中度失血性休克对大鼠重型脑损伤后S-100β表达的影响

目的：观察伴有休克大鼠重型颅脑损伤后颈静脉血清S-100β蛋白水平的变化。方法：雄性Wistar大鼠70只，采用Feeney法制作颅脑损伤模型，股动脉放血造成休克模型。随机分为7组。采用双抗体夹心酶联免疫分析单纯重型颅脑损伤组（SBI）及重型颅脑损伤伴休克组（SBIS）S-100β蛋白水平变化。结果：单纯颅脑损伤组及损伤加休克组均在伤后6h，S-100表达达到高峰，其余各时间点损伤加休克组均比单纯颅脑损伤组表达高，而且具有显著性差异（P〈0．01）。结论：失血性休克能引起重型颅脑损伤后严重继发性损伤，积极纠正休克对减轻脑伤后继发性病理损害意义重大。     

亚低温对重型脑损伤后血清IL-1β和S-100β蛋白含量的影响

目的观察重型创伤性脑损伤(severe traumatic brain injury,sTBI)患者血清IL-1β和S-100β蛋白含量变化以及亚低温治疗对IL-1β和S-100β蛋白含量的影响,探讨亚低温脑保护的可能机制。方法46例sTBI患者随机分成常温治疗(normothermia-treated,NT)组和亚低温治疗(mild hypothermia-treated,HT)组,分别予以常温治疗和亚低温治疗。两组均于伤后6, 24 h、3,8 d等各时间点采用酶联免疫吸附(ELISA)法测定IL-1β和S-100β蛋白含量;分析各时间点两组IL-1β和S-100β蛋白含量与GCS的相关性。结果(1)伤后各时间点两组sTBI患者血清IL-1β和S-100β蛋白含量明显高于对照组(P<0.01),但两组间IL-1β和S-100β蛋白含量在伤后6 h差异无统计学意义(P>0.05)。(2)亚低温治疗后各时间点HT组IL-1β和S -100β蛋白含量低于NT组(P<0.01)。(3)NT组于伤后各时间点IL-1β和S-100β蛋白含量均与GCS呈负相关;HT组IL-1β和S-100β蛋白含量于伤后24 h、3 d与GCS无相关性,6 h、8 d与GCS呈负相关;与NT组比较,出院时HT组预后较好。结论亚低温治疗能够降低sTBI患者IL-1β与S-100β蛋白含量,改善预后,具有脑保护作用。其脑保护机制可能与亚低温能减轻血清IL-1β和S-100β蛋白介导的损伤性脑细胞炎症反应有关。     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

目的 通过动态观察亚低温对急性重型颅脑损伤患者血清S-100B蛋白浓度的影响,探讨亚低温在急性重型颅脑损伤治疗中的作用.方法 将120例急性重型颅脑损伤患者随机数字表法分为亚低温组和常规组.亚低温组在常规治疗的基础上,予亚低温治疗,直肠温度维持在33～35℃,持续3～5 d.所有患者于入院6 h内,入院后第2,3,4,5,6天动态检测血清S-100B蛋白浓度.3个月后对患者进行GOS评估.结果 亚低温组和常规组血清S-100B蛋白浓度明显高于正常对照组(P<0.05);亚低温组血清S-100B蛋白浓度明显低于常规组(P<0.05);亚低温能够改善急性重型颅脑损伤患者的预后.结论 早期应用亚低温能显著降低急性重型颅脑损伤患者血清S-100B蛋白浓度,保护神经功能,改善预后,其脑保护作用可能与亚低温能减轻S-100B蛋白介导的损伤性脑细胞炎症反应有关.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

IL-1β及其受体在机械性脑损伤大鼠大脑皮质及海马中的表达

 目的通过复制Marmarou大鼠机械性脑损伤模型,研究脑损伤后白细胞介素1β(interlukin-1β,IL-1β)及其受体IL-1RI在大脑皮质和海马中表达的时序性特点及二者之间的相互关系.方法将60只清洁级雄性SD大鼠随机分为正常对照组、手术对照组及损伤后1h组、2 h组、4 h组、8 h组、12 h组、24h组、2 d组、3 d组、5 d组.采用组织芯片及免疫组化技术检测大脑皮质和海马中IL-1β、IL-1RI的表达情况.结果对照组大脑皮质、海马部位均表达少量IL-1β及IL-1RI.与对照组相比,损伤组皮质和海马中IL-1β的表达在损伤后30 min～12 h组显著升高(P＜0.01),并在2 h达高峰,然后逐渐降低,损伤后24 h降至正常水平,而皮质和海马中IL-1RI的表达在损伤后1～12 h组显著升高(P＜0.01),并在4 h达高峰,然后逐渐降低,损伤后72 h降至正常水平.结论IL-1β及IL-1RI的表达在闭合性脑损伤早期即显著升高,后期逐渐恢复正常,两者在损伤后表达的时序性变化特点基本一致,共同参与脑损伤的病理生理过程.     

