ANTITUMOR EFFECT OF INTRATUMORAL INJECTION OF LIPOSOME-ENCAPSULATED G-CSF GENE AND IN SITU BIOLOGICAL CHARACTERISTICS OF THE TREATED TUMOR CELLS

ＡＮＴＩＴＵＭＯＲＥＦＦＥＣＴＯＦＩＮＴＲＡＴＵＭＯＲＡＬＩＮＪＥＣＴＩＯＮＯＦＬＩＰＯＳＯＭＥＥＮＣＡＰＳＵＬＡＴＥＤＧＣＳＦＧＥＮＥＡＮＤＩＮＳＩＴＵＢＩＯＬＯＧＩＣＡＬＣＨＡＲＡＣＴＥＲＩＳＴＩＣＳＯＦＴＨＥＴＲＥＡＴＥＤＴＵＭＯＲＣＥＬＬ...     

THE EFFECT OF ACTIVE COMPONENTS OF LYCIUM BARBARUM AND GARLIC（LB－GO）ON THE SYNTHESIS OF DNA AND ULTRASTRUCTURE OF U_（14） CERV

ＴＨＥＥＦＦＥＣＴＯＦＡＣＴＩＶＥＣＯＭＰＯＮＥＮＴＳＯＦＬＹＣＩＵＭＢＡＲＢＡＲＵＭＡＮＤＧＡＲＬＩＣ（ＬＢ－ＧＯ）ＯＮＴＨＥＳＹＮＴＨＥＳＩＳＯＦＤＮＡＡＮＤＵＬＴＲＡＳＴＲＵＣＴＵＲＥＯＦＵ_（１４）ＣＥＲＶＩＸＣＡＮＣＥＲＣＥＬＬＳＩＮＭＩＣ?..     

THE BLOCKING EFFECTS OF GLYCYRRHIZE URALENSIS AND CHELIDONIUM MAJUS ON MUTAGENESIS INDUCED BY AFLATOXIN B1

ＴＨＥＢＬＯＣＫＩＮＧＥＦＦＥＣＴＳＯＦＧＬＹＣＹＲＲＨＩＺＥＵＲＡＬＥＮＳＩＳＡＮＤＣＨＥＬＩＤＯＮＩＵＭＭＡＪＵＳＯＮＭＵＴＡＧＥＮＥＳＩＳＩＮＤＵＣＥＤＢＹＡＦＬＡＴＯＸＩＮＢ１ＳｈｉＧｕｉｚｎｉ；史桂芝；ＪｉＸｉｎｈｕａ；纪新华；ＬｉａｎｇＹ...     

THE THERAPEUTIC EFFECT OF INTRATUMORAL INJECTION OF GM-CSF GENE-MODIFIED ALLOGENIC MACROPHAGES ON TUMOR-BEARING MICE

ＴＨＥＴＨＥＲＡＰＥＵＴＩＣＥＦＦＥＣＴＯＦＩＮＴＲＡＴＵＭＯＲＡＬＩＮＪＥＣＴＩＯＮＯＦＧＭＣＳＦＧＥＮＥＭＯＤＩＦＩＥＤＡＬＬＯＧＥＮＩＣＭＡＣＲＯＰＨＡＧＥＳＯＮＴＵＭＯＲＢＥＡＲＩＮＧＭＩＣＥＹｕＹｉｚｈｉ于益芝ＣａｏＸｕｅｔａｏ?..     

THE ANTITUMOR ACTIVITIES OF GNIDIMACRIN ISOLATED FROM STELLERA CHAMAEJASMEL．

ＴＨＥＡＮＴＩＴＵＭＯＲＡＣＴＩＶＩＴＩＥＳＯＦＧＮＩＤＩＭＡＣＲＩＮＩＳＯＬＡＴＥＤＦＲＯＭＳＴＥＬＬＥＲＡＣＨＡＭＡＥＪＡＳＭＥＬ．ＦｅｎｇＷｅｉｊｉａｎ；冯威健ＩｋｅｋａｗａＴｅｔｓｕｒｏ；ＹｏｓｈｉｄａＭｉｔｓｕｚｉ（ＦｏｕｒｔｈＨｏｓｐｉｔ...     

