急性髓细胞白血病树突状细胞的体外定向诱导及鉴定

目的 :从急性髓细胞白血病 (AML)外周血细胞诱导产生树突状细胞。方法 :将AML外周血细胞做原代培养 ,培养过程中加入GM -CSF、IL - 4和TNF -α等三种细胞因子诱导细胞向DC分化 ,并运用形态和免疫表型特点鉴定细胞。结果 :通过形态和免疫表型鉴定均证实 ,培养后多数细胞均符合DC特点 ,培养后细胞CD83和CD86共同表达的阳性率为 5 2 7% ,CD1a和HLA -DR共同表达阳性率为 17 1% ,外周血 1× 10 6个MNC经培养后可产生约 (6～ 11)× 10 4个DC。结论 :体外培养的AML患者外周血单个核细胞可诱导分化出大量DC     

钙离子载体促进K562细胞诱导分化成DC样细胞的研究

目的 观察钙离子载体(CI)诱导慢性髓系白血病细胞株K562分化成DC样细胞的作用.方法 将细胞分三组培养:第1组加入GM-CSF 1 000 U/ml和IL-4 500 U/ml;第2组加入GM-CSF 1 000 U/ml、IL-4 500 U/ml 和CI 375 ng/ml;第3组为对照,不加细胞因子,也不加CI.流式细胞仪检测各组细胞表面免疫表型的表达,MTT 比色法检测其刺激同种异体T 细胞增殖的作用.结果 细胞因子加CI 组培养48 h后即可见细胞形态呈多形性,有些细胞可见突起;96 h后CD80、CD86、CD83的表达率较对照组、单用细胞因子明显提高,明显刺激同种异体T 淋巴细胞增殖.单用细胞因子组培养96 h后细胞形态无明显变化,CD80、CD86的阳性率明显高于对照组,CD83与对照组之间差异无统计学意义,不能明显刺激同种异体T 淋巴细胞增殖.结论 CI与GM -CSF和IL-4联合可迅速将K562细胞诱导成DC样细胞;CI与细胞因子可能有协同作用.     

细胞因子体外诱导急性髓系白血病细胞分化为树突状细胞

目的　研究细胞因子组合体外诱导急性髓系白血病 (AML)细胞分化为树突状细胞 (DC)的可行性及AML细胞衍生DC(AML DC)的生物学特性。方法　AML细胞分别在GM CSF +IL 4、GM CSF +TNF α或GM CSF +IL 4 +TNF α 3种细胞因子组合以及不含细胞因子的培养液中培养。通过细胞形态动态观察、细胞化学染色和细胞免疫表型鉴定DC。用混合淋巴细胞培养 (MLC)、FITC标记的葡聚糖摄入实验和LDH释放实验检测DC功能。RT PCR和FISH检测AML DC的特异融合基因。结果　15例AML细胞在 3种细胞因子作用下发生典型的DC形态变化。AML DC的DC相关表面分子CD1a、CD80 、CD86 、CD1 0 6 、CD83和HLA DR等较未培养或不加细胞因子培养的AML细胞表达明显上调 (P <0 0 5 )。AML DC的异基因刺激能力明显高于未培养或不加细胞因子培养的AML细胞 (P <0 .0 5 )。只有用GM CSF +IL 4培养的AML DC有吞噬能力。AML DC与未培养AML细胞致敏的疾病初发时分离的T细胞比较 ,对自体AML细胞无明显的杀伤活性。AML DC仍具有未培养AML细胞的特异融合基因。结论　体外细胞因子可诱导各型AML细胞分化为DC ,细胞因子组合不同 ,AML DC的成熟状态可有一定的差异。AML DC不但起源于AML细胞 ,而且具有正常DC的典型形态、表型和功能。AML细胞可导致自体T细胞失能     

