屋尘螨诱导肠道菌群失调小鼠变应性气道反应模型的建立

目的:在肠道菌群失调小鼠模型上采用屋尘螨变应原诱导气道变应性,建立一种新的变应性气道反应模型.方法:60只BALB/c小鼠分6组,2组用于肠道菌群检测;其余4组用于气道变应性检测.结果:抗生素组小鼠出现肠道菌群失调伴体重减少,粪便含水量增加.肠道菌群失调后进行屋尘螨变应原激发的小鼠BALF中细胞总数、淋巴细胞、中性粒细胞和嗜酸粒细胞增加,IL-4水平增高,肺部GATA-3转录因子mRNA表达增加.结论:用屋尘螨变应原在肠道菌群失调小鼠模型上气道激发,诱导小鼠产生Th2细胞优势的变应性气道反应.     

菌群失调小鼠全身及肠道局部免疫功能的实验研究

目的 探讨菌群失调对全身及肠道局部免疫功能的影响.方法 用林可霉素灌胃制备小鼠肠道菌群失调模型,进行肠道菌群定量分析,同时MTT法检测脾淋巴细胞增殖活性、ELISA法检测肠粘膜sIgA、血清及肠组织IL-2的含量.结果 小鼠菌群失调后,脾淋巴细胞对丝裂原的反应性降低(P<0.05),肠粘膜sIgA、血清及肠组织IL-2含量均较正常对照组低(P<0.05).结论 抗生素致肠道菌群失调后可降低机体全身及肠道局部免疫功能.     

调节小鼠肠道菌群和预防肠道菌群失调探讨

目的 建立调节小鼠肠道菌群动物模型和预防肠道菌群失调的模型,探讨微生态调节剂不同的使用方法对肠道菌群失调的作用.方法 ①预防菌群失调:建立小鼠肠道菌群失调预防组和对照组模型;②调节菌群失调:建立小鼠肠道菌群失调模型后,再分为自然恢复组和调节组.以上各组分别在给受试物后无菌采取粪便,观察各组双歧杆菌,乳酸杆菌,肠杆菌及肠球菌数量.结果 ①预防菌群失调:在菌群失调下同时给予微生态调节剂,与正常对照组相比较小鼠肠道内的双歧杆菌、乳酸杆菌的数量明显增加;②调节菌群失调:菌群失调后,给予微生态调节剂,乳酸杆菌及双歧杆菌在小鼠肠道内的数量与自然恢复组相比较明显增加.结论 微生态调节剂产生调节小鼠肠道菌群,增殖双歧杆菌和乳酸杆菌的作用.停用抗生素后肠道菌群恢复较快.     

纳米化四君子汤对免疫抑制小鼠肠道菌群的影响

目的：观察纳米化四君子汤对免疫抑制小鼠免疫功能及肠道菌群的影响,探讨神经-内分泌-代谢-微生态-免疫网络之间的关系。方法：应用氢化可的松制备小鼠免疫功能抑制模型,造成肠道菌群失调;观察四君子汤对小鼠吞噬细胞功能、外周血IL-2含量的影响和肠道菌群的调整,同时设常态中药组、自然恢复组和正常对照组进行比较。结果：氢化可的松灌喂3 d后,小鼠吞噬细胞的吞噬功能、外周血IL-2含量明显下降,肠道菌群失调;经四君子汤治疗6 d后,小鼠吞噬细胞吞噬功能、外周血IL-2含量显著提高,肠道菌群恢复正常。结论：纳米化四君子汤能显著提高免疫抑制小鼠的免疫功能、有效地调整肠道菌群失调,且功效优于常态中药组。     

基于TLR4-Ig融合蛋白探究TLR4信号在溃疡性结肠炎中的关键作用

目的 探究Toll样受体4（TLR4）表达水平与溃疡性结肠炎（UC）发生发展的关系,利用TLR4-Ig融合蛋白阻断TLR4信号,验证TLR4信号对葡聚糖硫酸钠（DSS）诱导的小鼠急性结肠炎的保护作用。 方法 收集UC患者结肠活检组织样本（n=63）,采用盲法,根据组织病理学评分和炎症程度分为五组,利用免疫组织化学法检测各组样本TLR4表达情况;利用FreeStyle 293表达系统制备TLR4-Ig融合蛋白并纯化。体外培养健康者来源的外周血单核细胞（PBMCs）,在培养基中添加100ug/ml LPS刺激或加入100ug/ml TLR4-Ig干预,检测10种炎性细胞因子分泌水平;动物实验方面,第一阶段设置五组：空白对照组、生理盐水组、IgG组、20mg/kg TLR4-Ig组、40mg/kg TLR4-Ig组;第二阶段设置六组：空白对照组、生理盐水组、IgG组、抗生素+IgG组、40mg/kg TLR4-Ig组、抗生素+40mg/kg TLR4-Ig组,抗生素处理组均于实验前4周至实验结束期间饮用“抗生素鸡尾酒”;除空白对照组外,各组小鼠均饮3%DSS溶液,两阶段实验均于第1、3、5、7天腹腔注射相关药物,每天记录小鼠体重变化、疾病活动指数（DAI）、生存率;分别于第9天和第7天处死所有小鼠,测量结肠长度,进行结肠的组织病理学评估。 结果 UC患者结肠组织TLR4表达水平与病变程度正相关;TLR4-Ig融合蛋白能够以高亲和力结合TLR4配体,阻断TLR4信号介导的PBMCs活化和炎性细胞因子的分泌;TLR4-Ig融合蛋白对TLR4信号的阻断加重了DSS诱导的小鼠急性结肠炎,给予小鼠抗生素处理对TLR4信号缺失造成的损伤加重有缓解作用。 结论 TLR4信号与肠道菌群的信号交流是UC发生早期的重要保护机制,TLR4信号的缺失不利于缓解急性炎症和修复肠黏膜。     