PROMOTION OF CHEMICAL CARCINOGENESIS AND P53 EXPRESSION BY REDUCTION OF SUPEROXIDE DISMUTASE ACTIVITY IN THE LUNG OF RAT IN V

ＰＲＯＭＯＴＩＯＮＯＦＣＨＥＭＩＣＡＬＣＡＲＣＩＮＯＧＥＮＥＳＩＳＡＮＤＰ５３ＥＸＰＲＥＳＳＩＯＮＢＹＲＥＤＵＣＴＩＯＮＯＦＳＵＰＥＲＯＸＩＤＥＤＩＳＭＵＴＡＳＥＡＣＴＩＶＩＴＹＩＮＴＨＥＬＵＮＧＯＦＲＡＴＩＮＶＩＶＯ￥ＹｕＬｕｎｙｉｎ；喻伦银；Ｂｉ...     

Basic Investigations EXPRESSION OF GAP JUNCTION PROTEIN Cx43 IN CULTURED HUMAN NORMAL AND MALIGNANT LUNG CELLS

ＢａｓｉｃＩｎｖｅｓｔｉｇａｔｉｏｎｓＥＸＰＲＥＳＳＩＯＮＯＦＧＡＰＪＵＮＣＴＩＯＮＰＲＯＴＥＩＮＣｘ４３ＩＮＣＵＬＴＵＲＥＤＨＵＭＡＮＮＯＲＭＡＬＡＮＤＭＡＬＩＧＮＡＮＴＬＵＮＧＣＥＬＬＳＺｈａｎｇＺｈｉｑｉａｎ；张志谦；ＬｉｎＺｈｏｎｇｘｉａｎｇ...     

THE EXPRESSION OF CYTOKERATINS IN HUMAN HEPATOCELLULAR AND CHOLANGIOCELLULAR CARCINOMAS

ＴＨＥＥＸＰＲＥＳＳＩＯＮＯＦＣＹＴＯＫＥＲＡＴＩＮＳＩＮＨＵＭＡＮＨＥＰＡＴＯＣＥＬＬＵＬＡＲＡＮＤＣＨＯＬＡＮＧＩＯＣＥＬＬＵＬＡＲＣＡＲＣＩＮＯＭＡＳ￥ＳｕＱｉｎ；苏勤；Ｌｉｕｙａｎｆａｎｇ；刘彦仿（ＤｅｐａｒｔｍｅｎｔｏｆＰａｔｈｏｌｏｇｙ，...     

IMMUNOLOGICAL CHARACTERISTICS OF THE LEUKEMIA CELLS TRANSFECTED WITH ONCOSTATIN M GENE BY RECOMBINANT ADENOVIRUS

ＩＭＭＵＮＯＬＯＧＩＣＡＬＣＨＡＲＡＣＴＥＲＩＳＴＩＣＳＯＦＴＨＥＬＥＵＫＥＭＩＡＣＥＬＬＳＴＲＡＮＳＦＥＣＴＥＤＷＩＴＨＯＮＣＯＳＴＡＴＩＮＭＧＥＮＥＢＹＲＥＣＯＭＢＩＮＡＮＴＡＤＥＮＯＶＩＲＵＳ１ＷａｎｇＱｕａｎｘｉｎｇ王全兴ＣａｏＸｕｅｔａｏ２...     

THE ROLE OF TARGETING THERAPY IN CYTOREDUCTION AND SEQUENTIAL RESECTION OF UNRESECTABLE HEPATOCELLULAR CARCINOMA

ＴＨＥＲＯＬＥＯＦＴＡＲＧＥＴＩＮＧＴＨＥＲＡＰＹＩＮＣＹＴＯＲＥＤＵＣＴＩＯＮＡＮＤＳＥＱＵＥＮＴＩＡＬＲＥＳＥＣＴＩＯＮＯＦＵＮＲＥＳＥＣＴＡＢＬＥＨＥＰＡＴＯＣＥＬＬＵＬＡＲＣＡＲＣＩＮＯＭＡＴａｎｇＺｈａｏｙｏｕ汤钊猷ＹｕＹｅｑｉｎ余业勤Ｚｈ...     

