Reversal of cisplatin-induced delay in gastric emptying in rats by ginger (Zingiber officinale)

Cisplatin causes nausea, vomiting and inhibition of gastric emptying. We have demonstrated the antiemetic effect of the acetone and ethanolic extract of ginger (Zingiber officinale, Roscoe, Zingiberacae) against cisplatin-induced emesis in dogs. In the present study, the acetone and 50% ethanolic extract of ginger in the doses of 100, 200 and 500 mg/kg (p.o.) and ginger juice, in the doses of 2 and 4 ml/kg, were investigated against cisplatin effect on gastric emptying in rats. All three ginger preparations significantly reversed cisplatin-induced delay in gastric emptying. The ginger juice and acetone extract were more effective than the 50% ethanolic extract. The reversal produced by the ginger acetone extract was similar to that caused by the 5-HT₃ receptor antagonist ondansetron; however, ginger juice produced better reversal than ondansetron. Therefore, ginger, an antiemetic for cancer chemotherapy, may also be useful in improving the gastrointestinal side effects of cancer chemotherapy.     

Ginger (Zingiber officinale) in migraine headache

Migraine is considered as a neurological disorder with little convincing evidence of the involvement of some vascular phenomenon. Recent understanding of the mechanisms behind migraine pain generation and perception have considerably helped the development of modern migraine drugs. Most migraine drugs in use, i.e., ergotamine and dihydroergotamine, iprazochrome, pizotifen and diazepam; and non-steroidal antiinflammatory drugs (i.e. aspirin, paracetamol, persantin, etc.) have side-effects and are prescribed with caution for a limited duration. Ginger is reported in Ayurvedic and Tibb systems of medicine to be useful in neurological disorders. It is proposed that administration of ginger may exert abortive and prophylactic effects in migraine headache without any side-effects.     

Zingiber officinale (ginger) compounds have tetracycline‐resistance modifying effects against clinical extensively drug‐resistant Acinetobacter baumannii

Extensively drug‐resistant Acinetobacter baumannii (XDRAB) is a growing and serious nosocomial infection worldwide, such that developing new agents against it is critical. The antimicrobial activities of the rhizomes from Zingiber officinale, known as ginger, have not been proven in clinical bacterial isolates with extensive drug‐resistance. This study aimed to investigate the effects of four known components of ginger, [6]‐dehydrogingerdione, [10]‐gingerol, [6]‐shogaol and [6]‐gingerol, against clinical XDRAB. All these compounds showed antibacterial effects against XDRAB. Combined with tetracycline, they showed good resistance modifying effects to modulate tetracycline resistance. Using the 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging method, these four ginger compounds demonstrated antioxidant properties, which were inhibited by MnO₂, an oxidant without antibacterial effects. After the antioxidant property was blocked, their antimicrobial effects were abolished significantly. These results indicate that ginger compounds have antioxidant effects that partially contribute to their antimicrobial activity and are candidates for use in the treatment of infections with XDRAB. Copyright © 2010 John Wiley & Sons, Ltd.     

A systematic review of the anti‐obesity and weight lowering effect of ginger (Zingiber officinale Roscoe) and its mechanisms of action

Recently, the beneficial effects of ginger on obesity is taken into consideration. Albeit, it seems that the anti‐obesity effect of ginger and its mechanism of action has not yet been reviewed. Therefore, the aim of this study was to systematically review the effect of Zingiber officinale Roscoe on obesity management. Databases including PubMed, Scopus, Google scholar, and Science Direct were searched from 1995 until May 2017 using the definitive keywords. Searching was limited to articles with English language. All of the relevant human and animal studies and also in vitro studies were included. Review articles, abstract in congress, and also other varieties of ginger were excluded. Eligibility of included articles were evaluated by 3 reviewers, which also extracted data. Articles were critically assessed individually for possible risk of bias. Twenty‐seven articles (6 in vitro, 17 animal, and 4 human studies) were reviewed. Most of the experimental studies supported the weight lowering effect of ginger extract or powder in obese animal models, whereas the results of the available limited clinical studies showed no changes or slight changes of anthropometric measurements and body composition in subjects with obesity. Ginger could modulate obesity through various potential mechanisms including increasing thermogenesis, increasing lipolysis, suppression of lipogenesis, inhibition of intestinal fat absorption, and controlling appetite. This review article provides some convincing evidence to support the efficacy of ginger in obesity management and demonstrates the importance of future clinical trials.     

