Tumor implantation following laparoscopy using different insufflation gases

Background: Laparoscopic manipulation of malignancies is associated with an increased incidence of metastasis to port sites in experimental models. This study investigated the effect of different insufflation gases on the implantation of a tumor cell suspension following laparoscopic surgery in an established small animal model. Methods: Forty Dark Agouti rats underwent laparoscopy and the introduction into the peritoneal cavity of a tumor cell suspension. The insufflating gas used for each procedure was one of the following gases (10 rats in each group): carbon dioxide (CO₂), nitrous oxide (N₂O), helium, and air. The rats were killed 7 days after surgery, and the peritoneal cavity and port sites were examined for the presence of tumor. Results: Although no significant differences were seen between air, CO₂, and N₂O insufflation groups, tumor involvement of peritoneal surfaces was less likely following helium insufflation. Conclusion: The results of this study suggest that tumor metastasis to port sites following laparoscopic surgery may be influenced by the choice of insufflation gas. In this study, helium was associated with reduced tumor growth.     

The effect of general anaesthesia on the haemodynamic events during laparoscopy with CO2–insufflation

Sixteen women were studied during elective diagnostic laparoscopy with CO2-insufflation to an intraabdominal pressure (IAP) of 2 kPa and Trendelenburg tilt to 30 degrees. They were allocated to either a halothane (Group I) or a balanced (Group II) anaesthesia with relaxation and controlled ventilation. Heart rate (HR), arterial pressure, stroke volume, CO2-elimination, end-tidal CO2 vol.% and total respiratory compliance (TRC) were the parameters measured, and mean arterial pressure (MAP), total peripheral resistance (TPR), stroke index (SI) and cardiac index (CI) were calculated. At maximum haemodynamic strain, SI and CI were on average reduced by 42% in both groups, without significant changes in HR and MAP. TPR increased by 50% in Group I and 100% in Group II. The reduction in SI was related to the changes in TRC. A small increment in CO2-elimination after CO2-insufflation was most pronounced in Group II. SI and CI did not reach the pre-insufflation values after return to the horizontal position and CO2-exsufflation. The haemodynamic differences between the two groups were small compared to the effects of the laparoscopy procedures.     

Experimental study of effect of embolism of different laparoscopy insufflation gases

BACKGROUND AND PURPOSE: Whilst carbon dioxide is the gas generally used for insufflation during laparoscopy, several studies have reported adverse effects specifically associated with its use. These effects may be attributable to chemical, metabolic, or immunologic effects specific to CO2. Because helium is chemically, physiologically, and pharmacologically inert, it has been suggested as a possible substitute insufflation gas. However, there has been concern about the potential implications of venous gas embolism during helium insufflation. The aim of this study was to examine the physiological effect of the intravenous injection of He and CO2 in an experimental model. MATERIALS AND METHODS: Eleven domestic white pigs were randomly allocated to receive multiple intravenous injections of increasing volumes of either CO2 or He gas. Cardiorespiratory function was measured, and the intravenous volumes of gas that resulted in cardiac arrest were determined. RESULT: Cardiorespiratory functional measures returned to normal quicker after CO2 than after He injection. Helium injection quickly overwhelmed the animal's ability to compensate and resulted in death at a lower volume than did CO2 injection. CONCLUSIONS: Gas embolism during He insufflation is more likely to be lethal than is CO2 embolism. This scenario is most likely following Veress needle insertion into a large vein. Therefore, if He is to be used for insufflation during clinical laparoscopy, the possibility of venous injection should be minimized by avoiding Veress needle use. Further investigation of the safety of He insufflation is warranted before a role during clinical laparoscopy can be recommended.     

Laparoscopic peritoneal dialysis catheter implantation using a Tenckhoff trocar under local anesthesia with nitrous oxide gas insufflation

Background

Laparoscopic implantation of peritoneal dialysis catheters has many advantages over conventional methods. The ability to perform laparoscopy with the patient under local anesthesia allows renal failure patients, who ordinarily might not be considered candidates for general anesthesia, an opportunity to undergo this procedure.

Methods

Using local anesthesia and nitrous oxide pneumoperitoneum, 175 catheters were implanted in long musculofascial tunnels under laparoscopic guidance to minimize the risk of catheter migration and flow dysfunction.

Results

Nitrous oxide pneumoperitoneum was well tolerated, allowing all procedures to be safely completed with the patients under local anesthesia. The overall 1- and 2-year catheter survival rates were 92.7% and 91.3%, respectively. The incidence of catheter tip migration and omental entrapment was 1.7% and 2.9%, respectively. Temporary pericatheter leak occurred in 7.4% of cases.

