血液灌流对重症急性胰腺炎大鼠血液中细胞因子的影响

观察血液灌流对重症急性胰腺炎(SAP)大鼠血液中细胞因子的影响.方法:采用经胰管注射牛黄胆酸钠制造大鼠SAP模型,用NK-107树脂对SAP大鼠行血液灌流并测定血液中白细胞介素1(IL-l)、白细胞介素6(IL-6)及肿瘤坏死因子(TNF)的变化情况.结果:SAP大鼠血液中的IL-1、IL-6、TNF在发病早期可明显升高,通过血液灌流可使这些细胞因子的水平下降.从而使SAP大鼠的病死率由53.33%降至25.93%,存活时间平均由24.20±5.60小时延长至30.50±630小时.结论:NK-107树脂血液灌流对SAP有一定治疗作用.     

急性胰腺炎患者血清TNF-α、IL-6和IL-8含量测定

研究血清TNF α、IL 6和IL 8在急性胰腺炎 (AP )发生发展中的作用和临床意义。采用双抗体夹心ELISA法测定 41例AP患者及正常对照组 40例血清TNF α、IL 6、IL 8和C 反应蛋白 (CRP )水平。结果显示 ,重症急性胰腺炎 (SAP )和轻症急性胰腺炎 (MAP )血清TNF α、IL 6、IL 8和CRP水平较正常对照组明显增高 (P <0 0 5 ) ,SAP组又较MAP组增高 (P <0 0 5 )。2 7例MAP患者血清TNF α、IL 6、IL 8和CRP水平在治疗后 3d已有下降 ,7d降至正常水平。 14例SAP患者血清TNF α、IL 6、IL 8和CRP水平在治疗后 7d明显降低 ,2 1d仍未降至正常水平。血清中TNF α、IL 6和IL 8可能与AP的严重程度相关 ,在临床上联合检测血清TNF α、IL 6、IL 8和CRP可用于AP的病情严重程度判定和观察治疗后疗效及病情恢复的情况     

N-乙酰半胱氨酸对重症急性胰腺炎大鼠肺损伤作用的实验研究

目的:探讨N-乙酰半胱氨酸(NAC)对重症急性胰腺炎(SAP)大鼠肺损伤的作用。方法:雄性SD大鼠30只,随机分为假手术组(SO组)、SAP组、NAC组。胆胰管逆行注射5%牛磺胆酸钠制备SAP模型,造模后30min腹腔注射5%NAC(0.2ml/100g)干预SAP模型,12h后处死大鼠。检测各组血清淀粉酶(AMY)、肺组织髓过氧化物酶(MPO)、胰腺和肺组织病理学评分、肺组织肿瘤坏死因子-α(TNF-α)和细胞间粘附分子-1(ICAM-1)mRNA表达的变化。结果:与SO组比较,SAP组AMY、MPO、胰腺和肺组织病理学评分明显升高(P<0.01),TNF-α和ICAM-1mRNA表达明显增强(P<0.01);应用NAC处理后,AMY和MPO水平下降,胰腺和肺组织损伤缓解,TNF-α和ICAM-1mRNA表达减弱,与SAP组有明显差异(P<0.01)。结论:NAC对SAP大鼠肺损伤具有保护作用,其机制可能与抑制肺组织TNF-α和ICAM-1mRNA的表达有关。     

丹参治疗重症急性胰腺炎的血液流变学及其相关指标的实验研究

目的 :观察丹参对犬重症急性胰腺炎 (SAP)的血液流变学及其相关指标的影响。方法 :实验分三组 :正常对照组 (NC)、重症急性胰腺炎组 (SAP)和复方丹参干预组 (TS)。用 5 %牛磺胆酸钠和牛胰蛋白酶混合液胰管注入 ,制备犬SAP模型。TS组于模型制备后 15min和 12h分别静脉滴注复方丹参(2ml/kg)。术后动态观察各组以下指标 :血液流变学、血小板活化因子(PAF)、血栓素A2 (TXA2 )、前列腺素I2 (PGI2 )、内皮素 (ET)和一氧化氮(NO)。结果 :SAP组TXA2 、PGI2 、ET、NO、PAF较NC组显著升高 (P <0 .0 1) ,血液流变学指标也有显著的改变 (P <0 .0 1) ;复方丹参对各项指标均有不同的影响 (P <0 .0 5 )。结论 :血液流变性、炎性介质PAF、二对血管活性物质TXA2 /PGI2 和ET/NO均是介导SAP胰腺血循环障碍的重要因素 ,复方丹参是有效的治疗措施之一。     