全反式维甲酸诱导急性早幼粒细胞白血病细胞分化为树突状细胞的实验研究

全反式维甲酸（ATRA）诱导APL细胞分化为成熟树突状细胞（dendritic cell，DC），目前国内外鲜见报道。本研究探讨全反式维甲酸与经典细胞因子共同作用诱导急性早幼粒细胞白血病（APL）细胞分化为DC的可能性，为研制APL-DC疫苗提供新的方法。对初治APL患者骨髓单个核细胞及HL-60细胞株分别在完全培养液中培养，用经典细胞因子组合（GM—CSF、IL-4、TNF-α）共处理，ATRA只在实验组加用。观测细胞形态，检测DC免疫表型，测定上清液IL-12水平，观察MLR反应及CTL效应。结果表明：实验组所得细胞有较明显树突状突起，有APL遗传学特征，其CD1a、CD83、CD80、CD86、HLA—DR和CD1d表达及IL-12分泌水平明显增高，并具有明显的MLR反应及CTL效应，但在HL60-DC中，CD1a增加无统计学差异。结论：利用ATRA可以成功诱导APL细胞为成熟功能性DC，其介导的MLR及CTL效应明显。经ATRA组合诱导的树突状细胞CD1d表达明显增高，推测可能参与NKT细胞激活，具体机制需进一步研究。     

健康人外周血树突状细胞体外培养及表型鉴定

目的 分离人外周血单个核细胞,经体外诱导培养获取成熟树突状细胞(DC),并对树突状细胞表型表达鉴定,为进一步研究DC功能提供基础.方法 取健康成人新鲜外周血,经密度梯度离心法分离获得外周血单个核细胞,2 h贴壁后加入粒细胞集落刺激因子(GM-CSF)、白介素(IL)-4,第6天加入肿瘤坏死因子-α(TNF-α)刺激DC成熟,倒置显微镜观察每日细胞形态;分别于第1天、第6天、第8天用流式细胞仪对其进行表型鉴定;同种异体混合淋巴细胞反应观察对T细胞的抗原提呈能力 倒置显微镜下DC细胞形态不规则,表面有毛刺状突起,呈DC典型形态学特征;成熟DC细胞表面CD1a、CD80、CD83、人类白细胞抗原(HLA)-DR表达明显增加,混合淋巴细胞反应OD值增加.结论 用GM-CSF、IL-4和TNF-α可以诱导健康成人外周血单个核细胞,培养出成熟的DC细胞,为进一步研究DC功能提供基础.     

急性呼吸综合征患者外周血树突状细胞表型和功能的变化

目的 探讨严重急性呼吸综合征病毒(SARSV)感染对树突状细胞(DC)表型和功能的影响。方法 采用流式细胞仪检测SARS发病期患者(9例)和正常人(7例)DC的表型；分离培养DC和T淋巴细胞，用混合淋巴细胞反应检测SARS患者和正常人DC对T淋巴细胞增殖的诱导作用。结果 SARSV感染抑制了DC类刺激分子CD80、CD86和HLAⅡ类分子HLA—DR以及与DC成熟有关的分子CD83的表达；抑制DC诱导T淋巴细胞增殖的能力。结论 SARSV可抑制DC的分化成熟和抗原递能力。     

无血清体外培养树突状细胞的研究

本研究的目的是建立对外周血来源的树突状细胞（dendritic cell，DC）的无血清培养方案。以含胎牛血清（FCS）、人AB血清的培养液作为对照。分离健康志愿者外周血单个核细胞（PBMNC），在不同培养液中分别加入GM—CSF（100ng／ml）、IL-4（500U／ml）培养6天，再分别加入钙离子载体A23187（100ng／ml）继续培养24小时，于倒置显微镜下观察细胞形态，流式细胞术分析细胞表型，MTT比色法检测各组DC刺激同种异体外周血T细胞增殖的能力和DC刺激的T细胞对K562细胞的杀伤作用。结果表明：无血清培养组培养出典型形态的DC，其表面CD14分子的表达明显减少，CD83、HLA—DR、CDw123分子的表达明显增高，具有明显的刺激同种异体T细胞增殖的能力和DC刺激的T细胞对K562细胞的杀伤作用。无血清组与两个含血清的对照组比较，组间无显著性差异（P〉0．05）。结论：无血清培养液培养的DC与含血清的培养液培养的DC相比，无显著性差异。DC无血清培养具有临床应用前景。     

