fascin-1与E-cadherin在皮肤鳞状细胞癌中表达的相关性研究

目的:探讨fascin-1与E-cadherin在皮肤鳞状细胞癌中的表达及两者之间的相关性.方法:应用fascin-1、E-cadherin单克隆抗体检测24例皮肤鳞状细胞癌中fascin-1和E-cadherin的表达情况.结果:fascin-1表达与组织病理学分级、P53表达、Ki-67表达有关(P＜0.05),而与年龄、性别无关(P＞0.05);fascin-1与E-cadherin的表达呈负相关(r=-0.63,P＜0.05).结论:在皮肤鳞状细胞癌中.fascin-1表达上调与E-cadherin表达缺失可能与肿瘤的侵袭及转移相关.     

皮肤鳞状细胞癌

皮肤鳞状细胞癌的发病率仅次于基底细胞癌,为最常见的皮肤癌之一。近年来其发病率在许多国家不断增加。该文较详细介绍了关于鳞状细胞癌的流行病学、病因学、临床特征、诊断和治疗的研究近况。     

热休克蛋白72在皮肤鳞状细胞癌中的表达

应用免疫组织化学（ＳＡＢＣ法） 和核酸原位杂交技术检测了正常人皮肤、皮肤鳞状细胞癌中热休克蛋白７２ 的表达情况。结果显示,ＨＳＰ７２ 在正常皮肤的表皮层及毛囊外根鞘即有构成性表达, 可能是一种自身保护功能。ＨＳＰ７２ 在皮肤鳞癌中高表达, 可能通过影响细胞生长调控而在肿瘤发生、发展过程中具有重要作用。     

皮肤结核继发鳞状细胞癌

报告1例额部皮肤结核后并发带状疱疹及鳞状细胞癌(简称鳞癌).患者男,56岁.额部患皮肤结核20余年,2年前在皮肤结核的皮损处出现带状疱疹.通过正规的抗结核和病毒治疗后,皮损逐渐好转.大约1年前红斑处外伤后渐有两枚肿物长出,较大的肿物经手术切除而治愈,经组织病理证实为鳞癌,2个多月后,另一个肿物迅速生长,再次住院彻底切除肿物,术后组织病理也显示为鳞癌,随访至今未见复发.提示须注意长期皮肤结核的并发症,组织病理学检查非常重要.     

皮肤鳞状细胞癌CD44v6蛋白表达

采用免疫组织化学及图像分析技术对66例皮肤鳞状细胞癌、12例淋巴结转移灶、11例假性上皮瘤样增生、10例正常皮肤进行检测，探讨CD44v6与皮肤鳞状细胞癌的发生、发展与转移的关系。结果表明鳞状细胞癌CD44v6表达下调，且分化越差下调越明显，与是否转移及发病部位无关。提示CD44v6对维持表皮正常结构起重要作用，并与细胞增殖分化程度 有关，可以作为一个研究皮肤鳞状细胞癌发生发展的分子标志。     

热休克蛋白27在皮肤基底细胞癌和鳞状细胞癌中的表达

热休克蛋白(heat shock proteins,HSP)是细胞在应激状态下产生的一组高度保守的蛋白质家族中的成员,主要在蛋白质合成、折叠、装配、退化、信号转导等方面发挥调节作用.HSP27在调节细胞周期,参与细胞增殖和分化方面的证据也越来越多地被人们发现.为了探讨HSP27在表皮中的表达及其与皮肤肿瘤中细胞增殖和细胞分化的关系,我们采用免疫组化法检测HSP27、增殖细胞核抗原(PCNA)在皮肤基底细胞癌、鳞状细胞癌中的表达.     

人皮肤鳞状细胞癌组织大电导钙激活钾通道蛋白的表达

关于肿瘤细胞的病理生理研究中发现,离子通道具有调控细胞生长、分化和增殖等重要作用,它与细胞电生理、细胞内信号传递、基因表达等基本生命现象密切相关,其功能、结构或数量等的异常是导致恶性肿瘤等重大疾病发生发展的决定性因素之一[1-2].     

