Local and systemic antibody response in Balb/c mice immunized with Entamoeba histolytica trophozoites.

Several immunization schedules with E. histolytica trophozoites were tested on Balb/c mice in order to induce antibody responses, both in intestinal secretions and in serum. Mice were immunized either orally, systemically, or using one of two combined schedules: the oral route followed by the systemic route (footpad), or vice versa. Each of the immunization schedules used in this project induced an anti-E. histolytica antibody response and there appears to be a correlation between the immunization route employed and the immunoglobulin isotype induced in the gut. Secretory IgA production is favored by the oral administration of trophozoites, whereas mucosal IgG appears to be enhanced by the systemic immunization route. Both schedules are effective in the induction of secretory IgA in the gut, yet higher and earlier levels of IgA appear in orally immunized mice. When systemic immunization is employed, the increase in antibody levels in the intestinal fluid is slower, and IgG is the predominant class. The combined oral/systemic routes of immunization appear to be comparably effective for the induction of local and systemic IgA and IgM antibody production. However, mice immunized first systemically and then locally produce more IgG in both compartments. Combined schedules modify the isotype pattern of antibody responses in serum and in intestinal secretions when compared with single (i.e., oral or systemic) schedules, but they do not appear to favor a secretory IgA immune response.     

HPV6b L1 VLPs经三种途径免疫小鼠的实验研究

目的：探讨加HPV6bL1VLPs经三种途径免疫小鼠后的免疫反应，以选择机体获得免疫保护的最佳途径。方法：小鼠以鼻腔吸入、阴道免疫、肌肉注射加HPV6bL1VLPs分为三组，经间隔2w三次免疫后取血、肺冲洗液和阴道冲洗液标本，用ELISA法检测血清IgG和分泌物IgA抗体，并用血凝抑制试验检测抗体的中和作用。结果：加HPV6bL1VLPs经肌肉注射、鼻腔吸入均能诱导产生血清蜘和呼吸道、阴道粘膜的IgA抗体，经阴道免疫可产生血清IgG和阴道粘膜的IgA抗体；抗体均具有中和活性；其中肌肉注射组产生的血清IgG抗体滴度最高，鼻腔免疫产生的粘膜I醇抗体滴度最高。结论：加HPV6bL1VLPs具较强抗原性；肌肉注射和鼻腔吸入免疫可刺激小鼠产生全身和局部粘膜的中和抗体，鼻腔吸入免疫可能是诱导机体产生粘膜免疫的最佳途径。     

慢性乙型肝炎患者血清免疫球蛋白水平的变化及临床意义

目的探讨慢性乙肝患者血清免疫球蛋白水平变化及其临床意义。方法采用免疫比浊法对我院2010年1月至2012年1月收治的100例乙型肝炎患者[其中慢性乙肝（CHB）患者53例、慢性重型乙肝（SHB）患者47例]及同期50例健康体检者（健康对照组）的血清免疫球蛋白（IgG、IgA和IgM）进行检测,比较各组患者免疫球蛋白水平。结果 CHB组与SHB组患者IgG、IgA和IgM水平均明显高于健康对照组,且SHB组免疫蛋白水平明显高于CHB组,差异均具有统计学意义（P〈0.05）;病情改善组IgG、IgA和IgM水平均明显低于恶化组,且两组均较治疗前显著变化,差异均具有统计学意义（P〈0.05）。结论血清免疫球蛋白水平能反映慢性乙肝患者肝细胞受损程度,可为临床诊断、治疗和预后提供检测依据。     