姜黄素诱导低分化鼻咽癌细胞株CNE-2Z的凋亡

背景与目的:鼻咽癌(nasopharyngealcarcinoma,NPC)是我国南方地区的高发肿瘤,发病早期的治疗主要是以放疗为主,而对晚期NPC患者则有必要进行适当的化疗。一些药物可通过诱导肿瘤细胞凋亡来达到治疗肿瘤的目的。本研究拟探讨姜黄素对人鼻咽癌细胞株CNE-2Z体外增殖抑制作用及凋亡的影响。方法:不同浓度姜黄素处理CNE-2Z细胞,用噻唑蓝(MTT)法测定增殖抑制率和IC50;用流式细胞术、Hoechest33258/碘化丙啶(PI)双染、琼脂糖电泳法观察细胞凋亡。结果:姜黄素能抑制CNE-2Z细胞的生长,其效果与姜黄素的浓度和作用时间有关,作用24h、48h、72h的IC50值为(24.05±0.47)、(19.20±0.17)、(7.35±0.50)μmol/L;5、10、20μmol/L姜黄素处理细胞24h后,流式细胞术观察到细胞凋亡率分别为(4.9±3.2)%、(10.7±2.7)%和(14.7±0.5)%;10、20μmol/L姜黄素处理细胞24h后,荧光染色可见细胞缩小,染色质固缩,核染色体碎裂等凋亡形态学改变,琼脂糖凝胶电泳可见DNA梯形条带。结论:姜黄素可诱导CNE-2Z细胞凋亡,姜黄素对CNE-2Z细胞具有增殖抑制作用。     

整合素αV对鼻咽癌CNE-2Z细胞放疗敏感性的影响

目的：探讨粘附分子整合素αV通过细胞间相互作用，是否对鼻咽癌CNE-2Z细胞的放疗敏感性产生影响，进而初步探讨其可能机制。方法：采用liquid overlay技术进行CNE-2Z细胞三维立体（多细胞球）培养；血球计数器计数法比较在阻断整合素αV作用前后CNE-2Z细胞的放射敏感性；AnnexinV／PI双标流式细胞术检测细胞凋亡。结果：（1）成功建立CNE-2Z多细胞球培养模型；（2）CNE-2Z多细胞球在接受X射线照射后，并在一定的照射剂量下，其表达的整合素αV量，随着照射剂量的增加而增加；（3）阻断整合素αV作用后CNE-2Z多细胞球放疗敏感性增强、细胞凋亡率上调。结论：以多细胞球培养模型模拟体内实体瘤情况，证实粘附分子整合素αV的表达情况可以影响鼻咽癌CNE-2Z细胞的放疗敏感性，抑制细胞凋亡是其可能的作用机制。     

脂质体介导bcl-xL反义寡核苷酸对鼻咽癌CNE-2Z细胞株凋亡的影响

背景与目的:实验证明 bcl-xL在鼻咽癌 CNE-2Z细胞中存在高表达 , 它可能在鼻咽癌的发生发展中发挥着重要的作用 . 本研究以脂质体 (lipofectin)为载体 , 将人工合成的 bcl-xL反义寡核苷酸 (antisense oligodeoxynucleotide,ASODN)片段导入 CNE-2Z细胞 , 拟探讨 ASODN对 CNE-2Z细胞的影响 . 方法:以 bcl-xL的编码区为靶点 , 人工合成 20个碱基全硫代修饰的反义寡核苷酸 , 脂质体转染反义寡核苷酸进入 CNE-2Z细胞后 , 用 MTT法检测细胞成活率 ; 用琼脂糖凝胶电泳、荧光染色、流式细胞术等方法检测细胞凋亡 . 结果:MTT结果发现 , ASODN在 Lip介导下即可显著抑制 CNE-2Z细胞株细胞增殖 (P< 0.01), ASODN对细胞增殖的抑制作用随其浓度增加而增强 ; ASODN作用细胞 36 h后 , 经 Hoechst 33258/PI双染在荧光显微镜下可见细胞缩小、染色质固缩、核断裂 ; 流式细胞仪分析发现在 G1峰前出现一个亚二倍体峰即凋亡峰 ; 琼脂糖凝胶电泳分析可见 ASODN/Lip处理组出现明显的 " 梯状 " 外观等凋亡特征性改变 . 结论:ASODN脂质体转染 CNE-2Z细胞后可促进 CNE-2Z的细胞凋亡 ; 在肿瘤研究中 , bcl-xL可作为一个有用的靶基因 , 为开发鼻咽癌基因治疗药物提供实验依据 .     