体外诱导人脐血树突状细胞介导T细胞抗白血病的细胞毒性效应研究

为了研究脐血淋巴细胞能否在体外培养成为特异性杀伤白血病细胞的杀伤性T细胞(CTL),联合细胞因子体外诱导脐血单个核细胞分化为树突状细胞(DC),再吞噬凋亡白血病细胞并将其抗原呈递给相同脐血的T淋巴细胞,得到杀伤性T细胞。用形态学及流式细胞术检测DC。CTL细胞的杀伤功能用乳酸脱氢酶(LDH)释放法测定。结果表明:12份脐血标本均可培养出形态典型的DC,DC的表面标志CD1a 、HLA DR 、CD86 、CD83 表达水平显著升高(P<0.05)。CTL可以杀伤未经培养的白血病细胞(效∶靶=50∶1对AML细胞的平均杀伤率为44.76±17.42%,对ALL细胞的平均杀伤率为8.50±4.25%),对相同患者缓解期的骨髓细胞杀伤率极低。结论: 在外体应用多种细胞因子刺激可诱导脐血单个核细胞分化成典型的DC;负载有白血病抗原的DC可诱导同一脐血的淋巴细胞生成白血病特异的杀伤性T细胞(CTL),所得CTL可特异性杀伤未经培养的白血病细胞而不严重伤害相同患者缓解期的骨髓细胞。     

人外周血单核细胞来源的树突状细胞的体外诱导

目的:探讨在体外从人外周血单核细胞诱导培养成熟的树突状细胞(dendritic cell,DC)的方法.方法:培养过程分两阶段,第一阶段:采用密度梯度离心法分离健康成人外周血中的单个核细胞,再以黏附法分离出单核细胞,经重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)100 ng/mL、重组人白介素-4(rhIL-4)100 ng/mL体外诱导.第二阶段:第5天加入重组人肿瘤坏死因α-(rhTNF-α)100 ng/mL,继续培养2 d,刺激DC成熟.倒置显微镜下观察DC形态,流式细胞仪检测DC表面标志物CD83、CD1a、CD86、CD40、CD14表达水平,用MTT法测定DC刺激同种异体T细胞增殖的能力.结果:人外周血单核细胞经rhGM-CSF及rhIL-4诱导培养5 d后,多数细胞呈集落生长,细胞表型CD83、CD1a、CD86、CD40及CD14分别是14.3%、12.8%、20.1%、19.9%及16.2%.加入rhTNF-α诱导后,即培养第7天,细胞表型CD83、CD1a、CD86、CD40及CD14分别是29.8%、18.2%、33.6%、28.1%及8.0%(与第5天比较,均P<0.05).成熟后的DC具备较强刺激T细胞增殖的能力.结论:rhGM-CSF联合rhlL-4诱导人外周血单核细胞,可获得大量不成熟的DC,该体系有利于Dc扩增,加入rhTNF-α,继续培养2 d,可诱导出成熟的DC,成熟的DC具备较强刺激T细胞增殖的能力.     

细胞因子体外诱导慢性髓细胞性白血病树突状细胞分化的研究

本研究探讨干扰素（IFN）治疗慢性粒细胞性白血病（CML）的可能新机制，为临床治疗CML提供新的思路。用Ficoll密度离心法分离骨髓单个核细胞（BMMNC），用不同细胞因子组合体外诱导培养树突状细胞（DC），在倒置显微镜及光镜下观察DC形态，应用流式细胞术检测其表面标志（CD1a，CD83，CD86，HIA-ABC，HIA-DR，CD54）。MTT法检测其混合淋巴细胞反应（MLR）能力。结果表明：经不同细胞因子组合所诱导出的DC，其特征性表面分子表达率均高于培养前的DC（P〈0．01）；IFN-α＋GM-CSF组DC表达HLA—ABC、HLA-DR明显高于IL-4＋GM-CSF培养组（P〈0．05）；而IFN-α＋GM-CSF4-IL-4组DC的CD86表达率及MLR水平均高于其它细胞因子组合组（P〈0．05）。干扰素耐药组DC表面分子表达率及MLR水平较新诊断组和干扰素治疗有效组均明显减低（P〈0．05），但A、B、C各组的CD86表达率及MLR水平在IFN-α＋GM-CSF4-IL-4因子组合条件下无明显差异。结论：CML骨髓单个核细胞在含有IFN-α的细胞因子组合条件下可分化为具有形态和免疫表型特征的DC，且表达较高的Ⅰ类和Ⅱ类分子、共刺激分子、黏附分子及MLR，表明干扰素治疗CML的机理可能与DC有关，这一结果提示通过增强内源性DC功能可能提高CML临床疗效。     