60Coγ射线辐射小鼠免疫功能改变及与肠道白色念珠菌定植关系的实验研究

目的探讨60Co辐照后免疫功能改变及其与白色念珠菌肠道定植的关系.方法预先口饲白色念珠菌悬液,3 d后行60Co一次全身照射(剂量分别为0、4.5及6.0Gy)后第9天采用MTT法测定小鼠腹腔中性粒细胞杀菌功能及脾T淋巴细胞的增殖活性的同时,定量研究小鼠肠道白色念珠菌的定植.结果辐射后第9天两辐照组(4.5、6.0Gy)的腹腔中性粒细胞杀菌率、脾T淋巴细胞增殖活性显著低于对照组(0Gy)(P＜0.01).而白色念珠菌的肠道定植量(对数值)显著高于对照组(P＜0.01).相关分析显示,中性粒细胞杀菌率及脾T淋巴细胞增殖活性均与白色念珠菌的肠道定植量呈负相关(P＜0.01).结论60Co γ射线一次全身辐照削弱了小鼠脾T淋巴细胞增殖活性,降低了腹腔中性粒细胞杀菌力,促进了白色念珠菌的肠道定植,为其内源性感染创造了条件.     

TLR4在益生菌治疗小鼠Hp感染中的作用

目的 比较BALB/c小鼠在幽门螺旋杆菌(Hp)感染后和用益生菌治疗后胃黏膜内Toll样受体4(TLR4)、MyD88和IL-1β在基因和蛋白水平上的表达变化,探讨Toll样受体4信号通路在益生菌治疗Hp感染中的作用.方法 60只感染Hp的BALB/c小鼠随机分为3组,第1组不予治疗,第2组予抗生素三联疗法治疗,第3组予乳酸杆菌治疗,另设20只正常小鼠为对照组.取各组小鼠的胃黏膜标本,用RT-PCR和Western blotting的方法 半定量检测TLR4、MyD88、IL-1β在基因和蛋白水平上的表达.结果 ①正常小鼠胃黏膜内无TLR4、MyD88和IL-1β表达.②益生菌治疗组小鼠的TLR4、MyD88和IL-1β均高于抗生素治疗组,但二者均低于Hp感染组.③各组小鼠TLR4与MyD88的表达均呈正相关.结论 TLR4信号通路参与了Hp的致病机制和益生菌治疗Hp的机制,其信号转导均可能是通过MyD88途径.     

