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采用碳二亚胺法将经过羧基化衍生的5-氟尿嘧啶连接到花生凝集素上,制备一种导向肿瘤的5-氟尿嘧啶-花生凝集素结合物,并应用改进的三硝基苯磺酸法测定药物结合率。测得的药物结合率为76.33%。结果表明碳二亚胺法可成功实现药物与花生凝集素的连接,该法制备得到的结合物其药物结合率高。 相似文献
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目的观察5-氟脲嘧啶与γ-干扰素联合应用对人结肠癌Lovo细胞株的影响。方法采用Lovo结肠癌细胞体外培养,流式细胞仪检测细胞生长、增殖和凋亡的变化。结果 5-氟尿嘧啶与γ-干扰素联合应用后,细胞增殖率下降指数为0.82~1.99,而细胞凋亡率增加指数为2.48~4.89。结论 5-氟尿嘧啶与γ-干扰素联合应用可降低肿瘤细胞的增殖和提高肿瘤细胞的凋亡。 相似文献
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目的建立荧光定量-逆转录-聚合酶链反应(FQ-RT-PCR)测定TH细胞中IL-2 mRNA和IL-4 mRNA方法,并作临床初步应用。方法制备IL-2 cDNA和IL-4 cDNA,分别构建含有人IL-2基因和叫基因片段的pMD18-T质粒,克隆后作为定量阳性模板;设计和制备用于FQ-RT-PCR的引物和FAM、TAMRA标记的探针,优化实验条件,建立FQ-RT-PCR方法;用固相单抗方法从健康人、妇科良性疾患和恶性肿瘤患者以及慢性肾功能衰竭(cRF)患者PBMC中富集CD4(TH)细胞,经PMA和calcium ionophore诱导,提取总RNA,继用所建FQ-RT-PCR方法,作IL-2 mRNA和IL-4 mRNA定量测定。实验设阻actin为内控基因以监测RNA的质量。结果根据系列模板浓度的对数与CT值作直线回归建立标准曲线,线性范围为10^2~10^7copies/μl;不精密度试验显示,高值样品的批内、批间CV分别为7.8%和12.5%,低值样品的批内、批间CV分别为10.8%和19.5%;妇科恶性肿瘤患者与健康对照组和妇科良性疾患组比较,CRF患者与健康对照组比较,IL-2 mRNA表达均显著降低,IL-4 mRNA表达均显著升高(P〈0.001)。结论用所建立的FQ-RT-PCR法可对TH细胞内IL-2 mRNA和IL-4 mRNA作定量测定,方法简便、敏感、准确;妇科恶性肿瘤患者和CRF患者TH细胞内IL-2mRNA和IL-4 mRNA表达有明显的极化现象,呈TH2反应,提示恶性肿瘤患者和CRF患者TH细胞功能处于失衡状态。可通过改善和调整TH细胞的失衡提高恶性肿瘤患者的免疫监视功能和纠正CRF患者免疫紊乱。 相似文献
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用于组织切片透明的二甲苯经长时间使用后,会混入大量的无水乙醇、组织碎屑及少许伊红染料,尤其是夏季天气潮湿还会吸收水分,液体呈淡红色混浊状,染色缸底部会有小水珠。此时二甲苯的透明作用明显减弱,致使组织切片透明不彻底,切片模糊不清,造成诊断困难,甚至误诊。通常解决的办法是将旧液倒掉,换新液。因目前二甲苯售价较贵,为节约试剂、降低消耗、减少环境污染,我们根据乙醇溶于水而二甲苯不溶于水的特性,经反复试验,探索出用水提取旧二甲苯内的无水乙醇和水,从而提纯旧二甲苯的方法。 相似文献
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目的:探索用星形测试卡测试屏-片系统的调制传递函数(modulation transfer function,MTF)的方法,获得随空间频率连续变化的屏-片系统的MTF曲线。方法:把星卡的X射线照片用显微密度计分别沿黑、白楔条的角平分线方向进行扫描,将得到的数据用计算机处理,获得屏-片系统的MTF曲线。并将该方法得到的MTF曲线与用矩形波测试卡得到的MTF曲线进行比较。结果:通过星卡曝光一微密度计扫描的方法,测试得到了随着空间频率的增加而逐渐减小的屏-片系统的MTF曲线,曲线随着空间频率的变化而连续变化。结论:用星形测试卡可以获得随空间频率连续变化的屏-片系统的MTF曲线;并且用星形测试卡测试得到的MTF曲线,在空间频率的连续性上明显优于用矩形波测试卡得到的MTF曲线。 相似文献
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本文建立了白细胞介素-10(IL-10)放射免疫分析法(RIA)并初步应用于临床.将重组IL-10作免疫原,获多克隆抗体.采用Iodogen法制备高比活度125I-IL-10,建成IL-10 RIA.应用本法测定抑郁症患者组及对照组血清IL-10含量,结果表明:IL-10测定范围为5-1215ng/mL;对照组血清IL-10含量为28.0±8.9ng/mL,而抑郁症患者组IL-10含量为25.3±11.1ng/mL,较对照组低(P《0.05);患者治疗后(28.4±13.2ng/mL)较治疗前有明显升高(P《0.05).IL-10 RIA的建立为进一步开展IL-10在相关学科的研究,提供了一种有价值的指标. 相似文献
