甲硝唑凝胶在治疗侵袭性牙周炎中的临床观察

目的观察牙周袋内使用甲硝唑凝胶治疗侵袭性牙周炎的临床效果。方法侵袭性牙周炎患者56例（256颗牙）,随机分成2组,均采用龈上洁治、龈下刮治术,治疗组采用甲硝唑凝胶牙周袋内注射治疗（注满约10mg）,每周1次,共4次;对照组口服罗红霉素片（每日2次,每次1片0.15g）和甲硝唑片（每日3次,每次1片0.1g）,每周连续服用3日,共4周。记录并分析治疗前后2周、停药2周、停药4周后的出血指数（BI）、牙体松动度、牙周袋探诊深度（PD）和牙体附着水平（CAL）。结果治疗组和对照组在治疗前牙周水平基线一致,治疗后,治疗组BI、PD、CAL均较对照组明显改善（P〈0.05）。结论牙周袋内局部应用甲硝唑凝胶能提高侵袭性牙周炎的治疗效果,是值得临床广泛应用的局部治疗侵袭性牙周炎的药物。     

Er，Cr：YSGG激光在慢性牙周炎基础治疗中的应用观察

目的：评价Er,Cr：YSGG牙周袋内清创的临床效果。方法：纳入19名慢性牙周炎患者,采用左右半口对照研究,随机分为A组：Er,Cr：YSGG激光牙周袋内清创;B组：手工龈下刮治器械牙周袋内清刨。比较A、B组治疗后6、12周PD、BOP、CAL的变化及治疗疼痛程度VAS值。结果：基线PD≥4mm的位点治疗后6周和12周PD、CAL和BOP与基线相比,均有非常明显下降（P〈0．01）。且基线时PD≥6mm的位点,治疗后12周激光组PD降低显著高于对照组。激光组的VAS值明显低于手工器械组（P〈0．01）。结论：Er,Cr：YSGG激光对慢性牙周炎患者进行牙周袋内壁清创是安全有效的。     

牙周内窥镜辅助超声龈下刮治对残留牙周袋临床疗效的评价研究

目的 评价牙周内窥镜下超声龈下刮治对牙周基础治疗后残留牙周袋的临床治疗效果.方法 收集20例慢性牙周炎患者259颗经牙周基础治疗后仍残留深牙周袋的患牙,行牙周内窥镜下超声龈下刮治,比较治疗前及治疗后3个月全口牙周探诊出血(BOP)和牙周探诊深度(PD)的变化.结果 内窥镜治疗后3个月全口平均PD值和BOP位点百分比有显著改善(P<0.001).单根牙和多根牙的平均PD均显著降低(P<0.001),PD≥5mm位点百分比显著改善(P<0.05),其中重度牙周袋位点的改善更明显(P<0.05).根分叉病变位点百分比治疗前后无明显变化.结论 残留牙周深袋经牙周内窥镜辅助超声龈下刮治后,单根牙和多根牙都有显著治疗效果,并且单根牙重度牙周袋位点改善更明显,但是多根牙II度及以上根分叉病变位点的改善有限.     

甲硝唑棒在深牙周袋病损治疗中的作用

目的：评价牙康（甲硝唑棒）治疗深牙周袋的作用。方法：牙康＋刮治疗7名牙周炎患者的7个深牙周袋位点,单纯刮治另7个位点为对照。治疗后7天、28天观察龈下微生物变化及临床疗效。结果：牙康治疗后龈下总细菌数及能动菌、梭形杆菌、螺旋体百分比显著降低,7天时显著低于对照,配合刮治28天时探深显著代于治疗前;而单纯刮治后探深无显著降低。结论：牙康＋刮治治疗深牙周袋,能快而有效地抑制龈下致病微生物,临床效果更佳。     

甲硝唑棒在深牙周袋病损治疗中的作用

目的 :评价牙康 (甲硝唑棒 )治疗深牙周袋的作用。方法 :牙康 +刮治治疗 7名牙周炎患者的 7个深牙周袋位点 ,单纯刮治另 7个位点为对照。治疗后 7天、2 8天观察龈下微生物变化及临床疗效。结果 :牙康治疗后龈下总细菌数及能动菌、梭形杆菌、螺旋体百分比显著降低 ,7天时显著低于对照 ,配合刮治 2 8天时探深显著低于治疗前 ;而单纯刮治后探深无显著降低。结论 :牙康 +刮治治疗深牙周袋 ,能快而有效地抑制龈下致病微生物 ,临床效果更佳。     

