组织工程材料PLGA与组织细胞生物相容性的体内实验研究

目的探讨生物可降解材料乙交酯．丙交酯共聚物(HLGA)支架与组织细胞在体内环境下的生物相容性。方法体外培养许旺细胞(SC)和神经干细胞(NSC),1周后置于PLGA支架内并移植入大鼠T9水平脊髓半横断损伤处,不同时间扫描及透射电镜下观察支架的降解及细胞的生长状况。结果扫描电镜观察髓内移植后随时间的延长,材料逐步降解。透射电镜观察材料内见再生的神经纤维、纤维细胞、纤维母细胞和巨噬细胞吞噬坏死变性的SC。结论PLGA支架与组织细胞在大鼠脊髓内具有良好的生物相容性,这为我们下一步的实验研究打下了坚实的基础。     

猪脂肪干细胞与脱细胞真皮基质的生物相容性

背景：脱细胞真皮基质已广泛应用于器官或组织缺损的修补。 目的：观察猪脂肪源性干细胞与脱细胞真皮基质的相容性。 方法：酶消化法原代培养猪脂肪源性干细胞,利用流式细胞仪和多向诱导分化方法鉴定脂肪干细胞,将其与脱细胞真皮基质共培养。 结果与结论：实验成功培养出猪脂肪源性干细胞,流式细胞仪检测结果显示阳性表达CD44和CD105,不表达CD34和CD45;在诱导培养基条件下,可向脂肪细胞、成骨细胞分化。苏木精-伊红染色及扫描电镜发现干细胞能在脱细胞真皮基质表面黏附生长。脂肪源性干细胞与脱细胞真皮基质具有良好的相容性,将两者体外复合培养后,有望将复合物植入体内进行缺损组织、器官的修复。     

高分子组织工程材料在组织工程中的应用*○

学术背景：组织工程学的发展为组织或器官的修复与再建提供了可能,组织工程及高分子材料的研究进展值得探讨。 目的：从生物相容性的角度出发论述了组织工程的研究内容及高分子组织工程材料在组织工程中的应用。 检索策略：由该论文的研究人员应用计算机检索Pubmed数据库1990-01/2007-12相关文献,检索词"tissue engineering,tissue engineering materials,Polymers materials,bio-compatibility,bio-compatibility materials,cell-compatibility,cell-compatibility materials",并限定文章语言种类为English,同时计算机检索万方数据库1990-01/2007-12期间的相关文章,检索词为“组织工程;组织工程材料;高分子材料;生物相容性材料;生物相容性;细胞相容性;组织相容性”,并限定文章语言种类为中文。共收集到81篇相关文献,对资料进行初审。纳入标准：文章内容应与生物相容性组织工程材料相关。排除标准：重复研究或Meta分析类文章。30篇文献符合纳入标准,排除的51篇文献为内容陈旧或重复。符合纳入标准的30篇文献中,19篇涉及生物相容性,11篇涉及细胞相容性材料。 文献评价：文献的来源主要是Pubmed数据库及万方数据库。共得到论著类文章25篇,综述类文章5篇。 资料综合：组织工程研究的内容包括种子细胞种植、生物材料植入及细胞移植。细胞的研究包括基因重组技术,将同种、自体或异种的组织体外分解成细胞后培养、增殖后进行构建。材料的研究主要集中于如何将材料与活细胞建成组织工程构建,即具有生物功能的活性材料,由于组织工程材料应具备最佳的材料与细胞界面反应效果,因此设计具有化学分子水平、三维分子水平的细胞/材料杂化界面,具有宏观三维分子水平、符合生物力学要求的装置是组织工程材料研究的核心。高分子材料由于具有良好的物理机械性能、分子结构更接近于生物体而广泛用作生物材料,并在组织工程领域发挥着重要作用。 结论：研究开发具有良好组织相容性的材料是组织工程发展的基石,高分子材料具有较好的性能及接近于生物体的分子结构在组织工程中应用广泛。     