血清Ig、CRP联合肺功能检测在RMPP诊治中的应用

目的 探讨血清免疫球蛋白(Ig)、C反应蛋白(CRP)和肺功能检测在儿童难治性肺炎支原体肺炎(RMPP)诊治中的应用价值.方法 选取2015年1月至2016年6月3家医院收治的RMPP患儿82例为肺炎组,以基线资料匹配的同期体检健康儿童30例为对照组.比较两组血清IgA、IgG、IgM、IgE和CRP水平及同期1 s用力呼气容积(FEV1)、用力肺活量(FVC)等肺功能指标,分析其在RMPP诊断中的应用价值.结果 与对照组比较,肺炎组血清IgA、IgG水平、FEV1和FVC均降低,血清IgE和CRP水平均升高(P＜0.05).与治疗前比较,肺炎组治疗后的血清IgA、IgG水平、FEV1和FVC均升高,血清IgE和CRP水平均降低(P＜0.05).治疗前后肺炎组治疗无效患儿的血清IgA、IgG水平、FEV1和FVC均低于治疗有效患儿,血清IgE和CRP水平则高于治疗有效患儿(P＜0.05).RMPP患儿血清IgA、IgG、IgE、CRP水平与FEV1、FVC和治疗有效率均相关.结论 血清Ig、CRP水平及肺功能检测在儿童RMPP诊断中具有较大应用价值,值得在临床推广应用.     

免疫球蛋白亚类、IL-4和IL-6在恶性淋巴细胞血液病中的变化

目的　观察多发性骨髓瘤和淋巴细胞白血病患者血清中免疫球蛋白亚类、ＩＬ－４ 和ＩＬ－６ 的含量变化。方法　采用酶联免疫吸附法测定了５０ 例多发性骨髓瘤（ＭＭ）和淋巴细胞白血病患者血清ＩｇＧ亚类、ＩＬ－４ 和ＩＬ－６ 水平；用免疫单向琼脂扩散法测定了ＩｇＡ亚类水平；并对上述部分参数进行相关分析。结果　ＭＭ 患者中，ＩｇＧ型最多，副蛋白主要分布于ＩｇＧ１ 和ＩｇＧ２ ，其ＩｇＡ及亚类多缺乏；ＩｇＡ１ 型患者血清ＩｇＧ１ 明显降低。病程较长和复发的ＡＬＬ患者血清ＩｇＧ、ＩｇＡ和ＩｇＧ１ 水平均明显低于正常对照组（Ｐ＜０－０５）；初发ＡＬＬ患者接近正常水平。绝大多数ＡＬＬ患者ＩｇＡ１ 和ＩｇＡ２ 水平明显低于正常。同时发现ＡＬＬ患者血清ＩｇＧ１ 水平与ＩＬ－ ４ 含量呈正相关（ｒ＝０－６６８ ，Ｐ＜０－０５） 。结论　多发性骨髓瘤和淋巴细胞白血病患者容易合并免疫球蛋白亚类缺乏。     

恶性肿瘤患者输血前后免疫球蛋白测定结果分析

目的探讨输血对肿瘤患者血清免疫球蛋白IgG、IgA、IgM含量的影响作用。方法采用酶联免疫法测定恶性肿瘤患者、子宫肌瘤患者以及对照组患者输血前、后血清IgG、IgA、IgM的含量。结果恶性肿瘤患者血清免疫球蛋白含量低于正常，输血后5日，血清免疫球蛋白含量均见升高，IgG的含量变化最明显（P〈0.01），输血后2周，又回落到原有水平；子宫肌瘤组、对照组输血前、后5日及2周血清免疫球蛋白含量均无明显变化。三组患者输血前与输血后2周血清免疫球蛋白水平均无明显变化（P〉0.05）。结论恶性肿瘤患者输血后血清IgG、IgA、IgM含量发生改变是暂时的，为了最大限度地减少输血对恶性肿瘤患者免疫功能的影响，应开展成分输血的方法。     

儿童龋病唾液氧化应激和免疫指标与龋病严重程度的相关性

目的 探讨唾液氧化应激和免疫指标与儿童龋病严重程度的相关性.方法 选取2019年3月—2021年3月西安交通大学口腔医院儿童口腔科收治龋病患儿107例(龋病组),根据乳牙龋失补牙面数(dmft)分为低龋亚组(0～4,42例)、高龋亚组(≥5,65例),另选择83例健康儿童为健康对照组.所有受试者入组当日均采集唾液,检测...     

