淫羊藿提取物对犬急性心肌缺血的保护作用

目的观察淫羊藿提取物对犬急性心肌缺血的保护作用。方法取杂种犬24只,随机分为4组(每组6只),分别为生理盐水对照组,阳性对照药物(地奥心血康胶囊)组,52.0 mg/kg淫羊藿提取物组和104.0 mg/kg淫羊藿提取物组;采用结扎麻醉,犬开胸后结扎左冠状动脉前降支制备实验性急性心肌缺血模型,观察淫羊藿提取物对心外膜电图、心肌梗死面积和血清酶的影响。结果淫羊藿提取物减少缺血程度,降低缺血范围,缩小缺血心肌的梗死面积,降低血清中磷酸肌酸激酶、乳酸脱氢酶的活性,降低血清中游离脂肪酸、过氧化脂质含量,提高超氧化物歧化酶、谷胱甘肽过氧化物活性。结论淫羊藿提取物对实验性心肌缺血犬心肌产生保护作用。     

p38MAPK途径在淫羊藿甙促成骨细胞分化中的作用

观察淫羊藿甙对体外培养新生大鼠颅盖骨成骨细胞p38丝裂原活化蛋白激酶(MAPK)蛋白表达的影响,探讨淫羊藿甙防治骨质疏松症的信号转导机制.结果 显示淫羊藿甙可以在5 min内激活p38MAPK途径,并且可以上调Cbfa1蛋白表达和活性升高,加用p38MAPK途径的抑制剂SB203580后可以部分抑制淫羊藿甙对Cbfa1蛋白和活性的上调作用.

Abstract：

This paper was aimed to investigate the effects of icariin on the expression of p38 mitogen activated protein kinase(MAPK)protein in rat osteoblasts cultured in vitro, to elucidate the signal transduction of icariin in preventing and treating osteoporosis. The results showed that p38MAPK could be activated by icariin within 5min, and Cbfal could also be upregulated, and SB203580, an inhibitor of p38MAPK pathway, could partly inhibit Cbfal upregulation by icariin.     

淫羊藿甙对P8系快速老化小鼠记忆障碍的改善作用

目的 观察淫羊藿甙对P8系快速老化小鼠(SAMP8)记忆能力的影响.方法 6月龄SAMP8随机分为治疗组和模型组,每组10只;另以10只同月龄R1系正常同源抗快速老化小鼠SAMR1为对照组.治疗组以淫羊藿甙灌胃,模型组及对照组以等剂量双蒸水灌胃.8w后,运用Morris水迷宫方法测试SAMP8的定位航行和空间探索能力.结果 治疗组寻找水下平台的潜伏期较模型组显著缩短(P＜0.05),跨平台象限游泳次数较模型组显著增多(P＜0.05).结论 淫羊藿甙能有效改善SAMP8的记忆障碍.     

淫羊藿甙对原代培养神经元缺氧缺糖损伤的保护作用

目的 探讨淫羊藿甙对体外原代培养神经元缺氧缺糖损伤的作用。方法 原代培养大鼠乳鼠皮质神经元,在缺氧缺糖4、6、8 h时检测正常对照组、损伤模型组、淫羊藿甙处理组的神经元细胞噻唑蓝、乳酸脱氢酶(LDH)病理学指标变化。结果 模型组在缺氧缺糖4、6、8 h后噻唑蓝值明显低于相应对照组(P<0.01),LDH漏出率明显高于对照组(P<0.05或<0.01)。淫羊藿甙组与相应模型组比较,可提高缺氧缺糖的神经元生长能力,使噻唑蓝值有所上升;LDH漏出率有所下降;改善细胞形态。 结论 淫羊藿甙对体外原代培养神经元具有保护作用,可减轻神经元缺血性损伤。淫羊藿甙有利于缺血性脑血管疾病的治疗。     

淫羊藿甙对大鼠成骨细胞护骨素、RANKL表达的影响

目的观察淫羊藿甙对体外培养大鼠成骨细胞护骨素(OPG)、核因子-kB受体活化因子配体(RANKL)mRNA表达的影响。方法用酶消化法分离24h内新生SD大鼠颅盖骨成骨细胞,进行原代培养。在培养液中加入不同浓度的淫羊藿甙(10~(10)～10~(14)mol/L),培养48h后,抽提总RNA,采用半定量RT-PCR法检测成骨细胞中OPG和RANKL mRNA表达的变化。结果淫羊藿甙可明显促进成骨细胞OPG mRNA的表达,抑制RANKL mRNA的表达,且呈浓度依赖性,均以10~(-7)mol/L时作用最显著(P＜0.05)。预先用淫羊藿甙干预成骨细胞,可逆转地塞米松的降低OPG mRNA(0.570±0.093vs 1.083±0.081)、升高RANKL mRNA(1.079±0.050vs 0.718±0.082)的作用(均P＜0.05)。结论淫羊藿甙可以上调OPG基因表达,下调RANKL基因表达,从而调节骨吸收,这可能是淫羊藿甙防治骨质疏松症的重要机制之一。     

