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1.
本文用放射免疫测定法及双抗体夹心法测定了25例健康人,30例肝硬化患者血清透明质酸(HA)的含量及可溶性白细胞介素2受体(sIL-2R)水平。结果表明:(1)肝硬化组血清HA含量及sIL-2R水平均高于对照组(P值均<0.01);(2)肝硬化组血清HA与sIL-2R水平间呈正相关关系(r=0.5192,P<0.01)。提示:肝硬化组患者血清sIL-2R水平增高与肝损害程度有关,可能是由于肝细胞受损而对SIL-2R清除能力降低所致。  相似文献   

2.
本文采用细胞ELISA法,研究发现人巨细胞病毒(HCMV)感染对单核细胞HLA-DR的影响。结果表明HCMV感染后1d,单核细胞HLA-DR表达显著增高(P<0.01),以后逐渐降低,d5降至对照水平;IFNγ(500U/ml).TNF(250U/ml)、IL-6(500/ml)、IL-1(500/ml)均能不同程度地刺激单核细胞HLA-DR表达;HCMV感染后,细胞因子刺激HLA-DR表达的水平在感染后d5,较对照组均显著降低(P<0.01);IL-1+IFN-γ及TNF+IFN-γ在刺激单核细胞HLA-DR表达时有协同作用;HCMV感染后,IFN-γ+IL-1及TNF+IFN协同刺激单核细胞HLA-DR表达水平较对照组显著降低(P<0.01)。结果提示:在HCMV感染引起免疫抑制过程中,其引起单核细胞HLA-DR表达降低是一重要机制。  相似文献   

3.
HCMV感染对单核细胞HLA-DR表达的影响   总被引:1,自引:0,他引:1  
本文采用细胞ELISA法,研究发现人巨细胞病毒(HCMV)感染对单核细胞HLA-DR的影响。结果表明HCMV感染后1d,单核细胞HLA-DR表达显著增高(P<0.01),以后逐渐降低,d5降至对照水平;IFNγ(500U/ml).TNF(250U/ml)、IL-6(500/ml)、IL-1(500/ml)均能不同程度地刺激单核细胞HLA-DR表达;HCMV感染后,细胞因子刺激HLA-DR表达的水平在感染后d5,较对照组均显著降低(P<0.01);IL-1+IFN-γ及TNF+IFN-γ在刺激单核细胞HLA-DR表达时有协同作用;HCMV感染后,IFN-γ+IL-1及TNF+IFN协同刺激单核细胞HLA-DR表达水平较对照组显著降低(P<0.01)。结果提示:在HCMV感染引起免疫抑制过程中,其引起单核细胞HLA-DR表达降低是一重要机制。  相似文献   

4.
食管癌患者五项细胞免疫学指标的测定及其意义   总被引:3,自引:0,他引:3  
杨正  王艾丽 《现代免疫学》1996,16(6):361-362
对35例食管癌(EC)患者和30例正常人(NC)的外周血自然杀伤(NK)细胞活性、T细胞亚群、血浆可溶性白细胞介素2受体(sIL-2R)、肿瘤坏死因子α(TNF-α)以及外周血诱生γ干扰素(IFN-γ)的水平进行了检测。结果显示,EC患者NK细胞活性明显降低(P<0.01);CD8+细胞增多(P<0.06),CD4+/CD8+细胞比值降低(P<0.05);sIL-2R明显增高(P<0.01);外周血诱生IFN-γ降低(P<0.05);血浆TNF-α升高。表明,上述指标对判断、分析EC患者细胞免疫功能状况及病变程度有较大的意义。  相似文献   

5.
细胞因子水平与晚期胃癌患者治疗及预后相关研究   总被引:21,自引:0,他引:21  
本文报道了249例晚期胃癌(Ⅲ~Ⅳ期)患者外周血单个核细胞(PBMC)产生IL-2水平及IL-2R表达,外周血清中sIL-2R浓度与PBMC被PHA-P诱生后产生IFN-γ活性;并探讨了姑息性手术、化疗和中药治疗对晚期胃癌患者细胞免疫调节因子水平的影响。结果发现:晚期胃癌患者外周血PBMC产生IL-2及IFN-γ能力及IL-2R表达均显著低于早期胃癌患者和正常对照组水平(P<0.01);sIL-2R水平前者明显低于后二者(P<0.01)。姑息手术治疗尽管对晚期胃癌患者的IL-2、IL-2R和IFN-γ水平无显著影响,但可降低其sIL-2R水平;而扶正抗癌冲剂则可是显著提高患者术后IL-2及IFN-γ水平和IL-2R表达,同时降低sIL-2R水平(P<0.01);化疗有效晚期患者,则可导致其sIL-2R水平下降,无效者则sIL-2R水平无改变或继续升高。三年随访发现,晚期胃癌患者,术前IL-2产生水平高于100U/ml,同时sIL-2R水平低于1000U/ml者,其三年生存率高。因而,这项研究对晚期胃癌治疗疗效判断及预后评价有指导意义。  相似文献   