Anxiolytic and antiemetic activity of Zingiber officinale

The benzene fraction (BF) of a petroleum ether extract of dried rhizomes of ginger, which contained anticonvulsant principle(s), was screened for anxiolytic and antiemetic activity. Motor coordination was not affected by BF per se, but diazepam-induced motor incoordination was potentiated. Animals treated with BF showed decreased occupancy in the closed arm of the elevated plus maze suggesting the presence of anxiolytic principles in the BF. BF also blocked lithium sulphate-induced conditioned place aversion indicating antiemetic activity. These findings suggest that the fraction (BF) possesses anticonvulsant, anxiolytic and antiemetic activity.     

Protective effects of Zingiber officinale (Zingiberaceae) against carbon tetrachloride and acetaminophen-induced hepatotoxicity in rats

The effect of the ethanol extract of the rhizome of Zingiber officinale was tested against carbon tetrachloride (CCl(4)) and acetaminophen-induced liver toxicities in rats. Increases in serum and liver marker enzymes such as alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, alkaline phosphatase as well as sorbitol and glutamate dehydrogenases were produced in normal rats that were not pretreated with the extract. However, extract-pretreated rats attenuated in a dose-dependent manner, CCl(4) and acetaminophen-induced increases in the activities of ALT, AST, ALP, LDH and SDH in the blood serum. The protective effect of the extract on CCl(4) and acetaminophen-induced damage was confirmed by histopathological examination of the liver. These results indicate that the oil from the rhizome of Zingiber officinale could be useful in preventing chemically induced acute liver injury.     

The effect of ginger (Zingiber officinale) on chemotherapy‐induced nausea and vomiting in breast cancer patients: A systematic literature review of randomized controlled trials

Breast cancer is the most commonly diagnosed cancer and is the leading cause of mortality due to all types of cancers among the female population worldwide. Results of clinical trials investigating the effect of ginger on chemotherapy‐induced nausea and vomiting (CINV) in breast cancer patients are inconsistent. This study was aimed at obtaining a comprehensive overview of the current evidence regarding the effectiveness of ginger as an antiemetic modality for controlling CINV in breast cancer patients. All published randomized controlled trials in English were systematically searched on Google Scholar, PubMed, Scopus, and Cochrane search databases up to June 2018. The outcome variable of interest was severity and the frequency of nausea and vomiting in patients. A total of 50 studies were found through search databases. After excluding duplicates, the 42 remaining studies were screened, and finally, nine trials were included, which were published between 2012 and 2017. Two studies have examined the effect of ginger on the frequency of nausea, five studies on the frequency of vomiting, seven studies on the severity of nausea, and three studies on severity of vomiting. A study evaluated the effectiveness of ginger in improving dietary intake in CINV. Our investigation suggests that ginger may reduce nausea in the acute phase of chemotherapy in patients with breast cancer. The effect of ginger on nausea and vomiting in other conditions requires more high‐quality clinical trials.     

Effects of combining extracts (from propolis or Zingiber officinale) with clarithromycin on Helicobacter pylori

Propolis and Zingiber officinale have been shown to be specifically targeted against Helicobacter pylori strains, to possess antiinflammatory, antioxidant and antitumoral activity and to be used in traditional medicine for the treatment of gastrointestinal ailments. Considering that these natural products could potentially serve as novel therapeutic tools also in combination with an antibiotic, the aim of this work was to evaluate their effect when combined with clarithromycin on clinical H. pylori isolates (n = 25), characterized in respect to both clarithromycin susceptibility and the presence of the cagA gene. The results showed that the combinations of propolis extract + clarithromycin and Z. officinale extract + clarithromycin exhibited improved inhibition of H. pylori with synergistic or additive activity. Interestingly, the susceptibility to combinations was significantly independent of the microbial clarithromycin susceptibility status. Only one H. pylori strain showed antagonism towards the Z. officinale extract + clarithromycin combination. The data demonstrate that combinations of propolis extract + clarithromycin and Z. officinale extract + clarithromycin have the potential to help control H. pylori-associated gastroduodenal disease.     