盐酸米诺环素软膏治疗逆行性牙髓炎的临床评价

目的 :评价牙周袋内局部使用盐酸米诺环素软膏 ,辅助治疗逆行性牙髓炎的临床效果。方法 :选择逆行性牙髓炎 3 2例 ,随机分为实验组和对照组 ,均无原发牙体牙髓病。患牙有深达根尖区的牙周袋 (探诊深度≥ 5mm)。实验组 76个牙位点 ,对照组 84个牙位点。两组均同时进行牙髓治疗和牙周治疗。在龈上洁治和龈下刮治术后 ,实验组牙周袋内注入盐酸米诺环素软膏 ,对照组牙周袋内只用生理盐水冲洗 ,每周 1次 ,共 3次。在基线、治疗后第 4周、第 8周时 ,记录菌斑指数 (PLI)、探诊出血 (BOP)、牙周袋深度 (PD)、临床附着丧失 (CAL)等指标。结果 :治疗后第 4周和第 8周 ,两组各项指标测定值与基线相比均有明显改善 ,两次复查时实验组各指标的改善均明显优于对照组 ,差异有显著性 (P <0 .0 1)。结论 :牙周袋局部应用盐酸米诺环素软膏辅助治疗逆行性牙髓炎 ,能够获得较好的临床效果。     

四环素透明质酸凝胶辅助治疗慢性牙周炎的疗效观察

目的：观察四环素透明质酸凝胶辅助治疗牙周炎的临床疗效。方法：采用口内自身对照研究方法，选择68名牙周炎病人共200个牙周袋分为四环素透明质酸凝胶实验组和对照组。实验组患牙行洁治、刮治、根面平整后，龈下辅助给予四环素透明质酸凝胶，对照组仅作洁治、刮治、根面平整，观察两组患牙的临床指标（PLI、SBI、PD、AL、MD）和龈下菌斑螺旋体构成比的动态变化。结果：两组患牙各临床指标在治疗后均显著改善，治疗后4周，对照组PD、AL、MD及龈下菌斑螺旋体的构成比与治疗后1周相比无显著差异。结论：慢性牙周炎在基础治疗上，龈下局部应用四环素透明质酸凝胶辅助治疗牙周炎可取得较好的疗效。     

超声龈下刮治同步药物冲洗对龈下微生物的影响

目的 :比较Master 40 0超声龈下刮治同步药物冲洗系统与传统超声龈下刮治 +药物冲洗对龈下微生物的影响及临床疗效。方法 :选取慢性成人牙周炎患牙 2 0个 ,随机分入实验组 (超声龈下刮治同步药物冲洗 )和对照组 (传统超声龈下刮治 +药物冲洗 )。记录治疗前、后 0、7、14d的探诊出血 (BOP)、探诊深度 (PD) ,并采集龈下菌斑样本培养 ,观察比较总菌落形成单位 (CFU)和牙周可疑致病菌检出率及检出量的变化。结果 :治疗后两组PD、BOP、CFU和牙周可疑致病菌数量均显著减少 (P <0 .0 5 ) ;实验组牙龈卟啉单胞菌 (P <0 .0 1)和二氧化碳噬纤维菌 (P <0 .0 5 )明显少于对照组。结论 :Master 40 0超声龈下刮治同步药物冲洗系统比传统超声龈下刮治 +药物冲洗能更有效地减少龈下致病微生物的数量     

重度广泛型侵袭性牙周炎患者牙周序列治疗的临床研究

目的：观察重度广泛型侵袭性牙周炎（generalized aggressive periodontitis, GAgP）患者牙周序列治疗后临床指标的变化,并评价其治疗效果。方法：15例GAgP患者在龈上洁治后1个月内完成龈下刮治、根面平整及光动力治疗,刮治后4周再评估,行翻瓣及翻瓣植骨术,每3个月进行1次牙周维护,序列治疗后纵向观察12个月。在基线和维护期各时间点记录全口探诊深度（probing depth,PD）、探诊出血（bleeding on probing, BOP）和牙齿松动度,磨牙记录根分叉病变的程度。结果：在基线时患者平均PD（3．96±2．21）mm,BOP阳性位点占88．1％。序列治疗后12个月平均PD（2．36±1．28）mm,BOP阳性位点减少至8．7％,其中轻、中、重度位点治疗后PD值与治疗前比较差异有统计学意义（P〈0．05）。结论：GAgP患者经牙周序列治疗和定期牙周维护能取得良好的治疗效果。     

