胞存在.结论 经冠状动脉途径进行细胞移植是安全可行的.     

骨髓间充质干细胞移植治疗脊髓损伤的研究进展

脊髓损伤（spinal cord injury,SCI）是一种以损伤平面以下感觉、运动功能完全丧失和大小便失禁为主要临床表现的中枢神经系统严重创伤。近年来,随着干细胞生物学的发展,干细胞移植为临床治疗中枢神经系统损伤提供了新的途径,并有望成为促进神经损伤再生积极有效的方法。     

骨髓间充质干细胞移植治疗骨关节炎

BACKGROUND:As the ability of self-renewal, differentiation and migration into damaged tissues, bone marrow mesenchymal stem cells have been widely used in a variety of diseases. OBJECTIVE:To explore the therapeutic effect of bone marrow mesenchymal stem cell transplantation on osteoarthritis in rats. METHODS:Thirty-six Wistar rats were randomly assigned into transplantation, model or control group. osteoarthritis models were established in the transplantation and model groups, followed by tail vein injection of bone marrow mesenchymal stem cells (5×10⁷/kg) or the same volume of normal saline, respectively. Rats in the control group were subjected to no treatment. Four weeks after injection, levels of CD4⁺CD25⁺ regulatory T cells in the spleen, interleukin-17, tumor necrosis factor-α and transforming growth factor-β1 in serum were detected, and arthritis index and the degree of joint swelling were evaluated as well. RESULTS AND CONCLUSION:Compared with the control group, both arthritis index and degree of joint swelling were increased significantly in the model group (P < 0.05), but these indicators exhibited a remarkable improvement after cell transplantation (P < 0.05). Levels of CD4⁺CD25⁺ regulatory T cells in the spleen in the three groups were ranked as follows: the transplantation group > the control group > the model group. The levels of interleukin-17 and tumor necrosis factor-α in serum in the transplantation group were lower than those in the model group but higher than those in the control group (P < 0.05). Highest level of transforming growth factor-β1 was obtained in the transplantation group, followed by the control group and model group (P < 0.05). To conclude, these findings indicate that bone marrow mesenchymal stem cell transplantation exert therapeutic effects in osteoarthritis rats by immune and cytokine regulation.     

骨髓间充质干细胞移植治疗顺铂致卵巢的化疗性损伤

背景：化疗药物可使处于生育年龄女性患者卵巢功能有不同程度的损伤,重则致卵巢早衰,已成为卵巢早衰发病的一个重要原因。因此,改善和恢复患者卵巢功能,已成为一个重要的课题。 目的：探索骨髓间充质干细胞治疗化疗所致卵巢损伤的可行性及疗效。 方法：建立化疗性卵巢衰竭模型,建模后注射PKH26标记后的骨髓间充质干细胞,于移植细胞后第15,30,45,60天,各取5只大鼠经尾静脉取血测卵泡刺激素、雌二醇水平并处死大鼠,留取右侧卵巢行常规病理切片,光学显微镜下记录卵巢卵泡数量变化。细胞移植后30 d,取2只大鼠与雄鼠合笼,观察生殖能力的差异。 结果与结论：18%(4/22只)大鼠移植干细胞后动情周期能逐渐恢复,卵泡刺激素水平有所下降而雌二醇水平上升,卵巢病理切片提示卵泡数量减少,大鼠生育能力未受损害。结果表明骨髓间充质干细胞可以部分改善化疗后大鼠卵巢功能。 中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：     

淫羊藿联合骨髓间充质干细胞移植治疗肾病大鼠的作用

BACKGROUND: A large number of studies have shown that bone marrow mesenchymal stem cells (BMSCs) with differentiation ability can be used to treat kidney injury. Epimedium has good anti-inflammatory and immune restoration ability. OBJECTIVE:To observe the effect of epimedium with BMSC transplantation on rat’s kidney disease caused by adriamycin nephrosis. METHODS: Fifty rats were equivalently randomized to receive no treatment in control group, tail vein injection of normal saline plus intragastric injection of normal saline in model group, tail vein injection of BMSCs plus intragastric injection of epimedium decoction in combined group, tail vein injection of BMSCs in BMSCs group, and intragastric injection of epimedium decoction in epimedium group. Rat models of adriamycin nephrosis were made in the latter four groups through tail vein injection of doxorubicin hydrochloride, while rats in the control group were only given the same volume of normal saline. Twenty-four hours after modeling, rats in the latter four groups began to be given the corresponding treatments. Epimedium decoction was given in the combined and epimedium groups at a dose of 1 mL/d, for consecutive 1 week.  RESULTS AND CONCLUSION:Compared with the model group, mitigated kidney injury and reduced urinary protein level were found in rats undergoing BMSCs and/or epimedium decoction treatment; the levels of plasma albumin and catalase were significantly increased in the combined group and epimedium group, while the levels of serum cholesterol, triglycerides and malondialdehyde decreased; the mRNA expression of transforming growth factor β1 in the kidney tissues was dramatically declined in the combined group and BMSC group. To conclude, the combined use of epimedium and BMSCs is superior to their use alone in the treatment of kidney disease.     

