癫痫患者脑脊液β_2-微球蛋白的改变

本文应用~(125)Ⅰ放射免疫法对72例癫痫(包括44例原发性癫痫和28例继发性癫痫)和21例坐骨神经痛患者脑脊液中β_2-微球蛋白含量进行了测定。结果显示:继发性癫痫患者脑脊液β_2-微球蛋白(1191.13±332.5μg/L,x±s)显著高于原发性癫痫(695.28±346.66μg/L,x±s)及对照组(776.75±388.59μg/L)(P<0.01),表明β_2-微球蛋白虽是非特异性诊断指标,但对鉴别继发性癫痫和原发性癫痫具有一定价值。     

男性膀胱癌患者血清性激素水平的检测

目的　探讨性激素在膀胱癌发病中的意义。方法 :采用放射免疫法检测 31例男性膀胱癌患者及 2 7例同年龄正常人对照组血清中的雌二醇 (EstradiolE2 )、孕酮 (ProgesteroneP)及睾酮 (TestosteroneT)水平。结果 :男性膀胱癌患者血清E2 、P、T分别为 44.98± 2 0 .96pg ml( x±s) ,0 .37± 0 .18ng ml( x±s) ,6 .2 5± 2 .37ng ml( x±s) ,而正常对照组E2 、P、T分别为 5 6 .0 1± 13.5 0pg ml( x±s) ,1.14± 0 .37ng ml( x±s) ,5 .15± 1.37ng ml( x±s) ,两者比较膀胱癌组T明显高于对照组 (P <0 .0 5 ) ;而E2 、P明显低于对照组 (P <0 .0 5 )。结论 :膀胱癌的发生可能与性激素代谢失衡有一定的关系     

白血病细胞钠,钾腺苷三磷酸酶活性的实验观察

应用“铷示踪法测定小鼠淋巴白血病细胞(P_3细胞)和人慢性粒细胞白血病细胞(K_(62)细胞)的钠钾转运活性,并同其各自相应正常组织细胞进行比较,发现此二种肿瘤细胞的钠,钾腺苷三磷酸酶(Na~+,K~+-ATP酶)转运活性(41.178±5.312和55.124±9.756nmol K~+/10~6 cells/h)远较正常细胞(5.615±2.879和15.911±3.913nmol K~+/10~6 cells/h)为高(P<0.001),而非Na~+,K~+-ATP酶转运活性(14.521±2.224和89.034±11.428nmol K~+/10~6 cells/h)与正常细胞(13.041±4.480和91.700±19.128nmol K~+/10~6 cells/h)无明显差异。证明此二种白血病细胞具有高Na~+,K~+-ATP酶活性的特征。     

老年人脑血管疾病急性期血浆TFPI含量变化及其意义

目的 :探讨出血和缺血性脑血管病患者血浆中组织因子途径抑制物 (TFPI)改变及其临床意义。方法 :对发病 1～ 3天的急性脑血管病患者 ,采用双夹心ELISA抗原测定法检测血浆中TFPI抗原含量。结果 :出血 (HCVD)组患者血浆TFPI含量为 146 0 8± 2 1 36 (117- 186ng/ml) x±s与对照组 144 80± 2 3 18ng/ml相比 ,无显著差异 (P >0 0 5) ;ICVD组 16 5 53± 6 7 50(81- 2 84ng/ml) x±s血浆TFPI含量明显高于对照组 ,差异显著 (P <0 0 1)。结论 :监测脑血管病病人的TFPI对指导临床治疗有一定意义。     

Adrenal gland

920640 Plasma catecholamines in thediagnosis of pheochromocytoma. JIN La(金律), et al. Cardiovasc Instit, CAMS, Beijing.Chin Cir J 1992; 7(3): 233-235. Mean plasma NE and E levels of 37 patientswith pheochro mocytoma were 6.087±5.048(x±s, range 0.250-27.898) and 0.725±1.748ng/ml (0.059-9.229),respectively. Plasma NE     