前脂肪细胞在小肠黏膜下层的生长与增殖

背景：研究表明前脂肪细胞能定向分化为脂肪细胞,具有很强的增殖能力。小肠黏膜下层具有良好的生物相容性,是理想的组织工程支架材料。 目的：将体外培养的前脂肪细胞与小肠黏膜下层复合,观察前脂肪细胞在小肠黏膜下层上的生长和增殖情况。 方法：将体外培养的前脂肪细胞接种在小肠黏膜下层上作为材料组,设置在孔板内培养的细胞作为对照组,在不同时间点用MTT法检测前脂肪细胞的相对数量并绘制生长曲线,观察前脂肪细胞在不同培养条件下的增殖能力。流式细胞仪检查两组前脂肪细胞的增殖和凋亡情况,并观察不同时间点前脂肪细胞生长和黏附情况。 结果与结论：从生长曲线可以看出,接种13 d后小肠黏膜下层上的前脂肪细胞数高于孔板内培养的前脂肪细胞数(P < 0.05),具有良好的增殖能力。小肠黏膜下层上的细胞比孔板培养的细胞有更强的增殖能力(P < 0.05),两组前脂肪细胞均无明显凋亡。由此可知,前脂肪细胞在小肠黏膜下层具有良好黏附和生长的能力。小肠黏膜下层与前脂肪细胞有良好的生物相容性。 关键词：前脂肪细胞;小肠黏膜下层;细胞增殖;生长;黏附;组织工程;生物相容性 doi:10.3969/j.issn.1673-8225.2010.25.010     

携带神经干细胞肌基膜管组织工程支架中神经干细胞的存活分化

摘要 背景：多项研究已证实神经干细胞能促进脊髓损伤大鼠神经功能的恢复,肌基膜管具有良好的细胞、组织相容性和降解性,那么能否将二者结合起来构建一个新的神经组织工程支架？ 目的：以神经干细胞为种子细胞,以肌基膜管为支架,观察携带神经干细胞的肌基膜管组织工程支架中神经干细胞的存活与分化情况。 方法：体外分离培养大鼠神经干细胞,并进行鉴定。用化学萃取方法制作去细胞骨骼肌基膜管支架,将神经干细胞移植入肌基膜管支架培养7 d后,用免疫组织化学方法检测神经干细胞的存活及分化情况,扫描电镜观察其超微结构。 结果与结论：神经干细胞分离培养第5天,Nestin免疫荧光染色可见大量神经球。加血清诱导神经干细胞分化至7 d,进行抗NF、抗GFAP免疫荧光染色,镜下可见NF、GFAP阳性细胞,证明培养的神经干细胞具有多项分化潜能。苏木精-伊红染色法显示肌基膜管中肌细胞成分已消失,肌基膜管支架内主要是大致平行的管道。携带神经干细胞的肌基膜管组织工程支架免疫荧光染色证明,神经干细胞在支架内仍具有干细胞特性,并可分化为神经元和神经胶质细胞。扫描电镜显示神经干细胞可以稳固地贴附在肌基膜管内,提示制备的神经组织工程支架具有良好的生物相容性,可以进行体内移植治疗脊髓损伤等神经系统疾病。 关键词：肌基膜管;组织工程支架;神经干细胞;移植;脊髓损伤 doi:10.3969/j.issn.1673-8225.2010.47.003     

透明质酸修饰壳聚糖复合支架在大鼠脑皮质损伤修复中的作用

背景：为了验证透明质酸修饰后的壳聚糖材料能否作为神经支架材料,课题组在前期体外实验结果的延续下,进行体内试验观察。 目的：比较经透明质酸、多聚赖氨酸修饰的壳聚糖支架及单纯壳聚糖支架在脑组织中的生物相容性及抑制瘢痕作用的优越性。 方法：将3种支架植入Wistar大鼠脑皮质损伤区,术后3,7,14,28,56 d分别取脑组织行苏木精-伊红染色、免疫组化观察,观察移植部位炎症反应及瘢痕组织形成、神经细胞生长情况。 结果与结论：各组支架与脑组织相容性良好,用透明质酸修饰的壳聚糖复合支架组与其他壳聚糖支架组相比,支架周围炎症反应轻、支架周围GFAP阳性细胞数少,差异均具有显著性意义(P < 0.01)。结果提示各组壳聚糖支架均具有良好的生物相容性,用透明质酸修饰的壳聚糖复合支架较其他壳聚糖支架抑制瘢痕作用明显,有利于神经细胞的生长,在组织工程应用中更具有优越性。 关键词：透明质酸;壳聚糖;复合支架;组织相容性;神经支架材料 doi:10.3969/j.issn.1673-8225.2010.29.009     