Serum immunoglobulin levels in normal children and adults in the Lebanese and other Arab Middle Eastern populations

Serum immunoglobulin levels were determined in 1000 serum samples of normal middle easterners. Ig levels in mothers at delivery were normal except for a depressed IgG level. In cord blood the level of IgG was higher than its level in mothers, but no IgA or IgD was found. IgD was formed in some instances in the sera of infants in the first week of life. IgG showed a pattern similar to that described by others with the lowest values at 1-2 months, increasing gradually so that by 1-3 years the normal adult blood level is reached. IgA levels continued to increase with age and although no definite explanation is available, it is interesting to speculate on the possible relationship to the increased incidence of intestinal parasitism. IgM showed significant differences between males and females being higher in the latter. IgD levels reached maximum levels by 3-5 years, and mean levels ere lower after the 14th year.     

贵州水、布依、苗族人群血清免疫球蛋白水平测定

对贵州省水、布依、苗三个少数民族健康人血清应用SDID法进行免疫球蛋白(Ig)水平调查。结果显示,布依族IgM女性明显高于男性,符合文献报道;三个民族的其余血清Ig水平男、女两性间的差别与文献报道相异;三个少数民族健康人血清Ig水平均值都高于国内健康汉族人的水平,为此应考虑到种族关系。     

Analysis of the relation between serum immunoglobulin and auto-antibody levels in patients with rheumatoid arthritis]

OBJECTIVE: To explore the relationship between serum immunoglobulin (Ig) levels and the positivity rate for auto- antibodies in patients with rheumatoid arthritis (RA). METHODS: The levels of immunoglobulins (IgG, IgM, IgA) and rheumatoid factor (RF) were measured using Olympus AU600 system, and indirect immunofluorescence and immunoblotting methods were employed respectively for detecting the autoantibodies in the serum from 40 RA patients and 30 normal adults. Statistical analysis of the relation between the Ig levels and autoantibodies was performed. RESULTS: Compared with normal control group, significantly higher serum IgA, IgG and IgM levels were detected in RA patients (P < 0.01), and at least one autoantibody was present in the serum sample from 26 out of the 40 RA patients, who had the highest RF positivity rate of 20% (8 cases). The serum IgA level in autoantibody-positive patients was significantly higher than that in autoantibody-negative ones (P < 0.01), and in the 18 patients with serum IgA level beyond the normal range, 15 (83.3%) were positive for autoantibody, while in the 22 patients with normal serum IgA level, the autoantibody positivity rate was only 50% (11/22), showing significant difference (P < 0.05). CONCLUSION: Serum immunoglobulins A (IgA) level is closely associated with the positivity rate for autoantibodies in RA patients, and RA patients with serum IgA level beyond the normal range was more likely to be positive for the autoantibodies.     