兔胸部爆炸伤后早期IL-6、IL-8和TNFα水平的变化

目的　研究实验性胸部爆炸伤后体内IL 6、IL 8和TNFα三种炎性细胞因子的变化。方法利用标准爆炸当量的 1 0g铝制雷管 ,电触发引爆造成家兔胸部爆炸伤模型 ,酶标免疫吸附法 (ELISA)测定血浆及支气管肺泡灌洗液中IL 6、IL 8和TNFα活性变化。结果胸部爆炸伤后肺泡灌洗液中上述三种细胞因子水平显著升高 ;外周血中上述因子水平亦升高 ,但程度较肺泡灌洗液中轻。不同程度爆炸伤组间肺泡灌洗液中三种细胞因子水平有统计学差异。结论IL 6、IL 8和TNFα三种炎性细胞因子与肺损伤关系密切 ,其水平变化在肺泡灌洗液中最为显著     

兔胸部爆炸伤后早期IL—1、IL—8和TNFα水平的变化

目的 研究实验性胸部爆炸伤后体内IL－1、IL－8和TNFα三种炎性细胞因子的变化。方法 利用标准爆炸当量的1．0g铝制雷管，电触发引爆造成家兔胸部爆炸伤模型，酶标免疫吸附法（ELISA）测定血浆及支气管肺泡灌认中IL－1、IL－8和TNFα活性变化。结果 胸部爆炸伤后肺泡灌洗液中上述三种细胞因子水平显著升高；外周血中上述因子水平亦升高，但程度较肺泡灌洗液中轻。不同程度爆炸伤组间肺泡灌洗液中三种细胞因子水平有统计学差异。结论 IL－1、IL－8和TNFα三种炎性细胞因子与肺损伤关系密切，其水平变化在肺泡灌洗液中最为显著。     

1α-羟化酶在重症急性胰腺炎大鼠肾脏中的表达及意义

<正>目的:观察1α-羟化酶在重症急性胰腺炎(SAP)大鼠肾脏组织中的表达规律及其意义。方法:48只Wistar大鼠随机分为两组:假手术组(SO组)和SAP组,每组再继续分为四个亚组,每组6只(n=6)。采用5%牛磺胆酸钠(STC)逆行胰胆管注射法建立SAP模型。分别于造模后1、3、6及12h分别心脏采血,测定血清淀粉酶、肌酐及尿素氮水平;取胰腺及肾脏组织固定,HE染色后,光镜观察胰腺和肾脏组织病理变化,并以Western Blotting法对肾脏组织中1α-羟化酶的表达水平和趋势进行分析。     

NK-107树脂吸附安眠药血液净化装置

本文报道了NK—107树脂吸附安眠药血液净化装置的性能、体外实验、动物实验及临床应用。用NK—107树脂吸附安眠药血液净化装置经血液灌流治疗重症药物中毒210例,治愈189例,治愈率为90％。本装置安全无毒,使用方便,适于临床抢救,便于推广应用。     

