The Safety of Sino-implant——3-year Clinical Observation

A total of 315 women who had Sino implant Ⅱ implantation and 302 healthy women were recruited and followed-up for 3 years in order to observe the effect of Sino implant Ⅱ on myoma of uterus and ovarian cysts.The blood pressure,bodyweight,vaginal ultrasonic examination and smear of cervix were measured once a year.The results show that Sino implant Ⅱ does not influence the blood pressure and body weight obviously.There are more ovarian cysts in Sino-implant group than in the control group,but they are all found to disappear when reexamined after menstrua-tion.These ovarian cysts may be follicular cysts.There are fewer myomas of uterus in Sino-implant group than in the control group,and these myomas develop slowly.The results suggest that Sino-implant is a safe contraceptive method.     

P53 codon 72 polymorphism and ovarian cancer risk： a meta-analysis

Objective:p53 is a tumor suppressor gene and is involved in the etiology of ovarian cancer.Studies investigating the associations between the p53 codon 72 polymorphism and ovarian cancer risk showed conflicting results.We performed this meta-analysis from eligible studies to evaluate this purported relationship.Methods:This meta-analysis was performed from 9 case-control studies, including 825 ovarian cases and 1073 controls.The fixed and random effect models were used to estimate the odds ratios(ORs)for various contrasts of this polymorphism.Results:The combined results based on all studies showed that a significantly decreased risk was associated with the variant Pro/Pro genotype,compared with Arg/Pro Arg/Arg genotypes(OR,0.70;95%CI,0.51~0.95).When stratifying the studies by ethnicity,we found that individuals with the variant genotype Pro/Pro had a significantly decreased risk of ovarian cancer compared with Arg/Arg genotype(OR,0.43;95%CI,0.20~0.89)and Arg/Pro Arg/Arg genotypes(OR,0.61;95%CI, 0.37~0.99)among Africans.Conclusion:This meta-analysis suggests that the p53 codon 72 polymorphism may contribute to genetic susceptibility to ovarian cancer.More studies based on larger sample size should be performed to confirm the findings.     

A preliminary study of radioimmunoimaging by 131I-COC183B2 monoclonal antibody in patients with epithelial ovarian cancer.

Monoclonal antibody 131I-COC183B2, developed in our laboratory and proved to fit for human treatment was injected intraperitoneally or subcutaneously in 13 patients. In 8 cases with i.p. injection the disease corresponded with the image, i.e. 3 primary ovarian epithelial cancers showed positive images, 1 ovarian Krukenberg tumor was negative and the other 4 negative images included 1 uterine myoma and 3 ovarian teratomas. In the subcutaneous injection group, 4 cases had ovarian carcinoma, surgery and chemotherapy. Two negative images corresponded with the clinical status-in good health, another negative case had metastatic left supraclavicular lymph node due to ovarian mucinous adenocarcinoma. The last negative image in this group was a case of benign ovarian teratoma which was proved after surgery. The 1 positive case was waiting to be proved by a scheduled third operation. The computer scintigram calculation of T/NT was 5.35 to 13.7. The results suggest that this monoclonal antibody can be used for radioimmunoimaging for the localization of ovarian carcinoma, which is not only helpful for clinical staging and differential diagnosis but is also a good follow-up method.

     

Differential Proteomics Reveals the Potential Injury Mechanism Induced by Heavylon Radiation in Mice Ovaries

In the present study, we used a proteomics approach based on a two-dimensional electrophoresis (2-DE) reference map to investigate protein expression in the ovarian tissues of pubertal Swiss-Webster mice subjected tocarbon ion radiation (CIR). Among the identified proteins, ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) is associated with the cell cycle[1] and that it influencesproliferation in ovarian tissues.We analyzed the expression of UCH-L1 and the proliferation markerproliferation cellnuclear antigen (PCNA) following CIR using immunoblotting and immunofluorescence. The proteomics and biochemical results provide insight into the underlying mechanisms of CIR toxicity in ovarian tissues.     

Radioimmunolocalization of human malignant tumors with In-111 labeled monoclonal antibody.

