血清神经元特异性烯醇化酶在淋巴瘤诊断中的价值

目的：探讨神经元特异性烯醇化酶（NSE）与淋巴瘤诊断的关系,是否可作为淋巴瘤的肿瘤标志物。方法：采用电化学发光免疫测定法测定淋巴瘤及对照组患者的血清NSE值。有高热的临床表现,最后诊断排除恶性疾病的患者为对照组。结果：28例恶性淋巴瘤患者血清NSE值（29．37±7．94）ug／L明显高于对照组（11．45±2．85）ug／L,差异有统计学意义（P〈0．01）。结论：NSE对于淋巴瘤的诊断具有较高灵敏度;是否可作为淋巴瘤的标志物,尚需扩大研究例数。     

脑梗死患者血清神经元特异性烯醇化酶的变化

神经元特异性烯醇化酶(NSE)的研究为诊断神经系统疾病提供了新方法,为了探讨脑梗死患者血清神经元特异性烯醇化酶的变化,本文就急性脑梗死(ACI)患者血清神经元特异性烯醇化酶进行了检测,现报道如下。     

癫痫患者脑组织海马神经元的形态变化和神经元特异性烯醇化酶表达

目的 观察癫痫患者脑组织中海马齿状回颗粒细胞层神经元的形态学变化和神经元特异性烯醇化酶(NSE)在脑组织中的表达,探讨癫痫患者脑组织海马神经元的病理学改变与癫痫发病的关系.方法 脑海马组织标本来自于癫痫手术切除病人(9例)和正常无精神与神经疾病的尸检者(20例).HE染色,光镜下观察脑组织中海马区齿状回颗粒细胞层神经元形态学改变,免疫组织化学方法 检测NSE表达,比较两组NSE阳性表达率.结果 癫痫患者脑组织海马颗粒细胞层中均可见核固缩的神经元,约占全部神经元的15.80%,还可见到肿胀的神经元,NSE阳性表达的神经元呈棕黄色,在出现核固缩的神经元中,发生NSE阳性表达占28.66%,癫痫病人脑组织海马NSE阳性表达率为(7.9±5.6)%.对照组脑组织海马神经无形态正常,细胞核大而圆,位于胞体中央,核仁明显,NSE阳性表达率为(39.0±17.4)%.与对照组相比,癫痫患者含棕黄色颗粒的神经元数目减少,两组NSE阳性表达率比较,差异有统计学意义(t＝-5.13,P＜0.01).结论 癫痫患者脑组织中海马神经元形态改变和NSE表达异常,可能是癫痫患者发病的主要因素之一.Study on the pathological change and the expression of neuron specific enolase in the hippocampus dentategyrus granular cell layer of epilepsy patients     

神经元特异性烯醇化酶异常表达对肺癌诊断价值的研究

肺癌一直是危害人类健康的多发病和常见病,也是当今恶性疾病死亡的主要原因之一。神经元特异性烯醇化酶(ＮＳＥ)是糖酵解中的酶烯醇化酶的同工酶,广泛存在于中枢和周围神经组织及小细胞肺癌(ＳＣＬＣ)中,近来发现在非小细胞肺癌(ＮＳＣＬＣ)中也有升高。对各种肺癌的组织、血清进行了ＮＳＥ测定,以探讨ＮＳＥ在肺癌中的作用。材料与方法　(1)人肺组织标本:收集1999年4月～1999年8月手术后新鲜肺癌组织21份。立即于-85℃冰箱保存。经病理切片诊断,鳞癌12例,腺癌7例,小细胞肺癌2例。(2)肺癌病人血清:收集本院1998年～1999年住院病人中的原发性…     

巴曲酶对急性脑梗死患者血清神经元特异性烯醇化酶和神经功能的影响

目的观察巴曲酶对急性脑梗死患者血液中神经元特异性烯醇化酶（NSE）的影响,探讨其对脑神经功能的保护作用。方法 102急性脑梗死患者随机分为治疗组52例、对照组50例。对照组给予常规治疗,治疗组在此基础上加用巴曲酶治疗。观察治疗前后两组血清NSE浓度以及神经功能缺损评分变化。结果巴曲酶治疗组第7天血液中NSE浓度明显下降,低于对照组（P〈0.05）,第14、28天神经功能评分也明显少于对照组（P均〈0.05）。结论巴曲酶可减轻脑梗死患者神经元损伤,能有效改善急性脑梗死患者的神经功能缺损。     

神经元特异性烯醇化酶对心肺复苏后患者脑损伤的诊断价值

随着心肺复苏(CPR)技术的不断改进,25%~50%的心跳骤停患者能够恢复自主循环,但出院率只有2%~14%的低水平,主要原因是顽固性的脑损伤[1]。目前临床上缺少可靠的评价脑损伤早期诊断手段,而心肺复苏后对脑损伤程度及预后的评价又影响CPR后的治疗方案,因此探讨早期诊断脑损伤指标意     