Ginger's (Zingiber officinale Roscoe) inhibition of rat colonic adenocarcinoma cells proliferation and angiogenesis in vitro

Ginger's (Zingiber officinale Roscoe) natural bioactives, specifically ginger extract and 6‐gingerol, were measured for their in vitro inhibition of two key aspects of colon cancer biology – cancer cell proliferation and angiogenic potential of endothelial cell tubule formation. Ginger extract was obtained via column distillation, while the 6‐gingerol was purchased from Calbiochem. Antiproliferation activity was assessed through tritiated thymidine ([³H]Tdr) incorporation studies of YYT colon cancer cells; the anti‐angiogenic ability of gingerol was assessed by a Matrigel assays using MS1 endothelial cells. These selected ginger bioactives had: 1) a direct effect on YYT rat cancer cell proliferation (6–1.5% ginger extract; 100–4 µM 6‐gingerol); 2) an indirect effect on MS1 endothelial cell function either at the level of endothelial cell proliferation or through inhibition of MS1 endothelial cell tube formation (100–0.8 µM). Compound 6‐gingerol was most effective at lower doses in inhibiting endothelial cell tube formation. These in vitro studies show that 6‐gingerol has two types of antitumor effects: 1) direct colon cancer cell growth suppression, and 2) inhibition of the blood supply of the tumor via angiogenesis. Further research is warranted to test 6‐gingerol in animal studies as a potential anticancer plant bioactive in the complementary treatment of cancer. Copyright © 2008 John Wiley & Sons, Ltd.     

Ethnopharmacologic investigation on tropane-containing drugs in Chinese solanaceous plants

The role of tropane-containing drugs derived from Solanaceae has long been recognized. This report deals with Chinese solanaceous plants, including historic, botanical, phytochemical, pharmacological and clinical aspects. Our investigation revealed that this kind of drug is constantly being confirmed to be of true therapeutic value; however, further studies are in order.     

Ginger (Zingiber officinale Roscoe) prevents the development of morphine analgesic tolerance and physical dependence in rats

Ethnopharmacological relevance

Ginger (Zingiber officinale Roscoe), a well-known spice plant, has been used traditionally in the treatment of a wide variety of ailments such as opiates withdrawal-induced disorders. However, its influences on opioid tolerance and dependence have not yet been clarified.

Materials and methods

Adult male Wistar rats were rendered tolerant to analgesic effect of morphine by injection of morphine (10 mg/kg, i.p.) twice daily for 8 days. To develop morphine dependence, rats given escalating doses of chronic morphine. To determine the effect of ginger on the development of morphine tolerance and dependence, different doses of ginger were administrated before morphine. The tail-flick and naloxone precipitation tests were used to assess the degree of tolerance and dependence, respectively.

Results

Our results showed that chronic morphine-injected rats displayed tolerance to the analgesic effect of morphine as well as morphine dependence. Ginger (50 and 100 mg/kg) completely prevented the development of morphine tolerance. In addition, concomitant treatment of morphine with 100 and 150 mg/kg attenuated almost all of the naloxone-induced withdrawal sings which include weight lose, abdominal contraction, diarrhea, petosis, teeth chattering, and jumping. In addition, morphine-induced L-type calcium channel over-expression in spinal cord was reversed by 100 mg/kg ginger.

Conclusion

The data indicate that ginger extract has a potential anti-tolerant/anti-dependence property against chronic usage of morphine.     

Analgesic, antiinflammatory and hypoglycaemic effects of ethanol extract of Zingiber officinale (Roscoe) rhizomes (Zingiberaceae) in mice and rats