纤维蛋白在组织工程载体构建方面的应用

随着组织工程领域研究热潮的不断兴起，越来越多的生物材料正作为细胞、生物活性物质的载体而发挥作用，选择一合适的生物材料以作为载体是核心问题。纤维蛋白因具有良好的生物学功能和理化性质等一系列优点而被广泛应用，涉及面涵盖支架载体、复合载体以及释放载体，同时载体构建的技术水平与日俱增。文章就纤维蛋白的起源、结构、生物学功能、在组织工程中作为载体的研究进展作一综述，为纤维蛋白在组织工程中的发展提供理论依据。     

人脐血中分离培养扩增内皮集落形成细胞及其生物相容性*☆

背景：内皮集落形成细胞是内皮祖细胞的一种亚型,体外培养扩增及其在组织工程中的应用尚不明确。 目的：探索从人脐血中分离培养扩增内皮集落形成细胞并观察其生物相容性。 方法：采用密度梯度离心法从人脐血中分离单个核细胞,接种于Ⅰ型鼠尾胶原包被的培养板上,采用内皮祖细胞专用EGM-2培养基诱导培养,早期传代后扩增,免疫荧光鉴定细胞,并检测其体外成血管和增殖能力,与纳米羟基磷灰石/磷酸三钙材料复合培养后的生物相容性。 结果与结论：脐血来源内皮集落形成细胞呈铺路石样集落生长,吞噬Dil-Ac-LDL并结合FITC-UEA-1,表达CD31,vWF,KDR和Tie-2,早期传代后拥有较强增殖潜能,可在体外大量扩增,在体外基质胶上可形成闭合管腔样结构,复合纳米羟基磷灰石/磷酸三钙材料后生物学特性不受影响。提示可从脐血中分离并体外大量扩增内皮集落形成细胞。     

大鼠成骨细胞的原代培养和鉴定

背景：组织工程需要大量的种子细胞,成骨细胞已成为骨组织工程构建的重要种子细胞之一。但成骨细胞取材困难,获得的成骨细胞的纯度不一。 目的：建立大鼠新生乳鼠颅骨来源成骨细胞的分离培养纯化方法,观察颅骨来源成骨细胞生物学特点。 方法：采用二次酶消化法对SD大鼠乳鼠成骨细胞进行原代培养,扩增。通过差速贴壁法进行成骨细胞纯化。通过形态学、细胞碱性磷酸酶检测、茜素红染色、钙结节Von kossa法染色、超微结构以及细胞增殖曲线,确定其增殖与成骨活性。 结果与结论：二次酶消化法培养颅骨来源成骨细胞可获得原代细胞增殖,传代扩增细胞具有典型成骨细胞形态学和生物学活性。碱性磷酸酶、茜素红染色、钙结节Von kossa法染色均呈阳性结果。超微结构显示为高分化功能活跃成骨细胞,细胞增殖曲线显示细胞生长活跃。提示,新生SD大鼠颅骨来源成骨细胞具有良好的增殖与成骨活性,能连续传代增殖,纯度高,细胞生物学特征稳定,适用于做体外实验研究。     

Tissue expression of steroid hormone receptors is associated with differential immune responsiveness

Glucocorticoids have been used as treatments against a number of diseases, especially autoimmune/inflammatory conditions in which the immune system is overactive. These treatments have varying degrees of responsiveness among individuals and in different tissues (including brain); therefore, it is important to determine what could account for these differences. In this study, we evaluated expression of stress hormone receptors in immune cells from lymphoid and non-lymphoid tissues (including brain) as a possible explanation. We analyzed leukocytes (CD45⁺) in kidney, liver, spleen, and thymus tissues from healthy mice for expression of the receptor for stress hormone (glucocorticoid—GR) as well as other steroid hormones (androgen—AR, progesterone—PR) and found that all tissues expressed these steroid hormone receptors but with varying patterns. To determine whether tissue-specific differences were related to immune cell composition, we examined steroid hormone receptor expression in T lymphocytes from each of these tissues and found similar patterns of expression in these cells regardless of tissue source. Because glucocorticoids can also impact brain function, we further examined expression of the stress hormone receptor in brain tissue and found GR expressed in immune cells at this site. In order to investigate the potential impact in an area of neuropathology, we utilized a mouse model of West Nile Virus (WNV). We observed pathological changes in brains of WNV-infected animals and T lymphocytes in the areas of inflammation; however, these cells did not express GR. These data indicate that tissue-specific differences in steroid hormone receptor expression by immune cells could determine responsiveness to steroid hormone treatment.     