贵州汉、彝、回族人群血清免疫球蛋白水平测定

本文应用SDID法对贵州省汉、彝和回族健康人血清作了免疫球蛋白（Ig）水平调查。结果显示汉族三类Ig均低于彝、回族，而与国内健康汉族人无差异。     

类风湿性关节炎患者血清免疫球蛋白和自身抗体水平的检测与分析

目的测定类风湿性关节炎(RA)患者血清免疫球蛋白IgG、IgA、IgM和自身抗体(ANA、抗ds-DNA、抗ENA)水平,并探讨两者与RA及两者之间的关系。方法应用速率散射比浊法、间接免疫荧光法和免疫印迹法等对40例初诊RA患者和30例正常人血清IgG、IgM、IgA和类风湿因子(RF)及自身抗体水平进行检测。用t检验和字2检验分析这些自身抗体与Ig水平的关系。结果RA患者血清IgA、IgG、IgM水平升高,与正常人相比存在显著性差异(P<0.05)。40例患者中有26例检出1种或多种自身抗体或RF,其中以RF检出率最高,为8例。患者中自身抗体或RF阳性组IgA水平高于阴性组(P<0.01);IgA水平高于正常参考范围上限组自身抗体阳性率(83.3%,15/18)高于IgA水平在正常参考范围以内组(50%,11/22;P<0.05)。结论RA患者血清免疫球蛋白升高与自身抗体阳性关系密切,血清IgA水平升高者自身抗体阳性率明显高于IgA水平在参考范围以内者。     

健康妊娠妇女血清免疫球蛋白水平的变化及其参考区间的建立

目的:妊娠妇女处于一个特殊的生理时期,机体内的各项血液指标都会发生一定程度的改变,本研究旨在探讨健康妊娠妇女血清免疫球蛋白水平的变化,并建立其参考区间.方法:选择2019年8月至2019年10月在中南大学湘雅二医院产科进行孕期检查的369例健康妊娠妇女,按照孕周将其分为早孕组、中孕组和晚孕组,并选取123例健康非孕期妇...     

The response of ponies to Myxovirus influenzae A-equi 2. II. Immunoglobulin classes of antibody to the virus in serum and nasal secretions

Nasal secretions and serum were collected from ponies in order to determine the types of immunoglobulins responsible for antibody activity against Myxovirus influenzae A-equi 2. Using specific antisera to remove each immunoglobulin, the antiviral activity of serum was shown to be a property of IgG globulin, whereas in nasal secretions activity was found in both IgG and IgG(T) globulins. However in secretions the predominant activity was attributed to IgG(T) globulin.Because of its sensitivity to 2 mercaptoethanol, early serum antibody was assumed to belong to the IgM class of immunoglobulins.From its behaviour in sucrose density gradients and on Sephadex G 200 columns, the antibody in nasal secretions was assumed to be of 7S mobility not 11S such as is found in man.     

免疫球蛋白检验对乙型肝炎患者治疗的临床应用价值

目的 分析免疫球蛋白(Ig)检验对乙型肝炎患者治疗的临床应用价值。方法 选取2019年1月至2020年3月间南昌市洪都中医院收治的64例乙型肝炎患者作为研究对象,按病情严重程度将其分为轻型组(n=36)和重型组(n=28);另外选择同期在南昌市洪都中医院进行健康体检的20例健康者作为对照组,三组均给予免疫球蛋白检验,比较三组的血清IgA、Ig M、IgG水平。与此同时,跟踪64例乙型肝炎患者的具体治疗情况,根据病情改善分为改善组(n=41)和未改善组(n=23),再对比两组的血清IgA、IgM、IgG以及总胆红素水平。结果 经检验,重型组的血清IgA、IgM、IgG水平高于轻型组、对照组(P0.05);轻型组的血清IgA、IgM、IgG水平高于对照组(P0.05)。经跟踪、总结,64例乙型肝炎患者中有41例治疗后病情得到显著改善(改善组),23例治疗后未见明显有好转迹象(未改善组),改善组的血清IgA、Ig M、Ig G及总胆红素水平均低于未改善组(P0.05)。结论 通过血清IgA、IgM、IgG水平的检测,能有效判断乙型肝炎的病情严重程度,也能作为疾病诊断的重要标志物之一,再结合总胆红素水平,可判定疾病治疗效果,免疫球蛋白检验价值高。     