The present study was undertaken to investigate the analgesic, antiinflammatory and hypoglycaemic effects of Zingiber officinale dried rhizomes ethanol extract (ZOE) in mice and rats. The analgesic effect of ZOE was evaluated by 'hot-plate' and 'acetic acid' analgesic test methods in mice; while the antiinflammatory and hypoglycaemic effects of the plant extract were investigated in rats, using fresh egg albumin-induced pedal oedema, and streptozotocin (STZ)-induced diabetes mellitus models. Morphine (MPN, 10 mg/kg), diclofenac (DIC, 100 mg/kg) and chlorpropamide (250 mg/kg) were used as reference drugs for comparison. ZOE (50-800 mg/kg i.p.) produced dose-dependent, significant (p < 0.05-0.001) analgesic effects against thermally and chemically induced nociceptive pain in mice. The plant extract (ZOE, 50-800 mg/kg p.o.) also significantly (p < 0.05-0.001) inhibited fresh egg albumin-induced acute inflammation, and caused dose-related, significant (p < 0.05-0.001) hypoglycaemia in normal (normoglycaemic) and diabetic rats. The findings of this experimental animal study indicate that Zingiber officinale rhizomes ethanol extract possesses analgesic, antiinflammatory and hypoglycaemic properties; and thus lend pharmacological support to folkloric, ethnomedical uses of ginger in the treatment and/or management of painful, arthritic inflammatory conditions, as well as in the management and/or control of type 2 diabetes mellitus in some rural Africa communities.     

Hypolipidaemic and Antiatherosclerotic Effects of Zingiber officinale in Cholesterol Fed Rabbits

Hyperlipidaemic rabbits when challenged with a 50% EtOH extract of Zingiber officinale showed a reduction in total cholesterol and serum LDL-cholesterol. A reduction in HDL ratio was seen in atherofed rabbits compared with controls which were restored when challenged with the Zingiber extract. An atherogenic index of 4.7 was brought down to 1.2 using plant products. The tissue lipid profiles of liver, heart and aorta showed similar changes to those noticed in serum lipids. Zingiber extract feeding increased the faecal excretion of cholesterol thus suggesting a modulation of absorption.     

生姜中二苯庚烷类化合物的抗氧化 和细胞毒活性研究

目的：研究生姜中二苯庚烷类化合物(1 ~ 5)的抗氧化和细胞毒活性。方法：采用吸光度测试法检测化合物于50 mg &#8226;L–1清除超氧阴离子的效果,同法检测化合物(1 ~ 5)清除1,1-二苯基苦基苯肼(DPPH)自由基之半数清除浓度（IC50）,用MDA检测法检查化合物于100 mg&#8226;L–1浓度下抑制大鼠脑匀浆脂质过氧化的能力,以及用MTT法研究化合物抑制过氧化氢(H2O2)诱导的大鼠肾上腺嗜铬瘤细胞(PC12)损伤的活性,并采用MTT法检测化合物对人慢性髓源性白血病细胞株(K562)及其阿霉素耐药株(K562/ADR)的细胞毒活性（IC50）。 结果：化合物1 ~ 5对DPPH自由基有较好的清除作用,化合物5的清除作用最强,IC50（22.6 ± 2.4）μmol&#8226;L-1;化合物1,3和4有抑制大鼠脑匀浆脂质过氧化作用,100 mg&#8226;L–1其抑制率分别为(66.3 ± 15.4)%,(68.7 ± 15.8) %,(72.2 ± 10.6)%;化合物1,3和4对H2O2诱导的PC12细胞损伤有明显的保护作用,且呈量效关系;化合物3对K562及其阿霉素耐药株(K562/ADR)体外增殖有抑制作用,其IC50分别为(34.9 ± 0.6),（50.6 ± 23.5） μmol&#8226;L-1。结论：生姜中的二苯庚烷类化合物1 ~ 5具有特异性的抗氧化作用,化合物3对K562和K562/ADR细胞均显示出显著的细胞毒作用。     

生姜的药理研究和临床运用

<正> 生姜Zingiber officinale(willd.)Rosc.为常用中药,具有发汗解表,温中止呕,温肺止咳,解毒等功效。可用于外感风寒、咳嗽、肺痿、吐利、胸痹和痞证等,民间多用其解药物及食物中毒。     

Mosquito larvicidal activity of isolated compounds from the rhizome of Zingiber officinale

The larvicidal activity of a petroleum ether extract of Zingiber officinale Roscoe (Zingiberaceae) was evaluated against Aedes aegypti L. and Culex quinquefasciatus Say (Diptera). Bioassay-guided fractionation led to the isolation of 4-gingerol (1), (6)-dehydrogingerdione (2) and (6)-dihydrogingerdione (3); the latter has not previously been reported from Z. officinale. The structures were established from infrared (IR), ultraviolet (UV), (1)H-nuclear magnetic resonance (NMR), (13)C-NMR and mass spectral data. Following a 24 h exposure, compounds 1-3 exhibited larvicidal activities against fourth instar larvae of A. aegypti (LC(50) 4.25, 9.80, 18.20 ppm) and C. quinquefasciatus (LC50 5.52, 7.66, 27.24 ppm), respectively. The results show that the most effective compound was 4-gingerol.     