骨髓间充质干细胞移植治疗系统性红斑狼疮

BACKGROUND: The non-specific immune suppression method is generally used for treatment of systemic lupus erythematosus, but poor prognosis, such as infection and high recurrence rate, exists. OBJECTIVE: To evaluate the therapeutic effect of bone marrow mesenchymal stem cell transplantation on systemic lupus erythematosus in mice. METHODS: Sixteen mice with systemic lupus erythematosus were equivalently randomized into control and experimental groups, or then subjected to passage 3 bone marrow mesenchymal stem cell transplantation or the equal volume of normal saline via the tail vein, respectively. Mouse urine samples were collected to detect urine protein levels by Bradford method. Blood samples from the tip of the mouse tail were extracted to detect serum anti-ds-DNS antibody concentration by radioimmunoassay. Mouse kidney tissues were taken and observed pathohistologically through hematoxylin-eosin staining and immunohistochemistry staining under microscope. Flow cytometry was used to detect the expression of CD4+CD25+T cells in the inner canthus blood, fresh spleen and thymus. RESULTS AND CONCLUSION: Within 10 weeks after cell transplantation, the urine protein levels in the two groups were gradually increased, and the rising velocity was higher in the control group than in the experimental group. From the 4th to 10th week, the urine protein levels in the experimental group were significantly lower than those in the control group (P < 0.05). In the control group, lymphocyte infiltration was visible in the kidney tissues with a few of plasmocytes, and pathological findings showed the mice presented with interstitial nephritis; in the experimental group, the mice had no pathological changes in the kidney. In the two groups, immune complexes were found in the mesangial area, which showed a patch-like distribution in the control group and a punctate distribution in the experimental group; the relative proportion of the occupied area in the experimental group was significantly lower than that in the control group. The expression level of CD4+CD25+T cells in the blood and thymus were significantly higher in the experimental group than the control group (P < 0.05), and the expression level of CD4+CD25+T cells in the spleen was slightly higher in the experimental group than the control group with no significant difference (P > 0.05). The serum anti-ds-DNA antibody concentration in the experimental group was significantly lower than that in the control group (P < 0.05). Taken together, bone marrow mesenchymal stem cell transplantation can improve the pathological damage in systemic lupus erythematosus mice, and has a certain therapeutic effect on systemic lupus erythematosus.       

骨髓间充质干细胞移植治疗颞下颌关节骨关节病

背景：随着干细胞技术的发展,利用组织工程技术修复软骨损伤成为一种可能,而骨髓间充质干细胞由于其优良的特性成为研究重点。 目的：通过体外培养骨髓间充质干细胞,注入兔颞下颌关节紊乱病动物模型,观察干细胞对兔颞下颌关节紊乱病的治疗效果。 方法：通过体外全血贴壁法培养骨髓间充质干细胞并进行鉴定;细胞在体外扩增,诱导成软骨细胞后待用。以Ⅱ型胶原酶进行颞下颌关节腔内注射,建立颞下颌关节紊乱病动物模型,关节腔内注射诱导后成软骨细胞设为实验组,对照组注射未进行诱导的细胞进行比较,通过观察动物咀嚼和组织切片观察治疗效果。 结果与结论：实验分离的细胞7-14 d可见少量集落形成,20 d时观察见细胞基本铺满瓶底。经stro-1⁺、CD44⁺流式细胞及免疫组化测定细胞表达间充质干细胞特性;细胞在诱导成软骨细胞后Ⅱ型胶原免疫组化染色强阳性。兔颞下颌关节注射胶原酶可在2周时出现偏侧咀嚼症状,骨髓间充质干细胞诱导的成软骨细胞关节腔注入动物模型后,实验组明显弱于对照组。组织切片显示诱导的成软骨细胞可促进关节损伤的修复,软骨及胶原生成多于对照组。说明骨髓间充质诱导的成软骨细胞关节腔注入后可促进兔颞下颌关节骨关节病愈合。     