An anti-human colon carcinoma monoclonal antibody 2C10 was radiolabeled with In-111 and studied in 15 patients with gastrointestinal and ovarian carcinoma. The labelling efficiency approached 100% and immunoactivity of the labeled antibody was over 75%. 2-3 mCi (1 mg) In-111-2C10 was given to the patients intravenously and scintigraphy was performed 72 hours after administration with a gamma camera. Specimens were also scanned in some of the patients. The resected tumors and remote margin were examined immunohistochemically. Positive scintigraphic images were obtained in 12/15 patients with colorectal cancer (10) and ovarian cancer (2). Negative results were seen in the two patients with gastric cancer. The scintigraphic results of 10 patients were confirmed surgically and pathologically. The remaining 5 were confirmed by endoscopy, B-ultrasonography or X-ray CT. Most patients had been definitely diagnosed before imaging except one patient with metastatic focus from ovarian cancer to colon and one with recurrent colon cancer were first detected with RIAD, showing the unique advantage the latfer. The high background radioactivity in the liver, however, is a conspicuous problem to be solved.

     

STUDY OF BRCAI GENE IN HEREDITARY BREAST AND OVARIAN CANCER

Objective. To investigate the BRCA1 gene in hereditary breast and ovarian cancer, early-onset breast cancer and sporadic ovarian cancer. Methods. The exons of 2, 11 and 20 of BRCA1 gene were analyzed, Polyrnerase chain reaction-single strand conformation analysia(PCR-SSCP) and PCR SSCP combined by restriction enzymes were used to screen for mutations. Mutations were further indentifed by sequencing. The loss of heterozygosity (LOH) were also investigated at the BRCA1 genetic loci D17S855 in 10 hereditary ovarian cancer. Results. A insertion mutation was detected in H7. “C“ was inserted ay nucleotide 797. It would result in truncation of the BRCA1 protein at cndon 277. A missen mutation was detected in an early-onset breast cancer(diagnosed at age 24). At nueleotide position 3732, the substitution of a “G“ to a “C“ in codon 1205 changes a Gly to a Arg. A missen mutation were also detected in three sporadic ovarian cancers. At nu-cleotide position 2051, the substitution of a “T“ to a “G“ in cndon 644 changes a Cys to a Trp. H3 and H7 patients show LOH. Conclusions. BRCA1 gene has an important effect in Chinese hereditary breast and ovarian cancer, its effect on early-onset breast cancer and sporadic ovarian cancer are still to be studied. BRCA1 gene is a tu-mor -suppressor gene.     

50 CASES OF DYSFUNCTIONAL UTERINE BLEEDING TREATED BY PUNCTURING THE EFFECTIVE POINTS -A New System of Acupuncture

All abnormal uterine bleeding as a re-sult of ovarian dysfunction is calleddysfunctional uterine bleeding which can beclassified from a physiopathological viewpoint into two types:ovulatory andanovulatory, belonging to the category inTCM of Beng Lou (burst out and drip, i.e.metrorrhagia and metrostaxis) or irregularmenstruation. For many years, the authorshave treated the disease by needling the ef-fective points with very satisfactory results.     

Study on Tumor Angiogenesis in Epithelial Ovarian Carcinoma

The tumor angiogenesis has been recognized asthe neovascularization induced by tumor cells, and finally the formation of capillary network to supply thetumor mass. Recently, it has been shown that thetumor angiogenesis played a key role in the occurrence, development, metastasis and prognosis of tumor. Several studies about epithelial ovarian tumorangiogenesis have been reported abroad, but their results are in controversy['J.' The purpose of this studywas to investigate tumor angiogenesis in …     

The role of SDF-1/CXCR4 axis in ovarian cancer metastasis

This study was aimed to explore the role of stromal-derived factor 1 （SDF-1）/CXC chemokine receptor 4 （CXCR4） axis in mediating the metastasis of ovarian cancer cells through activation of extracellular signal-regulated kinase-1/2 （ERK-1/2） signaling pathway. A highly metastatic ovarian cancer cell line, SKOV3, was used in the study. Intracellular calcium mobilization was detected by using laser scanning confocal fluorescence microscopy. Western blotting was used to detect the phosphorylation of ERK1/2 in SDF-1α-treated SKOV3 cells. Adhesion capability and matrix metalloproteinase （MMP） activity of ovarian cancer cells after exposure to SDF-1 a were measured by adhesion assay and gelatin zymography. The results showed that SDF-1α induced rapid intracellular calcium mobilization in SKOV3 cells, as well as the phosphorylation of ERK-1/2. The adhesion of ovarian cancer cells to fibronectin and collagen Ⅳ was increased after SDF-1α treatment. An inhibitor of ERK-1/2 signaling, PD98059, could antagonize such effects of SDF-1α. SDF-1α could also increase the secretion of active MMP-2 and MMP-9. It was concluded that the SDF-1/CXCR4 axis played a critical role in the metastasis of human ovarian cancer by increasing the adhesion capability of cancer cells and the activity of MMP-2 and MMP-9 via ERK1/2 signaling pathway.     