IL-10对培养的树突状细胞影响的研究

目的:探讨IL-10对培养的树突状细胞的影响。方法：采用Ficoll—Hypaque淋巴细胞分离液从正常人外周血分离PBMC，以细胞贴壁法分离得单核细胞。在RPMI 1640培养液中加入rhGM—CSF和rhIL-4诱生树突状细胞(DC)，分别在培养第8，第13d时加入0，12．5，25，50，100ng／ml浓度梯度的IL-10。作用2d后，用倒置相差显微镜或荧光显微镜观察细胞形态，流式细胞术分析DC表型。结果：IL-10对成熟DC的表型表达无影响；IL-10抑制未成熟DC的共刺激分子CD86和DC成熟特异标志CDla，CD83的表达，对CD80的表达没有影响；对未成熟DC的Ⅱ类抗原HLA—DR的表达率无影响，但使其表达的平均荧光强度降低；IL-10能使未成熟的DC向耐受原性抗原提呈细胞(APC)转化。结论：为过敏性疾病和自身免疫性疾病的预防和治疗提供了实验依据。     

Optimization of the concentration of autologous serum for generation of leukemic dendritic cells from acute myeloid leukemic cells for clinical immunotherapy

Clinical application of immunotherapy for acute myeloid leukemia (AML) requires the efficient induction of dendritic cells (DCs) from AML blast cells using in vitro culture. We examined the effect of autologous serum on the properties of leukemic DCs derived from leukemic cells of AML patients by culture in AIM-V medium with GM-CSF, IL-4, TNF-alpha, and 0, 2, 5, or 10% human autologous serum. The expressions of CD80, CD83, CD86, and HLA-DR were upregulated under all culture conditions; however, 10% autologous serum induced the highest expression levels of several molecules. The capacity of leukemic DCs to stimulate allogeneic T cells increased with increasing serum concentration. Stimulation of autologous CD3(+) T cells with leukemic DCs grown in the presence of various concentrations of autologous serum resulted in induction of more IFN-gamma-secreting cells than was the case for unprimed CD3(+) T cells. Leukemic DCs cultured with 10% autologous serum induced the highest numbers of IFN-gamma-secreting cells and CD8(+)CD56(+) T cells from autologous T cells. These results suggest that culture of AML blast cells in the presence of autologous serum could be used to generate leukemic DCs for immunotherapy against AML. The highest serum concentration appeared optimal for generating the most potent leukemic DCs.     

The generation of leukemic dendritic cells from acute myeloid leukemia cells is potentiated by the addition of CD40L at the terminal maturation stage

Leukemic dendritic cells (DCs) that are derived from acute myeloid leukemia (AML) cells display low-level expression of several key molecules. We investigated the optimal combination of cytokines needed to generate potent leukemic DCs from AML cells in vitro. AML cells were cultured in the presence of the following combinations of cytokines: Group A, granulocyte-macrophage colony-stimulating factor (GM-CSF) + interleukin-4 (IL-4) + tumor necrosis factor-alpha (TNF-alpha); Group B, GM-CSF + IL-4 + CD40L; and Group C, CD40L addition at the terminal maturation point of cells that were grown as for Group A. The AML cells showed clear upregulation of CD80, CD83, CD86, CD40, and HLA-DR expression under all culture conditions, without significant differences between these groups. However, the addition of CD40L (as in Group C) showed a slight upregulation in the expression of CD83 and CD86 on leukemic DCs. The leukemic DCs in Groups A and B had higher allogeneic T-cell stimulatory capacities than untreated AML cells, and the addition of CD40L (Group C) enhanced this effect. The function of the cytotoxicity-stimulating autologous T cells was also augmented by the addition of CD40L (Group C). These results suggest that AML cells may be used to generate leukemic DCs using various cytokine combinations, and that the most potent, mature leukemic DCs are generated by the addition of CD40L to terminal-stage AML cultures that are grown in the presence of conventional cytokine combinations.     