Syndecan-1蛋白在皮肤鳞状细胞癌的表达及意义

目的 检测皮肤鳞状细胞癌（鳞癌）患者血清可溶性多配体蛋白聚糖1（SDC1）水平和SDC1蛋白在组织中的表达及两者的相关性。 方法 免疫组化方法检测93例鳞癌及30例健康对照者表皮SDC1的表达,酶联免疫吸附测定（ELISA）检测81例鳞癌患者和30例健康对照者血清中可溶性SDC1的表达水平。 结果 鳞癌组织中,SDC1表达明显低于健康对照组（Z = 3.85,P < 0.01）。在不同肿瘤厚度和分化程度的鳞癌中,SDC1表达强度随肿瘤厚度的增加和分化程度地降低而有下降趋势（χ2分别为11.66和12.51,均P < 0.01）。鳞癌患者中伴淋巴结转移组SDC1表达强度显著低于无淋巴结转移组（Z = 2.20,P < 0.05）。鳞癌患者血清可溶性SDC1表达水平显著高于健康对照组（Z = 4.12,P < 0.01）,且随着肿瘤厚度的增加和临床分期的变晚血清可溶性SDC1水平逐渐升高。侵袭性鳞癌的SDC1水平高于原位鳞癌的患者（Z = 3.02,P < 0.01）,但不同分化程度的侵袭性鳞癌血清SDC1水平比较,差异无统计学意义（均P > 0.05）。有淋巴结转移的鳞癌患者血清SDC1水平明显高于无淋巴结转移组（Z = 5.30,P < 0.01）。鳞癌患者血清中可溶性SDC1的水平与组织中SDC1表达强度呈负相关（rs = -0.625,P < 0.01）。用受试者工作曲线法判断血清可溶性SDC1水平对诊断有无淋巴结转移的最佳临界点为65.5 μg/L,其敏感度为73.7%,特异度为87.1%,曲线下面积值为0.904（0.840 ~ 0.968）。 结论 鳞癌组织中,SDC1表达减弱和血清中可溶性SDC1水平升高与鳞癌的侵袭性相关,血清SDC1水平升高对鳞癌患者淋巴结转移的诊断具有一定的价值。     

皮肤鳞状细胞癌

皮肤鳞状细胞癌的发病率仅次于基底细胞癌，为最常见的皮肤癌之一，近年来其发病率在许多国家不断增加，该文较详细介绍了关于鳞状细胞癌的流行病学，病因学、临床特征、诊断和治疗的研究近况。     

CK7 expression in primary cutaneous squamous cell carcinoma

Aim: To evaluate cytokeratin 7 (CK7) immunoreactivity in invasive primary cutaneous squamous cell carcinomas (SCCs). Methods: Twenty‐seven primary cutaneous SCCs from 25 patients were evaluated for tumor grade using hematoxylin and eosin‐stained slides and for percentage and intensity of immunoreactivity for CK7. All cases exhibited features of SCC with an in situ component. No glandular or tubular differentiation was present. Staining intensity was graded on a scale of 0–3, with 0 indicating no reaction. Of immunoreactive cases, percentage of tumor staining and distribution of immunoreactivity was documented. Results: Six of 27 SCCs (22%) exhibited immunostaining for CK7. Of those cases, three were poorly differentiated, exhibiting 2 to 3+ intensity in 5–15% of cells. Two were poorly differentiated, with 2 to 3+ intensity in 30–60% of cells. The remaining immunoreactive tumor was moderately differentiated, with 1+ intensity and 5% staining in an area of microinvasion. Conclusion: A subset of cutaneous SCCs, in particular, poorly differentiated tumors, may show focal‐to‐partial immunoreactivity for CK7. This is important to bear in mind when immunohistochemistry is used to distinguish SCC from simulants, such as porocarcinoma, or other adnexal carcinomas with squamous metaplasia. Pulitzer M, Desman G, Busam KJ. CK7 expression in primary cutaneous squamous cell carcinoma.     