壳聚糖及其衍生物在骨组织工程中的应用*

摘要：壳聚糖是天然多糖类高分子化合物甲壳素的脱乙酰产物,具有来源丰富、生物相容性好、可生物降解等优点,在骨组织工程中可以加工成用于细胞移植和组织再生的多孔支架、生长因子的缓释载体及外源基因的递送载体,也可加工成微球或水凝胶等形式用作骨组织工程的可注射性支架,在骨组织工程研究领域壳聚糖及其衍生物具有广阔的应用前景。但由于壳聚糖复合支架的机械性能较弱、在体内的降解时间难以精确控制、壳聚糖用作基因载体转率效率偏低等原因,目前大多研究只处于体外和动物体内实验阶段,随着材料科学与生命科学的发展,壳聚糖将广泛用于骨组织缺损的临床治疗实践中。 关键词：壳聚糖;衍生物;骨;组织工程;综述文献 doi:10.3969/j.issn.1673-8225.2009.47.024     

Axon guidance in muscleless chick wings: the role of muscle cells in motoneuronal pathway selection and muscle nerve formation

The role of myogenic cells in accurate pathway selection and muscle nerve formation was studied in chick embryos. Myotubes were eliminated from the forelimb of the chick embryo by extirpating the somites, which give rise to myogenic cells. Other elements of the wing tissue, connective tissue, and cartilagenous elements derived from the somatopleure, were left intact. Injections of WGA-HRP were made into either dorsal or ventral nerve trunks in the wing and the positions of retrogradely labeled motoneurons determined. The positions of the motoneurons within the brachial lateral motor column were appropriate for the injection made. Thus, the accuracy of the motoneuronal projections was unaffected by the absence of muscle cells. The absence of myotubes was not correlated with the absence of muscle nerves. Muscle nerves were consistently observed in muscleless wings until stage 36, the oldest stage examined. Muscle nerves in muscleless wings differed from those in normal wings in that they were smooth and stubby, and lacked the normal pattern of intramuscular nerve branches. From these studies we conclude that muscle cells are not necessary for accurate motor axon guidance into the periphery along the routes of major nerve trunks, nor for the formation of muscle nerves. By inference, somatopleural derivatives provide sufficient cues for selection of specific axonal pathways and for patterning of muscle nerves within the chick limb.     

丝蛋白应用于牙周组织工程的可行性

背景：丝蛋白是有利于表皮细胞、成纤维细胞、成骨细胞、血管内皮细胞、胶质细胞黏附和生长的一种新型生物材料。 目的：评估丝蛋白作为支架材料应用于牙周组织工程的可行性。 方法：采用组织块法培养人牙周膜细胞，将第5代细胞悬液以2×107 L-1的浓度接种到丝蛋白支架材料上复合培养，并以1%，10%，50%，100%的丝蛋白支架浸提液培养，观察人牙周膜细胞在丝蛋白上及在丝蛋白浸提液中生长状况，用MTT法测定浸提液培养人牙周膜细胞的活力。 结果与结论：扫描电镜可见人牙周膜细胞在丝蛋白支架上伸展充分，生长旺盛，不同浓度丝蛋白支架浸提液培养对人牙周膜细胞的增殖与碱性磷酸酶活性均无影响。说明丝蛋白材料具有良好的生物相容性、独特的力学性能，可作为人牙周膜细胞黏附生长的理想支架材料较好地应用于牙周组织工程中。     

尿路组织工程材料的血管化*☆

学术背景：组织材料血管化是组织工程学中重要的组成部分之一,复合材料的血管化对复合其上的细胞存活以及原有组织或器官功能的恢复起到至关重要的作用。 目的：结合近年来的相关文献,对尿路组织工程修复重建中血管化的研究做一综述。 检索策略：由第一作者应用计算机检索PUBMED,中国期刊数据库 2002-07/2007-10相关文章,检索词为“tissue engineering, vascularization, angiogenesis, omentum, growth factor, endothelial progenitor cell, urethra,bladder,urology”或“组织工程,血管化,尿道,膀胱,生长因子,网膜,血管内皮组细胞,尿路”,文章语言种类为“English”或中文。纳入标准：内容应与泌尿系统组织修复重建材料血管化的研究相关。排除标准：重复文章。 文献评价：共收集到214篇相关文献,82篇符合纳入标准,其中31篇涉及网膜在泌尿系统血管化中的应用,25篇涉及生长因子在泌尿系统组织工程重建中的研究,剩余26篇为种子细胞在泌尿系统血管化中的应用。选用具有代表性的30篇。 资料综合：目前已有研究利用网膜作为生物反应器对不同的支架材料进行包埋以重建输尿管和膀胱组织,并取得了满意的效果;还有利用血管内皮细胞生长因子、碱性成纤维细胞生长因子等相关生长因子与脱细胞基质进行复合促进了毛细血管的增生的研究;更有学者利用各种相关的种子细胞与支架材料复合构建具有微血管结构的替代材料。而从干细胞和相关祖细胞上对组织材料进行微血管化的构建将是泌尿系统组织替代材料血管化的发展方向。 结论： 利用多种手段进行材料血管化的研究是目前泌尿系统组织工程研究中的重点之一,但其研究尚处在起步阶段,多数报道仅处于实验室阶段,真正应用于临床的文献较少见。因而目前来说仍有许多关键点有待于解决。     