重症急性胰腺炎肺损伤发病机制中的肺泡Ⅱ型上皮细胞凋亡的作用及乌司他丁干预的实验研究

目的 探讨重症急性胰腺炎(SAP)发病机制中其肺泡Ⅱ型上皮细胞凋亡的作用并对乌司他丁对其干预的作用进行分析。方法 将42只健康大鼠随机分为3组,分别为假手术组、模型组与乌司他丁干预组,每组14只,以向大鼠十二指肠乳头内侧胰胆管注射脱氧胆酸钠建立SAP大鼠模型,假手术组大鼠仅打开大鼠腹腔轻轻翻动其胰腺后关闭腹腔,乌司他丁干预组于大鼠建模后给予乌司他丁注射。对几组大鼠术后肺组织及胰腺组织的病理变化及相关指标差异进行分析。结果 与假手术组比较,SAP组及乌司他丁干预组TNF-α、AMY及MDA水平明显更高,乌司他丁干预组与SAP组比较,TNF-α、AMY及MDA水平明显更低(P<0.05)。SAP大鼠伴随着明显的肺损伤状况,假手术组、SAP组、乌司他丁干预组肺湿/干比值分别为3.62±0.56、6.48±0.77、4.69±0.63,血气指标PaCO₂、PaO₂分别为(31.25±1.03)mmHg、(106.52±2.03)mmHg,(48.67±2.01)mmHg、(73.57±2.44)mmHg和(35.22±1.32)mmHg、(90...     

清营汤方剂与施他宁联合治疗对重症胰腺炎患者细胞因子水平的影响

为探讨早期应用清营汤治疗重症胰腺炎患者细胞因子水平的影响。采用酶联免疫法测定其外周血清中IL 2、IL 2R及sIL 2R水平。结果显示 ,重症胰腺炎早期联用清营汤方剂组治疗 1周后 ,机体细胞因子IL 2、IL 2Rα水平迅速上升 ,sIL 2Rα下降明显 ;而常规施他宁对照组IL 2及IL 2Rα水平呈下降趋势 ,sIL 2Rα变化不明显 ,两组结果相比差异显著。早期应用清营汤治疗重症胰腺炎患者 ,有利于尽快恢复机体免疫调节功能 ,为临床今后开展对重症胰腺炎早期清营汤治疗提供了实验证据     

Level of functioning of siblings and parents of probands of varying degrees of retardation

A further analysis of already published data supports the position that retardates of low ability level less frequently have retarded siblings, retarded parents, and parents low in occupational level than do retardates higher in ability level. The analysis supports the position that there are two types of retarded individuals, persons retarded as a result of gene or chromosomal anomalies, brain injury, etc., who more frequently occur in the lower-level retardate group, and persons whose retardation represents polygenic segregation, who more frequently occur in the higher-level group.     

局部注射辛伐他汀对骨质疏松大鼠股骨髁骨小梁的改建效应

背景：局部注射具有成骨作用的辛伐他汀,可显著增加骨质疏松大鼠股骨颈及股骨髁部的骨密度及力学强度,分析局部注射辛伐他汀对股骨髁骨小梁的影响。 目的：进一步研究骨质疏松大鼠股骨内局部注射辛伐他汀对股骨髁骨小梁的影响。为将辛伐他汀应用于临床骨质疏松局部治疗提供实验基础。 方法：18只雌性SD大鼠双侧卵巢切除后3个月,制备大鼠骨质疏松模型。实验大鼠随机数字表法均分为3组,分别在实验大鼠的右侧股骨髓腔内单次注射辛伐他汀溶液5 mg、10 mg,对照组单纯注射空白载体。分别在注射后1个月处死大鼠并取材。Micro-CT扫描并定量分析骨组织形态变化。 结果与结论：给药后1个月,Micro-CT扫描结果显示,辛伐他汀治疗组的骨微结构参数如骨皮质厚度、骨小梁密度及连接率明显优于对照组。说明疏松骨骼单次注射小剂量辛伐他汀可显著促进股骨髁部骨小梁改建,改善骨骼微结构,可为强化局部、防治骨质疏松骨折的新选择进一步提供实验基础。     

石斛属民族药用植物的分类及生药学研究

中药石斛早在《神农本草经》中就被列为上品,其应用历史悠久,具有养阴生津、补肾益气、润喉护嗓、活血明目、抗癌防老等功效。本文对国内外石斛属民族药用植物的分类及生药学研究进行了综述。     