Conclusions

Nitrous oxide insufflation enables safe performance of laparoscopic surgery with the patient under local anesthesia. Patients benefit from a minimally invasive technique with the assurance of obtaining successful long-term catheter function.     

A Glomic Tumour of the Stomach Treated by Laparoscopy

Glomic tumours are rare tumours usually found on the fingertips, particularly the nail-beds, but they can occur anywhere in the body. The first gastric glomic tumour was identified in 1942 and reported with two other cases in 1951 by Key et al. (1) At present, 100 cases of glomic tumour of the stomach have been reported in the literature. We report a case of benign gastric glomic tumour treated by laparoscopic surgery.

This type of tumour is most frequently benign but cases of malignity have been described. The preoperative assessment is important.     

IN VITRO INHIBITION OF TUMOUR GROWTH IN A HELIUM-RICH ENVIRONMENT: IMPLICATIONS FOR LAPAROSCOPIC SURGERY

Background : The recent results of several experimental studies have suggested that tumour implantation after laparoscopic surgery for intra-abdominal malignancy may be partly related to the chemical composition of the insufflation gas used during surgery. These studies have demonstrated that the use of helium as a laparoscopic insufflation agent for cancer surgery results in less tumour implantation and growth at port sites. To further investigate these findings, the present study was performed to compare the growth of cultured tumour cells after exposure to simulated laparoscopic environments, rich in helium, carbon dioxide (CO₂), or air. Methods : A rat mammary adenocarcinoma cell suspension was exposed to a simulated laparoscopic environment for 40 min in one of the following groups: (i) control (atmospheric air, equivalent to a ‘gasless’ laparoscopic environment); (ii) a CO₂-rich environment; and (iii) a helium-rich environment. Cells were then cultured for 18 h and optical density readings were used to assess the number of viable tumour cells at the end of this period. The experiment was performed twice using an identical protocol to ensure consistency in the results. In a further study, pH was continuously measured using an antimony probe during a 40 min insufflation period and for 10 min after insufflation. Results : Cell growth was significantly lower after incubation in the helium-rich environment compared to both the CO₂ and control groups (P < 0.001). There was a significant decrease in pH in the CO₂ group which was not observed during exposure to either air or helium. Conclusions : The inhibition of tumour growth in a helium-rich environment demonstrated by this study, and the reduced incidence of port–site metastases seen in other experimental studies, suggests that the clinical use of helium as an insufflation gas may have important advantages over CO₂.     

Disappearance of intraperitoneal gas following gynaecological laparoscopy

We investigated 25 day case gynaecological laparoscopy patients to determine the duration of the pneumoperitoneum, the rate at which residual gas disappeared and the relative contribution of this gas to pain in the early postoperative period. The volume of gas was calculated from measurements of the subdiaphragmatic gas bubble obtained from an erect chest X-ray. Each patient was X-rayed twice, either immediately prior to discharge and at 24 h post laparoscopy, or at 24 h and 48 h post surgery. Patients were contacted by telephone at 24 and 48 h to confirm fitness to return for the X-ray and at the same time verbal pain and activity scores were recorded. We found that residual gas was almost entirely gone by 48 h following surgery and that it appeared to reduce in an exponential manner. The contribution of this gas to postoperative pain was significant in the first 24 h, but by 48 h it was considerably reduced. We conclude that in day case gynaecological laparoscopy patients, postoperative pneumoperitoneum is short-lived, and that by 48 h its volume and contribution to postoperative pain should be minimal.     

Conservative management of interstitial pregnancy using operative laparoscopy

Interstitial pregnancy has been treated so far mainly by either resection of the cornu or hysterectomy. In this article, two patients with cornual pregnancy were operated upon laparoscopically with an uneventful postoperative recovery. This laparoscopic treatment is simple, does not require extensive operative laparoscopic expertise, and is relatively short and less expensive than laparotomy.We suggest that laparoscopic conservative repair be used to replace cornual resection by laparotomy or hysterectomy in cases of early diagnosed interstitial pregnancy.     