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目的研究集中载荷作用下椎体-椎间盘的固液耦合问题。方法将椎体-椎间盘视为多孔介质材料,包含固体骨架和隙间流体两相,采用Biot固液耦合方程描述两相影响过程。建立固相平衡方程与液相连续性方程的轴对称表达形式,应用加权残值法得到耦合方程轴对称表达的等效积分形式,离散后得到有限元格式,对时间的离散采用隐式向后差分法。应用有限元程序对椎体-椎间盘的轴对称模型进行数值分析。结果在集中力作用下,椎体-椎间盘孔隙压力随着时间的增大而减小,同时位移随着时间的增大而增大,逐渐两者趋于平衡;压力与位移在力作用点处最大,以此为中心向四周递减。结论载荷对孔压和位移有显著影响;计算结果与现有文献结果基本相符。 相似文献
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BACKGROUND: Interstitial fluid flow around the osteocytes caused by a series of physiological activities plays an important role in the osteocyte metabolism and signal transduction.
OBJECTIVE: To review and summarize the research progress of bone structures and physiological functions, then further illustrate how the mechanical loads make an impact on bone tissue.
METHODS: A computer-based search was conducted in PubMed database for articles related to the bone microstructure, metabolism and osteocyte signal transduction published from January 2009 to December 2015. The keywords were “osteocyte, mechanical load, permeability, lacunar-canalicular system, interstitial fluid flow, mechanotransduction, signaling pathways” in English. Data were screened firstly, dated articles or literatures with wrong research methods were excluded, and totally 40 eligible articles were enrolled.
RESULTS AND CONCLUSION: Bone tissue can quickly adapt to the change of mechanical environment to guarantee enough osteocytes in the functional bone area indicating that osteocytes regulate the bone absorption and formation by responding to stress, which is closely related to osteocyte physiological characters. Osteocytes exist in mineralized matrix, and the special microstructures make it possible to receive mechanical loads and transform the mechanical signals into chemical signals aimed at regulating the bone absorption or formation.So the interstitial fluid flow in the lacuna-canalicular system occures, namely load-induced fluid flow, when the mechanical load is distributed on the bone. This fluid flow affects the bone tissue through two mechanisms: regulating osteocyte metabolism and participating in the mechanotransduction. In conclusion, the mechanical load plays a vital role in maintaining health bone and regulating bone adaptation.