类风湿性关节炎患者补体、CRP及免疫球蛋白水平检测

目的检测并探讨类风湿性关节炎（RA）患者血清中补体（C3、C4）、C-反应蛋白（CRP）及免疫球蛋白水平（IgA、IgG、IgM）水平的变化及其对疾病的影响。方法采用速率散射比浊法检测60例RA患者与40例健康人血清C3、C4、CRP、IgA、IgG、IgM水平,比较30例RA患者治疗前后检测结果。结果RA患者血清中C3、CRP、IgA、IgG和IgM检测水平显著高于健康对照组（P〈0．01）,补体C4明显高于健康对照组（P〈0．05）,治疗后CRP检测水平显著低于治疗前（P〈0．01）,而治疗前后C3、C4、IgA、IgG、IgM无显著变化（P〉0．05）。结论补体、CRP、免疫球蛋白均参与类风湿性关节炎的发病,CRP还参与类风湿性关节炎的发展过程,观察补体、CRP和免疫球蛋白水平变化对疾病的诊断、指导治疗和评价预后有重要意义。     

恶性浆细胞病免疫蛋白特点研究

目的:了解恶性浆细胞病血清蛋白、免疫球蛋白(Ig)及血清蛋白电泳中M-蛋白的位置、浓度等特点.方法:(1)对不同类型的恶性浆细胞病(实验组)与相应Ig增高的非恶性浆细胞病(对照组)之间的三种Ig(IgG、IgA、IgM)进行比较;(2)对恶性浆细胞病之间的血清总蛋白(TP)、球蛋白(G)进行比较;(3)对恶性浆细胞病与非恶性浆细胞病之间TP、G进行比较;(4)分析不同恶性浆细胞病血清蛋白电泳中M-蛋白带的位置及浓度特点.结果:除恶性浆细胞病之间血清TP、G浓度无显著差异外,恶性浆细胞病与非恶性浆细胞病之间TP、G、不同类型恶性浆细胞病与相应Ig增高的非恶性浆细胞病之间的三种Ig(IgG、IgA、IgM)均有显著差异;恶性浆细胞病之间的电泳区带有各自的特点.结论:恶性浆细胞病Ig除相应单克隆蛋白显著升高外,其余两种Ig均显著减低;恶性浆细胞病血清TP、G浓度显著增高形成高球蛋白血症.     

Does anxiety reduce the secretion rate of secretory IgA in saliva?

The effects of anxiety, depression and psychological stress on the secretion rate of salivary immunoglobulin (Ig)A were examined in a cross-sectional study of 114 registered nurses. A single, timed (five minutes) sample of whole unstimulated saliva was collected from each nurse; at the time of collection, psychosocial data for each nurse were collected by questionnaire. Nurses who reported more frequent episodes of anxiety had significantly lower mean secretion rates of salivary IgA than did nurses who reported only occasional episodes of anxiety. The concentration of secretory IgA in saliva decreased as the salivary volume increased. It was not possible to demonstrate whether anxiety influenced IgA secretion in saliva independently of its effects on salivary flow.     

Serum immunoglobulins in newborn calves before and after colostrum feeding

Pre-colostral and post-colostral sera of seven Holstein calves and colostral whey were analyzed immunoelectrophoretically. IgM, IgG(1) (fast), and IgG(2) (slow) were demonstrated while IgA was not detected in serum of new-born calves before colostrum feeding. In post-colostral serum IgG, IgM, in relatively higher levels, and IgA were present which corresponded with the classes of immunoglobulins found in whey. These observations suggest that the developing bovine fetus may be capable of independent immune response.     

脐血IgG、IgA、IgM、C3、C4水平及其临床意义

目的检测新生儿脐血血清IgG、IgA、IgM、C3、C4的变化,探讨新生儿脐血的免疫功能。方法24例正常足月自然分娩新生儿脐血作为研究对象,26例健康成人献血员（男女不分）的血清作为对照。采用散射比浊法检测血清中IgG、IgA、IgM、C3、C4的水平,对结果进行统计学分析。结果脐血清中IgG、IgA的量与成人血清相比无显著性差异（P〉0.05）;IgM、C3、C4的量显著低于成人血清（P〈0.01）。结论脐血清中IgM、C3、C4的水平低于正常成人水平,IgG、IgA的水平与正常成人无显著差别。