整合素αⅤ对鼻咽癌CNE-2Z细胞放疗敏感性的影响

目的:探讨粘附分子整合素αV通过细胞间相互作用,是否对鼻咽癌CNE-2Z细胞的放疗敏感性产生影响,进而初步探讨其可能机制。方法:采用liquid overlay技术进行CNE-2Z细胞三维立体(多细胞球)培养;血球计数器计数法比较在阻断整合素αV作用前后CNE-2Z细胞的放射敏感性;AnnexinV/PI双标流式细胞术检测细胞凋亡。结果:(1)成功建立CNE-2Z多细胞球培养模型;(2)CNE-2Z多细胞球在接受X射线照射后,并在一定的照射剂量下,其表达的整合素αV量,随着照射剂量的增加而增加;(3)阻断整合素αV作用后CNE-2Z多细胞球放疗敏感性增强、细胞凋亡率上调。结论:以多细胞球培养模型模拟体内实体瘤情况,证实粘附分子整合素αV的表达情况可以影响鼻咽癌CNE-2Z细胞的放疗敏感性,抑制细胞凋亡是其可能的作用机制。     

整合素α Ⅴ对鼻咽癌CNE-2Z细胞放疗敏感性的影响

Objective:To investigate whether the cell adhesion molecule integrin aV could influence the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cells via effecting intercellular effect.Methods:Human NPC cell line CNE-2Z was cultivated as three dimensional modef using liquid overlay technique.Cell counting was employed to detect the radiosensitivity of CNE-2Z cells by blood cell counter before and after blocking integrin aV function;cell apoptosis was detected by flow cytometry using Annexin V/PI label.Results:Three dimensional culture model of human NPC cell line CNE-2Z cells were successfully established The integrin aV expression of CNE-2Z multicellular spheroids elevated as the dosage of radiotherapy increased.Blocking of integrin aV function leaded to higher radiosensitivity and more apoptotic cells of CNE-2Z multicellular spheroids.Conclusion:Cell adhesion molecules integrin aV can influence the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cells and the inhibition of cell apoptosis might be its mechanism.proved by three dimensional culture model to mimic solid tumor in vivo.     

uPAR在鼻咽癌细胞系及其克隆株中的表达和作用

目的　探讨尿激酶型纤溶酶原激活剂受体 (uPAR )在鼻咽癌细胞系 (CNE 2Z)及其克隆株 (CNE 2Z H 5 ,CNE 2Z H 5 9)中的表达和作用。方法　采用逆转录聚合酶链反应 (RT PCR )方法 ,检测uPAR在基因转录水平的表达 ;采用侵袭抑制实验 ,观察uPAR在鼻咽癌细胞侵袭和转移过程中的作用。结果　uPAR在CNE 2Z H 5 9中表达最高 ,在CNE 2Z中表达最低 ,在CNE 2Z H 5中表达处于两者之间 ;抗uPAR抗体抑制CNE 2Z H 5 9的侵袭能力。结论　uPAR可能促进鼻咽癌细胞的侵袭和转移     

Effects of RNA interference targeting four different genes on the growth and proliferation of nasopharyngeal carcinoma CNE-2Z cells