6.
本文用放射免疫测定法及双抗体夹心法测定了25例健康人,30例肝硬化患者血清透明质酸(HA)的含量及可溶性白细胞介素2受体(sIL-2R)水平。结果表明:(1)肝硬化组血清HA含量及sIL-2R水平间呈正相关关系(r=0.5192,P〈0.01)。提示:肝硬化组患者血清sIL-2R水平增高与肝损害程度有关,可能是由于肝细胞受损而对sIL-2R清除能力降低所致。  相似文献   

7.
用PCR检测HCMV-DNA,ELISA法检测HCMV-IgM及IgG,以诊断肾移植受者HCMV感染。用双抗体夹心法ELISA检测65例肾移植受者血清sIL-2R水平,结果表明:HCMV感染后宿主血清sIL-2R水平明显增高(P<0.01),且HCMV疾病组sIL-2R增高程度大于无症状感染组(P<0.01);6例原发性HCMV感染者sIL-2R水平与IgM水平呈正相关(r=0.9908),提示随感染程度增加,血清sIL-2R水平随之增高,还发现血清sIL-2R水平与C94/CD8比值是负相关(r=-0.9789),说明HCMv感染后sIL-2R水平增高与T细胞亚群改变有关,反之也说明sIL-2R增高程度可表明体内免疫抑制状态。对于HCMV感染后血清sIL-2R水平增高的机理有待进一步探讨。  相似文献   

8.
检测16例HDV(+)重肝患者PBMC膜上IL-2R、培养上清和血清中sIL-2R含量,以21例HDV(-)重肝为疾病对照,20例献血员为正常对照,探讨IL-2R在丁型肝炎中变化意义。结果:HDV(+)组IL-2R表达虽增强,但不如HDv(-)组明显;sIL-2R含量,两重肝组均非常显著增高,但之间无差异。提示HDV(+)重肝组T淋巴细胞被激活状态并未因增加HDV感染而加重,支持丁型肝炎肝损伤主要不是免疫介导的观点。  相似文献   

9.
本文采用双抗体夹心ELISA法检测了38例登革热(DF)患者血清中可溶性白细胞介素2受体(sIL-2R)的水平。结果显示DF患者血清sIL-2R明显升高,sIL-2R的水平与患者发热时间长短及皮疹性质(充血性和出血性皮疹)有关,且与患者尿蛋白含量呈正相关。表明sIL-2R时判断DF的病情及肾脏损害程度有一定的临床意义,并提示sIL-2R可能参与了DF的免疫病理过程。  相似文献   

10.
陈志伟  李贵华 《现代免疫学》1996,16(4):239-239,226
本文采用双抗体夹心ELISA法检测了38例登革热(DF)患者血清中可溶性白细胞介素2受体(sIL-2R)的水平。结果显示DF患者血清sIL-2R明显升高,sIL-2R的水平与患者发热时间长短及皮疹性质(充血性和出血性皮疹)有关,且与患者尿蛋白含量呈正相关。表明sIL-2R时判断DF的病情及肾脏损害程度有一定的临床意义,并提示sIL-2R可能参与了DF的免疫病理过程。  相似文献   

11.
Renal dysplasia and asplenia in two sibs   总被引:2,自引:0,他引:2  
A family is reported in which two sibs, one male and the other female, both died within 24 hours of birth with enlarged polycystic kidneys. Postmortem histology in the second child showed gross renal dysplasia. In both children the pancreas was enlarged, nodular and cystic but the liver appeared macroscopically normal. In the second child, histological examination confirmed pancreatic fibrosis with cystic dilation of ducts, but showed portal fibrosis with bile duct proliferation in the liver.
This combination of findings is very reminiscent of those in a girl and her brother reported by Ivemark et al. (1959). The children reported here also showed absence or hypoplasia of the spleen, cardiac anomalies and other features of the Ivemark syndrome (Ivemark 1955), a quite different, usually sporadic, congenital disorder. It is suggested that the children described here have a distinct lethal congenital disorder, probably inherited in an autosomal recessive manner.  相似文献   