Protective effects of dietary ginger (Zingiber officinales Rosc.) on lindane-induced oxidative stress in rats

The protective effect of dietary feeding of Zingiber officinales Rosc. (ginger) against lindane-induced oxidative stress was investigated in male albino rats. Oxidative stress was monitored by estimating the extent of lipid peroxidation, activities of the oxygen free radical (OFR) scavenging enzymes superoxide dismutase (SOD) and catalase (CAT) and the status of the glutathione redox cycle antioxidants. Lindane administration (30 mg/kg bw orally for 4 weeks) was associated with enhanced lipid peroxidation and compromised antioxidant defenses in rats fed a normal diet. Concomitant dietary feeding of ginger (1%w/w) significantly attenuated lindane-induced lipid peroxidation, accompanied by modulation of OFR scavenging enzymes as well as reduced glutathione (GSH) and the GSH dependent enzymes glutathione peroxidase (Gpx), glutathione reductase (GR) and glutathione-S-transferase (GST) in these rats. These findings suggest that a diet containing naturally occurring compounds is effective in exerting protective effects by modulating oxidative stress.     

Effect of ethanolic extract of Zingiber officinale on dyslipidaemia in diabetic rats

The lipid lowering and antioxidant potential of ethanolic extract of Zingiber officinale Roscoe (family, Zingiberaceae) was evaluated in streptozotocin (STZ)-induced diabetes in rats. Ethanolic extract of Zingiber officinale (200 mg/kg) fed orally for 20 days produced, significant antihyperglycaemic effect (P < 0.01) in diabetic rats. Further, the extract treatment also lowered serum total cholesterol, triglycerides and increased the HDL-cholesterol levels when compared with pathogenic diabetic rats (P < 0.01). STZ-treatment also induced a statistically significant increase in liver and pancreas lipid peroxide levels (P < 0.01) as compared to normal healthy control rats. Zingiber officinale extract treatment lowered the liver and pancreas thiobarbituric acid reactive substances (TBARS) values (P < 0.01) as compared to pathogenic diabetic rats. The results of test drug were comparable to gliclazide (25 mg/kg, orally), a standard antihyperglycaemic agent. The results indicate that ethanolic extract of Zingiber officinale Roscoe can protect the tissues from lipid peroxidation. The extract also exhibit significant lipid lowering activity in diabetic rats. The present study is the first pilot study to assess the potential of Zingiber officinale in diabetic dyslipidaemia.     

Stability examination of beta-cyclodextrin inclusion compound of Zingiber officinale volatile oil]

Based on different storage time and different methods, examination has been conducted of the appearance, properties contents and main constituents in the beta-cyclodextrin inclusion compound of Zingiber officinale volatile oil. The result shows that the oil with inclusion proves considerably more stable than that without inclusion.     

Gastroprotective activity of ginger zingiber officinale rosc., in albino rats

The cytoprotective and gastric anti-ulcer studies of ginger have been carried out in albino rats. Cytodestruction was produced by 80% ethanol, 0.6M HC1, 0.2M NaOH and 25% NaCl. Whereas gastric ulcers were produced by ulcerogenic agents including indomethacin, aspirin and reserpine, beside hypothermic restraint stress and by pylorus ligated Shay rat technique. The results of this study demonstrate that the extract in the dose of 500 mg/kg orally exert highly significant cytoprotection against 80% ethanol, 0.6M HC1, 0.2M NaOH and 25% NaCl induced gastric lesions. The extract also prevented the occurrence of gastric ulcers induced by non-steroidal anti-inflammatory drugs (NSAIDs) and hypothermic restraint stress. These observations suggest cytoprotective and anti-ulcerogenic effect of the ginger.