中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程 相似文献
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目的 评估两种α-淀粉酶检测的常规方法与参考方法的一致性,旨在为临床提供准确结果。方法 采用血清α-淀粉酶参考方法和临床常规方法同时测量ERM-DA456、GBW(E)090595、GBW(E)090286、42份单人份血清样本,参考CLSI EP9-A3文件,将两种常规方法检测结果分别与参考方法进行比对;参考EP14-A3文件,对常规方法使用的校准品进行互换性分析。结果 参考方法和迈克系统标物测量结果的偏倚均小于临床可接受偏倚7.5%;西门子系统除ERM-DA456/IFCC外均大于临床可接受偏倚。迈克系统Bland-Altman图说明其与参考方法的结果一致,各医学决定水平处的偏倚也均小于临床可接受偏倚;校准品有互换性。西门子系统Bland-Altman图虽然95%以上数据落在偏倚均值±2SD范围内,但百分比偏倚均为负值,说明其有系统偏差;其在医学决定水平50U/L处的偏倚均大于临床可接受偏倚;校准品无互换性。结论 迈克系统与参考方法有较好的一致性,其检测结果更准确可靠。 相似文献
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Qingfeng Yin Qianqian Zhai Di Wang Jie Hai Meng Cao Jianling Wang Tao Wang 《International journal of clinical and experimental pathology》2015,8(11):15216-15221
We conducted a case-control study to investigate the association between interleukin (IL)-10-592C/A, -819C/T and -1082A/G polymorphisms and susceptibility to diabetic nephropathy. A hospital-based case-control study was taken in our study. A total of 172 patients with proven type 2 diabetes mellitus and 344 controls were recruited from the First Affiliated Hospital of Xinxiang Medical University between March 2012 and October 2014. Genotyping of IL-10 -592C/A, -819C/T and -1082A/G polymorphisms was done by done by PCR-RFLP methods. By the χ2 test, the distributions of the GG, GA and AA genotypes in IL-10 -1082A/G were significantly different between patients with diabetic nephropathy and control subjects (χ2 = 8.09, P = 0.02). By conditional logistic regression analysis, we found that the AA genotype of IL-10 -1082A/G was associated with an elevated risk of diabetic nephropathy compared to the GG genotype in codominant model, and the adjusted OR (95% CI) was 2.38 (1.23-4.57). In dominant model, the GA+AA genotype was associated with a significantly increased risk of diabetic nephropathy compared to the GG genotype in dominant model (OR = 1.47, 95% CI = 1.05-2.16). In recessive model, the AA genotype could influence the susceptibility to diabetic nephropathy when compared with the GG+GA in recessive model (OR = 2.08, 95% CI = 1.12-3.85). In conclusion, we suggested that IL-10 -1082A/G gene polymorphism was correlated with development of diabetic nephropathy, but no association was observed between IL-10 -819T/C and -592A/C and risk of diabetic nephropathy. 相似文献