Generation of dendritic cells from adherent cells of cord blood by culture with granulocyte-macrophage colony-stimulating factor, interleukin-4, and tumor necrosis factor-alpha

Although dendritic cells (DC) can be cultured from cord blood (CB) CD34+ progenitor cells, the generation of DC from CB monocytes has not been reported. In this paper, we explored the generation of DC from CB monocytes to establish the simplest way to obtain a substantial number of DC from CB. We isolated monocytes from CB mononuclear cells (CB-MNC) by the plastic adherence method. These adherent cells (monocyte-rich cells) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) or in serum-free X-VIVO 15 medium (SFM) for 7 days, both of which contained 100 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF) and 10 ng/ml interleukin-4 (IL-4) with or without 10 ng/ml tumor necrosis factor-alpha (TNF-alpha) (added at day 5). In the presence of GM-CSF and IL-4, CB-adherent cells became nonadherent, acquired DC morphology, and showed increased expression of CD1a, CD80, CD86, and HLA-DR; they lost membrane CD14 and some cells with the expression of CD83 and CMRF-44 were generated. With the addition of TNF-alpha to these cultures and culturing for further 2 days, the proportion of CD83+ cells was elevated in both the FBS and SFM culture systems, compared with the culture without TNF-alpha. In the culture with TNF-alpha, cells expressing CD1a, CD80, CD86, HLA-DR, and HLA-DQ were markedly increased. TNF-alpha-treated cells were demonstrated to be stronger stimulators for proliferation of both allogeneic CB lymphocytes and PB lymphocytes than were cells not treated with TNF-alpha. The yield of CD83+ DC at day 7 of cultures was 4.9 +/- 1.1 x 10(5) or 3.0 +/- 0.5 x 10(5) per 1.2 x 10(7) CB-MNC plated initially when cultured in FBS or SFM, respectively. These results have shown that a substantial number of mature DC could be generated from CB-adherent cells even by serum-free culture. We then compared these CB-adherent cell-derived DC (CB-DC) with peripheral blood (PB)-adherent cell-derived DC (PB-DC) in cell-surface phenotype and function. We found day 7 CB-DC have lower expression of CD80, CD1a, CD83, and CMRF-44 than day 7 PB-DC, but CB-DC have a similar capacity to stimulate the proliferation of both allo-CB lymphocytes and PB lymphocytes, compared with PB-DC. CB-DC cultured with GM-CSF and IL-4 have almost identical capacity of phagocytosis to take up fluorescein isothiocyanate (FITC)-dextran and Lucifer yellow (LY), compared with PB-DC. In summary, our findings suggest CB adherent cells, when cultured with GM-CSF, IL-4, and TNF-alpha, are a potent source of functional DC. Thus, CB-DC as well as PB-DC may become valuable tools for immunotherapy.     

低温保存对白血病源性树突状细胞的影响

本研究观察低温保存对白血病源性树突状细胞（L-DCs）的生物学特性和功能的影响。取急性、慢性髓系白血病骨髓细胞,将其一部分细胞立即检测,一部分细胞立即培养,一部分细胞加入细胞保护剂低温保存一定时间复温后培养。细胞培养时,在细胞培养体系内加入组合细胞因子并培养12天,然后分别检测3组细胞的细胞形态、细胞免疫表型、混合淋巴细胞反应及细胞毒T淋巴细胞（CTL）效应,并对结果进行相互比较分析。结果表明：在细胞形态学方面,急性、慢性髓系白血病患者骨髓细胞在不染色或在瑞氏染色下仅见白血病细胞,而未观察到“树突状”细胞,但经组合的细胞因子培养后,均出现了“树突状”形态的细胞,低温保存前、后二者比较,无明显差异;在细胞表面免疫标志表达方面,经组合细胞因子培养的细胞与未培养的细胞相比,细胞表面表达的CD80、CD54、HLA-DR、CD1a、CD83、CD86明显上调,而CD14表达则明显下调,低温保存前、后的细胞在上述几种细胞表面免疫表达指标方面相比较无明显差异;低温保存后的白血病细胞经培养后,不仅有明显的混合淋巴细胞反应,而且在与T细胞作用后T细胞表面抗原CD8和CD25免疫标记细胞明显增多,明显地表现了CTL杀伤自体白血病细胞的效应。结论：髓系白血病细胞在组合细胞因子培养条件下,可诱生为L-DC;L-DC的诱生在生物学特性方面不受低温保存的影响。     

γ-线照射对人树突状细胞表型及功能的影响

为了了解γ-线照射是否影响体外培养的树突状细胞的表型与功能，利用含rhGM-CSF(800U/ml)和rhIL-4(500U/ml）的RPMI1640培养基从多发性硬化症病人的外周血单个核细胞（PBMNC）中诱生树突状细胞（DC）。在培养的第6天加入5μg/ml的脂多糖继续培养24小时促使DC完全成熟。于第7天收获DC并等分成几部分，一部分未经γ-线照射的DC用作对照组，其他部分的DC分别用25Gy和30Gy剂量的γ射线照射。流式细胞术分析DC的表面分子并测定DC细胞刺激同源T细胞增殖的能力。结果表明，γ-线照射减少树突状细胞CD86，CD80和HLA-DR表达，尤其是CD86分子的表达（P=0.0072）。人DC能有效地刺激同源T细胞的增殖，但是同未经照射的DC相比，照射的DC刺激T细胞增殖的能力显降低。结论：γ-线照射不仅影响DC的表型，而且影响它的功能。     