Hemodynamic changes during laparoscopy with positive end–expiratory pressure ventilation

Hemodynamic measurements were performed in 10 healthy women undergoing elective laparoscopy for the investigation of infertility. A standardized anesthetic technique which included the application of positive end-expiratory pressure (PEEP), 0.49 kPa (3.7 mmHg) was utilized. The following variables were studied: cardiac output, stroke volume and left ventricular ejection time (determined non-invasively with impedance cardiography), heart rate, blood pressure, total peripheral vascular resistance and end-tidal carbon dioxide (ET-CO2). The combination of 25 degrees head-down tilt and PEEP ventilation during laparoscopy was associated with a pressure response that restored arterial pressures to essentially pre-anesthetic levels. Net cardiac effects were small. With this regime low pressure 0.7-1.1 kPa (5-8 mmHg) intra-abdominal insufflation with CO2 was associated with only minor cardiovascular changes. There were no indications that 0.49 kPa PEEP during laparoscopy produced adverse cardiovascular effects. The application of PEEP reduced (P less than 0.001) ET-CO2. There was no net increase in ET-CO2 after CO2-insufflation compared to the measurement after induction of anesthesia. This is in contrast to earlier studies without PEEP where a significant net increase in ET-CO2 was reported after CO2-insufflation.     

Postoperative morbidity following gynaecological outpatient laparoscopy. A reappraisal of the service

Fifty-six women having out-patient gynaecological laparoscopies were studied to determine anaesthetic problems and postoperative morbidity. It is concluded that the procedure is safe, and although the postoperative morbidity appears high it is very acceptable to the patient.     

经肛门单孔腹腔镜直肠肿瘤微创切除术

目的：探讨经肛门单孔腹腔镜直肠肿瘤微创切除术的可行性。方法选取2010年06月-2013年06月,直肠内肿瘤6例,肿瘤距肛缘8~15 cm,其中5例为广基直肠管状腺瘤,另1例为管状腺瘤局部癌变,利用单孔腹腔镜经肛门直肠肿瘤微创切除术。结果6例患者均成功实施经肛门单孔腹腔镜肠腔内直肠肿瘤切除术,切缘干净,创面愈合好,随访1~3年无复发。结论利用单孔腹腔镜经肛门直肠肿瘤切除术和经肛门内镜显微手术一样具有优良的术野显露和宽敞的操作空间,具有可行性。     

Metabolic and immunologic consequences of laparoscopy with helium or carbon dioxide insufflation: a randomized clinical study

Background : Previous studies using animal models have demonstrated that carbon dioxide (CO₂) pneumoperitoneum during laparoscopy is associated with adverse physiological, metabolic, immunological and oncological effects, and many of these problems can be avoided by the use of helium insufflation. The present study was performed in patients to compare the effect of helium and CO₂ insufflation on intraperitoneal markers of immunological and metabolic function. Methods : Eighteen patients undergoing elective upper gastrointestinal laparoscopic surgery were randomized to have insufflation achieved by using either helium (n = 8) or CO₂ (n = 10) gas. Intraperitoneal pH was monitored continuously during surgery, and peritoneal macrophage function was determined by harvesting peritoneal macrophages at 5 min and 30 min after commencing laparoscopy, and then assessing their ability to produce tumour necrosis factor‐α (TNF‐α), and their phagocytic function. Results : Carbon dioxide laparoscopy was associated with a lower intraperitoneal pH at the commencement of laparoscopy, although this difference disappeared as surgery progressed. The production of TNF‐α was better preserved by CO₂ laparoscopy, but the insufflation gas used did not affect macrophage phagocytosis. Patients undergoing helium laparoscopy required less postoperative analgesia. Conclusion : The choice of insufflation gas can affect intraperitoneal macrophage function in the clinical setting, and possibly acid–base balance. The present study suggested no immunological advantages for the clinical use of helium as an insufflation gas. The outcomes of the present study, however, are different to those obtained from previous laboratory studies and further research is needed to confirm this outcome.     

Oncological effects of insufflation with different gases and a gasless procedure in rats