骨髓间充质干细胞移植联合依达拉奉抑制脑梗死后的神经元凋亡

背景：国内外多项研究证实骨髓间充质干细胞移植对脑梗死组织具有一定的神经保护作用。依达拉奉是一种新型强效小分子羟自由基清除剂,可通过清除脑梗死产生的自由基,抑制神经细胞损伤,从而起到脑保护作用。 目的：观察骨髓间充质干细胞移植联合依达拉奉对大鼠脑梗死组织水通道蛋白4、Bcl-2、脑源性神经营养因子表达的影响。 方法：选取Wistar大鼠80只,建立右侧大脑中动脉闭塞模型,随机分为对照组、骨髓间充质干细胞组、依达拉奉组和联合治疗组。建模6 h后通过尾静脉分别注入移植液,对照组注射培养液,骨髓间充质干细胞组注射骨髓间充质干细胞,依达拉奉组给予依达拉奉注射液,联合组同时注入骨髓间充质干细胞和依达拉奉注射液。分别在伤后72 h将大鼠麻醉后断头取脑,应用RT-PCR、Western Blot法检测脑组织中水通道蛋白4、Bcl-2、脑源性神经营养因子基因表达和蛋白合成变化。伤后12,24,36 h取大鼠脑组织以TUNEL法测定细胞凋亡情况。 结果与结论：RT-PCR、Western Blot结果显示,在骨髓间充质干细胞与依达拉奉联合治疗组中,Bcl-2、脑源性神经营养因子的表达明显高于骨髓间充质干细胞组、依达拉奉组及对照组(P < 0.05);而水通道蛋白4的表达低于其余各组(P < 0.05)。TUNEL测定结果显示,联合治疗组中免疫组化呈棕色的凋亡细胞明显少于单独治疗组及对照组。提示骨髓间充质干细胞移植与依达拉奉联合应用治疗大鼠脑梗死,可进一步促进损伤局部脑源性神经营养因子及Bcl-2的表达,对神经细胞凋亡具有明显的抑制作用,同时可下调水通道蛋白4水平,减轻脑水肿程度,二者联合运用的效果明显优于单独治疗组。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：     

骨髓间充质干细胞移植联合氯化锂治疗兔股骨头缺血坏死疗效

BACKGROUND: Lithium chloride can promote the proliferation and osteogenic capacity of bone marrow mesenchymal stem cells in the necrotic region after avascular necrosis of the femoral head, which has become an issue of concern. OBJECTIVE: To compare the advantages and disadvantages of bone marrow stem cell transplantation combined with lithium chloride in the treatment of rabbit femoral head necrosis. METHODS: Passage 2 bone marrow mesenchymal stem cells from 1-week-old New Zealand rabbits were cultured in 0, 5, 10, 20, 40 mmol/L lithium chloride. Forty-eight healthy adult New Zealand rabbits were selected to make femoral head necrosis models in the right femoral head using liquid nitrogen freezing method and then randomized into four groups: model group with no implantation; lithium chloride group given lithium chloride treatment at 3 days after modeling; cell transplantation group given gelatin sponge implantation and bone marrow mesenchymal stem cell suspension injection into the femoral head after modeling; combined group given bone marrow mesenchymal stem cell suspension injection and lithium chloride treatment. Intraperitoneal injection of lithium chloride (45.2 mg/kg) was given daily beginning at the postoperative 3rd day, and the treatment duration was 4 weeks. RESULTS AND CONCLUSION: Lithium chloride at 10 mmol/L had the maximum effect on the proliferation of rabbit bone marrow mesenchymal stem cells, and if the concentration of lithium chloride was > 10 mmol/L, the promotion role of lithium chloride began to decline. After combined treatment, the morphology of the femoral head was restored a little, with increased bone density and thickened trabecular bone; the level of β-catenin in the femoral head was significantly increased in the combined group compared with the cell transplantation group or the lithium chloride group. These findings show that bone marrow stem cell transplantation combined with lithium chloride treatment can promote the recovery from femoral head necrosis by increasing bone mass of the trabecular bone and bone density of the femoral head in the necrotic region.      