超声和手工龈下刮治对龈下微生物影响的研究

目的 :比较Perioproline超声龈下刮治和手工龈下刮治对龈下微生物的影响 ,评价Periopro line超声龈下刮治系统的临床疗效和工作效率。方法 :选取慢性成人牙周炎患者 7名 ,共 2 0个左右基本对称牙被纳入研究 ,随机分入实验组 (超声组 )和对照组 (手工组 )。记录治疗时间和治疗前、治疗后 0d、7d和 14d的PD ,BOP值 ,及采集龈下菌斑样本培养鉴定 ,观察比较总菌落形成单位 (CFU)和牙周可疑致病菌检出率及检出量的变化。结果 :两组在治疗后PD、BOP值及龈下菌斑总量 (CFU)和牙周可疑致病菌数量及检出率均显著下降 (P <0 .0 5) ;治疗前后各时段组间均无明显差别 ;超声组的治疗时间明显少于手工组 (P <0 .0 5)。结论 :Perioproline超声龈下刮治和手工龈下刮治均可有效减少龈下微生物的量 ,改善患牙的牙周临床状况 ,且两者间无差异 ,但Perioproline超声龈下刮治明显较手工刮治节省时间     

Topical metronidazole application compared with subgingival scaling

Abstract The aim of this study was to compare the topical application of a metronidazole 25% dental gel with subgingival scaling. 30 patients from the recall program participated in this open randomised study with split-mouth design. Pocket probing depths (PPD) and bleeding on probing (BOP) were measured before and 2, 12 and 24 weeks after the end of the treatment period. In addition, subgingival plaque samples were taken from all mesial sites and analysed with dark-field microscopy. All patients had at least 1 tooth in each quadrant with a PPD of 5 mm or more that should bleeding on probing, when entering the study. The treatment consisted of 2 applications of the dental gel in 2 randomly selected quadrants (on days 0 and 7) as well as simultaneous subgingival scaling of the remaining quadrants. Oral hygiene instruction was given on day 21. The average PPD and the average frequency of BOP were calculated for all sites with an inital PPD of 5 mm or more and continued at each examination, using the same sites. The statistical analyses showed that both treatments were effective in reducing PPD and BOP over the 6-month period. At the end of the follow-up period, the mean reduction in PPD was 1.3 mm after gel treatment and 1.5 mm after subgingival scaling. BOP was reduced by 35% and 42%, respectively. No significant differences between the 2 treatments were detected. Dark-field microscopy showed a shift towards a more healthy microflora for both treatment modalities; this persisted throughout the 6-month period. Application of a 25% metronidazole dental gel on recall patients seems to be as effective on the investigated clinical and microbiological parameters as subgingival scaling.     

Topical metronidazole application in recall patients

Abstract The aim of this randomised study in split-mouth design, in which 24 patients from the recall programme of Marburg University Department of Period-ontology were enrolled, was to compare topical application of a metronidazole 25% dental gel with subgingival scaling. The clinical parameters used were pocket probing depth (PPD) and bleeding on probing (BOP); these were recorded at baseline and 1, 3, 6, 14, 18 and 24 months after completion of treatment. In addition, plaque samples were taken from all mesial pockets for evaluation by dark-field microscopy. All patients had at least one tooth in each quadrant with a PPD of 5 mm or more showing BOP when they entered the study. The treatment consisted of 2 applications of dental gel in 1 randomly selected quadrants (on days 0 and 7) as well as subgingival scaling of the remaining quadrants. Statistical evaluation of all sites with a baseline PPD of 5mm or more showed that both methods led to a significant reduction in PPD and BOP in the first 6 montKs. The average reduction in PPD was 1, 3 mm in the gel group and 1, 5 mm in the scaling group, with the tendency to bleeding being reduced by ca. 50% in both groups. After 24 months, improvements of 0, 6 mm and 0.5 mm respectively were observed in PPD, The tendency to bleeding had undergotie a slight increase but was still below baseline values. Mo statistically significant differences were observed between the two methods, although the results recorded with subgingival scaling were slightly better. Dark-field microscopy revealed a shift in the composition of the bacterial flora, suggesting a more physiological situation, which was maintained for 6 months before reverting consistently after 1 8 months to the baseline values of the plaque composition. Overall, application of a metronidazole 25% dental gel led m recall patients to an improvement in the investigated clinical and microbiological parameters comparable with subgingival scaling. After 24 months, the clinical parameters still displayed a very slight improvement over baseline values; after 18 months the microflora had reverted to its baseline composition.     