Background: The validity of using carbon dioxide (CO₂) pneumoperitoneum in laparoscopic tumor surgery has not been investigated thoroughly. The oncologic effects of a gasless procedure and insufflation with different gases were compared in rats. Methods: In all the experiments, Donryu rats were randomized to receive a gasless procedure; to receive insufflation with CO₂, helium, or air at 10 mmHg for 30 min, or to serve as control subjects without insufflation. In experiment 1, involving 60 rats, ascites hepatoma AH130 cells were inoculated intraperitoneally just before the procedures. The S-phase fraction of the intraperitoneal tumor cells was determined using a flow cytometry on day 7. In experiment 2, 60 rats injected intraperitoneally with latex particles received one of the procedures. At the end of the procedure, peritoneal macrophages were harvested to determine the number of phagocytosed particles. In experiment 3, 75 rats inoculated intraperitoneally with AH130 cells received one of the procedures for 5 consecutive days and were followed for survival analysis. Results: Experiment 1: The S-phase fraction was lower after insufflation with air or helium (p < 0.01) than with the anesthesia control condition. Insufflation with CO₂ showed a higher S-phase fraction than the gasless procedure or insufflation with air or helium ( p < 0.01). Experiment 2: The phagocytotic activity of peritoneal macrophages was increased by insufflation with helium and air, as compared with the control condition ( p < 0.01). Insufflation with CO₂ deteriorated the phagocytotic activity more than the gasless procedure ( p < 0.05) or insufflation with air or helium ( p < 0.001). Experiment 3: Insufflation with gases demonstrated shorter survival than the anesthesia control condition or the gasless procedure regardless of the gases used (p < 0.01). Conclusions: These results suggest that the choice of gases may affect the proliferation of tumor cells and the phagocytotic activity of peritoneal macrophages, insufflation itself may promote tumor spread regardless of the gases used, and the gasless procedure may be oncologically advantageous in this animal model.     

不同剂量右美托咪定对妇科腹腔镜手术围术期炎症因子的影响

目的探讨不同剂量右美托咪定对妇科腹腔镜手术围术期炎症因子的影响。方法选择2012年1月至2013年12月择期妇科腹腔镜手术患者90例,按照随机数字表法将其分为高剂量右美托咪定组(D1组)、低剂量右美托咪定组(D2组)和对照组(C组),每组30例。D1组和D2组分别于麻醉诱导后持续静脉输注右美托咪定0.5和0.2μg·kg-1·h-1,C组则以等容量生理盐水代替。记录麻醉诱导前10 min(T1)、气管插管后1 min(T2)、气腹后1 min(T3)、术毕(T4)的MAP和HR,并检测血清TNF-α、IL-6、IL-10水平。结果与T1时比较,T2~T4时D2组和C组MAP明显升高,D1组HR明显减慢,T2、T3时C组HR明显增快(P0.05)。与C组比较,T2~T4时D1组和D2组MAP明显降低,HR明显减慢(P0.05)。与T1时比较,T2~T4时三组TNF-α、IL-6和IL-10水平明显升高(P0.05)。与C组比较,T2~T4时D1组和D2组TNF-α和IL-6水平明显降低,但D1组IL-10水平明显升高,T2、T3时D2组IL-10水平明显降低(P0.05)。结论持续输注右美托咪定可减轻妇科腹腔镜手术围术期炎症反应,且0.5μg·kg-1·h-1较0.2μg·kg-1·h-1更为明显。     

Diagnostic laparoscopy for the evaluation of occult diaphragmatic injury following penetrating thoracoabdominal trauma

BACKGROUND: Occult diaphragmatic injury following penetrating thoracoabdominal trauma can be difficult to diagnose. Radiographic findings are often non-specific or absent. Undetected injuries may remain clinically silent, only to present later with life-threatening complications associated with diaphragmatic herniation. Diagnostic laparoscopy allows for the evaluation of trauma patients lacking clinical indications for a formal laparotomy. The purpose of this study was to evaluate the incidence of occult diaphragmatic injury and investigate the role of laparoscopy in patients with penetrating thoracoabdominal trauma who lack indications for exploratory laparotomy except the potential for a diaphragmatic injury. METHODS: Haemodynamically stable patients with penetrating thoracoabdominal trauma without indications for laparotomy (haemodynamic instability, evisceration, or peritonitis on exam) and evaluated with diagnostic laparoscopy to determine the presence of a diaphragmatic injury were retrospectively reviewed. Thoracoabdominal wounds were defined as wounds bounded by the nipple line over the anterior and posterior chest superiorly and the costal margin inferiorly. RESULTS: One hundred and eight patients were evaluated for penetrating thoracoabdominal injuries (80 stabs and 28 gunshots) over the study period. 22 (20%) diaphragmatic injuries were identified. These were associated with injuries to the spleen (5), stomach (3) and liver (2). There was a greater incidence of haemopneumothorax (HPTX) in patients with diaphragmatic injury (32%) compared to those without injury (20%). 29% of patients with a HPTX had a diaphragmatic injury. However, 18% of patients with a normal chest radiograph were also found to have a diaphragmatic injury. CONCLUSIONS: The incidence of diaphragmatic injury associated with penetrating thoracoabdominal trauma is high. Clinical and radiographic findings can be unreliable for detecting occult diaphragmatic injury. Diagnostic laparoscopy provides a vital tool for detecting occult diaphragmatic injury among patients who have no other indications for formal laparotomy.     