12.
Over 200 schizophrenic patients belonging to three major and interrelated pedigree complexes have been investigated over the past 30 years in a North Swedish geographically isolated population, presently numbering about 6,000. An intensive investigation of a number of biochemical correlates and genetic markers in a few selected families belonging to one of the major pedigrees has indicated new strategies for the current research program.
Schizophrenia, as defined operationally, is significantly associated with decreased activities of two enzymes (1) blood platelet monoamine oxidase, (2) plasma dopamine-β-hydroxylase, and (3) with the genetic marker Gc2 (group specific antigen). Both enzymes are subject to genetic variation. A positive score for linkage between schizophrenia and low plasma DBH activity has been calculated, but, so far, available data are insufficient for discrimination between linkage and partial contribution of genetically controlled low plasma DBH to the pathogenesis of the disease. Alternatively, both mechanisms could be involved.
As a model for continued research, schizophrenia is explained as based on a double dominant-recessive genotype (Aabb), representing a vulnerability which in about 50 % of cases develops into clinical schizophrenia. It is suggested that the dominant mutation (A) operates on or affects MAO activity, and that the recessive genotype (bb) is instrumental in low variates of DBH activity and very likely such variates within the normal range of physiological variation. Moreover, it is suggested that the combined effects of MAO- and DBH-reduced efficiency on the metabolism of e.g. dopamine could be an essential pathogenic mechanism for the schizophrenic illness which is segregating in this population.  相似文献   

13.
There are an estimated over 200 million yearly cases of malaria worldwide. Despite concerted international effort to combat the disease, it still causes approximately half a million deaths every year, the majority of which are young children with Plasmodium falciparum infection in sub-Saharan Africa. Successes are largely attributed to malaria prevention strategies, such as insecticide-treated mosquito nets and indoor spraying, as well as improved access to existing treatments. One important hurdle to new approaches for the treatment and prevention of malaria is our limited understanding of the biology of Plasmodium infection and its complex interaction with the immune system of its human host. Therefore, the elimination of malaria in Africa not only relies on existing tools to reduce malaria burden, but also requires fundamental research to develop innovative approaches. Here, we summarize our discoveries from investigations of ethnic groups of West Africa who have different susceptibility to malaria.  相似文献   

14.
About 1900, modern food selection and processing caused widespread epidemics of the B vitamin deficiency diseases of beriberi and pellagra which, for genetic reasons, often expressed as different diseases ranging from bowel and heart disease to dermatoses and psychoses. But the B vitamins merely help convert essential fatty acids (EFA) into the prostaglandin (PG) tissue regulators and it now turns out that, through hydrogenation, milling and selection of w3-poor southern foods, we have also been systematically depleting, by as much as 90%, a newly discovered trace Nordic EFA (w3) of special importance to primates and sole precursor of the PG3(4) series, even as a concurrent fiber deficiency increases body demand for EFA. Since substrate EFA is processed by many B vitamin catalysts, an EFA deficiency will mimic a panhypovitaminosis B, i.e., a mixture of substrate beriberi and substrate pellagra resembling vitamin beriberi and pellagra but exhibiting as even more diverse endemic disease. This would consitute a second stage of the Modern Malnutrition and explain why some workers now hold the dominant diseases of modermized societies to be new, nutritionally based, pellagraform yet lipid-related and to range, once again, from heart disease to psychosis. It is an assumption that our dominant diseases are unrelated to each other or are merely revealed by our diagnostic acumen and therapeutic success; and that hydrogenating millions of tons of food oils annually, to destroy the rancidity producing w3-EFA, is safe for primates. Extensive beriberiform disease is reported here in 32 typical cases taken from medical practice which responds strikingly to linseed oil supplements (60% w3-EFA) in confirmation of identical results in Capuchins.  相似文献   