淫羊藿甙对去卵巢大鼠骨组织核心结合因子α1表达的影响

目的 观察淫羊藿甙对去卵巢大鼠骨组织Cbfa1表达的影响.方法 54只雌性SD大鼠随机分成6组9只/组:假手术组、模型组、尼尔雌醇组、淫羊藿甙低剂量组、淫羊藿甙中剂量组和淫羊藿甙高剂量组.对尼尔雌醇组和淫羊藿甙低、中、高剂量组分别给予尼尔雌醇(0.1mg/kg)和淫羊藿甙(40,80和160mg/kg)治疗12周.12周后,以DXA法测定大鼠全身的骨密度;放射免疫法测定各组大鼠血清中骨钙素及雌二醇的浓度;处死各组大鼠,直接从头盖骨中提取总.RNA,采用荧光定量PCR技术检测Cbfa1 mRNA的相对表达量.结果 12周后,模型组大鼠的骨密度和血清雌二醇水平明显降低,与假手术组相比,差异有显著性(P<0.05).与模型组相比高剂量组的全身骨密度和血清骨钙素含量增加(P<0.05),但是血清雌二醇的水平无变化(P>0.05).12周后,与假手术组相比,模型组大鼠骨组织中Cbfal mRNA表达明显降低,差异有显著性(P<0.01).与模型组相比高剂量组骨组织中Cbfal mRNA的表达,显著升高(P<0.01).结论 淫羊藿甙对去卵巢大鼠骨质疏松症具有防治作用,其部分机制可能为淫羊藿甙促进骨组织中Cbfa1表达,从而调节骨形成.     

淫羊藿次甙Ⅱ对小鼠成骨细胞护骨素表达的影响

以17β-雌二醇为阳性对照组,观察不同浓度淫羊藿次甙Ⅱ对成骨细胞护骨素(OPG)表达的影响,结果发现20.ng/dl淫羊藿次甙Ⅱ上调成骨细胞OPGmRNA的表达,且优于阳性对照组(P<0.05),淫羊藿次甙Ⅱ也显著增加成骨细胞OPG分泌水平,20 ng/dl淫羊藿次甙Ⅱ组的促分泌活性显著优于阳性对照组,提示淫羊藿次甙Ⅱ作为淫羊藿甙在体内的主要代谢产物,在体外较低剂量即可显著促进成骨细胞OPG的表达,具有良好的抗骨质疏松作用.

Abstract：

To explore the effect of icariside ? on osteoprotegerin(OPG) expression in osteoblasts, primary osteoblasts were cultured with different concentration of icariside ? and 17?estradiol. As a major metabolite of icarrin in vivo, icariside ? exerted a better effect on OPG expression which plays a key role in osteoporosis. 20 ng/dl icariside ? also exerted better effect on OPG secretion than that of positive control group. Consequently, icariside ?could be a candidate for osteoporosis treatment.     

淫羊藿苷对老年大鼠的镇痛作用

目的 探讨不同浓度的淫羊藿苷(ICa)对老年大鼠痛觉调节的影响.方法 采用浓度为3×10-6g/ml和6×10-6g/ml ICa灌胃,利用热板与压板测痛仪记录大鼠后爪缩爪反应潜伏期(HWL),观察ICa对大鼠中枢神经系统痛觉调节的影响.结果 与灌生理盐水的正常大鼠(对照组)比较,3×10-6g/ml的ICa热板没有显著差异(P＞0.05),而3×10-6g/ml的ICa压板、6×10-6g/ml的ICa热板及压板均具有显著差异(P＜0.05；P ＜0.01或P＜0.001),表明ICa对大鼠具有明显的镇痛作用.结论 ICa对大鼠的痛觉具有调节作用,且具有剂量依赖性.     