不同CO2气腹压力对各年龄段患者手术期间呼吸系统参数的影响

目的:探讨腹腔镜胆囊切除术(LC)在不同CO2气腹压力下对不同年龄段患者围手术期呼吸系统各参数的影响。方法:择期行腹腔镜胆囊切除术90例。按年龄分为3组,20~40岁为中青年组,40~60岁为中老年组,>60岁为老年组,每组30例,各组按压力不同分A组(气腹压力8~12mm Hg)、B组(气腹压力14~16mm Hg)两组,每组15例,各组间性别,体重无统计学差别。观察各年龄段在不同气腹压力下各时点(气腹前10m in、气腹后10、20、30m in、放气后5m in)呼吸系统参数(PETCO2、Paw、MVE、VCO2、VO2)的变化情况。结果:中青年组和中老年组:在A组、B组气腹注入后,呼吸系统各参数均有显著变化(P<0.01),A、B两组间比较差异无显著性(P>0.05)。老年组:在A组、B组气腹注入后,呼吸系统各参数均有显著变化(P<0.01)。A、B两组间比较差异显著(P<0.01)。结论:(1)CO2气腹时间延长,会直接影响呼吸系统各参数的变化;(2)CO2气腹压力增加,会直接影响呼吸系统各参数的变化;(3)随时间延长气腹压力的变化对于中青年组、中老年组呼吸系统参数的影响差异无显著性,如果是健康机体,肺功能正常,手术可根据需要选用任一组气腹压力;(4)老年组气腹压力的变化随时间延长对呼吸系统各参数的影响差异显著。建议最好使用低气腹压力,以8~12mm Hg为宜。     

General vs regional anaesthesia in outpatient treatment for inguinal hernias using extraperitoneal laparoscopy

Summary The treatment of inguinal hernias using laparoscopy can be performed without violating the peritoneal cavity using the totally extraperitoneal technique (TEP). This procedure is usually done with general anaesthesia. The objective of this article is to evaluate the general and regional anaesthesia techniques in extraperitoneal laparoscopic surgery for treating inguinal hernias in an outpatient surgery unit. A prospective clinical study of 131 patients with uncomplicated inguinal hernia undergoing surgery using extraperitoneal laparoscopy was completed. Two study groups were established according to the anaesthesia technique used: general (n = 90) and regional (n = 41). We analyzed clinical data (age, sex, associated diseases, prior abdominal surgery, site and hernia type), intra-operative complications (bleeding, peritoneal rupture, subcutaneous emphysema, reconversion rate, haemodynamic stability, respiratory problems and degree of satisfaction), postoperative complications (haematomas, urinary retention, post lumbar puncture headaches, nausea, vomiting and postoperative pain) and recurrence rate. General anaesthesia was used significantly more in the cases of prior infra-umbilical surgery and bilateral hernias (p < 0.05). Statistically significant differences were not shown for intra- or post-operative complications. The rate of conversion was higher for general (5.5%) than for regional anaesthesia (2.4%). Recurrence was detected only in the regional anaesthesia group. In conclusion, general anaesthesia is not required for the performance of extraperitoneal laparoscopic inguinal hernia repair; regional anaesthesia is a safe and efficient alternative.     

Hydrophilic guide wire technique to facilitate organ entrapment using a laparoscopic sack during laparoscopy

PURPOSE: Morcellation of the kidney in a LapSac (Cook Surgical, Spencer, Indiana) is reportedly safe and effective during laparoscopic nephrectomy. However, organ entrapment in a LapSac can be difficult, especially during early surgeon experience. A technique to facilitate organ entrapment is described. MATERIALS AND METHODS: We have recently used a simple technique to facilitate deployment of the LapSac in the abdomen using a hydrophilic nitinol guide wire passed through the holes in the mouth of the sack beside its drawstring. The inherent elasticity of the guide wire helps to spring open the mouth of the sack, while facilitating appropriate orientation of the sack mouth. RESULTS: This technique appears to decrease the time needed for organ entrapment because it eliminates the need to triangulate the sack to achieve organ entrapment. An additional port is usually not required during the procedure. CONCLUSIONS: The hydrophilic guide wire technique is inexpensive and effective for LapSac deployment and organ entrapment.