凝血因子检测对肝脏疾病的临床应用

[目的 ]探讨肝脏疾病对凝血机制和纤溶活化功能的影响 ,分析其与肝脏损害程度的关系。[方法 ]采用ACL - 2 0 0型美国库尔特全自动血凝仪分别检测肝炎 33例 ;肝硬化 4 0例 ;肝癌 2 8例 ;正常人 4 0例 ,血凝血酶原时间 (PT)、活化部分凝血活酶时间 (APTT)和纤维蛋白原 (FIB ) ;采用胶体金方法测定血浆D -二聚体。[结果 ]肝炎组的PT(1 3.6± 1 .0 6 )s ,APTT (32 .4± 4 .5 7)s ,FIB(2 .5 7± 0 .4 6 )g/L ,D -二聚体 (0 .89± 0 .2 2 )mg/L ;肝硬化组PT (1 7.7± 5 .6 0 )s ,APTT(4 3.4± 1 3.3)s,FIB (2 .1 0± 0 .88)g/L ,D -二聚体 (2 .4 6± 0 .6 1 )mg/L ;肝癌组PT(1 5 .0 5± 2 .74 )s,APTT (36 .8± 5 .5 5 )s ,FIB(3.5 0± 1 .1 6 )g/L ,D -二聚体 (2 .31± 0 .8)mg/L ;它们与正常组PT(1 3.0 2± 0 .6 2 )s ;(APTT32 .0± 2 .0 )s;FIB (2 .81± 0 .78)g/L ;D -二聚体 (0 .4 5± 0 .1 1 )mg/L比较有不同程度的差异。 [结论 ]不同病程肝脏疾病患者PT、APTT、FIB、D -二聚体均可异常 ,其中肝硬化患者的各项指标变化最为显著。     

人心房肌细胞特殊颗粒的电镜酶细胞化学和免疫电镜研究

本研究应用电镜CMP酶细胞化学和心钠素免疫电镜胶体金,分别标记人心房肌细胞中初级溶酶体和心钠素,进行鉴别定位观察和定量分析。心钠素颗粒数量居多,占78.7～79.3％,大小均一,直径0.20±0.05μm(x±s),分布于整个细胞质内,多成簇,尤以肌膜下,核极区多见。而溶酶体大小不一,直径0.30±0.19μm(x±s),显著大于心钠素颗粒(P<0.001),一般在核极区成簇分布,也见肌原纤维之间和肌膜下单个散在分布。     

中西医结合治疗异位妊娠28例临床观察

1998年 4月至 2 0 0 2年 6月 ,作者采用中西医结合治疗异位妊娠 2 8例 ,取得了满意疗效 ,现报道如下。1　临床资料1.1　一般资料　将住院确诊为未破裂型输卵管妊娠并符合药物治疗条件的 5 4例患者 ,分为治疗组 (2 8例 )和对照组 (2 6例 )。治疗组年龄 2 0～ 39岁 , x±s(2 8.4± 8.0 )岁 ;停经 35～6 8d , x±s(5 2 .1± 10 .1)d。对照组年龄 2 2～ 37岁 , x±s(2 9.1± 9.1)岁 ;停经 38～ 6 5d , x±s(4 9.8± 9.9)d。 2组年龄、停经时间经统计学处理差异无统计学意义。药物治疗条件 :①生命体征稳定 ,无明显腹腔内出血表现 ;②B超检查 ,…     

3.0 T磁共振弥散成像对肝脏恶性局灶性病变诊断的价值

对47例肝脏恶性局灶性病变进行磁共振弥散加权散成像，分析各种病变弥散成像的特点并测量肝脏和病变的表观弥散系数(ADC值)。结果：DWI图像显示，病灶呈较高信号46例，信号欠均匀以及边界欠清的病灶分别占51.06%(24/47)、44.68% (21/47)。当b值为400?s/mm2时，肝细胞癌、胆管细胞癌和转移瘤的ADC值分别为［(145.86±33.97)×10-3］?mm2/s、［(185±53.09)×10-3］?mm2/s 和［(212.62±81.72)×10-3］?mm2/s，转移瘤与肝脏的ADC值存在显著的差异(t=3.202, P=0.011)，肝细胞癌和胆管细胞癌与肝脏的ADC值之间无差异。转移瘤与肝细胞癌之间、肝细胞癌与胆管细胞癌之间的ADC值差异均具有统计学意义(P＜0.05)，转移瘤与胆管细胞癌的ADC值差异无统计学意义。结论：3.0 Tesla MR弥散成像有助于肝细胞癌、胆管细胞癌和转移瘤的诊断及鉴别     

应用微载体Cytodex 3高密度培养L-02人肝细胞系

目的　探讨用微载体进行 L - 0 2人肝细胞系高密度培养的方法 ,以期为生物人工肝提供有应用价值的生物材料。　方法　在限制贴壁的条件下 ,采用 Cytodex3为材料进行 L- 0 2人肝细胞的微载体培养 ,诱导肝细胞聚集体的形成 ,定期进行细胞计数及培养上清白蛋白、尿素、谷草转氨酶 (AST)、乳酸脱氢酶 (L DH )等生化指标的检测 ,并在光镜和透射电镜下观察生长情况。　结果　培养至第 6天时 ,所有的微载体均包满细胞 ,并形成微载体诱导的肝细胞聚集体 ,细胞数量达最高峰为 (17.1± 0 .76 )× 10 7/瓶 ,同时白蛋白和尿素的合成亦最高 ,浓度分别为5 3.81± 1.6 4m g/ L 和 5 .35± 0 .13m mol/ L。　结论　 Cytodex3培养的 L- 0 2人肝细胞可形成高密度的肝细胞聚集体 ,具有一定的生物合成和代谢功能。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.