TLR2 mAb和TLR4 mAb对急性期溃疡性结肠炎小鼠结肠黏膜炎症因子IFN-γ、IL-4及 IL-17表达的影响

 目的 探讨TLR2单克隆抗体（toll-like receptor 2 monoclonal antibodies,TLR2 mAb）和TLR4单克隆抗体（toll-like receptor 4 monoclonal antibodies,TLR4 mAb）对葡聚糖硫酸钠（dextran sulfate sodium,DSS）诱导的急性期溃疡性结肠炎（ulcerative colitis,UC）小鼠结肠黏膜的炎症因子IFN-γ、IL-4及IL-17表达的影响。方法 50只雄性BALB/c小鼠分为5组（每组10只）：对照组（A）、模型组（B）及TLR2 mAb干预组（C）、TLR4 mAb干预组（D）、TLR2 mAb和TLR4 mAb联合干预组（E）。A组小鼠饮用蒸馏水7 d;B-E组小鼠仅饮用5% DSS水溶液7 d以产生急性期溃疡性结肠炎模型。造模开始的同时,每隔48 h分别给予C、D、E干预组小鼠以TLR2 mAb（10 μg）、TLR4 mAb（10 μg）、TLR2 mAb（10 μg）+TLR4 mAb（10 μg）腹腔内注射,A、B组给予腹腔内注射生理盐水,以观察TLR2 mAb和TLR4 mAb的干预作用。观察指标包括疾病活动指数（disease activity index,DAI）、结肠组织病理学评分（histopathological score,HS）。造模及干预7 d后处死小鼠,Real-time PCR法检测各组结肠黏膜TLR2、TLR4、IFN-γ、IL-4及IL-17的mRNA表达。结果 (1)与A组相比,B组小鼠肠道DAI及HS明显增高（P<0.01）。与B组相比,E组小鼠肠道DAI（P<0.05）和HS（P<0.01）均明显降低。与D组相比,E组小鼠肠道HS明显降低（P<0.05）。(2)与A组相比,B组小鼠结肠黏膜TLR2、TLR4表达明显增高（P<0.01,P<0.05）。与B组相比,C、D、E组小鼠结肠黏膜TLR2、TLR4表达均有不同程度的降低。(3)与A组相比,B组小鼠结肠黏膜IFN-γ、IL-4及IL-17的mRNA表达明显增高（P<0.01,P<0.05,P<0.01）。与B组相比,C、D、E组小鼠结肠黏膜IFN-γ、IL-4及IL-17的mRNA表达均有不同程度的降低。结论 TLR2 mAb和TLR4 mAb可能通过下调小鼠结肠黏膜的炎症因子IFN-γ、IL-4、IL-17的mRNA表达而发挥其干预作用。     

TLR4mAb对急性期溃疡性结肠炎小鼠结肠黏膜中细胞因子IL－17、IL－10及TGF－β的影响

 目的 探讨TLR4单克隆抗体(toll like receptor 4 monoclonal antibodies，TLR4mAb) 对葡聚糖硫酸钠（dextran sulfate sodium,DSS）诱导的急性期溃疡性结肠炎（ulcerative colitis, UC）小鼠肠黏膜的细胞因子IL－17、IL－10及TGF－β的影响情况。方法 30只BALB/c小鼠分为A--E组：对照组、模型组以及低、中、高剂量干预组。A组小鼠饮用蒸馏水7d；B-E组小鼠仅饮用5%DSS水溶液共7d以产生急性期溃疡性结肠炎模型。造模开始的同时，分别给3组干预组小鼠以低、中、高剂量TLR4mAb腹腔内注射以观察其干预作用。观察指标包括疾病活动指数（disease activity index，DAI）、结肠组织病理学评分（histopathological score，HPS）。造模及干预7d后处死小鼠，Realtime-PCR法检测各组肠黏膜IL－17、IL－10及TGF－β的mRNA表达。结果（1）与对照组相比，模型组小鼠结肠黏膜DAI及HPS明显增高（P<0.01）。与模型组相比，在使用TLR4mAb干预后低、中、高剂量组DAI和HPS均有不同程度的缓解。（2）与模型组相比，在使用TLR4mAb后低、中、高剂量组细胞因子IL－17、IL－10及TGF－β在小鼠结肠黏膜中的表达均有不同程度的降低。结论TLR4mAb可以抑制肠道免疫的过度激活，减少炎症因子的过度表达，从而反馈性下调抑炎细胞因子的表达，打破促炎/抑炎因子的失衡状态，减轻急性期溃疡性结肠炎的炎症表现。     

旋毛虫对三硝基苯磺酸诱导肠炎小鼠Th1/Th2类细胞因子基因表达的影响

目的：研究旋毛虫对三硝基苯磺酸（TNBS）诱导的实验性小鼠肠炎模型Th1/Th2类细胞因子基因表达的影响。方法：雌性BALB/c小鼠随机分为50%乙醇对照组、旋毛虫（T. spiralis）应用组、 TNBS诱导肠炎模型组、预先感染T. spiralis后诱导TNBS模型组，（每组小鼠取材时保证6只以上）。采用RT-PCR方法观察感染旋毛虫和未感染旋毛虫小鼠于TNBS诱导肠炎后3 d和7 d时不同基因的表达变化，包括小鼠脾脏中IFN-γ、IL-4、IL-10 mRNA和结肠中IL-10 mRNA的表达量。结果：预先感染T. spiralis后诱导TNBS模型组小鼠在造模后3 d脾脏中IFN-γ mRNA的表达量与模型组比较差异无显著性(P>0.05)，在造模后7 d脾脏中IFN-γ mRNA的表达量低于模型组（P<0.05）， IL-4 mRNA的表达量于3 d和7 d时均高于模型组（P<0.05）。预先感染T. spiralis后诱导TNBS模型组小鼠在造模后3 d和7 d脾脏和结肠中IL-10 mRNA的表达量均高于模型组（P<0.05）。结论：旋毛虫对TNBS诱导的实验性小鼠肠炎外周免疫作用机制可能通过下调炎症性肠病过度的Th1型免疫反应、上调Th2型免疫反应而实现；IL-10既作为Th2类细胞因子又作为Tr1型细胞因子对实验性小鼠的肠道局部和外周免疫均产生重要调节作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.