淫羊藿、肉苁蓉、巴戟天对果蝇寿命影响的研究

目的研究淫羊藿、肉苁蓉、巴戟天3种药物对果蝇寿命的影响。方法采用在实验组的培养基中适量加入上述的3种药物，而在对照组的培养基中则不加药物，观察各组的生存时间。结果实验组果蝇的平均寿命高于对照组果蝇的平均寿命。结论适量应用淫羊藿、肉苁蓉、巴戟天，具有延缓衰老的功效。     

淫羊藿提取物干预阿尔茨海默病病理发生机制的作用

<正>淫羊藿是小檗科淫羊藿属多年生草本植物,始载于《神农本草经》,是安神、健脑、补肾、治疗脑疾病的首选药材。经实验证明,淫羊藿提取物具有抗氧化应激、减轻炎症反应、调节神经递质、减少淀粉样蛋白(Aβ)生成、抑制神经细胞凋亡及改善阿尔茨海默病(AD)患者的认知功能等多种神经生物学功能[1]。本文就淫羊藿提取物有效成分对AD的病理发生机制的可能作用做一综述。1化学成分研究淫羊藿中含有多种具有生理活性的化学成分。淫羊藿属     

克山病病区粮及维生素E和硒对雄性大鼠某些内分泌激素的影响

本研究给雄性大鼠饲喂克山病病区粮,以及补加维生素 E(VE)和/或硒,观察其血清促甲状腺激素(TSH)、催乳素(PRL)、促黄体激素(LH)、卵泡刺激素(FSH)及睾丸酮(TS)水平变化。结果表明,饲喂克山病病区粮,大鼠血清 TSH 水平明显增高(P<0.01),血清 PRL 水平轻度增高(P>0.05),而血清 LH、FSH 和 TS 水平轻度降低(P>0.05);补加 VE和/或硒,减轻了饲喂病区粮而引起的上述各指标改变。提示,饲喂克山病病区粮,可能直接影响垂体前叶 TSH 细胞,增强甲状腺轴的功能,而对垂体前叶 PRL 细胞及性腺轴的影响不十分明显;向病区粮补加 VE 和/或硒,可减轻其病区粮的上述影响。VE 缺乏,如同低硒,在克山病发病中起到重要作用。     

不同不育类型患者精液质量比较及对补救卵胞浆内单精子注射术临床结局的影响分析

目的 探讨不同不育类型患者精液质量情况及其对补救卵胞浆内单精子注射术(R-ICSI)临床结局的影响.方法 回顾性研究2014-01～2018-12于广西壮族自治区人民医院生殖医学与遗传中心行常规体外受精-胚胎移植(IVF-ET)失败而进行R-ICSI治疗的98对不孕不育夫妇的临床资料.根据不育类型将98对夫妇分为原发性...     

Preliminary investigation about the expression of tubulin in platelets from patients with iron deficiency anemia and thrombocytosis

Objective: In order to inquire into the pathogenesis of increased platelet counts in peripheral blood of patients with iron deficiency anemia (IDA), the phenomenon of thrombocytosis was confirmed, and then the expression of tubulin within platelets from IDA patients was investigated.

Methods: Peripheral blood samples were collected from 79 patients with IDA and were divided into 2 groups, group of IDA with normal platelet counts (34 cases), and group of IDA with increased platelet counts (thrombocytosis) (45 cases). Additionally, 45 peripheral blood samples from healthy volunteers were enrolled as a group of healthy controls. Count of platelets in peripheral blood was detected by means of LH-780 hematology analyzer and hemocytometer under a microscope respectively, and analyzed statistically.

Results: There was no statistical difference between platelet counts detected by LH-780 hematology analyzer and hemocytometer under a microscope (P?>?.05). The mean fluorescence intensity (MFI) of both α-tubulin and β-tubulin within platelets from IDA patients with thrombocytosis was significantly less than that from healthy volunteers and IDA patients with normal platelet counts (P?P?>?.05).

Conclusion: Some patients with IDA are accompanied by thrombocytosis, from which the expression of α-tubulin and β-tubulin within platelets reduced obviously compared with those with normal platelet counts and healthy controls respectively. It is implied that downregulation of tubulin probably is a part of the pathogenesis leading to increased platelet counts in IDA.     