膝关节骨性关节炎的关节镜治疗体会

目的我科自2001～2005年4月对59例67膝的骨性关节炎（0A），进行关节镜检查及镜下清理术。方法镜检：膝关节骨性关节炎伴不同程度滑膜炎，滑膜皱壁粘连纤维片，关节软骨不同程度破坏。镜下清除增生滑膜，松解粘连，去除剥脱软骨、修复关节面。结果随访2月～4年，平均1.5年，术后综合评估2月～1年，优良率94％；1～2年，优良率79.2％；2年以上优良率54.8％。结论关节镜对膝关节骨性关节炎诊断能提供了比较全面的资料，并对骨性关节炎早期有良好疗效，具有创伤小、恢复快、并发症少和重复治疗等优点。     

Anatomical study of distribution of valves of the cutaneous veins of adult''s limbs

Since the cutaneous veins of the four limbs have been used as autogenous grafts in the treatment of cardiovascular disorders, knowledge of distribution of the valves is increasingly required depending upon the use. In the gross anatomical study of distribution of valves of the trunci venae, there has been argument about locational relationships between the venous roots and the valves in the vicinity of the roots and the inter-valvular distance. However, there have been only few reports discussing detailed information about valves of the cutaneous veins of the four limbs. The authors observed patterns of distribution of the cutaneous venous valves of the four limbs of cadavers prepared for practice in anatomy. The following parts were excised from each cadaver: the cephalic, basilic, and the great saphenous veins, which originate from the acral venous network and flow into the proximal deep veins, and the venous roots communicating with these veins. An incision was made on each excised vein in the direction of the long axis under observation with a stereoscopic microscope, and the inter-valvular distance and the distance between the valve and the orifice of venous root in the vicinity of the valve were measured. The inter-valvular distance varied with type of the truncus venae, and it varied according to area even in the same truncus venae.     

Pathways of the realization of immunomodulating effects of neurotransmitters

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 115, N ^o 4, pp. 401–404, April, 1993     

Characterization of sterols of three digenetic trematodes of buffalo

Sterols of three digenetic trematodes were isolated and characterized by infrared and 1H nuclear magnetic resonance spectroscopies, and gas-liquid chromatography and gas chromatography-mass spectrometry. Sterols identified were cholesterol, cholestanol, 24-methylcholesterol, 24-methylcholestanol, 24-ethyl-22-dehydrocholesterol, 24-ethyl-22-dehydrocholestanol, 24-ethylcholesterol and 24-ethylcholestanol.     

Mechanisms of generation of slow components of bioelectric activity in diagnostics of functional state of the gastrointestinal tract

New electrophysiologic devices were used for the diagnostics of the state of excitable structures in the gastrointestinal tract and correction of their motor functions. Bioelectrical and biomechanical activities form the basis of functioning of internal organs. The mechanisms of generation of slow bioelectrical activity that are important for clinical and physiological studies are described. One of these mechanisms is a capacitance parametric transducer converting the energy of contractions into specific electric signals reflecting muscle functions. Another mechanism results from slow oscillations of resting potentials of interrelated excitable cells in large neuromuscular structures of internal organs. The elaborated procedure is efficient for preventing early postoperative paresis of the gastrointestinal tract. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 128, No. 10, pp. 448–452, October, 1999     

胃小弯侧血管弓的MDCT解剖

目的：研究正常胃小弯侧血管弓及其属支血管的多排螺旋CT表现。方法：回顾性分析51例正常成人的上腹部CT资料,观察胃小弯侧血管弓及其属支血管的显示情况、血管弓形态及其与胃壁的关系。结果：51例均显示胃小弯侧血管弓,血管弓贲门部属支、胃体部属支和幽门部属支分别显示46例、15例和10例;血管弓与胃壁紧邻41例。结论：MDCT检查可良好显示正常胃小弯侧血管弓及其属支血管。     

Assessment of the efficacy of treatment of hemangiomas

Assessment of the aftereffects of cryoexposure and ultrahigh-frequency cryoexposure on hemangioma tissue of various types, cavernous and squamous, showed a higher cryogenic effect in hemangiomatous tissue preexposed to ultrahigh-frequency waves. A quantitative criterion is proposed for assessing the efficacy of the studied methods of exposure. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 6, pp. 669–672, June, 1995