CD147 expression in advanced cutaneous squamous cell carcinoma

Background: CD147 is upregulated in multiple cancer types, but its expression in advanced cutaneous squamous cell carcinoma (SCC) is unknown. Our purpose was to evaluate the expression patterns of CD147 and related monocarboxylate transporters (MCT1, MCT4) to determine their correlation with survival. Methods: This is a retrospective cohort study of patients with advanced stage cutaneous SCC of the head and neck who presented to a tertiary care center between 1998 and 2006 (n=50). CD147, MCT1 and MCT4 expression levels were assessed using immunofluorescence analysis of archived tumor samples and correlated with survival and clinicopathologic characteristics. Results: The majority of patients (92%, n = 46) were diagnosed with stage III disease, with 46% (n = 23) having positive regional lymph node metastasis and 8% (n = 4) with distant metastasis. Primary malignancies had an overexpression of CD147 (78%; n = 35), MCT1 (23%; n = 10) and MCT4 (47%; n = 20). In addition, there was a significant relationship between the overexpression of CD147 and node positive disease (p = 0.048). Two‐ and five‐year survival rates were 69 and 61%, respectively. There was a trend toward decreased survival in patients with overexpression of CD147 (p = 0.17), MCT1 (p = 0.11) and MCT4 (p = 0.15). Conclusion: CD147 may represent a biomarker or potential therapeutic target in advanced cutaneous SCC. Sweeny L. Dean NR, Frederick JW, Scott Magnuson J, Carroll WR, Desmond RA, Rosenthal EL. CD147 expression in advanced cutaneous squamous cell carcinoma.     

PD-L1在皮肤鳞状细胞癌及角化棘皮瘤中的表达

目的：检测PD-L1在皮肤鳞状细胞癌（cSCC）及角化棘皮瘤（KA）中的表达,分析PD-L1与cSCC分化程度的相关性。方法：免疫组化染色检测PD-L1在cSCC及KA中表达水平。结果：共检测56例cSCC患者和32例KA患者标本，PD-L1在cSCC组和KA组中的阳性率分别为66.07%和62.50%，均显著高于正常对照组（9.38%）（Ps＜0.01）。PD-L1阳性率在cSCC与KA组的差异没有统计学意义（P＞0.05）。PD-L1的表达强度与cSCC的分化程度呈负相关（P＜0.05）。结论：PD-L1不能作为区分cSCC与KA的指标。PD-L1的表达强度与cSCC分化程度呈负相关。     

皮肤鳞状细胞癌的研究进展

皮肤鳞状细胞癌(CSCC)是世界范围内最常见的非黑色素瘤皮肤癌之一。研究显示,近年来CSCC的发病率呈不断增高的趋势,其临床表现多样,具有侵袭性,可通过淋巴、血液途径进行转移,晚期患者具有较高的复发率和致死率,对生命造成极大的威胁。本文结合近几年国内外有关CSCC的研究进展进行综述,以期对CSCC的早期诊断、治疗选择和预防提供帮助。     

Syndecan-1 expression is diminished in acantholytic cutaneous squamous cell carcinoma

Syndecan-1 is a cell surface proteoglycan predominantly expressed on the surface of adult epithelial cells, and is normally present in all epidermal layers except for the most superficial terminally differentiated cells. Syndecan-1 mediates cell-cell and cell-extracellular matrix adhesion, thereby influencing cell morphology and growth characteristics. In addition, in vitro studies have shown that expression of syndecan-1 on tumor cells inhibits their invasion into the extracellular matrix. A total of 23 cutaneous biopsies of squamous cell carcinoma, including acantholytic squamous cell carcinoma, invasive squamous cell carcinoma which was not acantholytic, and squamous cell carcinoma in situ were examined for syndecan-1 immunoreactivity. The level of syndecan-1 expression was related to the degree of squamous cell dyshesion, with expression being greatest in the in situ lesions and least in the acantholytic lesions. The loss of syndecan-1 expression with increasing dyshesion of squamous cell carcinoma may be a mechanism for loosening of intercellular and cell-extracellular matrix attachments, thereby promoting the invasion of neoplastic cells into the dermis.