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脂氧素对Jurkat T细胞增殖和白细胞介素-2/白细胞介素-2受体表达的影响 总被引:2,自引:0,他引:2
目的:研究脂氧素对Jurkat T细胞增殖和白细胞介素-2表达的影响。方法:体外培养Jurkat T细胞,anti-CD3(2 mg/L)和anti-CD28(2 mg/L)单抗刺激Jurkat细胞活化,加入不同浓度脂氧素(0.1 nmol/L-100 nmol/L)共同孵育24h后,加入[3H]-TdR继续孵育6 h,液闪仪测放射性活度;或收集培养上清,ELISA测IL-2水平,收集细胞,流式细胞仪检测细胞表面IL-2受体α亚单位CD25表达,PI染色后经流式细胞仪进行细胞周期分析。结果:脂氧素剂量依赖性抑制anti-CD3和anti-CD28活化的Jurkat T细胞增殖(P<0.05);细胞周期分析发现脂氧素处理组S期细胞比例减少;脂氧素显著降低培养上清中IL-2 含量(P<0.05)但对CD25表达无明显影响(P>0.05)。结论:脂氧素能抑制活化Jurkat T细胞增殖和白细胞介素-2表达;脂氧素可通过影响T淋巴细胞的活化增殖进而发挥免疫负性调节作用。 相似文献
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目的研究早老素-1基因启动子区-48C/T位点的多态性与迟发性AD(LOAD)的相关性.方法用常规方法从人外周血白细胞中抽提基因组DNA,PCR扩增出包含-48C/T多态性位点的基因片段,利用PCR-RFLP技术对-48C/T多态性位点进行基因分型,χ2检验分析-48C/T多态性位点基因型分布和等位基因频率.结果以33例AD病例和32例对照样品的外周血白细胞基因组DNA为模板,PCR扩增出了长度为344bp的包含-48C/T多态性位点的基因片段.基因分型结果表明,33例散发型AD的C和T等位基因频率分别为47%和53%,C/T和T/T基因型频率分别为94%和6%.而32例正常对照的C和T等位基因频率分别为48%和52%,C/T和T/T基因型频率分别为97%和3%.χ2检验结果显示,病例-对照样品间C和T等位基因频率及C/C、C/T和T/T基因型的分布均无显著性差异(χ2值分别为0.443和0.318,P>0.05).结论在我们研究的群体中,早老素-1基因启动子区-48C/T位点的多态性与LOAD无显著的遗传相关性. 相似文献
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Agnieszka Maria Jeleń Aleksandra Sa?agacka Marta Karolina ?ebrowska Marek Mirowski Monika Talarowska Piotr Ga?ecki Ewa Izabela Balcerczak 《International journal of medical sciences》2015,12(12):974-979
Background: Despite the high prevalence of depression, the mechanism of the origin of this disease as well as the causes of resistance to therapy in some patients are still not fully understood. Increasingly, the possible role of genetic factors is considered. One of them is polymorphisms in the ABCB1 (MDR1) gene which encodes P-glycoprotein, responsible for the transport of xenobiotics, including antidepressant drugs, through the blood-brain barrier.Methods: C3435T was evaluated in 90 patients with recurrent depressive disorders (rDD). Genotyping was performed using a polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP).Results: The obtained results indicate that the TT genotype occurred more frequently among patients with rDD than in healthy volunteers (p=0.0441). Also, at least one C allele was present significantly less frequent in the study group than in healthy individuals (p=0.0300). The severity of depressive symptoms was higher among patient with the CC genotype in comparison with the