骨髓间充质干细胞移植联合吡拉西坦修复大鼠脊髓损伤

背景：单纯的干细胞移植对脊髓损伤的修复作用并不理想,主要是因为脊髓损伤后损伤区域神经组织的水肿、缺血、缺氧等引起继发性损伤造成的。 目的：在骨髓间充质干细胞移植治疗大鼠脊髓损伤的同时应用吡拉西坦,观察两者对大鼠脊髓损伤恢复的影响。 方法：雌性Wistar大鼠参照改良Allen打击法制备大鼠脊髓损伤模型。随机分成3组,即单纯损伤组、骨髓间充质干细胞移植组及骨髓间充质干细胞移植联合吡拉西坦组。于伤后1,2,4,6,8周进行BBB评分和斜板实验等运动功能检测。第4周取材行病理切片苏木精-伊红染色,通过SRY-PCR检测雄性大鼠Y染色体上特有的基因SRY,从而得知移植骨髓间充质干细胞是否存活。8周后取材,行辣根过氧化物酶示踪观察,并通过透射电镜观察轴突的再生情况。 结果与结论：伤后4周,骨髓间充质干细胞移植组、联合治疗组大鼠后肢运动功能均有较明显恢复,联合治疗组较骨髓间充质干细胞移植组恢复快(P < 0.05)。单纯损伤组亦有所恢复,但程度较轻。病理切片单纯损伤组未见神经轴索通过;骨髓间充质干细胞移植组可见少量神经轴索样结构;联合治疗组可见较多神经轴索样结构。骨髓间充质干细胞移植组、联合治疗组有SRY基因表达,单纯损伤组未检测到SRY基因。辣根过氧化物酶阳性神经纤维数联合治疗组﹥骨髓间充质干细胞移植组>单纯损伤组,差异具有显著性意义(P < 0.05)。透射电镜下,骨髓间充质干细胞移植组、联合治疗组正中横断面可见新生的无髓及有髓神经纤维。提示骨髓间充质干细胞移植联合吡拉西坦促进大鼠损伤脊髓结构和功能恢复的效果明显优于单纯细胞移植组,两者联用具有协同效应。     

灯盏细辛联合骨髓间充质干细胞移植对脑梗死的保护作用机制

背景：通过细胞移植重建损伤脑组织成为治疗脑梗死的新途径,骨髓间充质干细胞成为近年来细胞移植治疗领域的重要种子细胞之一。 目的：探讨灯盏细辛注射液联合骨髓间充质干细胞移植对急性脑梗死大鼠S100B蛋白及超氧化物歧化酶表达的影响。 方法：采用线栓法制作大鼠急性脑梗死模型,建模成功后将80只SD大鼠随机分为对照组、灯盏细辛组、骨髓间充质干细胞组及联合组。分别于治疗前后用酶联免疫法检测各组大鼠血清S100B蛋白水平,黄嘌呤氧化酶法检测各组大鼠血清超氧化物歧化酶的表达;通过NIHSS神经功能评分观察模型大鼠的神经行为学变化,通过TTC染色测定脑梗死体积。 结果与结论：在治疗后第3,7,14天,灯盏细辛组、骨髓间充质干细胞组的S100B蛋白水平明显低于对照组,但高于联合组,差异有显著性意义(P < 0.05);灯盏细辛组、骨髓间充质干细胞的超氧化物歧化酶表达水平明显高于对照组,低于联合组,差异有显著性意义(P < 0.05);治疗后第1,2,3周各组的NIHSS神经功能评分比较,联合组<灯盏细辛组及骨髓间充质干细胞组<对照组,差异有显著性意义(P < 0.05);在治疗后2周联合组的脑梗死体积明显小于灯盏细辛组及骨髓间充质干细胞组,灯盏细辛组及骨髓间充质干细胞组又明显小于对照组,差异有显著性意义(P < 0.05)。结果表明灯盏细辛注射液联合骨髓间充质干细胞移植能够抑制急性脑梗死大鼠S100B蛋白表达,提高超氧化物歧化酶活性,从而起到脑保护作用。 中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程     

骨髓间充质干细胞移植治疗肺气肿：静脉和气管途径谁更有优势？

背景：骨髓间充质干细胞移植可以抑制肺气肿炎症反应及细胞凋亡,治疗肺功能严重损害已经得到实验证实。目的：探讨不同途径移植骨髓间充质干细胞对实验性肺气肿炎症反应及细胞凋亡的抑制作用。方法：Wistar雌性大鼠随机分对照组、静脉组及气管组,均用烟熏加气管内滴注猪胰弹性蛋白酶法建立肺气肿模型。静脉组和气管组经尾静脉及气管途径移植雄性大鼠骨髓间充质干细胞,对照组尾静脉和气管内注射PBS,移植后14 d观察大鼠肺组织病理学变化,检测肺泡壁细胞凋亡指数,支气管肺泡灌洗液肿瘤坏死因子α水平。结果与结论：与对照组相比,静脉组及气管组均能减轻肺气肿病理改变,降低肿瘤坏死因子α水平及凋亡指数,差异有显著性意义(P < 0.01);但是静脉组与气管组比较,肺气肿病理改变、肿瘤坏死因子α水平及凋亡指数差异无显著性意义(P > 0.05)。结果表明两种途径移植骨髓间充质干细胞对肺气肿均有明显的治疗作用,从方便易行的角度考虑经静脉移植骨髓间充质干细胞治疗肺气肿较为有优势。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：