S-100B蛋白评估重型脑损伤预后的临床研究

目的　研究重型脑损伤后血清S - 10 0B蛋白浓度变化 ,以期为重型脑损伤后原发性和继发性脑损害监测及预后评估提供直观的定量手段。　方法　对 2 0 0 2年 1月～ 2 0 0 2年 12月4 0例重型脑损伤住院患者伤后 12h～ 4d进行连续血清S - 10 0B蛋白浓度检测 ,并结合临床表现及格拉斯哥预后评分 (GOS)进行比较分析。　结果　本组 4 0例重型脑损伤患者伤后 12h血清S- 10 0B蛋白浓度均显著高于正常对照组 ,不同严重程度组之间S - 10 0B蛋白浓度差异存在非常显著性意义 (P <0 .0 1)。以伤后 12h血清S - 10 0B蛋白浓度 2 .0 0 μg/L为标准评估预后 ,其特异度为91% ,敏感度 72 % ,相对危险度 4 .33。本组 4 0例重型脑损伤患者伤后S - 10 0B蛋白浓度虽均呈下降趋势 ,但仍高于正常值 ,且预后恶劣组伤后各天S - 10 0B蛋白浓度均持续显著高于预后良好组 ,两组差异有非常显著性意义 (P <0 .0 1)。　结论　重型脑损伤后血清S - 10 0B蛋白浓度变化对原发性和继发性脑损害程度以及预后的评估有重要意义 ,为临床救治效果及病情转归的判断提供了有效手段。     

重型颅脑损伤伴颌面损伤失血性休克的早期救治

颅脑损伤伴颌面损伤的早期诊断和抢救较为困难,死亡率较高.作者治疗此类患者27例,抢救成功19例,成功率为70.4%.作者认为抢救此类患者应首先保持呼吸道通畅,积极止血、抗休克,再行头颅CT检查及手术.     

+GZ暴露对大鼠脑热休克蛋白-70表达水平的影响

目的 探讨＋Ｇｚ暴露条件下大鼠脑中热休克蛋白－７０（ＨＳＰ７０）的变化及其在＋Ｇｚ所致脑损伤中的作用。方法 １００只大鼠,随机分为４胡,每组２５只,一组作为空白对照,其余三组分别进行＋２Ｇｚ,＋６Ｇｚ和１０Ｇｚ暴露,峰值作用时间３ｍｉｎ,加速度增长率１Ｇ／ｓ然后,分别于暴露后的６ｈ、１ｄ、２ｄ、４ｄ和６ｄ麻醉大鼠,心脏灌注后迅速取脑组织,用Ｗｅｓｔ ｂｌｏｔ法比较ＨＳＰ７０的变化情况。结果 ＋Ｇｚ暴露后６ｈＨＳＰ７０开始升高,１ｄ达到高峰,至６ｄ时基本恢复正常。不同＋Ｇｚ暴露之间进行比较可以看出,＋６ＧＧｚ组ＨＳＰ７０表达最高,＋１０Ｇｚ最少。结论 ＋Ｇｚ暴露对大鼠脑ＨＳＰ７０表达水平有显著的影响。     

S-100B蛋白在颅脑损伤中的表达及其修复作用

介绍S-100B蛋白的生化性质及其在脑组织中特异性和高敏感性的表达。由于这些特点,S-100B蛋白作为损伤程度和预后的判断指标有重要的临床意义。另外,S-100B具有神经营养因子的作用,适量浓度的S-100B可在损伤部位产生神经再生效应。     

环孢素A抑制大鼠轴索损伤后血清IL-1β的表达

目的 观察大鼠轴索损伤后血清IL-1β的表达规律,以及环孢素A(CsA)对其表达的影响,探讨CsA的神经保护机制.方法 75只健康雄性SD大鼠随机分为3组:正常对照组(A组)5只、单纯视神经牵拉伤组(B组)35只、CsA处理组(C组)35只,B、C组又分为牵拉伤后或CsA治疗后1,3,6,12 h、1,3,7 d共7个观察时相点,不同时相点各5只大鼠.光镜下观察视网膜神经节细胞(RGCs)和轴索形态学变化,并采用放射免疫方法(平衡法)检测大鼠血清中IL-1β的含量.结果 (1)B组大鼠右侧视神经牵拉伤后3 d,RGCs明显稀疏;伤后7 d,神经纤维排列紊乱,分布稀疏.C组相应的病理变化明显减轻.(2)B组大鼠伤后3,6,12 h、1 d血清中IL-1β浓度明显高于A组,6 h达到高峰,随后逐渐下降,3 d后降至A组水平.C组大鼠血清IL-1β浓度变化规律与B组相似,3,6,12 h、l d血清中IL-1β浓度明显低于B组,但6,12 h、1 d仍高于A组.结论 IL-1β长时间过度表达可能参与了轴索损伤后的继发性病理变化;CsA可能通过减轻轴索损伤后的炎症反应对轴索损伤起保护作用.     

热休克蛋白与脑损伤的研究进展

热休克蛋白 (heatshockproteins ,HSPs)是指热应激时细胞新合成或合成增加的一类蛋白质 ,首次是从热应激果蝇幼虫的唾液腺等部位分离出来并命名。后来研究发现 ,除热应激外 ,多种损伤性因素可使组织细胞HSPs合成增加 ,它可保护机体在严酷的环境下得以生存。HSPs是一个大家族 ,现已发现的成员包括HSP1 1 0、HSP90家族、HSP70家族、HSP60、低分子量HSP、HSP1 0和泛素(ubiquitin)。正常生理情况下 ,中枢神经系统内并无HSPs的表达。近年来 ,国内外关于HSPs在脑损伤后的表达的研究十分活跃 ,已经发现缺血、创伤、氧化应激等均可导…