Endometriosis coexisting with dermoid cyst in a single ovary: a case report

Endometriosis coexisting with a dermoid cyst of the ovary is extraordinarily rare, although these both benign conditions are said to be common in women in the reproductive age group. There are only two previous case reports, which is evident from our literature review from January 1960 through January 2010. Acute abdomen is one of the greatest diagnostic challenges and easily ignored by the clinicians to exclude the possibility of gynecologic illness. A 35-year-old woman was referred by the doctor in Family clinic. She experienced a three-day period of severe right lower abdominal pain and intermittent vomiting. Ultrasonography identified a bilocular, cystic, hypoechoic, and hyperechoic tumor, 7 cm × 6 cm × 6 cm in the right adnexal region. Laparoscopic cystectomy was performed under the impression of ovarian cyst with torsion or hemorrhage. The frozen section was benign and appendiceal status was adequate. Histopathologic examination described an ovarian cyst composed of endometrial-type lining with stroma cells (endometriosis) and benign terotoma tissue with plenty of skin appendages and sebaceous glands. We report this unusual and interesting ovarian mass to remind physicians that the usage of the Endobag after cystectomy, the benefits on minimizing operative time, spilled opportunity, and postoperative complications. Laparoscopic techniques for large ovarian masses might be considered. The experience of the surgeon is also very important to prevent misdiagnosis or complication. Further follow up is mandatory for this simultaneous finding of ovarian endometriosis with coincidental dermoid cyst as a separate pathology in single ovary of such a nature. It also presents a challenge to the clinicians and to the pathologists.

     

血清CA125、CA199与CEA联合检测在卵巢癌诊断中的意义

目的探讨肿瘤标志物糖类抗原125(CA125)、糖类抗原199(CA199)、癌胚抗原(CEA)联合检测在卵巢癌诊断中的意义。方法运用化学发光法测定85例卵巢癌患者、61例卵巢良性疾病患者血清中CA125、CA199、CEA含量,并与健康对照组比较。结果 85例卵巢癌患者血清CA125、CA199、CEA含量与对照组相比明显升高,差异具有统计学意义(P<0.01);良性疾病组与正常对照组相比差异无统计学意义(P>0.05)。单检CA125敏感度71.7%,特异性93.4%,有效性80.8%;CA125、CA199、CEA联合检测敏感度提高到90.6%,有效性89.7%。结论联合检测CA125、CA199、CEA可明显提高卵巢癌的检出率,是诊断早期卵巢癌较好的实验室检测组合项目,对卵巢癌的临床诊断及判断病情、疾病的转归有重要意义。     

体外药敏试验在卵巢恶性肿瘤的临床应用

本文用生化快速氚-胸腺嘧啶核苷(~3H-TdR)掺入法对54例卵巢恶性肿瘤进行体外药敏试验,并以20例卵巢良性肿瘤作对照。结果两者对化疗药物的敏感性差异有极显著性意义(P<0.001)。在54例中,48例体外药敏试验结果与临床疗效一致者33例,符合率为68.8％。     

抗CEA单克隆抗体对卵巢癌放射免疫显像研究

用~(131)I-抗CEA单克隆抗体对22例卵巢癌患者进行放射免疫显像研究,结果20例阳性显像,2例阴性显像(假阴性),20例阳性者结果与手术中所见相符,48处转移灶共发现45处,阳性率93.8％,最小检出病灶直径1.5cm,其中2例大网膜广泛转移和1例升结肠转移为采用本方法首次发现,显示放射免疫显像对卵巢癌不仅可以定性定位,还能检出转移灶,与B超、CT等方法比较具有一定优点,有较好的临床应用价值.     

卵巢病理与功能关系的研究

卵巢活检病理检查是鉴别卵巢功能异常种类的常用方法。本文对１０例生育年龄正常卵巢的病理与功能做了对照研究，以探讨卵巢病理检查与功能的关系。结果表明，病理检查对卵巢功能的反映率仅占５０％。可见卵巢活检并不能完全反映卵巢内卵泡情况及卵巢的功能状态。因此，对卵巢性闭经患者既使活检未发现卵泡，也不能确认卵巢内无卵泡存在而放弃必要的治疗。     