Repeated oral hygiene instructions alone, or in combination with metronidazole dental gel with or without subgingival scaling in adult periodontitis patients: a one-year clinical study

The aims of the present study were to evaluate the clinical efficacy of, and to monitor microbiologically, repeated oral hygiene instructions alone or in combination with metronidazole 25% gel or subgingival scaling with or without metronidazole gel in treatment of new, residual or recurrent periodontal pockets in previously treated adult periodontitis patients. Ten suitable patients were included in this randomized single-blind clinical study with an intra-individual design. Clinical parameters were measured before and at 1, 3, 6, 9 and 12 months after treatment. Subgingival plaque samples were taken at every examination from one selected site in each quadrant. Smears from plaque samples were silver-stained and differential counting was performed under light microscopy at 1000X magnification. Four treatment modalities: (i) oral hygiene instruction (OHI) alone; (ii) OHI and metronidazole dental gel; (iii) OHI and subgingival scaling; (iv) OHI and subgingival scaling plus metronidazole gel, were randomly assigned to one quadrant of each patient. At the 12-month examination, the mean reductions in probing pocket depth were 2.6 mm after OHI alone, 2.8 mm after OHI and metronidazole gel, 3.3 mm after OHI and subgingival scaling and 2.6 mm after oral OHI and subgingival scaling plus metronidazole gel. The mean gains in probing attachment level were 2.2 mm, 1.9 mm, 2.7 mm and 1.6 mm, respectively. Although there were statistically significant differences in treatment responses between some treatment modalities at some time points, these were not considered to be clinically significant. Differential counts showed a shift towards a healthy microflora in response to all treatment modalities. From the 12-month results, it was concluded that the metronidazole 25% gel produced positive effects on the clinical parameters and on the subgingival plaque microbiological composition in new, residual or recurrent pockets in previously treated chronic periodontitis patients. However, the metronidazole gel alone or in combination with scaling does not seem to have any additional clinically significant therapeutic effects over and above those derived from improved oral hygiene resulting from monthly recalls, with or without subgingival scaling.     

Topical metronidazole application as an adjunct to scaling and root planing

AIM: This study was carried out to investigate the effect of 2 applications of a metronidazole 25% dental gel as adjunctive therapy to subgingival scaling with root planing. METHOD: 59 of the original collective of 64 patients with adult periodontitis were observed for a 9-month period. This randomised single-blind study was carried out in split-mouth design. Each patient had to have at least 2 pockets with a probing depth of > or =5 mm in each quadrant. The clinical parameters, pocket probing depth (PPD), attachment level (AL) and bleeding on probing (BOP), were recorded at all teeth on days 0, 91, 175 and 259; in addition, subgingival plaque samples taken from 45 patients were analysed by means of dark-field microscopy. Therapy comprised subgingival scaling and root planing (SRP) of all quadrants and additional application of metronidazole 25%, dental gel in 2 randomly selected quadrants (SRP+Metro). Treatment was confined to teeth with a baseline PPD of > or =5 mm. Average PPD and AL and the incidence of BOP were computed for all pockets with a baseline PPD of > or =5 mm, and the 2 methods compared. The main efficacy variable for evaluation of the 2 treatments was the difference in PPD on day 259. RESULTS: Comparison of the 2 treatments revealed a statistically significant improvement in the clinical parameters for both treatment methods over the study period. Between baseline and day 259, significant differences in PPD (SRP+Metro: from 6.00 to 4.63 mm, SRP: from 6.02 to 4.83 mm) and BOP (SRP+Metro: from 67 to 31%, SRP: from 64 to 36%) were observed between the 2 treatment groups. Evaluation according to different patient groups demonstrated significant advantages of the combined therapy in previously-untreated patients, especially in female probands. Dark-field microscopy revealed a shift in the bacterial flora towards "healthy conditions". CONCLUSIONS: The results show that only minor advantages are to be gained from the application of a metronidazole 25% dental gel as adjunctive therapy to subgingival scaling. The distinctly better results of combined therapy in previously-untreated patients calls for more thorough investigation.     