脑卒中患者血清神经元特异性烯醇化酶变化及意义

用免疫学方法测定脑梗死和脑出血患者的血清神经元特异性烯醇化酶(N SE)水平,并与正常健康者进行对照。结果显示,脑梗死患者血清N SE(30.54±12.66)ng/m l,脑出血患者(31.30±13.78)ng/m l,均显著高于对照组的(13.89±6.76)ng/m l(P<0.05);脑梗死和脑出血患者发病后不同时期血清N SE有明显变化,N SE水平与脑损伤程度呈正相关,与病情转归及患者预后有明显关系。提示血清N SE水平是急性脑血管病所致脑损害较敏感的生化指标,可较早反映脑损伤程度,并与病情的严重程度及预后关系密切,有助于其早期诊断、指导临床和评价疗效。     

甘露醇对急性脑梗死血清神经元特异性烯醇化酶的影响

目的观察20%甘露醇注射液对急性脑梗死患者血液中神经元特异性烯醇化酶的影响,探讨甘露醇对脑的保护作用。方法利用酶联免疫吸附法检测35例脑梗死患者甘露醇注射液治疗过程中血清中的神经元特异性烯醇化酶(neuron-specific enolase NSE)浓度,记录神经功能NIHSS评分,评定临床疗效,并与常规治疗组进行比较。结果甘露醇治疗组第3、7天血液中NSE浓度明显下降,低于常规治疗组,神经功能评分也明显少于常规治疗组(P<0.05)。结论甘露醇对脑神经元有保护作用,能有效改善急性脑梗死患者的神经功能缺损。     

脑梗死急性期血清中神经元特异性烯醇化酶水平的变化及其临床意义

目的探讨脑梗死患者急性期血清中神经元特异性烯醇化酶(NSE)水平的变化及临床意义。方法采用ELISA法测定了56例脑梗死患者急性期和30例性别、年龄结构相匹配的健康人血清中NSE水平,应用CT扫描测定脑梗死体积和斯堪的那维亚卒中量表(SNSS)进行神经功能缺损评分。结果脑梗死患者急性期血清中NSE水平显著高于对照组(P<0.01),血清中NSE水平与脑梗死面积和神经功能缺失程度呈显著正相关(P<0.05)。结论脑梗死患者急性期血清中NSE水平增高与神经元坏死有关,NSE水平的高低可以反映脑梗死面积的大小,为脑梗死后神经元损伤程度提供定量信息。     

急性脑梗死血清中神经元特异性烯醇化酶水平的变化及意义研究

目的探讨急性脑梗死患者血清中神经元特异性烯醇化酶（NSE）水平的变化及意义。方法采用ELISA法测定40例急性脑梗死患者和30例性别、年龄结构相匹配的健康人血清中NSE水平,应用CT扫描测定脑梗死体积和斯堪的那维亚卒中量表（SNSS）进行神经功能缺损评分。结果急性脑梗死患者血清中NSE水平显著高于对照组（P〈0.01）,血清中NSE水平与脑梗死面积和神经功能缺失程度呈显著正相关（P〈0.05）。结论急性脑梗死患者血清中NSE水平增高与神经元坏死有关,NSE水平的高低可以反映脑梗死面积的大小,为脑梗死后神经元损伤程度提供定量信息。     

Enzymatic determination of serum neuron-specific enolase in small cell lung cancers. Utility of the serum neuron-specific enolase/serum nonneuronal enolase ratio

Increasing interest is shown in the determination of the serum neuron-specific enolase for the diagnosis and the follow-up studies of small cell lung cancers. We report results obtained by an enzymatic procedure that permits the simultaneous determination of the neuron and nonneuron-specific enolase and the calculation of the ratio of these two components. The utility of this ratio which characterizes elevations of the serum neuron-specific enolase from a poor or rich source of this component was tested in 38 patients with small cell lung carcinoma and in 57 subjects suffering from other bronchogenic cancers. The control group consisted of 37 blood donors and 56 patients with respiratory disease. For the diagnosis, the sensitivity and the specificity of the enzymatically determined neuron-specific enolase compared well with published results obtained by radioimmunoassay and enzymoimmunoassay. The use of the ratio clearly increases the specificity of the test, since only 5.3 percent of false positive results are found when bronchogenic tumors other than small cell carcinoma are studied. The sensitivity was 76 and 100 percent in diagnosis of limited and extensive forms, respectively. The use of this ratio in the follow-up of the patients and for the determinations in hemolyzed samples is set out.     

Pyothorax associated lymphoma with increased neuron-specific enolase level in serum and pleural effusion: a case report

A 72-year-old male with a past history of artificial pneumothorax for pulmonary tuberculosis at the age of 25 was referred to our hospital for the clinical signs of pain and swelling of the back. His neuron-specific enolase values were high in both serum and pleural effusion. The computed tomography image showed a tumor mass arising from the wall of chronic pyothorax. The tumor was resected including the wall of the chronic pyothrax and right chest wall with several ribs. The tumor was 7.2 x 7.0 x 3.0 cm in size and the pathological diagnosis was non-Hodgkin's lymphoma diffuse large cell, B-cell type. Postoperative chemotherapy and radiation therapy were performed but he died of recurrence and metastasis of the tumor 5 months later after the operation.     