other genotypes (p=0.0106) but treatment response to antidepressants was better in this group than among patients with CT or TT genotypes (p=0.0301). Likewise, patients with the T allele have a significantly lower severity of symptoms (p=0.0026) and decreased therapy effectiveness (p=0.0142) than C allele carriers.Conclusions: This study suggests that C3435T polymorphisms in the ABCB1 gene are strongly associated with a predisposition to depression development, the severity of depressive symptoms and the effectiveness of therapy with using different groups of antidepressant agents. 相似文献
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目的 探讨高迁移率族蛋白B1(high modility groupbox 1,HMGB1)抑制剂对脓毒症T淋巴细胞和单核细胞的作用机制。 方法 将30只C57BL/6雄性小鼠随机分为3组,每组10只,依次为假手术组、模型组、抑制剂组。模型组、抑制剂组采用盲肠结扎穿孔手术构建脓毒症小鼠模型,假手术组小鼠仅暴露盲肠后即进行伤口缝合,不结扎。抑制剂组小鼠术后腹腔注射HMGB1特异性抑制剂甘草酸(10 mg/kg),每6 h注射1次,连续4次,假手术组和模型组腹腔注射等剂量的生理盐水。处死小鼠后,无菌分离小鼠的胸腺组织,常规提取胸腺中的T淋巴细胞和单核细胞。MTT法与流式细胞术测定T淋巴细胞的增殖活性和凋亡情况,Transwell趋化实验与ELISA法测定单核细胞的趋化活性以及分泌炎性因子TNF-α、IL-6及IL-10的情况,Western blot检测胸腺组织中HMGB1和第10号染色体同源丢失性磷酸酶-张力蛋白(phosphatase and tensin homolog deleted onchromosometen,PTEN)的表达水平。 结果 与假手术组相比,模型组小鼠胸腺组织T淋巴细胞的增殖活性和凋亡率、单核细胞的趋化活性和TNF-α、IL-6、IL-10蛋白的表达、HMGB1和PTEN的表达明显升高(均P<0.05);与模型组相比,抑制剂组小鼠胸腺组织T淋巴细胞凋亡率、TNF-α、IL-6蛋白的表达、HMGB1和PTEN的表达明显降低,T淋巴细胞的增殖活性、单核细胞的趋化活性、IL-10蛋白的表达明显升高(均P<0.05)。 结论 HMGB1抑制剂可降低脓毒症小鼠胸腺T细胞的凋亡率,增强T细胞的增殖活性和单核细胞的趋化活性,提高单核细胞抗炎因子IL-10的分泌,抑制促炎因子TNF-α、IL-6的分泌。 相似文献
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刘凌霜 《中国健康心理学杂志》2016,(12)
目的:通过新媒体视阈下思想政治理论课(以下简称思政课)学习方式、学习投入和学业自我效能感之间的关系调查和实证分析,探讨大学生思政课学习心理和学习效能问题。方法:采用自制新媒体学习方式与大学生思政课学习效能的调查问卷、大学生学习投入量表和学业自我效能感问卷,对579名大学生进行问卷调查。结果:1自制问卷的内部一致性达到0.832,KMO检验系数r0.8(P0.001),说明自制问卷信度和效度较高,可以作为进一步研究的工具;2大一学生学习投入的动机维度得分显著高于大二学生(t=2.64,P0.01);3学习方式总均分及其体验和评价两个维度与学业自我效能感的学习能力维度之间存在显著正相关(r=0.102,0.096;P0.05;r=0.112、P0.01);学习投入总均分及其3个维度与学业自我效能感总均分及其两个维度之间也存在显著正相关(r=0.20,0.22,0.24;P0.01);4大一学生学习方式和学习投入对思政课学业自我效能感具有显著的回归效应(R=0.423,Beta=0.115,P0.05,Beta=0.405,P0.001),能联合解释学业自我效能感17.9%的变异量。结论:新媒体视阈下学习方式、学习投入和思政课学业自我效能感之间的关系调查和实证分析,对深化思政课教学改革具有积极的理论和现实意义。 相似文献
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共刺激分子4-1BB和4-1BB配体(4-1BBL)属于肿瘤坏死因子/肿瘤坏死因子受体(TNF/TN—FR)超家族的重要成员,分别表达在活化的T细胞及树突状细胞(DC)上。4-1BB与4-1BBL相互作用产生的共刺激信号能够促进T细胞增殖、分化以及细胞因子的产生。4-1BB/4—1BBL信号途径在自身免疫性疾病、肿瘤、病毒感染等疾病的发生、发展过程中起着重要的免疫调节作用。干预调节4-1BB/4-1BBL信号途径有望为疾病的免疫治疗提供新的思路。 相似文献
18.