Clinical responses to subgingival application of a metronidazole 25% gel compared to the effect of subgingival scaling in adult periodontitis

Abstract. A newly developed metronidazole 25% dental gel was compared with subgingival scaling in the treatment of adult periodontitis. 206 patients in 9 centres participated in the study. Probing pocket depth (PPD) and bleeding on probing (BOP) were recorded before treatment and 2, 6, 12, 18, and 24 weeks after the treatment. All patients had at least I tooth in each quadrant with a PPD of 5 mm or more. The treatments consisted of 2 applications of dental gel (days 0 and 7) in 2 randomly selected quadrants (split mouth design) and 2 sessions of subgingival scaling (1 quadrant on day 0, and 1 quadrant on day 7). Instruction in oral hygiene was given 2 weeks after completed treatment. The average PPD and the average frequency of BOP were calculated over all sites with initial PPD of 5 mm or more. PPD and BOP were thus, at each examination, calculated from the same sites. The mean PPD was 5.9 mm before gel application and 5.8 mm before scaling (p= 0.31). BOP was 88% in both treatment groups. 24 weeks after the treatment. PPD and BOP were significantly reduced in both groups and for both parameters (p < 0.01). PPD was reduced by 1.3 mm after gel application and 1.5 mm after scaling; BOP was reduced by 32% and 39%, respectively. The difference between the treatments was statistically significant, but considered as clinically unimportant.     

Clinical outcome observed in subjects with recurrent periodontal disease following local treatment with 25% metronidazole gel

BACKGROUND: The aim of this study was to evaluate the clinical outcome in patients with recurrent periodontal disease following treatment with 25% metronidazole gel. METHODS: Twenty subjects in a maintenance care program but with recurrent periodontal disease participated. Three months after scaling and root planing, a total of 40 sites, 2 in each patient, with probing depth > or = 5 mm were selected. One site randomly selected was treated with metronidazole gel (test) and the other site with a placebo gel (control). Baseline and follow-up measurements included plaque index (PI), gingival index (GI), bleeding on probing (BOP), probing depth (PD), and clinical attachment level (CAL). RESULTS: There were no statistically significant differences in PI, GI, BOP, PD, or CAL between test and control sites. CONCLUSION: This study showed that local treatment with 25% metronidazole gel did not seem to influence the clinical healing in this group of subjects with recurrent periodontal disease.     

3 regimens of topical metronidazole compared with subgingival scaling on periodontal pathology in adults

Abstract. This report presents the findings from an open randomized multicentre clinical trial designed to compare the clinical efficacy of scaling with application of 3 different preparations/dose frequencies of topical metronidazole in the treatment of adult periodontitis. The 4 treatments were: (A) metronidazole 25% dental gel administered 1 × a week for 2 weeks; (B) metronidazole 15% dental gel applied 1 × a week for 2 weeks; (C) metronidazole 15% dental gel applied 2 × a week for 2 weeks; (D) subgingival scaling, performed 1 × only. A split mouth design was used. Patients were included in the study if they had at least 1 tooth in each quadrant with a pocket depth ≥ 5 mm in at least 1 of 4 sites. A total of 61 patients from 4 centres were entered into the study. The efficacy parameters were probing pocket depth and bleeding on probing. Follow-up visits for recording of clinical efficacy were made at 2, 4, 6 and 12 weeks after the end of metronidazole treatment. All 3 antibiotic treatments (A, B, C) reduced the symptoms of periodontal pathology and yielded results comparable to those seen after subgingival scaling (D). When using a topical drug therapy, it seems important to use a preparation that requires as few applications as possible. The best candidate for drug therapy would therefore be treatment (A) metronidazole 25% applied 1 × a week for 2 weeks.     

Clinical evaluation of subgingival application of metronidazole 25%, and adjunctive therapy

The effect of topical application of a metronidazole gel (ELYZOL DENTAL GEL), and adjunctive therapy in the treatment of adult periodontitis was assessed clinically. A single, masked examiner performed clinical assessments. Fourteen patients were involved, each one received four different treatments including control, and the four treatments were randomly applied to at least one tooth in each quadrant for each patient in a comparative split-mouth design. Clinical examinations were carried out before treatment and 90 days after treatment. All patients had at least one tooth in each quadrant with probing pocket depth of > or = 5mm. The four treatment groups were: (I) One session of one hour of scaling and root planning, (II) metronidazole 25% dental gel (ELYZOL DENTAL GEL) applied on day 0 and day 7, (III) scaling adjunctive to metronidazole 25%, and (IV) No treatment. Instruction in oral hygiene was given to all subjects at base line examination. At the end of the study (day 90), all groups had statistically significant improvement in probing pocket depth (P < 0.02), and in plaque and bleeding indices (P < 0.05) when compared to day 0. However, group III had statistically significantly greater improvement (P < 0.03) in probing pocket depth than groups I, II and IV. Both groups I and II had statistically significantly greater improvement (P < 0.05) in probing pocket depth than control group. On the other hand, both groups were not statistically significantly different from each other in probing pocket depth improvement. It is suggested that topical Elyzol treatment may improve periodontal health as well as subgingival scaling and root planning therapy, and adjunctive treatment could obtain an additional therapeutic effect.     