Clinical significance of serum neuron-specific enolase in patients with adult T-cell leukemia

The present study examines the clinical significance of serum neuron-specific enolase (NSE) in patients with adult T cell-leukemia (ATL). Serum NSE values were measured using a radioimmunoassay in 35 patients (acute type, n = 15; lymphoma type, n = 10; chronic type, n = 10) and in 7 controls carrying T lymphotropic virus type-1 (HTLV-1). Serum NSE values >10 ng/mL were detected in 9 of 15 patients with acute type (60%), 5 of 10 with lymphoma type (50%), and in one of 10 patients with chronic type (10%) ATL, but in none of the HTLV-1 carriers. Contrary to previous findings demonstrating that 20% of patients with non-Hodgkin's lymphoma (NHL) had positive serum NSE, the frequency of a high NSE value in patients with acute and lymphoma type ATL was much higher (60% and 50%, respectively). The serum NSE value positively correlated with serum thymidine kinase activity (TK) and serum soluble interleukin-2 receptor (sIL-2R) levels (P < 0.04 and P < 0.01, respectively). Serum NSE values at the initial diagnosis were adversely related to overall survival time according to the log-rank test (P < 0.02). Pathological examinations demonstrated that both patients with anaplastic large cell lymphoma type ATL had cytoplasmic NSE and CD30 markers on cell membranes. These findings suggest that serum NSE is partially produced by ATL cells and that ATL tumor cells seem preferentially produce NSE compared with other NHL cells. Serum NSE may be a novel marker of disease aggressiveness as well as a prognostic factor for ATL.     

Immunohistochemical study of neuron-specific enolase and CA 19-9 in pancreatic disorders. The value of neuron-specific enolase as a marker for islet cell and nerve tissue

Immunohistochemical studies of neuron-specific enolase were performed on pancreatic tissues from patients with insulinoma, nonfunctioning islet cell tumor, chronic pancreatitis, and pancreatic adenocarcinoma, and from 5 normal patients. The concentration of neuron-specific enolase was also measured in the sera of patients and in the pancreatic tissue, and the tissues were stained for carbohydrate antigen 19-9 by immunohistochemical techniques. Neuron-specific enolase was localized in nerve fibers, normal islet cells, and islet cell tumors; its concentration was elevated only in the tissue of islet cell tumors and in serum from patients with insulinoma. In the pancreatic tissue of pancreatitis or pancreatic adenocarcinoma, various changes in acini and islets were present. The altered islets stained clearly for neuron-specific enolase and could easily be distinguished from altered, unstained acini in cases of pancreatitis or pancreatic adenocarcinoma. Islets in the pancreatic tissue remained intact with various morphologic changes, although acini had degenerated severely. Carbohydrate antigen 19-9 was localized in all the carcinoma cells in the pancreatic tissue and in some of the normal pancreatic ducts. No cells were simultaneously immunostained by anti-neuron-specific enolase and anti-carbohydrate antigen 19-9 antibodies. Thus, neuron-specific enolase is a good marker for islet cell tumor, and is valuable for examining islets in pancreas with various disorders both alone and in combination with other tumor markers.     

Prinzmetal angina in the differential diagnosis of syncope

Prinzmetal (variant) angina may be associated with cardiac arrhythmias that can deteriorate to fatal ventricular arrhythmias. We present 2 patients with syncope where vasospastic angina and severe ventricular arrhythmias were found to be responsible for the syncopal episodes.     

Correlation analysis between serum neuron-specific enolase and the detection of gene mutations in lung adenocarcinoma

BackgroundLung cancer is a chronic, progressive and malignant disease associated with ever-growing incidence and mortality. Targeted therapy plays an important role in the clinical treatment of lung cancer. Besides, neuron-specific enolase (NSE), an intracellular enzyme, is highly correlated with the targeted treatment outcome in patients with non-small cell lung cancer (NSCLC). The present study aimed to explore the correlation of NSE with the detection of gene mutations.MethodsIt is a case-control study. From June 2017 to October 2019, the newly diagnosed patients with lung adenocarcinoma were enrolled from the First Affiliated Hospital of Anhui Medical University. Next-generation sequencing (NGS) was conducted in these patients. Kruskal-Wallis test was used to calculate the difference in NSE levels between mutant and non-mutant group and the differences were compared between blood and tissue samples.ResultsCompared with patients with no gene mutation (15.4±7.8 mmol/L), the NSE levels in patients with gene mutations were remarkably increased in blood sample group (22.2±12.9 mmol/L) (P<0.05). Besides, the linear regression model was applied for analysis which further emphasized the close relationship between them. The area under the ROC curve (AUC) of NSE was 0.7300 [95% confidence interval (CI): 0.6059–0.8541] and optimal threshold was 18.5650 U/mL with a sensitivity of 87.50% and a specificity of 52.08%. In addition, NSE levels increased in blood sample group, suggesting that the occurrence of polygenic mutation with dismal prognosis, but no correlation was detected in tissue sample group.ConclusionsThis study elucidates the functional role of NSE, and findings in this study notably increase the gene detection efficiency for lung adenocarcinoma.