润肺口服液对慢性支气管炎大鼠支气管TNF-α、ICAM-1蛋白及IL-8表达的影响

目的研究中药复方润肺口服液对慢性支气管炎（CB，CB）支气管肿瘤坏死因子（TNF-α）、细胞间黏附分子-1（ICAM-1）及白介素-8（IL-8）表达的影响，探讨其治疗CB的疗效机制。方法雄性Wistar大鼠随机分为假手术组、慢性支气管炎组、慢性支气管炎氢化可的松治疗组及慢性支气管炎润肺口服液治疗组，每组8只。大鼠慢性支气管炎模型通过熏香烟加气管内注入低剂量脂多糖制成，利用免疫组织化学观察各组大鼠支气管TNF-α、ICAM-1蛋白及IL-8的表达情况。结果熏香烟加气管内低剂量脂多糖注射可复制出较理想的慢性支气管炎模型，假手术组支气管上皮内可见TNF-α、ICAM-1蛋白及IL-8弱阳性表达；慢性支气管炎组支气管TNF-α、ICAM-1蛋白及IL-8在支气管上皮细胞、支气管周围的淋巴滤泡炎性细胞和肺泡间质细胞中可见强阳性表达；半定量图像分析显示，慢性支气管炎组TNF-α、ICAM-1蛋白及IL-8的表达明显强于假手术组（P〈0．05），慢性支气管炎润肺口服液治疗组的表达则明显弱于支气管炎组（P〈0．05）。结论润肺口服液通过下调支气管上皮细胞中TNF-α、ICAM-1蛋白及IL-8的表达，从而减轻气道炎症反应是其治疗慢性支气管炎的机制之一。     

抑制TIMP-1的硫代反义寡核苷酸在肝纤维化大鼠体内生物学分布及药代动力学研究

目的　研究抑制TIMP 1的硫代反义寡核苷酸 (S asON )在肝纤维化大鼠体内的生物学分布及药代动力学 ,为其在临床上应用提供理论依据。方法　合成互补于TIMP 1基因的反义寡核苷酸并加以硫代磷酸化修饰 ;用γ 3 2p ATP标记 2 0聚S asON ,给实验大鼠尾静脉一次性注射 ,每 2 4h收集尿液和粪便 ;分别在 12个时间点上放血杀死实验大鼠 ,取各器官组织匀浆 ,用液体闪烁计数测定cpm值。结果　S asON广泛分布于全身各器官 ,以肝、肾聚积浓度最高 ,其药物能在体内维持 72h以上。血浆药物清除曲线符合二室模型。单位时间内机体清除药物的能力即血浆清除率为0 .0 7ml/h ,尿液为主要排除途径 ,2 4h排出 2 8.4% ,72h排出 44 .8% ,粪便排出量较少。结论　抑制TIMP 1的S asON在实验大鼠体内以肝、肾为主的广泛组织生物学分布和较长的清除半衰期 ,使其成药及临床用药变为可能 ,从而为研制新一代抗肝纤维化基因治疗药物奠定了实验基础。肝脏作为反义治疗的靶器官有着重要的现实意义。     

Comparison of the effects of budesonide/formoterol maintenance and reliever therapy with fluticasone/salmeterol fixed-dose treatment on airway inflammation and small airway impairment in patients who need to step-up from inhaled corticosteroid monotherapy

BackgroundIf asthma patients fail to achieve symptom control using a medium dose of inhaled corticosteroid (ICS) alone, adding a long-acting β₂ agonist (LABA) is the preferred treatment. We aimed to compare the effect of two widely available ICS/LABA combinations in these patients in real-life conditions: budesonide/formoterol (BUD/FM; Symbicort^®) for maintenance and reliever therapy (SMART) and a fixed dose of fluticasone propionate/salmeterol (FP/SM).MethodsInadequately controlled asthma patients treated with a medium dose of ICS alone, with an Asthma Control Questionnaire (ACQ) score >0.75 and using a short-acting β₂-agonist (SABA) 2–6 occasions/week, were enrolled. Patients were randomized into two groups and treated with two inhalation twice-daily BUD/FM 160/4.5 μg plus as-needed BUD/FM (SMART group, n = 15) or one inhalation twice-daily FP/SM 250/50 μg plus as-needed procaterol (FP/SM group, n = 15) for 8 weeks.ResultsBoth groups showed significant improvement in airway inflammation, pulmonary functions and symptoms from baseline. The SMART group showed significant improvement in the fraction of nitric oxide, ACQ score, rescue medication use and small airway parameter R5–R20 measured by impulse oscillometry compared with the FP/SM group.ConclusionFor stepping up treatment from ICS alone to an ICS/LABA combination, SMART is preferable for controlling asthma symptoms by suppressing airway inflammation and improving small airway impairment compared with a fixed dose of FP/SM. It may be achieved by the property of BUD/FM itself and as-needed use, but the degree of each contribution must be investigated further.