To explore the effects of RNA interference targeting four different genes (VEGF, C-myc, Survivin, hTERT) on the growth and proliferation of nasopharyngeal carcinoma (NPC) CNE-2Z cells. Fluorescein-labeled short-hairpin (sh)RNA plasmids together and separately targeting VEGF, C-myc, Survivin and hTERT were built and respectively called plasmid-shVEGF-shC-myc-shSurvivin-shhTERT, plasmid-shVEGF, plasmid-shC-myc, plasmid-shSurvivin, plasmid-shhTERT. These plasmids were respectively transfected into human NPC CNE-2Z cells and xenograft tumors in nude mice. The expression of plasmids in NPC CNE-2Z cells and xenograft tumors was observed. Cell proliferation was detected with MTT assay. The mRNA and protein expression were determined by real-time PCR and western blot, respectively. The effects of plasmids on the biological behavior of CNE-2Z cells were observed with transwell invision chamber models. Apoptosis was determined with flow cytometer. The inhibitory effect of plasmids on xenograft tumors was observed in nude mice. The plasmid containing four different shRNAs could significantly inhibit CNE-2Z cell proliferation and decrease invasion ability in vitro compared with plasmids with each single shRNA (P<0.05). The plasmid containing four different shRNAs could simultaneously downregulate VEGF, C-myc, surviving, hTERT mRNA and protein expression in the CNE-2Z cells. The multiple gene shRNA could more significantly induce cell apoptosis than each single shRNA, respectively (P<0.05). The combinative silencing of these four genes had a better inhibitory effect on xenograft tumors than the silencing of each single shRNA (P<0.05). RNA interference targeting multiple genes can effectively inhibit NPC proliferation and induce apoptosis, which provides an experiment basis for NPC gene therapy.     

PNCK对鼻咽癌细胞增殖和凋亡作用的研究

目的 研究妊娠上调的非泛素性钙调蛋白激酶(Pregnancy-upregulated nonubiquitous calmodulin kinase,PNCK)在鼻咽癌组织中的表达以及对鼻咽癌细胞增殖和凋亡的作用。方法 采用免疫组织化学方法检测鼻咽癌癌组织和正常组织中PNCK表达水平。构建慢病毒载体干扰PNCK基因表达,并采用Real time PCR和免疫印迹结果证实,采用MTT方法、流式细胞术、Caspase3和Caspase7活性检测鼻咽癌肿瘤细胞增殖和凋亡的变化。结果 免疫组织化学结果显示PNCK蛋白在鼻咽癌癌组织中呈特异性高表达。Real time PCR和免疫印迹结果证实慢病毒成功干扰鼻咽癌CNE-2Z细胞中PNCK表达(PNCK基因在mRNA和蛋白水平表达受到显著抑制)。MTT检测结果显示抑制PNCK表达抑制CNE-2Z细胞增殖。此外,干扰PNCK表达引起CNE-2Z细胞Caspase3和Caspase7活性上升,流式细胞术表明干扰PNCK基因表达可以促进CNE-2Z细胞凋亡。结论 干扰PNCK基因表达抑制鼻咽癌细胞增殖并诱导肿瘤细胞凋亡,可能是治疗鼻咽癌的潜在靶点。     

GFP报道基因在鼻咽癌细胞系CNE-2Z中的表达

目的　观察GFP报道基因在鼻咽癌细胞系CNE 2Z中的表达。方法　将带有GFP报道基因的载体质粒pPAT GFP和 3种带有GFP报道基因及目的基因抗端粒酶核酶基因teloRZ的不同质粒 pGFPuro teloRZ 2 .1、pGFPuro teloRZ 7.1、pGFPuro teloRZ 7.7,转染到鼻咽癌CNE 2Z细胞系中 ,用荧光显微镜及流式细胞仪检测转染细胞在固定后的活细胞中GFP基因的表达情况。结果　GFP基因在活细胞中有表达 ,而在固定后无表达 ,且目的基因的插入影响GFP基因的表达。结论　GFP基因在转染了含目的基因质粒的鼻咽癌CNE 2Z细胞中稳定表达 ,可以在CNE 2Z细胞株和抗端粒酶模板区核酶的研究中作为质粒转染和基因表达的报道基因     

赫赛汀对鼻咽癌细胞株体内外生长的影响

 目的 探讨赫赛汀（Herceptin）对鼻咽癌细胞株体内外生长的影响. 方法 采用免疫组化技术检测鼻咽癌组织HER-2/neu基因表达;采用3H-TdR细胞掺入法测定Herceptin对鼻咽癌细胞株体外生长的影响;用荷瘤裸鼠模型观察Herceptin对鼻咽癌细胞株体内生长的影响. 结果 HER-2/neu基因在鼻咽癌组织中有过表达; Herceptin可抑制HER-2/neu基因过表达的鼻咽癌细胞株CNE-2Z 3H-TdR掺入和影响荷瘤裸鼠肿瘤的生长. 结论 Herceptin可抑制CNE-2Z细胞株体外3H-TdR掺入,并影响CNE-2Z细胞株体内的生长.