不同年龄段大鼠骨髓基质干细胞生物学特性的比较

背景：骨髓基质干细胞取材方便，增殖力强，具有多向分化潜能，且可自体移植，是组织工程中理想的种子细胞。年龄等因素对骨髓基质干细胞生物学特性的影响鲜见报道。 目的：比较不同年龄段大鼠骨髓基质干细胞生物学特性的差异，为临床细胞移植提供实验依据。 方法：骨髓基质干细胞供体为SD大鼠。按细胞来源分为3组，即幼年组、成年组和老年组。无菌条件下剪断大鼠股骨两端，用含体积分数为10%胎牛血清的DMEM培养液冲洗骨髓腔，收集骨髓冲洗液，吹打制成单细胞悬液，通过200目的筛网滤过后将其置于培养瓶中培养。取第2代生长均一的细胞，CD44，CD29，CD90 免疫荧光鉴定；观察细胞形态及黏附速度；细胞计数，绘制生长曲线；MTT法检测3组细胞的增殖活力。 结果与结论：CD44，CD29，CD90免疫染色细胞呈阳性；幼年组骨髓基质干细胞的黏附速度快于成年组和老年组；细胞增殖幅度明显 (P < 0.05)；MTT法测定结果显示幼年组骨髓基质干细胞活力强，与另外两组比较差异具有显著性意义(P < 0.05)。提示幼年SD大鼠骨髓基质干细胞增殖快、活力强、可在短时间内大量扩增，是理想的组织工程细胞。     

Preparation and biological evaluation of chitosan–collagen–icariin composite scaffolds for neuronal regeneration

In this study the chitosan/collagen/icariin composite scaffolds for nerve regeneration were produced by blending and crosslinking chitosan with collagen and icariin. The microstructure of the composite scaffolds was observed by scanning electron microscopy. 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide and attachment assays were conducted, respectively, to investigate the biocompatibility of the scaffolds. Cell cultural tests showed that the channel-structured porous scaffolds acted as a positive factor to support connective nerve cell growth. After culture, cells showed a clear flow trend to move close to the composite scaffolds in culture solution, arranging in spiral-like, and aligned parallel to the orientation of the channel structure on the surface of scaffolds. When compared to pure chitosan and chitosan/collagen scaffolds, Schwann cells and PC12 cells on the chitosan/collagen/icariin composite scaffolds exhibited the greatest proliferation and longest average neurite length. These results suggested that the chitosan/collagen/icariin composite scaffolds are potential cell carriers in nerve tissue engineering.     

多种生物材料细胞生物相容性及其安全性的系统评价

OBJECTIVE: To discuss the biosafety and biocompatibility of biomaterials. METHODS: A computer-based online search of CNKI, CBM and VIP as well as manual search were performed to collect articles about random cell control experiments and animal experiments of biological material biocompatibility published between 1990 and 2008. A total of 28 Chinese literatures were collected, and 7 were included mainly involving the cytotoxicity test methods and blood compatibility of the experimental medium, experimental grouping, experimental materials, methods of observation, experimental results, and experimental conclusion. In addition, biocompatibility of the biological material was analyzed to summarize the biocompatibility of the biological safety. RESULTS: Based on experiments of biosafety and biocompatibility of biomaterials, the cytotoxicity test and blood compatibility of various biomaterials, such as collagen, chitosan, magnetic nanoparticle, metal vascular stent, sulphurated siliastic medical grade silicon rubber, polyurethane, ceramic coatings by micro-arc oxidation, are essential for the biological safety. The experimental results have shown that the biomaterials have good biocompatibility. CONCLUSION: A variety of biological materials present good biocompatibility, including collagen, chitosan, magnetic nanoparticle, metal vascular stent, sulphurated siliastic medical grade silicon rubber, polyurethane, ceramic coatings by micro-arc oxidation, based on the evaluation criteria.     