15.
Most bodily functions require the coordinated actions of complementary and supplementary paired muscle groups. Where this essential muscular cooperation is lacking, hollow organs may burst and others become literally screwed up, giving rise to many similar spastic diseases such as Torticollis, Twisted ovarian cyst, Torsion of the Testis, Volvulus of the intestines, Varicose Veins, Megacolon, Aortamegaly, Scoliosis, Erb's Palsy, Peyronie's Disease, Main-en-Griffe, Undescended Foot (Pes Cavus), Talipes, Strabismus. Spasm is “panenepidemic” and unclassified examples of Torsion Dystonia and Dyskinesia really are as common as debt and taxes.  相似文献   

16.
17.
《Human immunology》2022,83(11):739-740
Georgia (or Sakartvelo in its own language) is a South Caucasus Mts. country with its easternmost part is enigmatically named Iberia, like the Iberian Peninsula, which may refer to rivers “Kura” and “Ebro” or their valleys respectively. Most of their inhabitants speak Georgian which is included within Dene-Caucasian group and Usko-Mediterranean subgroup of languages. The latter includes Basque, Berber, ancient Iberian-Tartessian, Etruscan, Hittite, Minoan Lineal A and others. In the present paper, HLA class II -DRB1 and -DQB1 alleles has been studied and extended haplotypes calculated. Most frequent haplotypes are also of Mediterranean origin (i. e.: (A*02-B*51)-DRB1*11:01-DQB1*03:01, (A*02-B*51)-DRB1*13:01-DQB1*06:03, or (A*24-B*35)-DRB1*01:01-DQB1*05:01) and DA genetic distances show that closest world populations to Georgians are Mediterraneans. Georgians also show common extended haplotypes ((A*02-B*51)-DRB1*11:01-DQB1*03:01, (A*02-B*13)-DRB1*07:01-DQB1*02:01 and (A*03-B*35)-DRB1*11:01-DQB1*03:01) with Svan people, a secluded population in North Georgia mountains. We can conclude that Georgians belong to a very old Mediterranean substratum according to both linguistics (Usko Mediterranean languages) and HLA genetics.  相似文献   

18.
Newton H 《Medical history》2011,55(2):153-182
Sick children were ubiquitous in early modern England, and yet they have received very little attention from historians. Taking the elusive perspective of the child, this article explores the physical, emotional, and spiritual experience of illness in England between approximately 1580 and 1720. What was it like being ill and suffering pain? How did the young respond emotionally to the anticipation of death? It is argued that children’s experiences were characterised by profound ambivalence: illness could be terrifying and distressing, but also a source of emotional and spiritual fulfilment and joy. This interpretation challenges the common assumption amongst medical historians that the experiences of early modern patients were utterly miserable. It also sheds light on children’s emotional feelings for their parents, a subject often overlooked in the historiography of childhood. The primary sources used in this article include diaries, autobiographies, letters, the biographies of pious children, printed possession cases, doctors’ casebooks, and theological treatises concerning the afterlife.  相似文献   

19.
Recent advancements in agricultural biotechnology have created a need for analytical techniques to determine introduced proteins in crops enhanced through modern biotechnology techniques. These proteins are expressed in plant tissues and may be present in food ingredients. Immunoassays are ideally suited for protein detection and may be used as both quantitative and threshold methods. Microplate ELISA and lateral flow devices are two of the most commonly used immunoassay formats for agricultural biotechnology applications. This paper provides general background information and a discussion of criteria for the validation and application of immunochemical methods to the analysis of proteins introduced into plants and food ingredients using biotechnology methods. It is the result of a collaborative effort of members of the Analytical Environmental Immunochemical Consortium. This collaborative effort represents the combined expertise of several organizations to reach consensus on establishing guidelines for the validation and use of immunoassays. Further, the paper offers developers and users a consistent approach to adopting the technology as well as aid in producing accurate and meaningful results.  相似文献   

20.
The preparation steps usually necessary for obtaining ultrathin frozen sections of biological material (chemical prefixation, enclosing, cryoprotective treatment, freezing, sectioning, and post-staining the sections for transmission electron microscopy) are submitted to a critical analysis. The application of cryo-ultramicrotomy, in particularly for cytochemical purposes, is reviewed. Fundamental considerations of chemical prefixation and poststaining are supported by examples from yeast cytology. Furthermore, the efficiency of the cryo-ultramicrotomy (electron optical resolution of ultrastructural details) is demonstrated on yeast cells and protoplasts.  相似文献   

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