Differential effects of autologous serum on CD34(+) or monocyte-derived dendritic cells

Dendritic cells (DC) with potentially important clinical applications have been generated from human peripheral blood monocytes and CD34(+) cells in the presence of recombinant cytokines granulocyte-macrophage colony-stimulating factor (GM-CSF) + interleukin-4 (IL-4) and GM-CSF + tumor necrosis factor-alpha (TNF-alpha), respectively. Many of the studies generating DC have included fetal calf serum, which is not desirable due to the risk of immune reactions and infectious disease transmission. Additionally, low DC yields have been reported using serum-free media. In this study, we investigate supplementing serum-free media with autologous serum and plasma for DC generation from monocytes and CD34(+) cells. Our results show that functional DC can be reproducibly obtained in the presence of autologous serum using monocytes and CD34(+) cells as the starting populations. However, with the addition of autologous serum, a differential effect is observed in the phenotypic characterization of these culture-derived DC. Monocytes cultured for 7 days in X-VIVO 15 serum-free media in the presence of GM-CSF + IL-4 showed down-regulation of CD14 with increased expression of HLA-DR, mannose receptor, CD80, and CD86, along with highly up-regulated CD1a(+) expression. The addition of autologous serum to serum-free media in monocyte cultures resulted in a dose-dependent decrease in the CD1a(+) expression generating a distinct subset of CD1a(+/-) cells expressing HLA-DR, mannose receptor, CD80, and CD86. Upon stimulation with CD40L cells, both monocyte-derived DC subsets CD1a(+/-) and CD1a(++) were capable of maturation measured by CD83 and CD86 up-regulation. Data suggest the differences in the monocyte-derived DC in serum-free (CD1a(++)) or autologous serum (CD1a(+/-)) supplemented cultures is of a qualitative nature, rather than quantitative. CD1a(+) and CD14(+) cells expressing HLA-DR, mannose receptor, CD80, and CD86 were generated in 7 days from CD34(+) cells in serum-free media. A quantitative effect was obtained when cultures were supplemented with autologous serum, resulting in a significant enhancement of CD34-derived DC generated. These results demonstrate generation of DC from two different starting populations using serum-free media that can be enhanced with the addition of autologous serum. Interestingly, a differential effect was observed in the phenotypic characterization of these culture-derived DC.     

人脐血树突状细胞体外诱导抗白血病细胞毒效应

为了体外观察树突状细胞 (DC)诱导的抗白血病细胞毒效应 ,采用细胞因子组合体外扩增培养脐血单个核细胞 (CBMNC) ,对产生的DC进行形态学观察、免疫表型检测和功能鉴定 ;用DC制备细胞毒T淋巴细胞 (CTL)和51Cr释放法测定CTL的溶靶细胞毒性。结果表明 :CBMNC中T细胞亚群与成人外周血相似 ;CD1a阳性细胞含量极少 ,仅为 (0 .4 1± 0 .0 9) % ;在DC扩增培养过程中 ,DC形态发生明显变化 ,胞体变大 ,形态不规则 ,有的细胞出现毛刺状突起 ,有的细胞出现粗大树根状突起 ;在培养 15天时 ,细胞群中CD1a阳性细胞达 (2 8.4± 3.5 5 ) % ,有 (6 3.6 7± 2 3.33) %的细胞表达CD86 ,(8.7± 1.4 9) %的细胞表达CD83,(32 .5± 1.5 3) %的细胞表达HLA DR ;产生的DC对异基因淋巴细胞具有明显的增殖刺激作用 ;经冻融的HL 6 0全细胞抗原脉冲的DC致敏自体T淋巴细胞产生的CTL对HL 6 0细胞具有特异性细胞毒效应。结论 :本研究中的脐血DC培养体系能产生较高含量的DC ,脐血DC在体外能诱导产生抗白血病细胞毒效应。