目的 克隆并构建含小鼠4-1BB胞外段和人IgG Fc融合基因的真核表达质粒,表达具有生物学活性的4-1BB-Fc融合蛋白,初步探讨其体外生物学效应。方法 借助RT-PCR技术,从小鼠脾脏总FLNA中扩增4-1BB全长编码基因,并导入克隆载体pGEM-T Easy。经测序证实,用PCR扩增其膜外区cDNA,酶切后与hIgG1 Fc基因一起装入真核表达载体pcDNA3.1中。应用脂质体将重组子pcDNA3.1-4-1BB-Fc转染COS-7及CHO细胞,经G418筛选,获得稳定表达4-1BB-Fc融合蛋白的CHO细胞株。双抗体夹心ELISA检测融合蛋白表达,经蛋白A亲和层析纯化,借助免疫印迹进行鉴定。应用FACS检测融合蛋白与DC细胞系DC2.4表面4-1BBL结合情况。采用MTT及CFSE(羧基荧光素乙酰乙酸)标记法检测4-1BB-Fc融合蛋白对同种异基因小鼠T细胞增殖的抑制作用。结果 经测序证实,所克隆和构建的小鼠4-1BB-Fc cDNA阅读框及连接部位序列正确;ELISA与免疫印迹证实4-1BB-Fc蛋白的表达及其对T细胞增殖具有明显抑制作用。结论 成功构建4-1BB-Fc融合基因并获稳定表达,可望用于探讨4-1BB参与移植排斥的作用及其机制。并为研究4-1BB-Fc的其他生物学作用奠定初步基础。 相似文献
19.
Shu-Xu Du Yong-Rui Jia Si-Qi Ren Xiao-Jun Gong Hong Tang 《Advances in medical sciences》2018,63(1):134-139
Purpose
we studied the effect of Bacillus licheniformis preparation (ZCS) on CNST (central nervous system tumor) patients undergoing the gastrointestinal symptoms and inflammation induced by radiotherapy.Materials and Methods
160 CNST patients with craniospinal irradiation (CSI) treatment were divided into experiment and control group. The experiment group patients took one capsule per time of ZCS and three times a day until the end of radiotherapy, starting one day before radiotherapy. While the patients in control group were administrated placebo without any probiotics. Serum from one day before radiotherapy and the first day after radiotherapy were collected to measure the ET, CRP, TNF-α, IL-1β and IL-6.Results
More than 70% CNST pediatric patients suffered from different degrees of gastrointestinal symptoms after radiotherapy, including mouth ulcer, nausea, vomiting, abdominal pain and diarrhea. And there was an obviously increased of serum ET, TNF-α, IL-1β, IL-6 and CRP after RT. Importantly, a markedly decreased of ET, CRP and inflammatory cytokines were detected in the experiment group comparing to the control group after radiotherapy, as well as the relief of the gastrointestinal symptoms. However, improvement of probiotics (or ZCS) of the survival rate of CNST children and the recurrence of tumor are not observed in this study.Conclusions
Prophylactically administrated ZCS during radiotherapy for CNST patients can relieve RT-related gastrointestinal symptoms and inflammatory reaction. 相似文献20.
目的 检测乙型肝炎病毒( HBV)感染者外周血CD4+T细胞表面CD25 - CD127 -的表达情况及临床意义.方法 用流式细胞术比较分析53例慢性乙肝患者、53例HBV携带者和26例正常对照人群CD4+T细胞表面CD25 - CD127 -的表达情况,并对20例用干扰素治疗的HBV-DNA阳性慢性乙肝患者随访.结果 ①与正常对照组比较,慢性乙肝患者、HBV携带者CD4+ CD25 -CD127 -T细胞均显著升高,两者比较有统计学差异(Q =4.559,P<0.05;Q=6.230,P<0.05);②HBV- DNA阳性患者(n=77) CD4+ CD25 - CD127 -T细胞显著低于HBV- DNA阴性患者(n=29),两者比较有统计学意义(t =2.290,P=0.024);③与治疗前比,慢性乙肝患者干扰素治疗12周后CD4+CD25- CD127 -T细胞显著降低,两者比较差异有统计学意义(t =2.469,P=0.024).结论 乙型肝炎病毒感染者外周血CD4+ CD25 - CD127 -T细胞与病毒的感染和清除相关,外源性干扰素可降低CD4+ CD25 - CD127 -T细胞. 相似文献