Comparison of clinical outcomes following treatment of chronic adult periodontitis with subgingival scaling or subgingival scaling plus metronidazole gel

BACKGROUND, AIMS: Conventional treatment of chronic periodontitis involves mechanical debridement of periodontal pockets. Recently, subgingival antimicrobials have been used adjunctively following such debridement. This 2-centre study compared the clinical effects of subgingival scaling (SRP) with SRP plus subgingival application of 25% metronidazole gel, Elyzol (SRP+gel), in patients with chronic adult periodontitis. METHOD: Voluntary informed written consent was obtained from 45 subjects at the Eastman (mean age 46, range 34-63) and 43 subjects at RAF Halton (mean age 47, range 34-71) who participated in this blind, randomised split-mouth design study. All had at least 2 sites in each quadrant with probing pocket depth (PPD) > or = 5 mm. PPD, bleeding on probing (BOP), and clinical probing attachment levels (CAL) measured using a stent, were recorded at baseline and at 1, 3, 6 and 9 months post-therapy. After subgingival scaling of all quadrants, 2 quadrants were randomly selected to be treated with metronidazole gel. RESULTS: A paired t-test on baseline values showed no bias between groups. Both treatments effectively reduced the signs of periodontitis. At each follow-up visit, reduction in PPD, CAL and BOP after the combined treatment was greater than for SRP alone. Paired t-tests showed that the improvement in the SRP+gel group was statistically significantly better (p<0.001) than for SRP alone (mean 0.5 +/- 0.6 mm. 95% CI 0.4-0.6 mm.) Similarly, the % of sites which improved to a final pocket depth of < or = 3 mm and the % of sites which improved over the 9 months of the study by as much as > or = 2 mm were greater for SRP+gel than for SRP alone. CONCLUSIONS: At the end of the study, the mean reductions for PPD were 1.0 mm (SRP) compared to 1.5 mm (SRP+gel), and for CAL they were 0.4 mm (SRP) compared to 0.8 mm (SRP+gel), with mean difference for CAL between treatments of 0.4 +/- 0.6 mm (95% confidence intervals of 0.3-0.6 mm). The combination therapy of SRP+gel was superior to the conventional treatment of SRP alone, and these differences were maintained for 9 months.     

Repeated local metronidazole-therapy as adjunct to scaling and root planing in maintenance patients

The purpose of this investigation was to evaluate the effect of local antibiotic therapy with metronidazole adjunctively to scaling and root planing (SRP) versus mechanical treatment alone. 30 maintenance-patients were included in this single-blind study. The subjects had to comply with the following criteria: 2 non-adjacent sites with a probing depth > or =6 mm with bleeding on probing in separate quadrants, no periodontal therapy within the last 3 months, and no antibiotic therapy within the last 6 months. After randomization, the study sites were assigned to one of the following 2 treatments: SRP plus subgingival application of metronidazole 25% dental gel (Elyzol) 5x during 10 days (test site) or SRP alone (control site). Subgingival microbiological samples were taken prior to, and 21 days and 3 months after scaling. The samples were analyzed with a commercial chair-side ELISA (Evalusite) for Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans. Probing pocket depth (PPD), attachment level (AL) and bleeding on probing (BOP) were recorded at baseline and 3 months later. PPD reduction and AL-gain were statistically significant (p<0.001) after both treatments. However, there were no statistically significant differences between them. The same observation was made for BOP. P. gingivalis was reduced significantly after both treatments without statistically significant differences. P. intermedia was reduced significantly only after SRP. A. actinomycetemcomitans was not reduced significantly after either treatment. In conclusion, the repeated local application of metronidazole as an adjunct to SRP and the mechanical treatment alone showed similar clinical and microbiological effects without statistically significant differences with the exception of P. intermedia.