增龄与卵巢匀浆超氧化物歧化酶关系的初探

为探讨卵巢组织超氧化物歧化酶（ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅＳＯＤ）活性与卵巢老化的关系，本研究对不同年龄组的３４例妇科剖腹手术病人的正常卵巢组织的ＳＯＤ活性和丙二醛（ｍａｌｏｎｄｉａｄｅｈｙｄｅ，ＭＤＡ）含量进行测定。结果显示：年龄与卵巢组织的ＳＯＤ活性呈负相关，在围绝经期ＳＯＤ活性下降最明显，但不同年龄组卵巢组织的ＭＤＡ含量无显著性差异。提示卵巢组织ＳＯＤ活性的下降可能是卵巢老化的原因之一。     

乳源免疫调节肽通过泛素-蛋白酶体途径抑制卵巢癌耐药性及其机制研究

目的 研究乳源免疫调节肽(PGPIPN)抑制卵巢癌耐药性及其机制.方法 MTT法检测PGPIPN与抗癌药顺铂(DDP)联合用药抑制人卵巢癌敏感细胞株(SKOV3)、耐药细胞株(SKOV3-DDP)和原代卵巢癌细胞的半数抑制浓度(IC50)和细胞增殖抑制率,PCR法和Western blot法检测人卵巢癌细胞株和原代卵巢癌细胞用药情况下泛素-蛋白酶体途径相关基因的表达.结果 PGPIPN和DDP联合用药细胞增殖抑制率显著高于单独DDP组或PGPIPN组,差异有统计学意义( P＜0. 05),PCR和Western blot结果表明 PG-PIPN通过调节泛素-蛋白酶体途径相关基因表达降低卵巢癌细胞的耐药性. PGPIPN促进SIAH和PSMA1 表达,降低β-catenin基因的表达(P ＜0. 05,P ＜0. 01),且有剂量依赖性.结论 PGPIPN通过泛素-蛋白酶体途径降低卵巢癌细胞对DDP的耐药性.     

AIB1蛋白在卵巢肿瘤中的表达及意义

目的:探讨AIB1蛋白在卵巢肿瘤组织中的表达及其临床意义。方法:采用免疫组化法检测44例卵巢癌组织、25例卵巢交界性肿瘤、20例卵巢良性肿瘤、20例正常卵巢组织中AIB1蛋白的表达,结合肿瘤的临床学资料及临床分期,分析其相关性。结果:卵巢癌组织中AIB1蛋白的表达显著高于卵巢交界性肿瘤、卵巢良性肿瘤和正常卵巢组织(P<0.05),在卵巢癌肿瘤组织中,AIB1蛋白的表达与肿瘤的临床分期密切相关(P<0.05),而与肿瘤的组织学类型、病理分级无显著相关性(P>0.05)。结论:AIB1蛋白的过表达可能与卵巢癌的发生发展相关。     

经阴彩超引导下穿刺活检卵巢肿瘤13例分析

目的研究经阴彩超引导下穿刺活检在晚期卵巢肿瘤诊断及治疗中的应用价值。方法有选择的对13例初步诊断为晚期卵巢恶性肿瘤的患者在阴道彩超引导下进行穿刺活检,取组织条及组织液送病理及细胞学检查。结果经阴彩超引导下穿刺活检卵巢肿瘤成功率100%,穿刺活检病理结果与术后病理结果符合率100%。结论经阴彩超引导下选择性对卵巢肿瘤进行穿刺活检,在晚期卵巢恶性肿瘤等某些特殊病例的确诊与治疗中具有无可替代的重要作用。     

超声在卵巢巧克力囊肿与盆腔炎性囊肿鉴别诊断中的价值

目的:研究并探讨超声在卵巢巧克力囊肿与盆腔炎性囊肿中的鉴别诊断价值。方法:选取中山市小榄人民医院2016年1月至2018年6月期间确诊的104例卵巢巧克力囊肿患者、24例盆腔炎性囊肿患者进行研究,所有患者均接受经阴道彩色多普勒超声检查,对阴道彩超图像进行观察,比较卵巢巧克力囊肿、盆腔炎性囊肿的超声特征。结果:超声对卵巢巧克力囊肿、盆腔炎性囊肿的诊断结果与病理诊断结果之间的一致性均良好。在超声特征方面,卵巢巧克力囊肿的超声信号偏低,其收缩末期峰值流速、舒张末期峰值流速高于盆腔炎性囊肿(P<0.05),其血流阻力指数低于盆腔炎性囊肿(P<0.05)。结论:经阴道彩色多普勒超声用于卵巢巧克力囊肿、盆腔炎性囊肿鉴别诊断中具有较高的价值,可作为卵巢巧克力囊肿、盆腔炎性囊肿的辅助诊断手段。