Implantation of a new porous gelatin-siloxane hybrid into a brain lesion as a potential scaffold for tissue regeneration.

For brain tissue regeneration, any scaffold for migrated or transplanted stem cells with supportive angiogenesis is important once necrotic brain tissue has formed a cavity after injury such as cerebral ischemia. In this study, a new porous gelatin-siloxane hybrid derived from the integration of gelatin and 3-(glycidoxypropyl) trimethoxysilane was implanted as a three-dimensional scaffold into a defect of the cerebral cortex. The porous hybrid implanted into the lesion remained at the same site for 60 days, kept integrity of the brain shape, and attached well to the surrounding brain tissues. Marginal cavities of the scaffolds were occupied by newly formed tissue in the brain, where newly produced vascular endothelial, astroglial, and microglial cells were found with bromodeoxyuridine double positivity, and the numbers of those cells were dose-dependently increased with the addition of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Extension of dendrites was also found from the surrounding cerebral cortex to the newly formed tissue, especially with the addition of bFGF and EGF. The present study showed that a new porous gelatin-siloxane hybrid had biocompatibility after implantation into a lesion of the central nervous system, and thus provided a potential scaffold for cell migration, angiogenesis and dendrite elongation with dose-dependent effects of additive bFGF and EGF.     

Survival of neural precursor cells in growth factor-poor environment: implications for transplantation in chronic disease

A key issue for therapeutic neural stem cell transplantation in chronic diseases is the long-term survival of transplanted cells in the brain. The normal adult central nervous system does not support the survival of transplanted cells. Presumably, the limited availability of trophic factors maintains the survival of resident cells but is insufficient for supporting the survival of transplanted cells. Specifically, in multiple sclerosis, a chronic relapsing disease, it would be necessary to maintain long-term survival of transplanted cells through phases of relapses and remissions. It may be beneficial to transplant cells as early as possible, in a form that will keep their survival independent of tissue support and ready for immediate mobilization upon tissue demand during disease relapse. In the present study, we examined whether, in the form of neurospheres, multipotential neural precursor cells (NPCs) survive in a growth factor-poor environment while maintaining their potential to respond to environmental cues. We found that after removal of growth factors from the culture medium of neurospheres in vitro, NPC proliferation decreased significantly, but most cells survived for a prolonged time and maintained their stem cell characteristics. After re-exposure to growth factors, neurosphere cells resumed proliferation and could differentiate along neural lineages. Furthermore, neurospheres, but not single NPCs, that were transplanted into the brain ventricles of intact animals survived within the ventricles for at least a month and responded to induction of experimental autoimmune encephalomyelitis and brain inflammation by extensive migration into the brain white matter and differentiated into glial lineage cells.     

骨髓基质细胞体外分离培养和分化为

摘要 目的：观察体外培养大鼠骨髓基质细胞（BMSCs bone marrow stromal cells）的生物学特性并探讨其分化为Schwann细胞表达S100的机制,为组织工程学修复周围神经损伤奠定一定的实验基础。方法：从成年SD大鼠的股骨和胫骨中分离培养BMSCs,倒置显微镜下动态观察细胞的生长状态,采用MTT法检测细胞的活力,FCM检测细胞周期等方法研究BMSCs的生物学特性。应用复合诱导因子（BME, RA ,FSK, bFGF, PDGF, HRG）体外诱导BMSCs向Schwann细胞分化。诱导分化后采用免疫荧光细胞化学染色,流式细胞仪,逆转录PCR方法分别检测检测胶质细胞标记蛋白S100蛋白和mRNA的表达。结果：BMSCs在体外容易扩增,传1－8代以内的细胞增殖能力无明显变化。未经诱导的BMSCs大部分处于G0和G1期。诱导分化后的细胞形态上类似Schwann细胞,并且表达胶质细胞的标记蛋白S100蛋白及其mRNA明显升高。结论：BMSCs在体外可以向类Schwann细胞诱导分化,并且表达胶质细胞的标记蛋白S-100蛋白及其mRNA。